##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename NYCLib9_ACTTGA_L007_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1035550 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.858817053739557 27.0 22.0 33.0 2.0 33.0 2 24.886507652938054 33.0 15.0 33.0 2.0 33.0 3 25.774648254550723 33.0 22.0 33.0 2.0 33.0 4 27.903266863019653 33.0 22.0 37.0 2.0 37.0 5 27.97876201052581 33.0 15.0 37.0 6.0 37.0 6 29.038438510936217 33.0 27.0 37.0 15.0 37.0 7 28.695020037661145 33.0 27.0 37.0 6.0 37.0 8 28.455626478682827 33.0 27.0 37.0 2.0 37.0 9 29.225402925981363 33.0 27.0 37.0 15.0 37.0 10-11 28.846835015209308 33.0 24.5 37.0 10.5 37.0 12-13 29.155570469798658 33.0 27.0 37.0 10.5 37.0 14-15 29.48568103906137 33.0 27.0 37.0 6.0 40.0 16-17 30.38268456375839 33.0 27.0 37.0 15.0 40.0 18-19 30.691758968663997 33.0 27.0 37.0 15.0 40.0 20-21 30.711257302882526 33.0 27.0 37.0 15.0 40.0 22-23 30.740701076722516 33.0 27.0 37.0 15.0 40.0 24-25 30.32029163246584 33.0 27.0 37.0 10.5 40.0 26-27 29.80937955675728 33.0 27.0 37.0 10.5 40.0 28-29 30.022625175027763 33.0 27.0 37.0 15.0 40.0 30-31 29.93006132007146 33.0 27.0 37.0 15.0 40.0 32-33 29.754402491429673 33.0 27.0 37.0 15.0 40.0 34-35 29.78818743662788 33.0 27.0 37.0 15.0 40.0 36-37 30.023548838781323 33.0 27.0 37.0 15.0 40.0 38-39 30.071363043793156 33.0 27.0 37.0 15.0 40.0 40-41 30.160089807348754 33.0 27.0 37.0 15.0 40.0 42-43 30.077037323161605 33.0 27.0 37.0 15.0 40.0 44-45 29.830702042392932 33.0 27.0 37.0 15.0 40.0 46-47 29.449607937810825 33.0 27.0 37.0 15.0 40.0 48-49 29.301255371541693 33.0 24.5 37.0 10.5 40.0 50-51 28.52719376176911 33.0 22.0 37.0 10.5 38.5 52-53 28.23987542851625 33.0 22.0 37.0 10.5 37.0 54-55 29.190977258461686 33.0 24.5 37.0 15.0 40.0 56-57 29.112761334556517 33.0 22.0 37.0 10.5 40.0 58-59 29.30809666360871 33.0 27.0 37.0 15.0 40.0 60-61 29.072531504997343 33.0 24.5 37.0 10.5 40.0 62-63 28.967352614552652 33.0 22.0 37.0 15.0 40.0 64-65 28.926315484525134 33.0 24.5 37.0 15.0 40.0 66-67 28.812482255806096 33.0 22.0 37.0 15.0 40.0 68-69 28.529605523634785 33.0 22.0 37.0 15.0 40.0 70-71 28.421919752788373 33.0 22.0 37.0 15.0 37.0 72-73 28.18529235671865 33.0 22.0 37.0 15.0 37.0 74-75 28.143282796581524 33.0 22.0 37.0 15.0 37.0 76-77 27.737092849210566 33.0 22.0 37.0 6.0 37.0 78-79 27.15023079522959 27.0 22.0 35.0 6.0 37.0 80-81 27.021394910916904 27.0 22.0 33.0 6.0 37.0 82-83 26.58560475109845 27.0 22.0 33.0 6.0 37.0 84-85 26.679422046255613 27.0 22.0 33.0 6.0 37.0 86-87 26.37333397711361 27.0 22.0 33.0 6.0 37.0 88-89 25.996640432620346 27.0 22.0 33.0 6.0 37.0 90-91 25.85646178359326 27.0 22.0 33.0 6.0 37.0 92-93 25.732504466225677 27.0 22.0 33.0 6.0 37.0 94-95 25.490429723335424 27.0 22.0 33.0 6.0 37.0 96-97 25.339301337453527 27.0 22.0 33.0 6.0 37.0 98-99 25.092573028825264 27.0 15.0 33.0 6.0 37.0 100-101 24.579073439235188 27.0 15.0 33.0 6.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 8.21338785166109 2306 2 8.484234516682914 2306 3 7.436799240721992 2306 4 9.072990541537912 2306 5 8.77739826993659 2306 6 7.848674551552527 2306 7 7.977996582467377 2306 8 8.457549011363255 2306 9 7.3006108491282475 2306 10-11 7.690604153354236 2306 12-13 7.352839099449309 2306 14-15 8.062192302134829 2306 16-17 6.7447932586205965 2306 18-19 6.077860192283744 2306 20-21 6.093896406226207 2306 22-23 5.710058742597887 2306 24-25 5.597193699860021 2306 26-27 6.257458104767885 2306 28-29 5.465209987655221 2306 30-31 5.478841909143547 2306 32-33 5.391380721240683 2306 34-35 4.831469725735214 2306 36-37 4.741478183953358 2306 38-39 4.465607788986656 2306 40-41 3.710444024735331 2306 42-43 3.642950011842643 2306 44-45 3.145820462335461 2306 46-47 3.4325955442137825 2306 48-49 3.2165788848229937 2306 50-51 3.2761205981410733 2306 52-53 3.811446689010978 2306 54-55 3.347277013497596 2306 56-57 3.736618134754085 2306 58-59 3.2891445380259015 2306 60-61 3.4570282062029776 2306 62-63 2.884601074116489 2306 64-65 2.460138618663695 2306 66-67 2.2902756552716994 2306 68-69 2.5142553044986364 2306 70-71 2.812591273275249 2306 72-73 1.702369171968659 2306 74-75 1.4769359971675051 2306 76-77 1.9734637593260942 2306 78-79 2.1378227795549236 2306 80-81 1.7220846406248214 2306 82-83 1.7093796533797132 2306 84-85 1.3631661593247983 2306 86-87 1.3086982569333188 2306 88-89 1.1944127253195695 2306 90-91 1.2431944091272662 2306 92-93 1.0044779040500806 2306 94-95 1.2221174382679258 2306 96-97 1.0241891436622481 2306 98-99 0.9971851010148995 2306 100-101 0.7438640872819207 2307 1 8.341587210173234 2307 2 8.578289979689622 2307 3 7.606211293068725 2307 4 9.098039233283941 2307 5 9.072554373602358 2307 6 7.638095803531272 2307 7 8.165110467281352 2307 8 8.666524454147343 2307 9 7.548004286724073 2307 10-11 7.793962019039604 2307 12-13 7.368325146674078 2307 14-15 8.167577194847222 2307 16-17 6.845223403576966 2307 18-19 6.5623629563173225 2307 20-21 6.427597932039049 2307 22-23 6.179857428127939 2307 24-25 6.2327929605742725 2307 26-27 7.090672920319406 2307 28-29 6.158512489789366 2307 30-31 6.0294877481748514 2307 32-33 6.204688693426295 2307 34-35 5.8828892455310005 2307 36-37 5.967052539357521 2307 38-39 5.5591483626790215 2307 40-41 4.98344041118618 2307 42-43 4.790181212684708 2307 44-45 4.793146560282533 2307 46-47 4.989642379457486 2307 48-49 4.764978687569627 2307 50-51 4.561350423182308 2307 52-53 5.142794849498969 2307 54-55 4.878218442507098 2307 56-57 5.173764342599462 2307 58-59 4.5971069852937845 2307 60-61 4.693855949309473 2307 62-63 4.471464869834932 2307 64-65 4.379831632744931 2307 66-67 4.015840272326653 2307 68-69 4.120972486261817 2307 70-71 3.8048950766186103 2307 72-73 3.1811126348427585 2307 74-75 2.913446311529704 2307 76-77 3.0316410718732314 2307 78-79 3.5057498372473894 2307 80-81 3.2057372674927223 2307 82-83 3.277236885036391 2307 84-85 2.51872178394666 2307 86-87 2.4529358477900445 2307 88-89 2.6385475455123455 2307 90-91 2.4027761159490844 2307 92-93 2.213479453897996 2307 94-95 2.486152264262273 2307 96-97 2.349124083201602 2307 98-99 2.1792748264348916 2307 100-101 1.6114345556343572 1101 1 7.970526714662022 1101 2 7.908356914943244 1101 3 7.21262713758437 1101 4 8.658253045421105 1101 5 8.560967701437058 1101 6 7.274272563357435 1101 7 7.101028579982135 1101 8 8.052941990549336 1101 9 7.273369469817901 1101 10-11 7.414971969446192 1101 12-13 7.120195421288383 1101 14-15 7.664219329161849 1101 16-17 6.690965983069184 1101 18-19 5.933091630618627 1101 20-21 5.775870621821113 1101 22-23 5.207474088978749 1101 24-25 5.766525790571421 1101 26-27 6.454528623562538 1101 28-29 5.761814523225276 1101 30-31 5.56235697593447 1101 32-33 5.532334433573698 1101 34-35 5.4506138698793585 1101 36-37 5.266590019927261 1101 38-39 4.904034318964914 1101 40-41 4.354434394415364 1101 42-43 4.1936909251697685 1101 44-45 4.143625681347583 1101 46-47 4.322564478665477 1101 48-49 4.20626356950768 1101 50-51 3.645656168193888 1101 52-53 4.459245694913431 1101 54-55 4.1749781629228835 1101 56-57 4.454179489211988 1101 58-59 3.8797239105018306 1101 60-61 4.189415593590365 1101 62-63 3.8137203658219896 1101 64-65 3.656910908194604 1101 66-67 3.1820246456413805 1101 68-69 3.3155460780307884 1101 70-71 3.2107180207123385 1101 72-73 2.6020740492597447 1101 74-75 2.251031989711773 1101 76-77 2.5345230945233617 1101 78-79 3.0006544042831003 1101 80-81 2.374580757431282 1101 82-83 2.717475335268496 1101 84-85 2.221946836553748 1101 86-87 2.0048228297820287 1101 88-89 2.128222914819414 1101 90-91 2.0659468167072603 1101 92-93 1.7859783700333018 1101 94-95 2.0378148598274137 1101 96-97 1.7604348721493537 1101 98-99 1.735759192347615 1101 100-101 1.2794155007643653 2201 1 6.973345111825818 2201 2 7.817350412661881 2201 3 6.368879661391624 2201 4 8.160129963117171 2201 5 3.9409292282808295 2201 6 5.576582542531057 2201 7 6.5435754393325425 2201 8 7.584324957433807 2201 9 6.444110157565312 2201 10-11 3.985071649896419 2201 12-13 6.299358232690434 2201 14-15 7.115026152582782 2201 16-17 5.885817893213705 2201 18-19 1.813550446853668 2201 20-21 4.9280748793051 2201 22-23 4.9056152950009135 2201 24-25 5.216497651114182 2201 26-27 6.085402854496863 2201 28-29 4.932643603844021 2201 30-31 5.142464764363915 2201 32-33 5.304205786970304 2201 34-35 4.91700391137314 2201 36-37 4.975826121102127 2201 38-39 4.266785626575281 2201 40-41 3.958516682455297 2201 42-43 3.9339487907044983 2201 44-45 3.7924732354405215 2201 46-47 4.10235430712607 2201 48-49 3.8726443901623604 2201 50-51 3.575723416727296 2201 52-53 4.34154867896774 2201 54-55 4.092251656190083 2201 56-57 4.14148461949609 2201 58-59 3.7956044211455406 2201 60-61 3.8814026844156295 2201 62-63 3.5401469727121793 2201 64-65 3.5069698897688255 2201 66-67 3.252698243835365 2201 68-69 3.352651688434438 2201 70-71 3.183721947517956 2201 72-73 2.718016562097379 2201 74-75 2.363080128851056 2201 76-77 2.59280197721203 2201 78-79 2.979199737425656 2201 80-81 2.4092075519178593 2201 82-83 2.619748113125766 2201 84-85 2.2932388668871297 2201 86-87 2.2082281810483053 2201 88-89 2.36666204933238 2201 90-91 2.0224250920923907 2201 92-93 2.0016649250960192 2201 94-95 2.210988259296421 2201 96-97 1.915281376974395 2201 98-99 1.9437895893206303 2201 100-101 1.694056603449539 2304 1 1.7458442360042632 2304 2 4.940426471931474 2304 3 5.639575344441489 2304 4 4.334378516133437 2304 5 6.428146187665639 2304 6 6.515229957730568 2304 7 6.981402389646767 2304 8 6.487730736827999 2304 9 5.759873284805778 2304 10-11 6.52462359303405 2304 12-13 6.51121531442929 2304 14-15 6.749296516125835 2304 16-17 5.392223450390411 2304 18-19 5.294599487324923 2304 20-21 5.058205354969118 2304 22-23 4.962218277776294 2304 24-25 5.030228793272471 2304 26-27 5.6978108932338785 2304 28-29 5.159188786351002 2304 30-31 5.102676764582178 2304 32-33 5.06973669299288 2304 34-35 4.804873446780444 2304 36-37 4.886172835723482 2304 38-39 4.419665319930981 2304 40-41 3.8155940718665136 2304 42-43 3.0896149014320677 2304 44-45 3.1318329327818937 2304 46-47 4.225854240302667 2304 48-49 3.8491909565860105 2304 50-51 3.264253199009527 2304 52-53 4.0445957471600344 2304 54-55 3.7937321307074328 2304 56-57 4.5418311522312536 2304 58-59 3.828084550079126 2304 60-61 3.752491637043768 2304 62-63 3.591005119671479 2304 64-65 3.339720001075264 2304 66-67 3.0874925977716607 2304 68-69 3.365990272743037 2304 70-71 3.2127944781954376 2304 72-73 2.8665286144299884 2304 74-75 2.4641831499806948 2304 76-77 2.6568156360741995 2304 78-79 3.056402122190871 2304 80-81 2.9440442932448505 2304 82-83 3.1503544188827775 2304 84-85 2.632696624609977 2304 86-87 2.2670988101451925 2304 88-89 2.4457690988457514 2304 90-91 2.3150594130677433 2304 92-93 2.3142881431056033 2304 94-95 2.364304479511567 2304 96-97 2.123046019405912 2304 98-99 2.129397330396941 2304 100-101 1.816759796026684 1102 1 7.308090256022297 1102 2 7.642464221550853 1102 3 6.331370555639516 1102 4 6.119528198110693 1102 5 6.507518329785704 1102 6 5.860431014488114 1102 7 7.123683965786469 1102 8 6.553667633748809 1102 9 4.911708022568618 1102 10-11 6.069630746430001 1102 12-13 5.953357580800695 1102 14-15 6.405112455102643 1102 16-17 5.417370929115982 1102 18-19 5.621012802997818 1102 20-21 5.673081549723946 1102 22-23 5.2666667806409535 1102 24-25 1.7926164181126438 1102 26-27 5.462276545888386 1102 28-29 4.700947004149889 1102 30-31 4.756887026513802 1102 32-33 5.094334076000354 1102 34-35 5.086104754642701 1102 36-37 4.964334193500289 1102 38-39 3.9467656025955513 1102 40-41 4.091038831785227 1102 42-43 4.154362817021131 1102 44-45 -3.8803948272139976 1102 46-47 1.575137910965882 1102 48-49 -0.7449690780922573 1102 50-51 2.724138096321642 1102 52-53 -0.3059189330084351 1102 54-55 2.689115113514319 1102 56-57 -0.6756751168517638 1102 58-59 2.720429598801683 1102 60-61 3.899855466478808 1102 62-63 3.6789749638115055 1102 64-65 3.223693267140572 1102 66-67 3.231528599133771 1102 68-69 3.4798491848817754 1102 70-71 3.299954024887878 1102 72-73 2.8253751433188334 1102 74-75 2.5835206425253574 1102 76-77 2.946553130684407 1102 78-79 -1.005567487279901 1102 80-81 1.8675732426237097 1102 82-83 2.679207835801975 1102 84-85 2.441640707631258 1102 86-87 2.459153792750122 1102 88-89 2.537217383967178 1102 90-91 2.2689448961923553 1102 92-93 2.1750896849227743 1102 94-95 2.725604191453627 1102 96-97 2.6997137274532648 1102 98-99 2.5809392766657133 1102 100-101 1.9885551908290182 2202 1 -20.71499052671729 2202 2 -7.709120298390939 2202 3 -0.30993686691984834 2202 4 1.807073237912661 2202 5 1.138640380809015 2202 6 2.102585394003107 2202 7 4.284232948223153 2202 8 2.943520102602367 2202 9 3.4945418411183873 2202 10-11 2.2909738391213317 2202 12-13 1.9760676884819581 2202 14-15 3.0912680044545198 2202 16-17 1.9369973851130808 2202 18-19 1.8608771278884788 2202 20-21 1.2993180478308481 2202 22-23 1.7568772801742831 2202 24-25 2.4830906874205922 2202 26-27 3.6588977144329995 2202 28-29 -0.8086713798490699 2202 30-31 1.1127876386068323 2202 32-33 1.619139626171485 2202 34-35 1.766510357864572 2202 36-37 3.1354412320291623 2202 38-39 2.6629979240385317 2202 40-41 2.327886282149862 2202 42-43 1.0818465331328824 2202 44-45 0.9758695039833931 2202 46-47 -0.9069483434816767 2202 48-49 1.0374157568151325 2202 50-51 2.02954976668115 2202 52-53 1.9988611367304117 2202 54-55 -0.4712996962990772 2202 56-57 1.3798505538645074 2202 58-59 2.211285301710106 2202 60-61 2.239717503633493 2202 62-63 -1.9700279079154512 2202 64-65 0.26878377028481637 2202 66-67 0.5084418806467959 2202 68-69 1.6866799935129713 2202 70-71 -2.1749951116309134 2202 72-73 -4.551902197367408 2202 74-75 -1.23111091295889 2202 76-77 0.07329973208258522 2202 78-79 1.7352595416404846 2202 80-81 1.879329732296899 2202 82-83 -0.5944604158947371 2202 84-85 -0.08878509877248675 2202 86-87 0.8604180451133701 2202 88-89 1.3134061642298285 2202 90-91 1.6973866790311831 2202 92-93 1.4642412718541102 2202 94-95 1.8513734040303618 2202 96-97 1.2677117327948153 2202 98-99 1.3436148024353614 2202 100-101 1.393280416735216 2305 1 5.774829135877241 2305 2 7.129487885234713 2305 3 6.938799932513259 2305 4 7.915611172640219 2305 5 7.940477841654904 2305 6 6.349308169463626 2305 7 3.0394779591320003 2305 8 6.348051817834644 2305 9 6.51512510160061 2305 10-11 5.074373786862086 2305 12-13 5.171338226848956 2305 14-15 5.388652138934976 2305 16-17 5.285886446048863 2305 18-19 4.480126594811928 2305 20-21 5.425642943339241 2305 22-23 5.166397280088262 2305 24-25 5.464118215999193 2305 26-27 6.208939166981935 2305 28-29 5.45765452643483 2305 30-31 5.633496170734979 2305 32-33 5.835336981165522 2305 34-35 5.658641793090528 2305 36-37 4.962236245680014 2305 38-39 4.784125390357662 2305 40-41 4.29965876303384 2305 42-43 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-23.001984468396582 1209 42-43 -23.779616185533435 1209 44-45 -26.414765123250124 1209 46-47 -20.24699716636752 1209 48-49 -19.731277621873815 1209 50-51 -21.978597142413168 1209 52-53 -24.576616374052087 1209 54-55 -21.640872542902912 1209 56-57 -25.423291775155825 1209 58-59 -25.71108068719932 1209 60-61 -25.463917739742968 1209 62-63 -25.622831358315942 1209 64-65 -25.472744264219187 1209 66-67 -13.36964966929241 1209 68-69 -25.181465416222085 1209 70-71 -25.030201519517544 1209 72-73 -15.383704544333195 1209 74-75 -16.212228357301033 1209 76-77 -13.517450158393906 1209 78-79 -14.341075415217794 1209 80-81 -24.059672116131935 1209 82-83 -16.05774602743418 1209 84-85 -13.404051153044607 1209 86-87 -13.728999992647058 1209 88-89 -11.34984360325235 1209 90-91 -13.29600676923741 1209 92-93 -17.337119396672698 1209 94-95 -22.647702381551895 1209 96-97 -18.4871723128513 1209 98-99 -9.875974560547803 1209 100-101 -5.3779185318391 1212 1 -20.71499052671729 1212 2 -20.526125843677445 1212 3 -21.439326885404135 1212 4 -23.436919368371996 1212 5 -23.628457585919264 1212 6 -24.677451574573602 1212 7 -24.212994408523613 1212 8 -23.87533387151963 1212 9 -24.782722484205088 1212 10-11 -24.509395846645766 1212 12-13 -24.813827567217363 1212 14-15 -25.135780670838148 1212 16-17 -26.16466620083886 1212 18-19 -20.900389485965935 1212 20-21 -26.579301791971986 1212 22-23 -26.753905221366075 1212 24-25 -23.05306370039738 1212 26-27 -25.523172525862748 1212 28-29 -20.338136215690987 1212 30-31 -15.143963038661404 1212 32-33 -25.66582648596653 1212 34-35 -21.83986232059683 1212 36-37 -26.038927221452045 1212 38-39 -26.313896715517846 1212 40-41 -20.949884160592852 1212 42-43 -17.127781433888796 1212 44-45 -13.986193694678693 1212 46-47 -17.332040520422282 1212 48-49 -20.2499269066828 1212 50-51 -12.348786451765974 1212 52-53 -5.087198384634096 1212 54-55 -4.03843727221669 1212 56-57 -4.030434632298679 1212 58-59 -2.7507632268818583 1212 60-61 -14.297251073076302 1212 62-63 -11.699550935035518 1212 64-65 -16.288881830356754 1212 66-67 -12.338703323707282 1212 68-69 -7.767444252200921 1212 70-71 -15.219354958670984 1212 72-73 -11.628604659005171 1212 74-75 -7.669714224482718 1212 76-77 -19.104721568859233 1212 78-79 -9.365176648444503 1212 80-81 -7.829513385973208 1212 82-83 -4.747784653784596 1212 84-85 -4.400651229492592 1212 86-87 -3.393936520701459 1212 88-89 -3.418053312146469 1212 90-91 -3.4641772482443898 1212 92-93 -2.877832978260802 1212 94-95 -2.957226191075705 1212 96-97 -12.723190378717277 1212 98-99 -10.136541482711683 1212 100-101 -6.518105024687191 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 166.0 3 408.0 4 199.0 5 1183.0 6 1449.0 7 1183.0 8 2368.0 9 4702.0 10 6195.0 11 7676.0 12 10513.0 13 13224.0 14 15485.0 15 16349.0 16 17441.0 17 18943.0 18 21545.0 19 23937.0 20 25659.0 21 28783.0 22 32312.0 23 34271.0 24 36367.0 25 37732.0 26 39459.0 27 41201.0 28 41903.0 29 42272.0 30 43740.0 31 45799.0 32 49995.0 33 57972.0 34 66275.0 35 73150.0 36 77792.0 37 72042.0 38 25805.0 39 55.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 30.273168562141862 24.297536069436614 20.574348077082906 24.85494729133862 2 25.03221010361734 30.531472159788276 23.84452384637688 20.591793890217502 3 18.602718814310972 27.230762128975673 29.35956603747253 24.806953019240822 4 21.590613606715475 28.00470021844386 28.080404016909377 22.32428215793129 5 21.35500014392892 28.00408971356013 28.066032455438005 22.574877687072945 6 21.16216042144391 29.326767270418674 27.282216322261878 22.228855985875537 7 22.04898582689329 28.381721283689554 28.347929816732247 21.22136307268491 8 21.59966119192766 28.257713972276793 28.248554084824868 21.89407075097068 9 21.09669450496372 27.140548777055866 29.740142919859064 22.02261379812135 10-11 22.206096466133587 27.424690910087023 28.090395199899937 22.278817423879456 12-13 22.11176619125364 27.83062605874048 27.775880805629306 22.281726944376572 14-15 22.32080488313322 27.889720937692818 27.53507413988486 22.254400039289095 16-17 22.591190708410224 27.80042826807675 27.352345840376778 22.256035183136248 18-19 22.46888181152552 27.916704723715807 27.414950605693534 22.19946285906514 20-21 22.514385236560273 27.946785223193732 27.36369498959355 22.17513455065244 22-23 22.406727605994416 27.790738166771273 27.539590440621076 22.26294378661324 24-25 22.27037701848947 28.008390323374122 27.65750768119376 22.06372497694264 26-27 22.18812049897282 27.9454408383698 27.594232415435172 22.27220624722221 28-29 22.380242649484934 27.98169581237126 27.482195656738455 22.155865881405347 30-31 22.318164915500983 27.995492198873347 27.502383457813785 22.183959427811885 32-33 22.304147327548762 27.990259418048602 27.447475811868927 22.25811744253371 34-35 22.42503452357467 27.82565594791872 27.484415072006314 22.264894456500294 36-37 22.33536498060065 27.964193651222857 27.5169385701507 22.183502798025792 38-39 22.398168766954726 27.90597541178741 27.570579181823256 22.125276639434603 40-41 22.4601680033196 27.871620713469152 27.457208615118734 22.211002668092515 42-43 22.387758144974498 27.990270858861887 27.48482371517007 22.137147280993545 44-45 22.246549513870228 27.89914502051667 27.645397173628822 22.20890829198428 46-47 22.43512637769515 27.849962974136748 27.442727454383746 22.272183193784358 48-49 22.3588396800764 27.88426350839633 27.59742229406435 22.15947451746292 50-51 22.39615026754973 27.935931359655147 27.436557539877278 22.231360832917844 52-53 22.337129689313837 27.944314607209197 27.513812099949515 22.204743603527454 54-55 22.27841310387766 27.9117909797639 27.629868611773507 22.17992730458493 56-57 22.27910845959223 27.888055366864545 27.627907892534726 22.204928281008495 58-59 22.453654609009856 27.911118966262126 27.434654962491656 22.20057146223636 60-61 22.387682988389702 27.887112030307335 27.436863798317024 22.28834118298594 62-63 22.45027486839752 27.93525041405211 27.43586080073402 22.178613916816346 64-65 22.53104127994991 27.840622562003077 27.421733420398102 22.206602737648907 66-67 22.518831666827758 27.905448494559472 27.4305344749791 22.145185363633672 68-69 22.549944873637912 27.891845194266857 27.368096056224996 22.19011387587024 70-71 22.711999566628226 27.80528918537331 27.29344198210975 22.18926926588871 72-73 22.595638304709777 27.940307335745384 27.37394126513229 22.090113094412548 74-75 22.708722936430792 27.906610294753825 27.232622110814603 22.152044658000783 76-77 22.866094759588467 27.863295384086932 27.158405840554945 22.112204015769656 78-79 22.857303001133193 27.834383262864176 27.21787763954721 22.09043609645542 80-81 23.021738151160047 27.824533173942505 27.056802841522536 22.096925833374907 82-83 23.033939051601063 27.821015638420842 27.106547599983305 22.03849770999479 84-85 23.127286536755626 27.87062429579983 26.97673699488434 22.025352172560204 86-87 23.150943414465335 27.88512567098475 26.998647638878015 21.965283275671904 88-89 23.355819504982072 27.76666892172686 26.92976776101516 21.947743812275903 90-91 23.421168999688284 27.80836118219316 26.79057899048185 21.9798908276367 92-93 23.59605618242212 27.7750555424684 26.74948496544105 21.87940330966843 94-95 23.752404074749016 27.74205224047152 26.5893093149042 21.91623436987527 96-97 23.831311133160803 27.836222386921676 26.59779342584186 21.734673054075657 98-99 24.0968133909569 27.77058154225983 26.410082352742233 21.722522714041034 100-101 24.453519317687405 27.6699477850636 26.122471103686017 21.754061793562983 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 69.0 1 76.5 2 90.5 3 93.5 4 102.0 5 132.5 6 166.0 7 221.5 8 294.0 9 393.5 10 522.0 11 666.5 12 864.0 13 1157.5 14 1514.0 15 1874.0 16 2261.0 17 2717.5 18 3355.5 19 4054.0 20 4814.5 21 5781.5 22 6939.5 23 8228.0 24 9726.5 25 11497.0 26 13366.5 27 15616.0 28 17950.0 29 20163.5 30 22488.5 31 24851.5 32 27057.0 33 29150.0 34 31262.0 35 32852.0 36 34011.0 37 34903.5 38 35043.0 39 34714.0 40 34054.5 41 33051.0 42 31723.0 43 30202.0 44 28576.0 45 26976.5 46 25264.5 47 23593.0 48 22056.5 49 20383.5 50 18762.0 51 17401.5 52 16243.0 53 15115.5 54 14342.5 55 13686.5 56 12920.5 57 12503.0 58 12285.5 59 11913.0 60 11528.0 61 11342.5 62 11295.0 63 11203.0 64 11009.0 65 10887.5 66 10835.0 67 10480.5 68 10131.5 69 9749.5 70 9105.0 71 8379.0 72 7622.5 73 6910.0 74 6114.0 75 5120.5 76 4227.0 77 3511.0 78 2853.0 79 2290.5 80 1843.5 81 1454.0 82 1162.0 83 948.5 84 757.0 85 595.0 86 479.0 87 385.5 88 316.5 89 259.5 90 199.5 91 150.5 92 120.5 93 79.0 94 50.0 95 39.0 96 25.0 97 15.5 98 9.5 99 5.0 100 2.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 14.019989377625416 2 11.408044034571 3 10.046931582250977 4 11.984259572208005 5 9.423687895321327 6 5.5418859543237895 7 9.981362560957944 8 10.389744580174787 9 5.635555984742408 10-11 6.581381874366279 12-13 5.541306552073777 14-15 7.584520303220511 16-17 2.7718603640577473 18-19 3.2605378784220944 20-21 3.1912027425039833 22-23 3.220607406692096 24-25 3.620829510887934 26-27 4.507604654531408 28-29 1.8090386751001881 30-31 2.3575877552991167 32-33 3.1809666360871036 34-35 2.1003331562937566 36-37 3.2181449471295447 38-39 1.430833856404809 40-41 1.2104195837960505 42-43 0.5857273912413693 44-45 1.35807059050746 46-47 1.4139346241127904 48-49 1.3090628168606055 50-51 1.6101588527835449 52-53 2.5474868427405726 54-55 1.899232292018734 56-57 1.9100960842064603 58-59 1.6522620829510888 60-61 1.4808555839891844 62-63 0.6464197769301337 64-65 1.286514412630969 66-67 0.589107237699773 68-69 0.8515281734344068 70-71 1.0646999179180145 72-73 0.08531698131427744 74-75 0.10723769977306745 76-77 0.47926222780165134 78-79 0.9354932161653227 80-81 0.31736758244411184 82-83 0.5024383178021341 84-85 0.0664381246680508 86-87 0.10265076529380522 88-89 0.08111631500168992 90-91 0.09149727198107287 92-93 0.18169088889961857 94-95 0.48360774467674184 96-97 0.3902756989039641 98-99 0.06494133552218628 100-101 0.23335425619236153 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1035550.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.3704916443508 #Duplication Level Percentage of deduplicated Percentage of total 1 99.44536085264969 98.81934399677678 2 0.498775823513903 0.9912719760578498 3 0.04225376860349943 0.12596333279869118 4 0.006362575359772134 0.025290089664991564 5 0.005109718579479017 0.025387762370355185 6 0.002137261293648904 0.01274284233133997 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.9313408333735695E-4 0.0 0.0 2 1.9313408333735695E-4 0.0 0.0 3 1.9313408333735695E-4 0.0 0.0 4 1.9313408333735695E-4 0.0 0.0 5 1.9313408333735695E-4 0.0 0.0 6 2.897011250060354E-4 0.0 0.0 7 4.828352083433924E-4 0.0 9.656704166867848E-5 8 6.759692916807493E-4 0.0 1.9313408333735695E-4 9 9.656704166867848E-4 0.0 1.9313408333735695E-4 10-11 0.0011105209791898024 0.0 1.9313408333735695E-4 12-13 0.0013519385833614987 0.0 1.9313408333735695E-4 14-15 0.0013519385833614987 0.0 1.9313408333735695E-4 16-17 0.0013519385833614987 0.0 1.9313408333735695E-4 18-19 0.001400222104195838 0.0 1.9313408333735695E-4 20-21 0.0016899232292018735 9.656704166867848E-5 1.9313408333735695E-4 22-23 0.0018347737917048911 1.448505625030177E-4 1.9313408333735695E-4 24-25 0.002172758437545266 1.9313408333735695E-4 1.9313408333735695E-4 26-27 0.0023658925208826227 1.9313408333735695E-4 1.9313408333735695E-4 28-29 0.0029452947708946938 1.9313408333735695E-4 1.9313408333735695E-4 30-31 0.003862681666747139 1.9313408333735695E-4 1.9313408333735695E-4 32-33 0.005600888416783353 1.9313408333735695E-4 1.9313408333735695E-4 34-35 0.007918497416831635 2.414176041716962E-4 1.9313408333735695E-4 36-37 0.01071894162522331 2.897011250060354E-4 1.9313408333735695E-4 38-39 0.015740427791994593 2.897011250060354E-4 1.9313408333735695E-4 40-41 0.021872434937955675 2.897011250060354E-4 1.9313408333735695E-4 42-43 0.031384288542320504 2.897011250060354E-4 1.9313408333735695E-4 44-45 0.044662256771763796 2.897011250060354E-4 1.9313408333735695E-4 46-47 0.0628651441263097 2.897011250060354E-4 1.9313408333735695E-4 48-49 0.08671720341847328 2.897011250060354E-4 1.9313408333735695E-4 50-51 0.11501134662739607 2.897011250060354E-4 1.9313408333735695E-4 52-53 0.1518516730239969 2.897011250060354E-4 1.9313408333735695E-4 54-55 0.1929409492540196 2.897011250060354E-4 1.9313408333735695E-4 56-57 0.24885326638018446 2.897011250060354E-4 1.9313408333735695E-4 58-59 0.31538795808990394 2.897011250060354E-4 1.9313408333735695E-4 60-61 0.3922070397373376 2.897011250060354E-4 1.9313408333735695E-4 62-63 0.47786200569745546 2.897011250060354E-4 1.9313408333735695E-4 64-65 0.5807059050745981 3.3798464584037466E-4 1.9313408333735695E-4 66-67 0.7019941094104583 3.862681666747139E-4 1.9313408333735695E-4 68-69 0.8338564048090387 3.862681666747139E-4 1.9313408333735695E-4 70-71 0.9815074115204481 3.862681666747139E-4 1.9313408333735695E-4 72-73 1.1428709381488098 3.862681666747139E-4 1.9313408333735695E-4 74-75 1.328376225194341 3.862681666747139E-4 1.9313408333735695E-4 76-77 1.5265800782193037 3.862681666747139E-4 1.9313408333735695E-4 78-79 1.7468495002655593 3.862681666747139E-4 1.9313408333735695E-4 80-81 1.9941094104582104 3.862681666747139E-4 1.9313408333735695E-4 82-83 2.2548404229636425 3.862681666747139E-4 1.9313408333735695E-4 84-85 2.536671334073681 3.862681666747139E-4 2.414176041716962E-4 86-87 2.8474723576843224 3.862681666747139E-4 2.897011250060354E-4 88-89 3.183332528607986 3.862681666747139E-4 2.897011250060354E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGTGT 1870 0.0 6.178403 94-95 GTGTAGG 1525 0.0 6.0906224 94-95 TGTAGGG 1455 0.0 6.0722446 94-95 CGTGTAG 1495 0.0 5.9097767 92-93 TCGTGTA 1595 0.0 5.6812897 92-93 GTAGGGA 985 1.4769103E-7 5.519791 94-95 GCGTCGT 2980 0.0 5.2454224 92-93 TAGGGGA 610 0.0028808895 5.1993117 92-93 AGCGTCG 3190 0.0 5.184177 94-95 GTAGGGG 700 5.375906E-4 5.17809 94-95 TCGGAAG 4495 0.0 5.039854 94-95 >>END_MODULE