FastQCFastQC Report
Thu 11 Sep 2014
SDW_GOR69_1_CGATGT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_GOR69_1_CGATGT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12600862
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAACACGAAGTTCGTTGTAGAATGTGTGGTGCCAGATCTTTTCCATAT148870.11814271118912342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGCT2300.035.1300851
CGAGCCC6000.033.64588562-63
CCCTATG6050.032.97735266-67
CTCGGTT27450.032.2054021
CTCGGGG5150.031.81570416-17
CCGGGTT5850.029.2485771
CCTCGGG5700.028.74571214-15
CCGGGAT8200.028.4014381
CGGGTCA2950.027.3895591
GCCCTAT7400.026.96121666-67
CCAGGGA12350.026.1499425
CCGCTAC15300.025.4730261
CCGGGGT2800.025.461931
GGCAGGG12700.025.4292776
AGGGACG12600.025.254179
CGGGACG1000.001648702723.764471
TCCAGGG14350.023.1673684
AGCCCTA9050.022.56976964-65
CCCGTGT2755.0931703E-1122.4682251
CAGGGAC14050.022.309848
Produced by FastQC (version 0.11.2)