FastQCFastQC Report
Thu 11 Sep 2014
SDW_B7268_20A_2_AGTTCC_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_B7268_20A_2_AGTTCC_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15186986
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGATTTCATTACCTTCTTTCTTAACGAAGAAAAGAGCTGGAATTGAAG174880.1151512222372497No Hit
GCCAGATCTTTTCCATATCATCCCAGTTGTTGACGATACCGTGTTCAATT169550.11164163843964826No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGACG1600.041.59071
CGGGCCC1106.980554E-834.5688931
CGGGCTA2600.032.9069251
CGGGGCT4350.030.5954551
CGGGTCT6100.030.3894561
CGGGGGA3800.028.7695051
CTCGGTT24400.028.2466091
CCGGGCT3750.026.6180461
CGGGGCA3300.025.926671
CATTACC67350.025.6708439
CGGGTCG1551.4042162E-624.532761
CGGGGTT9550.024.3882641
GCGGGGT3200.023.7661151
CGGGGTG3050.023.3765051
CGGGGAT8750.023.3586941
TCATTAC76550.023.0818828
CCCTATG3200.023.00727866-67
CCGGCCG2354.623871E-922.2491261
CCGTGCG652.9448152E-421.9220642-43
CCTCGGG3800.021.8741314-15
Produced by FastQC (version 0.11.2)