FastQCFastQC Report
Thu 11 Sep 2014
SDW_G3_1_ACAGTG_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_G3_1_ACAGTG_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12776898
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATGTCCACTTCCTCCCACAAGCACTTGAGGGACAAGAGAAGGTCATC146230.1144487496104297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCCGC23850.051.3570255
AGACCCG26150.047.0211454
CCCGCTG28200.043.6082347
ACCCGCT31250.039.3476036
CGGACTA7000.035.945374
CCTCGGG9450.033.16020614-15
CGTTCGA26500.033.1429374
CGAGCCC11000.032.39693562-63
CCCTGAC12050.030.15119638-39
CTCGGGG10850.028.65952916-17
TCGGGGA12600.028.25843816-17
CAGGGAT25800.028.1540096
GCCCTAT13500.026.39620866-67
CCGCTGA49100.025.9190358
CCAGGGA28150.025.8036755
TATGTCC64500.025.0991953
GTCCACT68200.024.7818746
AACCTAC22350.024.2156335
ACCTACT20300.023.8546286
CCTACTA20050.023.6811897
Produced by FastQC (version 0.11.2)