##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename SDW_B7268_METR_3_GTGAAA_L004_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14097820 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18386012872912 33.0 33.0 33.0 33.0 33.0 2 32.132046160328336 33.0 33.0 33.0 33.0 33.0 3 32.10667798283706 33.0 33.0 33.0 33.0 33.0 4 35.920004795067605 37.0 37.0 37.0 33.0 37.0 5 35.92114092817187 37.0 37.0 37.0 33.0 37.0 6 35.89782597593103 37.0 37.0 37.0 33.0 37.0 7 35.94354701648908 37.0 37.0 37.0 33.0 37.0 8 35.89054328967174 37.0 37.0 37.0 33.0 37.0 9 35.85581295547822 37.0 37.0 37.0 33.0 37.0 10-11 35.81500299337061 37.0 37.0 37.0 33.0 37.0 12-13 35.783426763854266 37.0 37.0 37.0 33.0 37.0 14-15 37.80940361701313 40.0 37.0 40.0 33.0 40.0 16-17 37.806989875030325 40.0 37.0 40.0 33.0 40.0 18-19 37.752860690518105 40.0 37.0 40.0 33.0 40.0 20-21 37.680804088859134 40.0 37.0 40.0 33.0 40.0 22-23 37.60713865689873 40.0 37.0 40.0 33.0 40.0 24-25 37.50659864432941 40.0 37.0 40.0 33.0 40.0 26-27 37.44953780087985 40.0 37.0 40.0 33.0 40.0 28-29 37.36678351688417 40.0 37.0 40.0 33.0 40.0 30-31 37.295135772764866 40.0 37.0 40.0 33.0 40.0 32-33 37.20401902563658 40.0 37.0 40.0 33.0 40.0 34-35 37.18713535142313 40.0 37.0 40.0 33.0 40.0 36-37 37.23305074827172 40.0 37.0 40.0 33.0 40.0 38-39 37.20243438347205 40.0 37.0 40.0 33.0 40.0 40-41 37.142428120092326 40.0 37.0 40.0 33.0 40.0 42-43 37.03714152259002 40.0 37.0 40.0 33.0 40.0 44-45 36.923870428193865 40.0 37.0 40.0 33.0 40.0 46-47 36.81251069314263 40.0 37.0 40.0 33.0 40.0 48-49 36.643463528403686 40.0 37.0 40.0 33.0 40.0 50-51 35.64101119889458 38.5 35.0 38.5 30.0 40.0 52-53 35.77468026971546 37.0 37.0 38.5 33.0 40.0 54-55 36.58234283740323 38.5 37.0 40.0 33.0 40.0 56-57 36.58677543762085 37.0 37.0 40.0 33.0 40.0 58-59 36.36936891661264 37.0 37.0 40.0 33.0 40.0 60-61 36.08443312512148 37.0 37.0 40.0 33.0 40.0 62-63 35.822788239600165 37.0 37.0 40.0 33.0 40.0 64-65 35.559715509206384 37.0 37.0 40.0 27.0 40.0 66-67 35.26384625424356 37.0 35.0 37.0 27.0 40.0 68-69 34.9502414203047 37.0 33.0 37.0 27.0 40.0 70-71 34.73296548686251 37.0 33.0 37.0 27.0 40.0 72-73 34.508663467117614 37.0 33.0 37.0 27.0 38.5 74-75 34.32350051284525 37.0 33.0 37.0 27.0 37.0 76-77 34.139164956000286 37.0 33.0 37.0 27.0 37.0 78-79 33.95316094970712 37.0 33.0 37.0 27.0 37.0 80-81 33.75020765621919 37.0 33.0 37.0 27.0 37.0 82-83 33.592533349127734 37.0 33.0 37.0 27.0 37.0 84-85 33.45471012539527 37.0 33.0 37.0 27.0 37.0 86-87 33.320505723579956 37.0 33.0 37.0 27.0 37.0 88-89 33.183254006647836 37.0 33.0 37.0 27.0 37.0 90-91 33.08302404201501 37.0 33.0 37.0 27.0 37.0 92-93 32.953354738534046 37.0 33.0 37.0 27.0 37.0 94-95 32.87331622903399 37.0 33.0 37.0 27.0 37.0 96-97 32.76539482700162 37.0 33.0 37.0 27.0 37.0 98-99 32.676111448436714 33.0 33.0 37.0 27.0 37.0 100-101 31.935792200496245 33.0 33.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.3000653936739326 2306 2 0.3131963274765397 2306 3 0.35423940771729434 2306 4 0.39672056484455 2306 5 0.38130358935030984 2306 6 0.42786072571846745 2306 7 0.3916005333512942 2306 8 0.4059135663583078 2306 9 0.42604076032468186 2306 10-11 0.39681246152878913 2306 12-13 0.461137454635832 2306 14-15 0.6305088103827785 2306 16-17 0.5434587282669909 2306 18-19 0.5027670823045014 2306 20-21 0.5155971714399712 2306 22-23 0.43893567168562697 2306 24-25 0.5266912813095104 2306 26-27 0.4588764889988255 2306 28-29 0.4116533281229948 2306 30-31 0.4389009475252621 2306 32-33 0.4069049457517835 2306 34-35 0.39669535986116955 2306 36-37 0.5846644849551055 2306 38-39 0.42296184090373856 2306 40-41 0.37319314361614886 2306 42-43 0.31237406745425034 2306 44-45 0.2869835936986149 2306 46-47 0.277840139203569 2306 48-49 0.34548829802880476 2306 50-51 0.34849605665809236 2306 52-53 0.3813548436598708 2306 54-55 0.35589697950027954 2306 56-57 0.43207635438090364 2306 58-59 0.39408567352673884 2306 60-61 0.34269031967151875 2306 62-63 0.28318038402401413 2306 64-65 0.1909203639561241 2306 66-67 0.2818608436764194 2306 68-69 0.2972895781201075 2306 70-71 0.29979538906911785 2306 72-73 0.3127890763327059 2306 74-75 0.24760618001125323 2306 76-77 0.2454985914177925 2306 78-79 0.23075956448667512 2306 80-81 -0.04102639509189032 2306 82-83 -0.060856541859607205 2306 84-85 0.06427090933399882 2306 86-87 0.09637625707201636 2306 88-89 0.06394720206292703 2306 90-91 -0.103779404377768 2306 92-93 -0.004191817116058871 2306 94-95 0.06978869647584673 2306 96-97 -0.027518866028096056 2306 98-99 -0.14649479547932742 2306 100-101 0.05754627477070784 2307 1 0.3710006201551437 2307 2 0.3810842547986013 2307 3 0.3954944311777524 2307 4 0.48022808885976787 2307 5 0.48015536820187776 2307 6 0.4966798114485229 2307 7 0.4456856881823441 2307 8 0.4778114515645413 2307 9 0.4747528873000775 2307 10-11 0.5298823245725899 2307 12-13 0.5391951625953908 2307 14-15 0.7649339071470607 2307 16-17 0.7300173509641255 2307 18-19 0.7196303089727678 2307 20-21 0.7083511835863661 2307 22-23 0.6684712810502944 2307 24-25 0.7297070406173845 2307 26-27 0.679705501098617 2307 28-29 0.6906780816962055 2307 30-31 0.7169722340840465 2307 32-33 0.6822339962845021 2307 34-35 0.6767671001035964 2307 36-37 0.7405945417697595 2307 38-39 0.7596354856460081 2307 40-41 0.7483723466747918 2307 42-43 0.7130623006696624 2307 44-45 0.7563705450050193 2307 46-47 0.7794141666994108 2307 48-49 0.7622459922812723 2307 50-51 0.7340044692335823 2307 52-53 0.7645307996584947 2307 54-55 0.7784913252795036 2307 56-57 0.7391107563981407 2307 58-59 0.7850059210494749 2307 60-61 0.7683928685173385 2307 62-63 0.7398531804744763 2307 64-65 0.6708583683388838 2307 66-67 0.6836006594163706 2307 68-69 0.6041075513566696 2307 70-71 0.6808451160258926 2307 72-73 0.6961765687498982 2307 74-75 0.583848209696832 2307 76-77 0.5658489582475283 2307 78-79 0.638318262524777 2307 80-81 0.5836305253682141 2307 82-83 0.5980363449762862 2307 84-85 0.6437233781935845 2307 86-87 0.686206484906144 2307 88-89 0.5807058366655227 2307 90-91 0.5674654189665844 2307 92-93 0.6026181829116126 2307 94-95 0.5794409103847684 2307 96-97 0.5963167164928223 2307 98-99 0.5704224519029566 2307 100-101 0.5500185320419178 2201 1 0.25594866425718266 2201 2 0.2583079754153914 2201 3 0.26986262677036876 2201 4 0.37744457666494213 2201 5 0.2661402830067061 2201 6 0.3669247945994698 2201 7 0.36284996450606855 2201 8 0.3808628300182235 2201 9 0.37743830507443477 2201 10-11 0.4161456710956415 2201 12-13 0.4379998804385892 2201 14-15 0.48773043196287347 2201 16-17 0.48097698648194154 2201 18-19 0.5158249826932675 2201 20-21 0.5752856561378366 2201 22-23 0.5799304977734252 2201 24-25 0.6307373909223983 2201 26-27 0.5484000181819937 2201 28-29 0.5050409885217277 2201 30-31 0.5268309614821831 2201 32-33 0.4965309142695702 2201 34-35 0.5566319004606939 2201 36-37 0.6021150649900875 2201 38-39 0.5948039386467556 2201 40-41 0.6104538421213093 2201 42-43 0.5900872598400326 2201 44-45 0.5506251320579025 2201 46-47 0.603444368215925 2201 48-49 0.6353738767355352 2201 50-51 0.7420566473080612 2201 52-53 0.668135258107931 2201 54-55 0.737608625759691 2201 56-57 0.7084058443785679 2201 58-59 0.6747874783324406 2201 60-61 0.6293313959244102 2201 62-63 0.6284783163664116 2201 64-65 0.6145429773692541 2201 66-67 0.6200289377911972 2201 68-69 0.5896811645680131 2201 70-71 0.6320924406168018 2201 72-73 0.6441063894134729 2201 74-75 0.6104071683812577 2201 76-77 0.5766639737576256 2201 78-79 0.628273804413439 2201 80-81 0.618632081006318 2201 82-83 0.6517726033385927 2201 84-85 0.620635193033614 2201 86-87 0.5761477952887546 2201 88-89 0.6460815920287857 2201 90-91 0.6380190175083769 2201 92-93 0.6440847095909774 2201 94-95 0.6023403394206426 2201 96-97 0.520243088053796 2201 98-99 0.5601151486415361 2201 100-101 0.5323547811737086 1101 1 0.27535861164761855 1101 2 0.2851961281483568 1101 3 0.32560336376369037 1101 4 0.38262840508045315 1101 5 0.34415959333544066 1101 6 0.38563450056066273 1101 7 0.3840807036888947 1101 8 0.3949185526582042 1101 9 0.41231065032658165 1101 10-11 0.42460070198969646 1101 12-13 0.4569356896963015 1101 14-15 0.5806408870480979 1101 16-17 0.6097058541308442 1101 18-19 0.5954535235446059 1101 20-21 0.5954048235803739 1101 22-23 0.6514718091919889 1101 24-25 0.6509347225182296 1101 26-27 0.6165752484494931 1101 28-29 0.5807120289171479 1101 30-31 0.5396444286021662 1101 32-33 0.5761895023633556 1101 34-35 0.6386328456620447 1101 36-37 0.7005358482869681 1101 38-39 0.6212985633533847 1101 40-41 0.6016203508796778 1101 42-43 0.5404282378554441 1101 44-45 0.6079599802413895 1101 46-47 0.6664354206460388 1101 48-49 0.6796414043290753 1101 50-51 0.5940905955570344 1101 52-53 0.6438400222715615 1101 54-55 0.7536190781528447 1101 56-57 0.6864680272516281 1101 58-59 0.6059229025211792 1101 60-61 0.6972391604612227 1101 62-63 0.6843705231830768 1101 64-65 0.6354316902710622 1101 66-67 0.6504928460669319 1101 68-69 0.6184954977680874 1101 70-71 0.5670815322736829 1101 72-73 0.6545838680192801 1101 74-75 0.561326798130203 1101 76-77 0.5799889082045624 1101 78-79 0.6593559858244049 1101 80-81 0.6857512064574607 1101 82-83 0.6416689119426451 1101 84-85 0.5552505096344333 1101 86-87 0.6308944969437889 1101 88-89 0.67155249573036 1101 90-91 0.710188218759761 1101 92-93 0.6739057849316126 1101 94-95 0.6580773573498746 1101 96-97 0.6889200121665979 1101 98-99 0.6576488926154553 1101 100-101 0.6699783891605264 2304 1 0.03677701576229708 2304 2 0.07679246189618993 2304 3 0.07523799338753889 2304 4 0.11293488323428136 2304 5 0.08265478422254091 2304 6 0.1705486915882375 2304 7 0.05832534407213785 2304 8 0.10151306740276311 2304 9 0.11428543796662183 2304 10-11 0.10116026066445727 2304 12-13 0.16823522368097343 2304 14-15 0.21748597441320783 2304 16-17 0.1691833602014512 2304 18-19 0.02081785602990749 2304 20-21 0.20315194421814908 2304 22-23 0.18043407696332991 2304 24-25 0.22924568918679 2304 26-27 0.21457016634552417 2304 28-29 0.10913277772797869 2304 30-31 0.08113193024100696 2304 32-33 0.17897898394179634 2304 34-35 0.2389016992186015 2304 36-37 0.27992235569666235 2304 38-39 0.2895335463380704 2304 40-41 0.27141356413255124 2304 42-43 0.23453880007927097 2304 44-45 0.23518421194211214 2304 46-47 0.25132143750588654 2304 48-49 0.2076005142882451 2304 50-51 0.2588485095098392 2304 52-53 0.3159749562340508 2304 54-55 0.30021661074659534 2304 56-57 0.33043565808375064 2304 58-59 0.23474871927566454 2304 60-61 0.291413111341015 2304 62-63 0.32623458591047694 2304 64-65 0.31085922749714 2304 66-67 0.3391940641431148 2304 68-69 0.29298219163264605 2304 70-71 0.3652981042931387 2304 72-73 0.34174902522249084 2304 74-75 0.2587806925824694 2304 76-77 0.3828184950096798 2304 78-79 0.3149907865713857 2304 80-81 0.36734000811666334 2304 82-83 0.3810113017287122 2304 84-85 0.4047566305663821 2304 86-87 0.40894332706040615 2304 88-89 0.42871079723114036 2304 90-91 0.389429091107246 2304 92-93 0.33249016367640394 2304 94-95 0.30659900367621873 2304 96-97 0.3694199655903674 2304 98-99 0.3716004426995596 2304 100-101 0.39400300957128565 2202 1 0.17232333041851433 2202 2 0.20126772563958895 2202 3 0.17510431453123232 2202 4 0.14798432494507807 2202 5 0.23526582725771306 2202 6 0.2522421370833072 2202 7 0.24411589204930806 2202 8 0.27892585039484885 2202 9 0.2508773974169145 2202 10-11 0.2516638563944511 2202 12-13 0.3160284950520733 2202 14-15 0.42469429894127586 2202 16-17 0.41582091219012085 2202 18-19 0.4077145030518352 2202 20-21 0.4706663138596525 2202 22-23 0.4758373605953281 2202 24-25 0.5296300266253269 2202 26-27 0.39476047049134877 2202 28-29 0.4105457554030494 2202 30-31 0.4842879123246533 2202 32-33 0.4976758918402808 2202 34-35 0.4822292282694107 2202 36-37 0.5239742636554467 2202 38-39 0.547363241787572 2202 40-41 0.5041480498675313 2202 42-43 0.4698831184320511 2202 44-45 0.48989739033418545 2202 46-47 0.5635854682192303 2202 48-49 0.5855945556248159 2202 50-51 0.6255789114637054 2202 52-53 0.6227362652697366 2202 54-55 0.6113092860142473 2202 56-57 0.6006133100935429 2202 58-59 0.5907081962407759 2202 60-61 0.6136497476670044 2202 62-63 0.5668999927645046 2202 64-65 0.5408443467026984 2202 66-67 0.5463401797669718 2202 68-69 0.5080700284149131 2202 70-71 0.5050603551735406 2202 72-73 0.5702903208695105 2202 74-75 0.5723795161380707 2202 76-77 0.5668191862799361 2202 78-79 0.5942335106193042 2202 80-81 0.5567683938177836 2202 82-83 0.6087729056681326 2202 84-85 0.5875725778424084 2202 86-87 0.5705510936357499 2202 88-89 0.6072558313967491 2202 90-91 0.5296965557475914 2202 92-93 0.5643472492451806 2202 94-95 0.5943629954800613 2202 96-97 0.6011142065509532 2202 98-99 0.579168276431389 2202 100-101 0.6296235531859864 1102 1 0.24837136702453932 1102 2 0.28911840343045725 1102 3 0.27896126610330896 1102 4 0.3710262431259892 1102 5 0.3199775733107586 1102 6 0.2906904268995163 1102 7 0.3152008734714258 1102 8 0.33145749523714585 1102 9 0.32231315854983933 1102 10-11 0.38595971162642684 1102 12-13 0.3842222690213859 1102 14-15 0.5091328612446233 1102 16-17 0.4919533220913834 1102 18-19 0.47749302130497284 1102 20-21 0.5222333938442887 1102 22-23 0.508962159799367 1102 24-25 0.5139840350504556 1102 26-27 0.49181501426841123 1102 28-29 0.4888178082654875 1102 30-31 0.48612803925370685 1102 32-33 0.4898365929663058 1102 34-35 0.49294373658544544 1102 36-37 0.4515692726044236 1102 38-39 0.5057184717197813 1102 40-41 0.5210085249419691 1102 42-43 0.5254674639010872 1102 44-45 0.534777173231852 1102 46-47 0.5464604937949744 1102 48-49 0.5635431925707692 1102 50-51 0.5258745531699986 1102 52-53 0.5536381426596932 1102 54-55 0.6401896545480028 1102 56-57 0.5986892230212391 1102 58-59 0.548677958112556 1102 60-61 0.5686675112868329 1102 62-63 0.6073819671078198 1102 64-65 0.6214220995247643 1102 66-67 0.4785958412760962 1102 68-69 0.5360239077396187 1102 70-71 0.5574617750592452 1102 72-73 0.5967796886085353 1102 74-75 0.63177301888269 1102 76-77 0.5280517570806538 1102 78-79 0.5775154881583902 1102 80-81 0.6316307088686628 1102 82-83 0.577859774674927 1102 84-85 0.5812929523146053 1102 86-87 0.6033836876824168 1102 88-89 0.5584838066341149 1102 90-91 0.6334565369932292 1102 92-93 0.710318464755268 1102 94-95 0.6931162314040265 1102 96-97 0.5926995265114456 1102 98-99 0.6341775718037042 1102 100-101 0.668536597937134 2305 1 0.18187393029236176 2305 2 0.2151695357090233 2305 3 0.229287000226698 2305 4 0.24278205639070194 2305 5 0.2543564615787446 2305 6 0.28125117242542075 2305 7 0.2789167862054214 2305 8 0.2946968762951272 2305 9 0.2657653265855586 2305 10-11 0.3096249332220822 2305 12-13 0.3255585288230165 2305 14-15 0.4145218737180585 2305 16-17 0.42663721505095253 2305 18-19 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quality scores pass #Quality Count 2 3554.0 3 1827.0 4 1297.0 5 1682.0 6 1193.0 7 3290.0 8 6066.0 9 8856.0 10 12315.0 11 17670.0 12 24285.0 13 29611.0 14 33453.0 15 36126.0 16 39195.0 17 42444.0 18 45527.0 19 48508.0 20 52185.0 21 56731.0 22 61152.0 23 67841.0 24 74806.0 25 83850.0 26 95083.0 27 110160.0 28 130149.0 29 158670.0 30 196385.0 31 250401.0 32 333918.0 33 462398.0 34 671970.0 35 1056511.0 36 2048395.0 37 5113434.0 38 2715176.0 39 1706.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 21.420123046200697 20.008977071565713 12.538974861006192 46.0319250212274 2 25.47350420389309 29.800501398081515 30.08034472554744 14.645649672477953 3 18.312228826874748 31.838905160478582 26.301925436498543 23.54694057614813 4 20.010512395197956 37.076773757406585 20.356368636455507 22.556345210939956 5 23.500038669129193 39.30997244913236 19.076304480013736 18.113684401724715 6 17.22968132888306 41.7944942376565 21.40549906380057 19.570325369659873 7 16.87860783485019 18.84850917264343 39.92548613395533 24.347396858551047 8 18.306461280587584 24.923343619955553 26.94766399702807 29.822531102428794 9 20.151021047241844 25.129266027319442 25.688539886190537 29.03117303924818 10-11 20.745482106741747 34.539899253746434 21.065902232685563 23.64871640682625 12-13 19.604903815945132 27.92668112163883 27.148136349201007 25.32027871321503 14-15 20.029952713638625 29.678285510589497 26.097052385520204 24.194709390251674 16-17 20.8646354915211 29.972125360853894 24.970841039321673 24.19239810830333 18-19 20.778116536659514 30.43987340188584 25.036356730031144 23.745653331423505 20-21 20.94273863669113 30.8359906057417 24.55018379792833 23.671086959638846 22-23 21.00774124266444 30.69754313609616 24.781705745460695 23.513009875778707 24-25 21.004432975881926 30.779863868528963 24.99181435413133 23.223888801457786 26-27 21.249185155902133 31.65874551052541 24.591445520561088 22.500623813011373 28-29 21.63708441722312 30.632675740993733 24.48333374151123 23.246906100271918 30-31 21.185839717953957 30.689156422276593 24.24545322708314 23.879550632686307 32-33 21.591555317700646 30.836989003848153 24.687962726535684 22.883492951915517 34-35 21.503015172696585 30.507961328095828 24.28088997288957 23.70813352631802 36-37 21.140180032910433 29.447048570773244 25.12710787529763 24.285663521018698 38-39 22.196640214081288 29.523426360047537 24.745995215521475 23.533938210349692 40-41 21.540065985107642 29.36714670412968 24.519474688023102 24.573312622739575 42-43 22.6721875200578 29.590708687178967 24.16322051169615 23.573883281067083 44-45 21.525180770521867 30.134240518833987 24.643322048545006 23.697256662099143 46-47 21.9492144998513 29.823841641698806 24.541909772898016 23.68503408555188 48-49 21.048709357420528 29.71270163616561 24.898103988285484 24.340485018128376 50-51 21.28751359879439 30.00981396007863 25.025782813580665 23.676889627546316 52-53 21.579768371033314 29.707640594118224 24.370453228932966 24.342137805915495 54-55 20.94100374063731 30.192308170283326 24.832555439369543 24.03413264970982 56-57 21.227884957041667 30.255036090454922 25.024572269953705 23.492506682549706 58-59 21.70413692962366 29.85532904390678 24.605551373869407 23.834982652600154 60-61 21.060150366596027 30.094555659767213 25.2766784069002 23.56861556673656 62-63 21.77295393056505 30.14918868495384 24.672906660324674 23.404950724156436 64-65 22.1280404371861 29.371399402855854 24.715020041625678 23.78554011833237 66-67 21.66972998515623 29.529367535258523 25.066208552029558 23.734693927555686 68-69 21.518154202561753 29.671769465259768 25.08308789408237 23.726988438096104 70-71 21.07530916308771 29.51295776332762 24.71790906116402 24.693824012420652 72-73 20.573256210754998 29.518217066513074 25.603874546672856 24.304652176059072 74-75 21.439927940857302 29.673755967051534 25.184952320403255 23.701363771687912 76-77 21.011932954215617 29.50238509232605 24.976551048617505 24.509130904840827 78-79 20.26464218086137 29.52796711233149 25.636423474215707 24.570967232591432 80-81 21.321184073500586 29.975071127707476 25.145072828452363 23.558671970339578 82-83 21.618151367307977 29.767326438289416 24.803768162506003 23.810754031896604 84-85 20.820442490584483 30.164289793186178 25.341945117449637 23.6733225987797 86-87 21.663018489306587 30.41473818202168 24.891293262895175 23.03095006577656 88-89 21.559419419796875 29.39321580826155 25.497218384881172 23.550146387060405 90-91 20.51335863410082 29.992206399784532 26.126316539179946 23.368118426934707 92-93 21.250348257116297 29.738343263877237 25.3169188589774 23.694389620029067 94-95 21.450608160069045 30.084206068607887 25.00952444463051 23.455661326692553 96-97 20.10566650565875 30.45717938703782 25.7715223089004 23.66563179840303 98-99 21.218008594771604 30.251442665396226 24.7443677598623 23.78618097996987 100-101 20.284194041246895 30.647925306001056 25.079385566406597 23.988495086345456 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1734.0 1 1597.5 2 1376.5 3 1212.5 4 1144.0 5 1150.0 6 1200.5 7 1226.5 8 1203.0 9 1197.5 10 1208.0 11 1282.5 12 1392.0 13 1440.5 14 1544.5 15 1751.5 16 2046.5 17 2427.5 18 2878.0 19 3711.5 20 5247.5 21 7323.5 22 10895.0 23 15946.5 24 22046.0 25 30538.5 26 42770.5 27 59036.5 28 79831.0 29 104914.5 30 134137.0 31 170842.5 32 213791.0 33 253638.5 34 295577.5 35 332292.5 36 355769.0 37 379704.5 38 397454.5 39 411059.0 40 432234.5 41 465820.5 42 500834.5 43 515621.0 44 518408.0 45 544142.5 46 602117.0 47 651959.0 48 672090.5 49 686597.0 50 700232.5 51 706237.0 52 732739.0 53 708192.5 54 629140.0 55 573278.5 56 436707.5 57 261905.0 58 157731.5 59 103704.5 60 69493.0 61 35667.0 62 17588.0 63 9226.0 64 4320.0 65 2289.5 66 1466.5 67 886.5 68 573.0 69 394.0 70 296.0 71 236.5 72 187.0 73 157.0 74 127.0 75 100.5 76 83.0 77 63.0 78 49.0 79 47.0 80 41.5 81 37.5 82 29.5 83 22.5 84 17.5 85 14.5 86 11.0 87 7.0 88 7.0 89 5.5 90 3.5 91 2.0 92 2.5 93 2.0 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.045148824428174 2 0.01725798740514491 3 0.021577804227887718 4 0.014746960877639237 5 0.027592918621460624 6 0.03690641531811301 7 0.055505035530316035 8 0.061335724246727515 9 0.06141375049475735 10-11 0.05368915193980346 12-13 0.07046479526621847 14-15 0.06819494077807774 16-17 0.06149177674278718 18-19 0.06597119270922738 20-21 0.0714011102425765 22-23 0.0807678066537947 24-25 0.09728099805501844 26-27 0.09271291589763524 28-29 0.07678492135663528 30-31 0.07228777215200649 32-33 0.07661468227002473 34-35 0.08319016699035738 36-37 0.09383365655115472 38-39 0.1033599521060703 40-41 0.0984868582518432 42-43 0.09592972530504716 44-45 0.10428917378715291 46-47 0.10082055239746288 48-49 0.10000837008842503 50-51 0.10150860203918054 52-53 0.12168193380253117 54-55 0.11345725793065879 56-57 0.10549148733633995 58-59 0.10345925823992647 60-61 0.1128011281176806 62-63 0.11364523025545793 64-65 0.11440066620229228 66-67 0.12414330726310877 68-69 0.17677555820687169 70-71 0.1462779351701185 72-73 0.1220401452139409 74-75 0.12174932010764784 76-77 0.12223875748165319 78-79 0.11224785108619631 80-81 0.11175486706455325 82-83 0.09953666595260828 84-85 0.09943026652347668 86-87 0.11928085335179482 88-89 0.11209889188541207 90-91 0.10719033155480776 92-93 0.1332298185109471 94-95 0.12020652838523971 96-97 0.12602657715873802 98-99 0.12506898229655367 100-101 0.14192974516627394 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.409782E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.807366185307956 #Duplication Level Percentage of deduplicated Percentage of total 1 72.3609838538522 18.674464078455205 2 11.365409709453418 5.866225804358378 3 4.51016168736809 3.491861826625642 4 2.7134537572985074 2.801083789660093 5 1.4361057771393648 1.8531053835735924 6 1.1105994448432062 1.7196987934961012 7 0.8904141535951603 1.6085470882887944 8 0.6548813039385893 1.3520609294924109 9 0.5774914203503025 1.3413179298468476 >10 3.6118016067467007 18.08384413920526 >50 0.4045296223169712 7.382993981830451 >100 0.31001733807655113 16.074518318195956 >500 0.03341972027526185 5.866934561925267 >1k 0.01899128114500212 9.281884221410529 >5k 0.0012667587594076735 2.3779038317302805 >10k+ 4.7256484112557454E-4 2.223555321905416 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTGGAACGATCAGCTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCT 41872 0.29701045977321316 No Hit CTGGAACGATCAGCTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCTA 37982 0.26941754115175254 No Hit CTGAAGAAGACGTTCAGACACTCGTTATCGATAACGGCTCTGGTATGTGC 34906 0.24759856488449986 No Hit CGTTCAGACACTCGTTATCGATAACGGCTCTGGTATGTGCAAGGCTGGCT 19071 0.13527623419791146 No Hit CGTGGAGCGATCAACTTGTCCAACTCAAGACAGAATCAACAGGCTGCGCT 18463 0.1309635106704441 No Hit CTTACATCTTCAGTCAACTTCCCAGCACGTGAACTTTCACGCAATGTTCG 17761 0.1259840173870854 No Hit CTGAAGAAGACGTTCAGACACTCGTTATCGATAACGGCTCCGGCATGTGC 15817 0.11219465137163051 No Hit AGAAGACGTTCAGACACTCGTTATCGATAACGGCTCTGGTATGTGCAAGG 14370 0.1019306531080692 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.560524960596745E-4 0.0 0.0 2 2.3407874408951172E-4 0.0 0.0 3 9.505015669089263E-4 0.0 0.0 4 0.002567772889709189 0.0 0.0 5 0.004071551488102416 0.0 7.093295275439749E-6 6 0.006625137787260725 0.0 7.093295275439749E-6 7 0.007128761751816947 0.0 7.093295275439749E-6 8 0.008228222519510109 0.0 7.093295275439749E-6 9 0.008973018523431282 0.0 7.093295275439749E-6 10-11 0.010583196550956107 0.0 7.093295275439749E-6 12-13 0.012941717230039822 0.0 7.093295275439749E-6 14-15 0.015356984271327057 0.0 7.093295275439749E-6 16-17 0.017637478702380936 0.0 7.093295275439749E-6 18-19 0.01968744103698302 0.0 7.093295275439749E-6 20-21 0.021404018493639442 0.0 7.093295275439749E-6 22-23 0.02287233061565547 0.0 7.093295275439749E-6 24-25 0.024553441595934693 0.0 7.093295275439749E-6 26-27 0.025525223048669936 0.0 7.093295275439749E-6 28-29 0.026436711491563944 0.0 7.093295275439749E-6 30-31 0.0270715614187158 7.093295275439749E-6 7.093295275439749E-6 32-33 0.027610651859649223 7.093295275439749E-6 7.093295275439749E-6 34-35 0.028217128605699318 7.093295275439749E-6 7.093295275439749E-6 36-37 0.028890991656866094 7.093295275439749E-6 1.0639942913159625E-5 38-39 0.0294442686883504 1.0639942913159625E-5 2.1279885826319246E-5 40-41 0.0299088795288917 1.4186590550879499E-5 2.1279885826319246E-5 42-43 0.030295464121403166 1.4186590550879499E-5 2.1279885826319246E-5 44-45 0.030653675532812875 1.4186590550879499E-5 2.1279885826319246E-5 46-47 0.03109345983989014 1.4186590550879499E-5 2.1279885826319246E-5 48-49 0.03161836369027268 1.4186590550879499E-5 2.1279885826319246E-5 50-51 0.03214326754065522 1.4186590550879499E-5 2.8373181101758997E-5 52-53 0.03259723843828337 1.4186590550879499E-5 2.8373181101758997E-5 54-55 0.033122142288665904 1.4186590550879499E-5 3.5466476377198745E-5 56-57 0.033696699205976524 1.4186590550879499E-5 3.5466476377198745E-5 58-59 0.034132936865416075 1.4186590550879499E-5 3.5466476377198745E-5 60-61 0.03467557395398721 1.4186590550879499E-5 3.5466476377198745E-5 62-63 0.03537781018625574 1.7733238188599372E-5 3.5466476377198745E-5 64-65 0.036072953123248847 2.1279885826319246E-5 3.5466476377198745E-5 66-67 0.03697380162322969 2.1279885826319246E-5 3.5466476377198745E-5 68-69 0.038005876085806176 2.1279885826319246E-5 3.5466476377198745E-5 70-71 0.03910888350113706 2.1279885826319246E-5 3.5466476377198745E-5 72-73 0.04046370289874605 2.482653346403912E-5 3.5466476377198745E-5 74-75 0.04195684155422612 2.8373181101758997E-5 3.5466476377198745E-5 76-77 0.04385429804040625 3.1919828739478874E-5 3.5466476377198745E-5 78-79 0.04609223269980749 3.5466476377198745E-5 3.5466476377198745E-5 80-81 0.048759311723372833 3.5466476377198745E-5 3.5466476377198745E-5 82-83 0.05252230486699362 3.9013124014918615E-5 3.5466476377198745E-5 84-85 0.057026547366897856 4.255977165263849E-5 3.5466476377198745E-5 86-87 0.06277566318764177 4.255977165263849E-5 3.5466476377198745E-5 88-89 0.07000727772095261 4.965306692807824E-5 3.5466476377198745E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGGAG 4210 0.0 58.978985 1 TGGAGCG 4755 0.0 51.720184 3 GGAGCGA 4815 0.0 50.976727 4 GTGGAGC 5325 0.0 46.716396 2 GAGCGAT 5655 0.0 43.90863 5 AGCGATC 5985 0.0 41.249332 6 CCTGACG 2630 0.0 38.089424 6 ACCTACT 2785 0.0 38.015205 6 GTCTGAG 2225 0.0 37.127605 1 TGGAACG 15100 0.0 36.189217 3 GACGTAC 2845 0.0 35.715267 9 AACCTAC 3190 0.0 35.57034 5 CCTACTA 2970 0.0 35.172195 7 GGAACGA 16180 0.0 33.68535 4 GCGATCA 7365 0.0 33.524616 7 ACCTGAC 3105 0.0 33.3331 5 GACCTGA 3090 0.0 32.880093 4 CTGACGT 3150 0.0 32.10719 7 CGTACTG 1410 0.0 31.99173 7 CCGATCC 3055 0.0 31.236734 3 >>END_MODULE