FastQCFastQC Report
Thu 11 Sep 2014
SDW_B7268_METR_3_GTGAAA_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_B7268_METR_3_GTGAAA_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14097820
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGATCTTTTCCATATCATCCCAGTTGTTGACGATACCGTGTTCAATT184290.13072233863107913No Hit
GTCAACACGAAGTTCGTTGTAGAATGTGTGGTGCCAGATCTTTTCCATAT179960.12765094177681371No Hit
CTTGATTTCATTACCTTCTTTCTTAACGAAGAAAAGAGCTGGAATTGAAG167950.11913189415101058No Hit
CTCTGTTGGGTCAACACGAAGTTCGTTGTAGAATGTGTGGTGCCAGATCT143480.10177460061200952No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCCC608.264296E-539.608671
CTCGGTT23800.038.743271
CGGGGCA3600.036.9680941
CGGGCTA1850.035.9689521
CCCGCGT1502.5465852E-1134.855631
CCGCTAT11450.034.0392461
CGGGGCT4850.030.3802591
CCCGGTA2002.3646862E-1128.5182441
CCCGGCC856.386849E-427.959061
GTCGGGG2053.092282E-1127.8226761
CATTACC61350.026.633449
CGGGACG1257.361952E-626.6170271
TCATTAC70200.024.4933768
CCGGGTA3950.024.0660271
CGGGGAT6450.023.5809761
CCCGGGA1852.530196E-723.1228981
CGGGGTA3500.023.0861971
GTCGGGT2503.8016879E-1022.8145941
CCCGTTT4600.022.7319321
CTCGGGA4600.022.7319321
Produced by FastQC (version 0.11.2)