FastQCFastQC Report
Thu 11 Sep 2014
SDW_G3_3_ACAGTG_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_G3_3_ACAGTG_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12274083
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTTTTCAGCCTTGACATCATCTTCAACACCAGCGAGAACGCCGAGTT145300.11837951560210241No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTATG3800.038.7489366-67
CCGGGCT1750.038.0271451
CCGGGAT6850.035.390231
CCCGGGA958.660536E-735.0251
CCGGGTT6350.031.4397661
CTCGGTT21150.029.6665651
CTCGGGG4700.029.30782116-17
CCTCGGG4750.028.49956114-15
AGGGACG16850.026.497579
CCTATGC5850.025.57621868-69
CACACCC7500.025.335013
GCCCTAT5950.024.74721766-67
CGAGCCC6400.023.3780762-63
GGCAGGG18950.023.3105136
CTAACCG7300.021.14650558-59
CAGGGAC20900.021.1356098
CGGGGTA2509.433279E-920.9149281
CGGGGTG1856.5202075E-620.5552141
CCGGGGT2801.5661499E-920.3716871
GACCCGA8900.020.28302692-93
Produced by FastQC (version 0.11.2)