FastQCFastQC Report
Thu 11 Sep 2014
SDW_B7268_20A_2_AGTTCC_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_B7268_20A_2_AGTTCC_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15186986
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAACGATCAGCTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCTA362120.23844099151734255No Hit
CCTGGAACGATCAGCTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCT317250.20889595868462643No Hit
CTGAAGAAGACGTTCAGACACTCGTTATCGATAACGGCTCTGGTATGTGC264580.17421494956273745No Hit
CTTACATCTTCAGTCAACTTCCCAGCACGTGAACTTTCACGCAATGTTCG164720.10846128389135276No Hit
CGTTCAGACACTCGTTATCGATAACGGCTCTGGTATGTGCAAGGCTGGCT155800.10258783408373458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGAG44100.051.457631
TGGAGCG53450.042.013023
GGAGCGA54000.041.585114
GTGGAGC59250.038.3808672
AGCGATC61450.037.470576
AACCTAC29650.037.4680945
ACCTACT27700.036.8492976
GAGCGAT62250.036.226365
CCTACTA28800.034.786947
TGGAACG141350.030.6315863
GCGATCA74050.030.521667
CTGACCC29050.030.0706043
GACCCGC21400.029.0621685
GGAACGA151300.028.5543924
AGACCCG22800.027.9023254
CCGATCC31650.027.1503433
AAGACGT206900.025.9324917
ACGACGC69300.025.7620777
GTCTGAG21750.025.7562831
CTGGAAC173700.025.6914421
Produced by FastQC (version 0.11.2)