FastQCFastQC Report
Thu 11 Sep 2014
SDW_B7268_METR_2_GTGAAA_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_B7268_METR_2_GTGAAA_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11622325
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGCA2900.032.781321
CCCGCGT1102.7215447E-630.2482171
CGGGGCT3650.029.9522461
CGGGTCT5600.029.7080731
CCCTATG2800.029.68723766-67
CGGGCTA1452.3879693E-829.5031851
CGGGCCG1303.0225237E-729.2510221
CCCGGCC856.3848105E-427.9605351
CTCGGTT20650.027.3918461
CCGGGAT6100.027.2729821
CCGCTAT8900.026.7038841
CGGGCCC1109.439362E-525.9270441
GCCCGGA1901.1847078E-825.0173231
CGGGGGC1751.4786383E-724.44551
CCTCGGG3050.023.35964414-15
CCGGGTC1452.3242352E-522.9469221
CCGGGCA2704.0017767E-1122.8862151
CGAGCCC3500.022.39246662-63
CGGGGTG1954.200192E-721.9382691
CCGTCCA12850.021.808774
Produced by FastQC (version 0.11.2)