FastQCFastQC Report
Thu 11 Sep 2014
SDW_G3_1_ACAGTG_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_G3_1_ACAGTG_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12776898
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTATG9650.038.39422666-67
CGAGCCC10050.036.86494462-63
CCTCGGG9250.036.1992314-15
CTCGGGG9450.034.9304416-17
CCGGGCT2600.034.734171
GCCCTAT11350.032.22504466-67
TCCAGGG22800.031.246884
CCAGGGA23700.030.4610925
AGCCCTA12450.030.33090864-65
CGGGGCA2200.030.2469791
GCCTACG13050.027.84515276-77
CCTATGC14000.026.46511368-69
CCGGGAT4700.026.2937261
CCGGGTT5450.024.419581
CTAACCG15050.024.3014258-59
TCGGGGA15450.023.97823316-17
CGGGGAA15800.023.59732418-19
CCCGGCT1852.5298687E-723.1231731
CGGGGTC2503.8016879E-1022.8148671
CTCGGTT21250.022.591191
Produced by FastQC (version 0.11.2)