FastQCFastQC Report
Thu 11 Sep 2014
SDW_B7268_20A_3_AGTTCC_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_B7268_20A_3_AGTTCC_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16766816
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAACACGAAGTTCGTTGTAGAATGTGTGGTGCCAGATCTTTTCCATAT178470.10644239192462064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGCT5850.038.183241
CCGGGCT2200.036.724591
CTCGGTT25500.032.429471
CCGCTAT11650.031.004051
CGGGACG1859.094947E-1230.827641
CGGGGTG2800.028.855041
CGGGCTA2900.027.8600371
CCGGGAT9150.027.5286881
CGGGGAT8850.027.3878331
CGGGGGA4450.024.563971
CCGGGGA5450.024.4169981
CCGGGTT9200.024.279561
CGCGGGG1401.7738412E-523.7629741
CGGGGTA4000.023.7629721
CGGGGTT10000.023.2877141
CGGGGTC3500.023.084031
CATTACC67500.022.6585889
CGGGGCA3400.022.365151
CCCTATG3250.021.19180766-67
CCCGGTA2509.453288E-920.9114171
Produced by FastQC (version 0.11.2)