FastQCFastQC Report
Thu 11 Sep 2014
SDW_B7268_METR_1_GTGAAA_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_B7268_METR_1_GTGAAA_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19449101
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAAGAAGACGTTCAGACACTCGTTATCGATAACGGCTCTGGTATGTGC494350.2541762727233511No Hit
CCTGGAACGATCAGCTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCT403190.20730521169076144No Hit
CTGGAACGATCAGCTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCTA361090.1856589669620205No Hit
CGTTCAGACACTCGTTATCGATAACGGCTCTGGTATGTGCAAGGCTGGCT287450.14779603437711594No Hit
CTTACATCTTCAGTCAACTTCCCAGCACGTGAACTTTCACGCAATGTTCG264890.13619652651297354No Hit
CAGACACTCGTTATCGATAACGGCTCTGGTATGTGCAAGGCTGGCTTCTC242570.12472041766866243No Hit
AGAAGACGTTCAGACACTCGTTATCGATAACGGCTCTGGTATGTGCAAGG214970.11052953038806267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGAG51250.049.384691
GGAGCGA58000.042.893984
TGGAGCG59850.041.7266463
AACCTAC43350.041.0709955
CCTGACG45600.039.356826
GTGGAGC65200.039.099242
CCTACTA41700.038.486997
ACCTACT42350.038.34126
GAGCGAT67200.037.5868035
AGCGATC68950.036.288536
CTGACGT52000.035.0639727
TGACCTG52150.034.959793
GACGTAC51250.034.9316259
GTCTGAG31750.032.3049431
TGGAACG159300.031.5327473
CCGATCC44400.030.4757083
ACCTGAC58950.030.443955
GACCTGA61300.029.9739194
GGAACGA169300.029.5861574
TGACGTA62700.029.2359228