FastQCFastQC Report
Thu 11 Sep 2014
SDW_GOR69_1_CGATGT_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_GOR69_1_CGATGT_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12600862
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGACACTCGTTATCGATAACGGCTCTGGTATGTGCAAGGCTGGCTTCTC270390.2145805580602343No Hit
GAACGATCAGCTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCTATCA172200.13665731757081379No Hit
CTTCAGTCAACTTCCCAGCACGTGAACTTTCACGCAATGTTCGCCCAACC157760.12519778408810445No Hit
TTCAGACACTCGTTATCGATAACGGCTCTGGTATGTGCAAGGCTGGCTTC156790.12442799548157896No Hit
CGTCAAGACACTCACAGGCAAACATATCACACTCGAAGTCGAGCCAACCG133160.10567531014941675No Hit
CACAGTTCCAATTTACGAAGGCTACTCCCTTCCACACGCCATCATGAGAC127980.10156448027127034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCCGC20700.050.4533235
AGACCCG22500.046.8386544
CGGACTA10700.045.253314
CCCGCTG26150.040.307517
AACCTAC19500.036.5169145
ACCCGCT29850.035.78296
CGTTCGA21050.035.6318974
CTGTTAG44750.034.0625428
CCTACTA18350.033.8953367
ACCTACT19850.031.329126
CCGATCC21250.029.7116453
CGTGGAG30100.028.8669821
CCTCGGG3800.028.7385814-15
GGAGCGA30800.028.3596614
GCGGACT18700.027.1628993
TGCGGAC18700.027.1593382
CTGACCC19300.027.056363
CGAGCCG6150.026.2494721
AGCGATC33500.026.0741676
TACTGTC27700.025.1925164
Produced by FastQC (version 0.11.2)