FastQCFastQC Report
Thu 11 Sep 2014
SDW_G3_2_ACAGTG_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_G3_2_ACAGTG_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17628836
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAGTCAACTTCCCAGCACGTGAACTTTCACGCAATGTTCGCCCAACC211740.12011002881869227No Hit
CTTATGTCCACTTCCTCCCACAAGCACTTGAGGGACAAGAGAAGGTCATC184060.10440848164904365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCCGC31500.047.1717575
AGACCCG33700.044.7965134
ACCTACT33500.044.638956
AACCTAC36350.044.0122685
CCTACTA37300.040.097547
CGGACTA8950.037.6601754
CCCGCTG40150.037.4877857
ACCCGCT42000.036.0570036
CAACCTA50950.032.4251564
CGCGGTC17600.032.0984271
CGTACTG20750.028.1451197
CGAGCCG7350.027.1276041
CGTTCGA25800.025.576564
CTGACCC25650.024.8007283
GCGGTCG22800.023.944462
TACTACG66050.023.1490739
CCCTGAC10500.023.07077238-39
ACGACGC42850.022.6045257
TAGTGCG13550.022.4226883
CTTTGAC85250.022.3862381
Produced by FastQC (version 0.11.2)