FastQCFastQC Report
Thu 11 Sep 2014
SDW_B7268_METR_2_GTGAAA_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_B7268_METR_2_GTGAAA_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11622325
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAACGATCAGCTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCTA247220.21271131206535698No Hit
CCTGGAACGATCAGCTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCT246950.21247900054421126No Hit
CTGAAGAAGACGTTCAGACACTCGTTATCGATAACGGCTCTGGTATGTGC182240.15680167264295225No Hit
CGTGGAGCGATCAACTTGTCCAACTCAAGACAGAATCAACAGGCTGCGCT127120.10937570580757293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGAG33700.051.2840271
TGGAGCG41200.040.9099243
GGAGCGA41950.040.0653424
CGGGACG608.316038E-539.566661
AACCTAC24400.039.1116075
ACCTACT23000.038.6023036
GTGGAGC46600.036.882172
GAGCGAT46550.035.596335
CCTACTA24600.035.5162437
CCTGACG24600.035.5125736
AGCGATC47600.034.811116
GACGTAC28150.032.3895239
GTCTGAG19950.030.9393441
TGGAACG101450.030.7943743
CTGACCC22250.030.0875383
GGAACGA104900.029.7815974
CTGACGT30150.029.6083957
GCGATCA57100.029.022427
TGACCTG30900.028.4256763
CCGATCC23200.028.036913
Produced by FastQC (version 0.11.2)