FastQCFastQC Report
Thu 11 Sep 2014
SDW_B7268_20A_1_AGTTCC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_B7268_20A_1_AGTTCC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18596618
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAACACGAAGTTCGTTGTAGAATGTGTGGTGCCAGATCTTTTCCATAT231530.12450113241020491No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTT32650.034.9370041
CGGGGCA4300.033.1596831
CCGGGGA5550.032.5422941
CGGGGGT4200.030.554281
CGGGGCT6500.029.9797531
CGGGCTA2550.029.8220421
CGGGGAT9500.029.0176321
CGGGACG1354.2085048E-728.1652621
CCCGCTA8850.027.9265731
CGGGTCG2059.531504E-1025.50331
CCGCTAT14750.025.1339151
CCTCGGG4200.024.87753914-15
CGGGGTA5100.024.230411
CGGGGTC6200.023.764441
CGGGGGA4600.023.764441
CATTACC79100.023.6566129
CGGGGTT11850.023.263081
CCCGTGT3100.022.9978451
CGGGTCT6550.022.4945851
CCGGGTA4450.022.429361
Produced by FastQC (version 0.11.2)