FastQCFastQC Report
Thu 11 Sep 2014
SDW_B7268_20A_3_AGTTCC_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_B7268_20A_3_AGTTCC_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16766816
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGAACGATCAGCTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCT378220.22557651971608683No Hit
CTGGAACGATCAGCTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCTA356130.2124016867603247No Hit
CTGAAGAAGACGTTCAGACACTCGTTATCGATAACGGCTCTGGTATGTGC241920.14428499722308638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGAG51800.050.50331
GGAGCGA58850.043.6456534
TGGAGCG62750.040.933623
GTGGAGC65900.039.335952
GAGCGAT66400.038.826055
AGCGATC68000.037.7015726
AACCTAC32850.035.2651635
ACCTACT30250.034.214156
GTCTGAG25750.033.5576931
CCTACTA31350.033.169947
CCTGACG19900.033.1616636
CGTACTG15550.031.1464047
GCGATCA82150.031.0965947
TGGAACG156050.029.9079323
GGAACGA159200.029.1069644
CTGACCC25850.026.448353
GACGTAC27250.025.6133049
AAGACGT202050.024.2761367
CTGGAAC189650.024.13361
CTGACGT28750.024.1131047
Produced by FastQC (version 0.11.2)