FastQCFastQC Report
Thu 11 Sep 2014
SDW_G3_2_ACAGTG_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_G3_2_ACAGTG_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17628836
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTTTTCAGCCTTGACATCATCTTCAACACCAGCGAGAACGCCGAGTT183720.10421561582398292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTATG6650.035.35649566-67
CGGGTCG958.659317E-735.0257841
CCGGGTT7050.033.712761
CCTCGGG7300.029.2795414-15
CCGGGAT8500.029.0802351
CGAGCCC8200.028.9627662-63
CTCGGGG7550.027.36623616-17
GCCCTAT8750.026.87093566-67
CACACCC14050.026.3705223
CCGGGCT2704.0017767E-1122.887221
CGGGGAT9150.022.8583581
CCTATGC10600.022.62930368-69
CGGGGTT11200.022.494241
CGGGGTG2806.548362E-1122.069821
CGGGGCT5600.022.069821
CTCGGTT33300.021.6976571
CGCCCTA2005.357051E-721.3907471
CGGGGTC2457.4742275E-921.3422451
AGGGACG20850.021.1859429
CGGGCCC1604.966245E-520.7965621
Produced by FastQC (version 0.11.2)