FastQCFastQC Report
Thu 11 Sep 2014
NYCLib9_ACTTGA_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNYCLib9_ACTTGA_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1035550
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTGT18700.06.17840394-95
GTGTAGG15250.06.090622494-95
TGTAGGG14550.06.072244694-95
CGTGTAG14950.05.909776792-93
TCGTGTA15950.05.681289792-93
GTAGGGA9851.4769103E-75.51979194-95
GCGTCGT29800.05.245422492-93
TAGGGGA6100.00288088955.199311792-93
AGCGTCG31900.05.18417794-95
GTAGGGG7005.375906E-45.1780994-95
TCGGAAG44950.05.03985494-95
Produced by FastQC (version 0.11.2)