FastQCFastQC Report
Thu 11 Sep 2014
SDW_G3_3_ACAGTG_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_G3_3_ACAGTG_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12274083
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAGTCAACTTCCCAGCACGTGAACTTTCACGCAATGTTCGCCCAACC181660.14800290987114884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCTAC27700.047.648735
GACCCGC22450.046.948635
ACCTACT25750.046.6474466
AGACCCG22400.046.4171944
CCTACTA27300.043.4809577
CGGACTA6700.040.3908354
CCCGCTG27250.038.5076947
CGCGGTC13000.035.7913361
CAACCTA37500.034.943034
ACCCGCT30200.034.9004146
CGAGCCG7000.033.234811
CGTTCGA16450.032.6133424
GCGGTCG14100.032.3247342
CGTACTG14050.031.7667857
CGTGGAG18600.031.65221
GGAGCGA20500.027.3281964
ACGACGC35200.027.1128467
TGGAGCG22000.026.9759773
CCATAGT10250.026.8674147
CACGGTT27500.026.0699041
Produced by FastQC (version 0.11.2)