##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename SDW_B7268_METR_2_GTGAAA_L003_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11622325 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.0953676652477 33.0 33.0 33.0 33.0 33.0 2 32.057034887597794 33.0 33.0 33.0 33.0 33.0 3 32.042798149251546 33.0 33.0 33.0 33.0 33.0 4 35.803710101034 37.0 37.0 37.0 33.0 37.0 5 35.80135996885305 37.0 37.0 37.0 33.0 37.0 6 35.79364344053363 37.0 37.0 37.0 33.0 37.0 7 35.81290206563661 37.0 37.0 37.0 33.0 37.0 8 35.77963479768463 37.0 37.0 37.0 33.0 37.0 9 35.71884214217035 37.0 37.0 37.0 33.0 37.0 10-11 35.702704321209396 37.0 37.0 37.0 33.0 37.0 12-13 35.69511874775486 37.0 37.0 37.0 33.0 37.0 14-15 37.6504623214374 40.0 37.0 40.0 33.0 40.0 16-17 37.62597303035322 40.0 37.0 40.0 33.0 40.0 18-19 37.570554815839344 40.0 37.0 40.0 33.0 40.0 20-21 37.52406166580267 40.0 37.0 40.0 33.0 40.0 22-23 37.46560946282263 40.0 37.0 40.0 33.0 40.0 24-25 37.37565297821219 40.0 37.0 40.0 33.0 40.0 26-27 37.2674847330461 40.0 37.0 40.0 33.0 40.0 28-29 37.215744225015214 40.0 37.0 40.0 33.0 40.0 30-31 37.11255630865597 40.0 37.0 40.0 33.0 40.0 32-33 37.00462476311753 40.0 37.0 40.0 33.0 40.0 34-35 36.99935120554622 40.0 37.0 40.0 33.0 40.0 36-37 37.10022740716681 40.0 37.0 40.0 33.0 40.0 38-39 37.042736199512575 40.0 37.0 40.0 33.0 40.0 40-41 36.935077146784316 40.0 37.0 40.0 33.0 40.0 42-43 36.796079140791534 40.0 37.0 40.0 33.0 40.0 44-45 36.6967108990671 40.0 37.0 40.0 33.0 40.0 46-47 36.61210497899516 40.0 37.0 40.0 33.0 40.0 48-49 36.46638009176306 40.0 37.0 40.0 33.0 40.0 50-51 35.49201175324214 38.5 35.0 38.5 30.0 40.0 52-53 35.581056329090785 37.0 37.0 38.5 30.0 40.0 54-55 36.44180759873777 37.0 37.0 40.0 33.0 40.0 56-57 36.451861008877316 37.0 37.0 40.0 33.0 40.0 58-59 36.24756638624372 37.0 37.0 40.0 33.0 40.0 60-61 35.9963076664953 37.0 37.0 40.0 30.0 40.0 62-63 35.723562626238724 37.0 37.0 40.0 27.0 40.0 64-65 35.47307345991443 37.0 37.0 40.0 27.0 40.0 66-67 35.18841927067089 37.0 35.0 37.0 27.0 40.0 68-69 34.868254329490874 37.0 33.0 37.0 27.0 40.0 70-71 34.63196438750422 37.0 33.0 37.0 27.0 40.0 72-73 34.413812898882114 37.0 33.0 37.0 27.0 38.5 74-75 34.17224544142415 37.0 33.0 37.0 27.0 37.0 76-77 33.94129199622279 37.0 33.0 37.0 27.0 37.0 78-79 33.78187230179848 37.0 33.0 37.0 27.0 37.0 80-81 33.58617342055054 37.0 33.0 37.0 27.0 37.0 82-83 33.43553566089401 37.0 33.0 37.0 27.0 37.0 84-85 33.28685891162053 37.0 33.0 37.0 27.0 37.0 86-87 33.12887029918713 37.0 33.0 37.0 27.0 37.0 88-89 33.01810132654181 37.0 33.0 37.0 27.0 37.0 90-91 32.901434265519164 37.0 33.0 37.0 27.0 37.0 92-93 32.771633343586586 37.0 33.0 37.0 27.0 37.0 94-95 32.6883360687298 35.0 33.0 37.0 27.0 37.0 96-97 32.57473143282433 33.0 33.0 37.0 22.0 37.0 98-99 32.44364995816242 33.0 33.0 37.0 22.0 37.0 100-101 31.645338690838535 33.0 30.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.30809967934734317 2306 2 0.29808009561487836 2306 3 0.33653621043898596 2306 4 0.4153447184540511 2306 5 0.39760885724199824 2306 6 0.3973352193007216 2306 7 0.42305494815331457 2306 8 0.42003952564660096 2306 9 0.3983782029527987 2306 10-11 0.4279324940945557 2306 12-13 0.40974761665415116 2306 14-15 0.5666854229932881 2306 16-17 0.4858795708320329 2306 18-19 0.5482184819926701 2306 20-21 0.5097538090624667 2306 22-23 0.4331294399395844 2306 24-25 0.453226068507675 2306 26-27 0.4485781085003353 2306 28-29 0.3650137116113257 2306 30-31 0.406093741826389 2306 32-33 0.3380513084660848 2306 34-35 0.3911420121966387 2306 36-37 0.29124002560367757 2306 38-39 0.3055417546921717 2306 40-41 0.35314625116537 2306 42-43 0.3258343733427793 2306 44-45 0.30495576398850943 2306 46-47 0.25200328431487407 2306 48-49 0.17987557157407963 2306 50-51 0.1798910213337166 2306 52-53 0.1518434443937906 2306 54-55 0.29934987010644676 2306 56-57 0.2347009295496818 2306 58-59 0.260239635297161 2306 60-61 0.22671924531490362 2306 62-63 0.328740554598852 2306 64-65 0.13740576930047865 2306 66-67 0.17792597987113368 2306 68-69 -0.008417173743801243 2306 70-71 0.002539520145013796 2306 72-73 -0.012202082933868041 2306 74-75 0.08250146065309849 2306 76-77 0.06481114932044107 2306 78-79 0.1251975305170845 2306 80-81 0.061521600269507815 2306 82-83 -0.13558247037137505 2306 84-85 -0.008178124657902686 2306 86-87 -0.014675038642216975 2306 88-89 -0.025632517692677936 2306 90-91 -0.03979240103034698 2306 92-93 -0.05288659030204457 2306 94-95 -0.024372032590996184 2306 96-97 -0.1060561647591669 2306 98-99 -0.31541958853187424 2306 100-101 -0.2504565692723446 2307 1 0.3185392026394709 2307 2 0.292882153181111 2307 3 0.2690633861117604 2307 4 0.3845772277275046 2307 5 0.33045228614585653 2307 6 0.41618350900257894 2307 7 0.4300723428463016 2307 8 0.3734533918848797 2307 9 0.4479203319945668 2307 10-11 0.39851268388238026 2307 12-13 0.4396987979148932 2307 14-15 0.5418986175006637 2307 16-17 0.5925718289145934 2307 18-19 0.5650152775442479 2307 20-21 0.5654843654614439 2307 22-23 0.48501550039122066 2307 24-25 0.480280801014338 2307 26-27 0.48464385022239753 2307 28-29 0.5051271358023044 2307 30-31 0.511097445330897 2307 32-33 0.49788783159144856 2307 34-35 0.5379213398876388 2307 36-37 0.5399069249499675 2307 38-39 0.5559762477344492 2307 40-41 0.5454748844090318 2307 42-43 0.6457067348651933 2307 44-45 0.6046958008946035 2307 46-47 0.5598084119467472 2307 48-49 0.5808328891837178 2307 50-51 0.5563758339040916 2307 52-53 0.5806576595318731 2307 54-55 0.6596074692837561 2307 56-57 0.5543625626063218 2307 58-59 0.559638252769247 2307 60-61 0.4680593754406246 2307 62-63 0.5608082188041905 2307 64-65 0.522516339709135 2307 66-67 0.3895316078961457 2307 68-69 0.48155073859977193 2307 70-71 0.5091368477212015 2307 72-73 0.5010633955959847 2307 74-75 0.4608065875197056 2307 76-77 0.42864864746244535 2307 78-79 0.41343115458031576 2307 80-81 0.3562601116339721 2307 82-83 0.33591259577738697 2307 84-85 0.3511489803348766 2307 86-87 0.40451521938252455 2307 88-89 0.3356142073803383 2307 90-91 0.2549768709813307 2307 92-93 0.19149482838815146 2307 94-95 0.2638589789723156 2307 96-97 0.18040744662666697 2307 98-99 0.08991558403658217 2307 100-101 0.155957680114998 2201 1 0.2363040073690854 2201 2 0.24170035232998544 2201 3 0.2564550752423642 2201 4 0.40353479472241816 2201 5 0.34766538090324417 2201 6 0.3625560191650834 2201 7 0.3769233263718945 2201 8 0.3456118612828689 2201 9 0.3989046898065354 2201 10-11 0.3587656363417224 2201 12-13 0.40253327834604846 2201 14-15 0.5314951440025055 2201 16-17 0.4931618316887523 2201 18-19 0.4510482018203561 2201 20-21 0.42659511024130126 2201 22-23 0.44065555234828224 2201 24-25 0.54335227987103 2201 26-27 0.5083450140853216 2201 28-29 0.4601531900082989 2201 30-31 0.48610052167452267 2201 32-33 0.3898090638054086 2201 34-35 0.41004515574702083 2201 36-37 0.4976392219472814 2201 38-39 0.4859138597877646 2201 40-41 0.579195985528969 2201 42-43 0.633745131492681 2201 44-45 0.5507044140862831 2201 46-47 0.5530575700755733 2201 48-49 0.5215642139993903 2201 50-51 0.5047844900890723 2201 52-53 0.5368317385675496 2201 54-55 0.569764951063874 2201 56-57 0.5099891665709464 2201 58-59 0.508487517367918 2201 60-61 0.4909892008798096 2201 62-63 0.5043048258664697 2201 64-65 0.44706256098769614 2201 66-67 0.5030900341129154 2201 68-69 0.5649597263285244 2201 70-71 0.5265822328483836 2201 72-73 0.5035262741871733 2201 74-75 0.502988799713286 2201 76-77 0.4687669745274903 2201 78-79 0.49106194347965015 2201 80-81 0.4800916505537671 2201 82-83 0.4190262729746337 2201 84-85 0.44174041247207896 2201 86-87 0.5404387602216545 2201 88-89 0.4879513292052593 2201 90-91 0.35590544269513913 2201 92-93 0.3282265155529771 2201 94-95 0.288058536509034 2201 96-97 0.3817369304281186 2201 98-99 0.3665804908031518 2201 100-101 0.2718820972967926 1101 1 0.25163197328680553 1101 2 0.26367439108042845 1101 3 0.2905375764905358 1101 4 0.3778905974282054 1101 5 0.3732132039286 1101 6 0.29940995001994963 1101 7 0.3790657178710788 1101 8 0.34513624280248223 1101 9 0.3630107819878532 1101 10-11 0.40708847394908076 1101 12-13 0.40937894580078904 1101 14-15 0.5424302245249635 1101 16-17 0.5272076735182338 1101 18-19 0.4980733537248696 1101 20-21 0.5036507392457352 1101 22-23 0.4438580046894387 1101 24-25 0.49125850038035423 1101 26-27 0.5421040899569292 1101 28-29 0.4702823485235328 1101 30-31 0.473895027555578 1101 32-33 0.4174289817101098 1101 34-35 0.4818379335930345 1101 36-37 0.527989997991817 1101 38-39 0.5710277629183977 1101 40-41 0.5416492614043662 1101 42-43 0.5873910080816742 1101 44-45 0.5844795069092399 1101 46-47 0.5601326695361948 1101 48-49 0.5162890575254835 1101 50-51 0.5715779268907326 1101 52-53 0.5537365084124488 1101 54-55 0.6220941829460429 1101 56-57 0.5948174020031374 1101 58-59 0.5118525040877984 1101 60-61 0.5644665563737234 1101 62-63 0.5405378874193403 1101 64-65 0.49814264702710176 1101 66-67 0.5180259053821601 1101 68-69 0.5380614610653609 1101 70-71 0.5160264045539478 1101 72-73 0.6037456695448569 1101 74-75 0.5461466194326334 1101 76-77 0.5649488555877511 1101 78-79 0.6010766425197005 1101 80-81 0.5286200149873537 1101 82-83 0.4825049784458244 1101 84-85 0.4821815792757249 1101 86-87 0.5943800204975886 1101 88-89 0.49255556941655954 1101 90-91 0.5042343006881111 1101 92-93 0.4451031234608962 1101 94-95 0.449121439999125 1101 96-97 0.49833677696040013 1101 98-99 0.5414848219856125 1101 100-101 0.5501346607304889 2304 1 0.06719468426941688 2304 2 -2.470790455006977E-4 2304 3 0.04369008353300785 2304 4 0.060676552107807424 2304 5 0.14034649747427608 2304 6 -0.0033001830263401644 2304 7 0.02850937508593887 2304 8 0.07236095635529693 2304 9 0.09845005020694231 2304 10-11 0.052650910904077364 2304 12-13 0.04107550725681364 2304 14-15 0.15323435971934174 2304 16-17 0.18776042960390527 2304 18-19 0.02819686322863646 2304 20-21 0.16291414439759677 2304 22-23 0.1313657455767796 2304 24-25 0.061129745878233166 2304 26-27 0.10853326210382619 2304 28-29 0.12701393094345548 2304 30-31 0.13682827874868764 2304 32-33 0.20756337537063274 2304 34-35 0.23381764565674956 2304 36-37 0.10488468494197178 2304 38-39 0.21807902563212878 2304 40-41 0.265721891911582 2304 42-43 0.2864279580743059 2304 44-45 0.34960689480662666 2304 46-47 0.03164941548896394 2304 48-49 0.05355540678599624 2304 50-51 0.054037482496475775 2304 52-53 0.18650979029664683 2304 54-55 0.14752585691003617 2304 56-57 -0.045757563666853684 2304 58-59 -0.045445773707577075 2304 60-61 0.1075744715080802 2304 62-63 -0.007129411342909009 2304 64-65 0.06983315710554194 2304 66-67 0.1713779893228633 2304 68-69 0.09812263596226956 2304 70-71 0.19593095108026404 2304 72-73 0.2651337073210911 2304 74-75 0.3302222234142178 2304 76-77 0.31530633206201486 2304 78-79 0.23722800956020507 2304 80-81 -0.0647869828297658 2304 82-83 -0.08485905969411078 2304 84-85 0.08058485809065274 2304 86-87 0.21768999448224235 2304 88-89 0.2141481754764314 2304 90-91 0.17224446687682615 2304 92-93 0.06293719354113847 2304 94-95 0.11347045600838612 2304 96-97 0.04870762896561587 2304 98-99 0.12440494026817106 2304 100-101 0.22694748095118555 2202 1 0.15108300738301494 2202 2 0.19826789367461117 2202 3 0.11692098381684701 2202 4 0.22223343520784766 2202 5 0.20460573029244244 2202 6 0.2169853381421163 2202 7 0.20487451906328147 2202 8 0.23757633389777766 2202 9 0.2061202077885369 2202 10-11 0.26870401264563526 2202 12-13 0.24976538693525896 2202 14-15 0.29195353445579997 2202 16-17 0.2816041157453384 2202 18-19 0.37403833431388733 2202 20-21 0.2966437474900445 2202 22-23 0.3167580893099924 2202 24-25 0.3021823139819588 2202 26-27 0.2915712095958085 2202 28-29 0.32660505786510186 2202 30-31 0.32931004936380504 2202 32-33 0.3207994951958639 2202 34-35 0.3890619944475091 2202 36-37 0.3094689218004376 2202 38-39 0.4177006274028443 2202 40-41 0.4127825938752352 2202 42-43 0.45174573388785433 2202 44-45 0.3617605794782577 2202 46-47 0.35420506133637275 2202 48-49 0.27717026470181594 2202 50-51 0.3581457583928085 2202 52-53 0.3238484790644449 2202 54-55 0.44526532922331796 2202 56-57 0.42054512083782214 2202 58-59 0.4175644849647071 2202 60-61 0.447549966303022 2202 62-63 0.42776120478507806 2202 64-65 0.44558056454786055 2202 66-67 0.3846766709495597 2202 68-69 0.3502427370260932 2202 70-71 0.37677768824104163 2202 72-73 0.3779832156017946 2202 74-75 0.330938440245923 2202 76-77 0.41162232795608134 2202 78-79 0.31545073488281616 2202 80-81 0.29635738857673033 2202 82-83 0.34112897249541874 2202 84-85 0.3994067561426604 2202 86-87 0.3343577142765568 2202 88-89 0.3935881615004533 2202 90-91 0.3924342380604813 2202 92-93 0.2600721548945728 2202 94-95 0.2675625560801649 2202 96-97 0.24610750311198615 2202 98-99 0.2622038270386824 2202 100-101 0.32951084405829434 1102 1 0.27609973330204696 1102 2 0.27835531516245027 1102 3 0.26663734764507296 1102 4 0.31960894283018604 1102 5 0.3411021552614457 1102 6 0.2870280293770051 1102 7 0.3733903539512653 1102 8 0.3044658012403261 1102 9 0.33998755181935536 1102 10-11 0.34962465051440716 1102 12-13 0.3643064864854111 1102 14-15 0.5199820498444936 1102 16-17 0.48738172679667713 1102 18-19 0.4842255879599833 1102 20-21 0.4745864737345258 1102 22-23 0.4561512698022838 1102 24-25 0.5100066035253974 1102 26-27 0.5240355559073677 1102 28-29 0.5538092876282477 1102 30-31 0.35431394887036305 1102 32-33 0.38441877483531783 1102 34-35 0.47936337288222575 1102 36-37 0.5253447235987778 1102 38-39 0.45629065667981905 1102 40-41 0.5408800967829848 1102 42-43 0.5806465599536423 1102 44-45 0.5682750933330141 1102 46-47 0.5621615286258574 1102 48-49 0.518806992950033 1102 50-51 0.3992424385589999 1102 52-53 0.547281996767687 1102 54-55 0.5206587875955506 1102 56-57 0.5706111827646083 1102 58-59 0.5512306603086614 1102 60-61 0.5204354949087033 1102 62-63 0.5839699583180007 1102 64-65 0.6058174908159017 1102 66-67 0.5851753672389179 1102 68-69 0.640750928316649 1102 70-71 0.6972719172707471 1102 72-73 0.6529377692630831 1102 74-75 0.6961013821346071 1102 76-77 0.7205800484084861 1102 78-79 0.7072897319090288 1102 80-81 0.6571469292866325 1102 82-83 0.6100369898723415 1102 84-85 0.6441477345527602 1102 86-87 0.7331127216373261 1102 88-89 0.7176815876039129 1102 90-91 0.6288917483749472 1102 92-93 0.6715515938918912 1102 94-95 0.7401546024417982 1102 96-97 0.7272682114905962 1102 98-99 0.6295921865655032 1102 100-101 0.70147785815929 2305 1 0.13341791764507605 2305 2 0.1409566196328953 2305 3 0.15966282670113685 2305 4 0.2282685599743246 2305 5 0.17682134221153234 2305 6 0.15407591792391884 2305 7 0.24874236993680654 2305 8 0.24382283421701345 2305 9 0.2162182829751842 2305 10-11 0.2424616462666549 2305 12-13 0.23285519512692332 2305 14-15 0.2936100623179101 2305 16-17 0.34773898344301557 2305 18-19 0.3002199091901545 2305 20-21 0.33630380506708235 2305 22-23 0.3207854710189295 2305 24-25 0.25204323441755605 2305 26-27 0.28497333173432793 2305 28-29 0.3122779367080355 2305 30-31 0.34240708346460735 2305 32-33 0.31866974850706953 2305 34-35 0.3298528418654101 2305 36-37 0.3003070195208224 2305 38-39 0.36495866349638817 2305 40-41 0.33411568850955575 2305 42-43 0.39915184503520607 2305 44-45 0.3603444134547402 2305 46-47 0.38227613913326053 2305 48-49 0.3917605721837347 2305 50-51 0.31421272527317257 2305 52-53 0.36193617084399676 2305 54-55 0.4071092894347714 2305 56-57 0.3780890304856257 2305 58-59 0.42478570818006034 2305 60-61 0.5019665376032307 2305 62-63 0.4784511197123962 2305 64-65 0.40402167648775134 2305 66-67 0.41208350675405825 2305 68-69 0.4306912289570164 2305 70-71 0.413286025930681 2305 72-73 0.4224774152452895 2305 74-75 0.4353444663825812 2305 76-77 0.44472842248502076 2305 78-79 0.3971934039648133 2305 80-81 0.3840435597864982 2305 82-83 0.417133375341038 2305 84-85 0.40129245129252666 2305 86-87 0.4349234486494211 2305 88-89 0.4420853686924673 2305 90-91 0.49251580995547783 2305 92-93 0.5078447934937387 2305 94-95 0.4584105790944548 2305 96-97 0.47742780481729596 2305 98-99 0.45671061593970563 2305 100-101 0.47975313929984864 2203 1 0.21157750774101203 2203 2 0.19373626067428518 2203 3 0.12123947509737576 2203 4 0.2156071787074083 2203 5 0.1875177291518284 2203 6 0.2510298475991277 2203 7 0.27090622150818433 2203 8 0.22656528660627373 2203 9 0.2772198968749251 2203 10-11 0.24516569452902104 2203 12-13 0.209409731634004 2203 14-15 0.3583892951826755 2203 16-17 0.3631571590049347 2203 18-19 0.27098148946060263 2203 20-21 0.32719993386699997 2203 22-23 0.30796830698277944 2203 24-25 0.3132887617141904 2203 26-27 0.30377846779436624 2203 28-29 0.28291116075312317 2203 30-31 0.3196833821245164 2203 32-33 0.28259666887014134 2203 34-35 0.32569455988501517 2203 36-37 0.33751313757301915 2203 38-39 0.33610423279480983 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-1.499060000959659 1213 66-67 -1.5108004771823431 1213 68-69 -1.3861168282895378 1213 70-71 -1.3484661145017185 1213 72-73 -1.70641906808617 1213 74-75 -1.4314093242450596 1213 76-77 -1.4518130539381673 1213 78-79 -1.4632618837495457 1213 80-81 -1.4560389972798689 1213 82-83 -1.4360323795841694 1213 84-85 -1.4524640595797749 1213 86-87 -1.5129544888163693 1213 88-89 -1.5267853173751362 1213 90-91 -1.4259405291291394 1213 92-93 -1.3966627715082467 1213 94-95 -1.4069741104656792 1213 96-97 -1.4382403047139363 1213 98-99 -1.4428724686918386 1213 100-101 -2.1902340970070178 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3315.0 3 996.0 4 955.0 5 1336.0 6 1231.0 7 2960.0 8 5725.0 9 8852.0 10 12738.0 11 17332.0 12 22451.0 13 27089.0 14 30217.0 15 32562.0 16 35029.0 17 37755.0 18 40569.0 19 43700.0 20 46831.0 21 51056.0 22 55769.0 23 60390.0 24 67492.0 25 75512.0 26 85526.0 27 98049.0 28 115575.0 29 138583.0 30 171455.0 31 217418.0 32 289048.0 33 398864.0 34 576396.0 35 889946.0 36 1680999.0 37 4059826.0 38 2217360.0 39 1418.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 22.692690016396366 19.697348967152863 13.69248962401293 43.91747139243784 2 24.53967957264419 29.492169010438452 30.979062167698878 14.989089249218484 3 19.21712542963311 31.608286476001645 26.339388212777898 22.835199881587346 4 21.10373468908113 36.68410576643471 19.907294774887937 22.304864769596225 5 23.689901928230118 39.30758125266705 19.40068858352884 17.601828235573993 6 17.263110142802972 41.654851423187715 21.842506556093603 19.239531877915702 7 16.759284195532732 18.739827626702226 40.78100580707773 23.71988237068731 8 18.368032629634566 24.649536935144052 27.445405740151195 29.53702469507018 9 20.167228880517555 25.331152840746235 26.13381323915681 28.3678050395794 10-11 20.827097951075345 34.903065526571034 21.171040517199668 23.09879600515395 12-13 19.52424167368467 28.222632020059613 27.60327549144922 24.649850814806502 14-15 19.964633540140007 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25.121563635709016 23.04709837746042 40-41 21.331157680880935 29.797221921459965 25.05597408167233 23.815646315986776 42-43 22.157440014303734 29.99826327816001 24.698863069451953 23.145433638084306 44-45 21.321209324740003 30.456900584762593 25.061156814022628 23.160733276474776 46-47 21.621087360638022 30.138750859828452 24.96485319306652 23.275308586467002 48-49 20.737692213042617 30.08809554049246 25.3719644279225 23.802247818542423 50-51 21.02793804073134 30.265755687418245 25.399429892861136 23.30687637898928 52-53 21.269275707651914 30.030342537254967 24.947378545107924 23.7530032099852 54-55 20.734682024806613 30.457584427602097 25.279122417600234 23.528611129991056 56-57 21.045495988726305 30.49723151544429 25.459820138197465 22.997452357631946 58-59 21.405610911271964 30.188230587853848 25.12782905143302 23.278329449441166 60-61 21.002327044610276 30.335745141839976 25.541537065770235 23.120390747779513 62-63 21.492093597415117 30.39892618666256 25.167385939641377 22.941594276280945 64-65 21.806811630876222 29.740219111747646 25.178017417520344 23.27495183985579 66-67 21.23626108027429 29.99012798549382 25.478194282141608 23.295416652090285 68-69 21.192934369968626 30.10806797088932 25.451521350827228 23.24747630831483 70-71 20.9377384274898 29.909654063025233 25.126979299566194 24.02562820991877 72-73 20.487699582564026 29.884936956424536 25.86712538991663 23.76023807109481 74-75 21.221908243184096 29.994745091257347 25.579264613499674 23.204082052058883 76-77 20.797874439627265 30.00679349348078 25.443670961227628 23.75166110566433 78-79 20.220343677306598 29.90498240032686 26.00420365673936 23.87047026562718 80-81 21.148661248691372 30.278876207143323 25.471931907904498 23.100530636260803 82-83 21.273335372652575 30.208085928064172 25.26916365416321 23.249415045120045 84-85 20.7332798589268 30.41901263229489 25.68105359052042 23.166653918257893 86-87 21.35974327820839 30.663569141951047 25.335179039929596 22.641508539910966 88-89 21.180609506225995 29.905827706249987 25.88675158678689 23.02681120073713 90-91 20.391842709875927 30.35883603488839 26.282211033736225 22.96711022149946 92-93 20.92126331462728 30.218766270680796 25.77521843776045 23.08475197693147 94-95 21.126697397318008 30.467748993216247 25.39825563624205 23.00729797322369 96-97 20.021320518329627 30.759791972201374 26.02958580621027 23.18930170325873 98-99 20.831936733826947 30.673400789448515 25.240005445661005 23.254657031063534 100-101 20.244771803024424 30.944652082415303 25.34622415259792 23.464351961962354 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2143.0 1 1565.5 2 924.0 3 842.5 4 822.0 5 856.5 6 933.0 7 994.0 8 1025.0 9 1048.0 10 1094.5 11 1197.5 12 1315.0 13 1378.5 14 1491.0 15 1657.0 16 1876.5 17 2248.5 18 2761.5 19 3557.0 20 4796.0 21 6683.5 22 10040.5 23 14505.0 24 20103.5 25 28323.5 26 39888.0 27 55050.0 28 74529.5 29 98623.5 30 126476.5 31 161542.5 32 200234.5 33 234659.5 34 272278.5 35 303506.5 36 323065.5 37 341926.0 38 355290.0 39 366999.5 40 384760.0 41 411167.0 42 434187.5 43 441551.0 44 442902.5 45 460645.5 46 500882.5 47 536688.0 48 548470.0 49 554250.0 50 560034.0 51 557803.0 52 566723.5 53 534946.0 54 462199.5 55 408194.5 56 305142.0 57 182885.0 58 110317.5 59 68489.0 60 42267.0 61 20262.0 62 9173.5 63 4415.0 64 2109.0 65 1195.5 66 796.0 67 553.5 68 415.0 69 338.0 70 270.5 71 203.0 72 169.0 73 142.5 74 102.5 75 90.0 76 81.5 77 63.0 78 46.0 79 40.0 80 37.0 81 26.0 82 22.0 83 16.0 84 11.5 85 16.0 86 11.5 87 5.5 88 5.0 89 5.0 90 5.5 91 4.5 92 2.0 93 1.5 94 1.5 95 1.0 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04402733532232148 2 0.022310510160402502 3 0.016735033652905077 4 0.019514167776240987 5 0.03175784535366202 6 0.0453437672754806 7 0.06481491439965754 8 0.07600028393630362 9 0.06731871634978372 10-11 0.06159696962526861 12-13 0.07519579774270639 14-15 0.08283626554927694 16-17 0.0702441206901373 18-19 0.06810599428255534 20-21 0.07248549666267291 22-23 0.07790179675753345 24-25 0.08261255815854401 26-27 0.044745780211790674 28-29 0.04505552890665164 30-31 0.04690541694540464 32-33 0.07184448894691897 34-35 0.09552735790816382 36-37 0.10864865678769094 38-39 0.10808508624565222 40-41 0.11128582275921556 42-43 0.09782466072838267 44-45 0.06990425753883152 46-47 0.10713002776983091 48-49 0.10938861200319214 50-51 0.11425424775163316 52-53 0.11791530524228155 54-55 0.11949416317303121 56-57 0.11492106785862552 58-59 0.11414239405626672 60-61 0.11709361078785871 62-63 0.13307148096443697 64-65 0.13539459617589422 66-67 0.14447195376140315 68-69 0.16250621110664173 70-71 0.14617126951793208 72-73 0.12937170488693098 74-75 0.14614545712669366 76-77 0.14715644245019824 78-79 0.13767899280049387 80-81 0.13096777107850624 82-83 0.10532746244834833 84-85 0.11213763167008323 86-87 0.13084731325272697 88-89 0.13028804477589467 90-91 0.11842725100184343 92-93 0.1454313143024309 94-95 0.14137876887799988 96-97 0.15496469079981845 98-99 0.1441622050665422 100-101 0.13524402389367016 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.1622325E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.56744833086294 #Duplication Level Percentage of deduplicated Percentage of total 1 73.89225817319982 22.586977837600703 2 11.34495691815331 6.935727688230347 3 4.227056512015545 3.876309945680187 4 2.2801005859717045 2.7878742740344156 5 1.5619464813659045 2.3872359182362732 6 1.0830106935605193 1.9862924050309927 7 0.8266575290820177 1.7688167915273383 8 0.5873378681349856 1.4362735949580292 9 0.4345340538437791 1.1954337514983118 >10 3.1349672983631423 18.5334489468075 >50 0.34229440772445635 7.2629730796397505 >100 0.24914332864742397 15.00865876354775 >500 0.021973091132232603 4.626735364894549 >1k 0.0127862684463026 7.050145848032156 >5k 8.057043702545061E-4 1.6661649128877192 >10k+ 1.7108598843895354E-4 0.8909308773941542 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGGAACGATCAGCTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCTA 24722 0.21271131206535698 No Hit CCTGGAACGATCAGCTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCT 24695 0.21247900054421126 No Hit CTGAAGAAGACGTTCAGACACTCGTTATCGATAACGGCTCTGGTATGTGC 18224 0.15680167264295225 No Hit CGTGGAGCGATCAACTTGTCCAACTCAAGACAGAATCAACAGGCTGCGCT 12712 0.10937570580757293 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.2695695568657734E-4 0.0 0.0 2 4.474147814658427E-4 0.0 0.0 3 0.0014110773876999654 0.0 0.0 4 0.004474147814658427 0.0 0.0 5 0.00698655389519739 0.0 0.0 6 0.011744638013478371 0.0 0.0 7 0.01238994779443872 0.0 0.0 8 0.014928166266216097 1.7208260825609332E-5 0.0 9 0.016382264305980086 1.7208260825609332E-5 0.0 10-11 0.020069134187866884 1.7208260825609332E-5 0.0 12-13 0.0256274024345387 2.1510326032011666E-5 0.0 14-15 0.031116837637908075 3.4416521651218665E-5 0.0 16-17 0.03637396132013173 3.4416521651218665E-5 0.0 18-19 0.04253021663049347 3.4416521651218665E-5 0.0 20-21 0.04703447890159671 3.4416521651218665E-5 0.0 22-23 0.05060519302291065 3.4416521651218665E-5 0.0 24-25 0.0573379250709303 3.4416521651218665E-5 0.0 26-27 0.06048273473681041 3.4416521651218665E-5 0.0 28-29 0.0628617767959509 3.4416521651218665E-5 0.0 30-31 0.0645266760308286 3.4416521651218665E-5 0.0 32-33 0.06609262776595905 3.4416521651218665E-5 0.0 34-35 0.06768439189232792 3.4416521651218665E-5 0.0 36-37 0.06923743743183916 4.302065206402333E-5 0.0 38-39 0.07055386938499827 4.302065206402333E-5 0.0 40-41 0.0722703934023528 4.302065206402333E-5 0.0 42-43 0.07407726078904178 4.302065206402333E-5 0.0 44-45 0.0750581316561015 4.732271727042566E-5 0.0 46-47 0.07598737774068443 5.1624782476828E-5 0.0 48-49 0.07766518317118132 5.1624782476828E-5 0.0 50-51 0.07914939566739013 5.1624782476828E-5 0.0 52-53 0.08010445414321145 5.1624782476828E-5 0.0 54-55 0.08117136631439922 5.1624782476828E-5 0.0 56-57 0.08207910207295012 5.1624782476828E-5 0.0 58-59 0.08299544196191382 5.1624782476828E-5 0.0 60-61 0.08413548924161043 5.1624782476828E-5 0.0 62-63 0.08534867162981588 5.1624782476828E-5 0.0 64-65 0.08643279206182927 5.1624782476828E-5 0.0 66-67 0.08769329716730517 5.1624782476828E-5 8.604130412804666E-6 68-69 0.08912588488103715 5.1624782476828E-5 8.604130412804666E-6 70-71 0.09079508618112125 5.1624782476828E-5 8.604130412804666E-6 72-73 0.09263637008946145 5.1624782476828E-5 8.604130412804666E-6 74-75 0.09465834073647054 5.1624782476828E-5 8.604130412804666E-6 76-77 0.09684378986132293 5.1624782476828E-5 8.604130412804666E-6 78-79 0.09931747735500426 5.1624782476828E-5 8.604130412804666E-6 80-81 0.10197185158735451 5.1624782476828E-5 8.604130412804666E-6 82-83 0.10523281701380749 5.1624782476828E-5 8.604130412804666E-6 84-85 0.10916490461245922 5.1624782476828E-5 8.604130412804666E-6 86-87 0.11351859460133837 5.1624782476828E-5 8.604130412804666E-6 88-89 0.11877571828356202 5.1624782476828E-5 8.604130412804666E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGGAG 3370 0.0 51.284027 1 TGGAGCG 4120 0.0 40.909924 3 GGAGCGA 4195 0.0 40.065342 4 CGGGACG 60 8.316038E-5 39.56666 1 AACCTAC 2440 0.0 39.111607 5 ACCTACT 2300 0.0 38.602303 6 GTGGAGC 4660 0.0 36.88217 2 GAGCGAT 4655 0.0 35.59633 5 CCTACTA 2460 0.0 35.516243 7 CCTGACG 2460 0.0 35.512573 6 AGCGATC 4760 0.0 34.81111 6 GACGTAC 2815 0.0 32.389523 9 GTCTGAG 1995 0.0 30.939344 1 TGGAACG 10145 0.0 30.794374 3 CTGACCC 2225 0.0 30.087538 3 GGAACGA 10490 0.0 29.781597 4 CTGACGT 3015 0.0 29.608395 7 GCGATCA 5710 0.0 29.02242 7 TGACCTG 3090 0.0 28.425676 3 CCGATCC 2320 0.0 28.03691 3 >>END_MODULE