FastQCFastQC Report
Thu 11 Sep 2014
SDW_B7268_20A_1_AGTTCC_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_B7268_20A_1_AGTTCC_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18596618
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGAACGATCAGCTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCT432030.23231643517116932No Hit
CTGAAGAAGACGTTCAGACACTCGTTATCGATAACGGCTCTGGTATGTGC352010.1892871058597859No Hit
CTGGAACGATCAGCTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCTA351390.1889537119061111No Hit
CTTACATCTTCAGTCAACTTCCCAGCACGTGAACTTTCACGCAATGTTCG234820.12627027129341475No Hit
CGTTCAGACACTCGTTATCGATAACGGCTCTGGTATGTGCAAGGCTGGCT201460.10833152565697698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGAG53250.053.1479641
GGAGCGA63150.044.5813944
GTGGAGC65500.043.1215362
TGGAGCG67550.041.5369343
GAGCGAT70550.039.9728625
AGCGATC72200.039.2566266
AACCTAC38400.035.3598675
GTCTGAG28300.034.5653341
TGGAACG163500.034.3219533
CCTGACG38000.034.107896
ACCTACT36600.033.7262546
GGAACGA172150.032.376764
CCTACTA37200.032.0381477
GACGTAC41350.031.9264289
GCGATCA91800.030.8276337
TGACCTG44350.029.8664043
CTGACGT43650.029.3708237
ACCTGAC46000.027.9696675
GACCTGA47100.027.6186184
TGACGTA49700.026.4677668
Produced by FastQC (version 0.11.2)