FastQCFastQC Report
Thu 11 Sep 2014
SDW_B7268_METR_1_GTGAAA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_B7268_METR_1_GTGAAA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19449101
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAACACGAAGTTCGTTGTAGAATGTGTGGTGCCAGATCTTTTCCATAT338340.17396176820717832No Hit
CTCTGTTGGGTCAACACGAAGTTCGTTGTAGAATGTGTGGTGCCAGATCT296290.1523412316075689No Hit
GCCAGATCTTTTCCATATCATCCCAGTTGTTGACGATACCGTGTTCAATT208860.10738799700819077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGACG2250.035.9088131
CGGGCCC1651.8189894E-1234.5646321
CTCGGTT35350.034.1490751
CCGCTAT17550.030.6010251
CCCGTGT3350.028.3739531
CGGGGTG2850.028.3490641
CCGGGCA3450.026.1739431
CGGGGCT8000.025.5454241
CCCGGAA5600.025.4605561
CGGGTCG1901.1865268E-825.0138781
CCCGCGT1601.8531264E-623.7631861
CCCGGTA2503.8198777E-1022.8126581
CCCGCTA11250.022.8126581
CCGGGTT9600.022.7730521
CGGGGGA4450.022.4281751
CGGGGGT4050.022.2963221
GGTTTTA70050.022.238274
CGGGTCT7300.022.1355691
CTCGGGT5800.022.1243441
CGGGGCA4300.022.105291
Produced by FastQC (version 0.11.2)