##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename NYCLib9_ACTTGA_L007_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1035550 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.266164839940128 33.0 33.0 33.0 27.0 33.0 2 31.665017623485106 33.0 33.0 33.0 27.0 33.0 3 31.826055719183042 33.0 33.0 33.0 27.0 33.0 4 35.65909516681956 37.0 37.0 37.0 33.0 37.0 5 35.17079716092898 37.0 37.0 37.0 33.0 37.0 6 34.957250736323694 37.0 33.0 37.0 33.0 37.0 7 34.756511998454926 37.0 33.0 37.0 27.0 37.0 8 34.79164984790691 37.0 33.0 37.0 27.0 37.0 9 34.69950750808749 37.0 33.0 37.0 27.0 37.0 10-11 34.54964753029791 37.0 33.0 37.0 27.0 37.0 12-13 34.5475230553812 37.0 33.0 37.0 27.0 37.0 14-15 35.485352711119695 37.0 33.0 40.0 27.0 40.0 16-17 35.386244990584714 37.0 33.0 40.0 27.0 40.0 18-19 35.593906619670705 37.0 33.0 40.0 27.0 40.0 20-21 35.28371927960987 37.0 33.0 40.0 27.0 40.0 22-23 35.311301240886486 37.0 33.0 40.0 27.0 40.0 24-25 35.15063492829897 37.0 33.0 40.0 27.0 40.0 26-27 34.781586113659415 37.0 33.0 40.0 27.0 40.0 28-29 34.41479069093718 37.0 33.0 40.0 27.0 40.0 30-31 34.299200424894984 37.0 33.0 40.0 24.5 40.0 32-33 34.305231519482405 37.0 33.0 40.0 24.5 40.0 34-35 34.14945198203853 37.0 33.0 38.5 24.5 40.0 36-37 33.842939983583605 37.0 33.0 37.0 22.0 40.0 38-39 33.762496740862346 37.0 33.0 37.0 22.0 40.0 40-41 33.47218531215296 37.0 33.0 37.0 22.0 40.0 42-43 33.099451499203326 37.0 33.0 37.0 22.0 40.0 44-45 32.99201583699484 37.0 33.0 37.0 22.0 40.0 46-47 32.84790884071266 37.0 33.0 37.0 22.0 40.0 48-49 32.877635073149534 37.0 33.0 37.0 22.0 40.0 50-51 33.18810294046642 37.0 33.0 37.0 22.0 40.0 52-53 33.05129689536961 37.0 33.0 37.0 22.0 40.0 54-55 32.700519530684176 37.0 33.0 37.0 22.0 40.0 56-57 32.75151899956545 37.0 33.0 37.0 22.0 40.0 58-59 32.46253102216214 37.0 33.0 37.0 22.0 40.0 60-61 32.36977210178166 37.0 30.0 37.0 22.0 40.0 62-63 32.372362029839216 37.0 30.0 37.0 22.0 40.0 64-65 32.04070831925064 33.0 27.0 37.0 22.0 40.0 66-67 31.689971029887502 33.0 27.0 37.0 18.5 40.0 68-69 31.529764859253536 33.0 27.0 37.0 22.0 40.0 70-71 31.374671430640724 33.0 27.0 37.0 18.5 40.0 72-73 31.148321664815796 33.0 27.0 37.0 15.0 37.0 74-75 30.79765680073391 33.0 27.0 37.0 15.0 37.0 76-77 29.710425377818552 33.0 27.0 37.0 15.0 37.0 78-79 30.62355559847424 33.0 27.0 37.0 15.0 37.0 80-81 30.333898411472163 33.0 27.0 37.0 15.0 37.0 82-83 30.313269760030902 33.0 27.0 37.0 15.0 37.0 84-85 30.17468350152093 33.0 27.0 37.0 15.0 37.0 86-87 29.983856887644247 33.0 27.0 37.0 15.0 37.0 88-89 29.845688281589496 33.0 27.0 37.0 15.0 37.0 90-91 29.757737434213702 33.0 27.0 37.0 15.0 37.0 92-93 29.546395635169716 33.0 27.0 35.0 15.0 37.0 94-95 29.459042054946647 33.0 27.0 33.0 15.0 37.0 96-97 29.32181256337212 33.0 27.0 33.0 15.0 37.0 98-99 29.055501424363865 33.0 27.0 33.0 15.0 37.0 100-101 28.2395195789677 33.0 24.5 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 1.027775489208711 2306 2 0.566766612921441 2306 3 0.5034303215681284 2306 4 0.3089316909674906 2306 5 0.6823176386806011 2306 6 0.7451314367855773 2306 7 0.8835622449572327 2306 8 0.9535716164221881 2306 9 0.9029027659189452 2306 10-11 1.141892391257592 2306 12-13 1.3853396345211664 2306 14-15 1.3768506368637148 2306 16-17 1.4392679386209508 2306 18-19 1.410159838805832 2306 20-21 1.5769918067409492 2306 22-23 1.245737932328339 2306 24-25 1.2015276991005592 2306 26-27 1.2186942262953622 2306 28-29 1.492265053477709 2306 30-31 1.5168720191455805 2306 32-33 0.9594169925499898 2306 34-35 0.928511839508964 2306 36-37 1.4051402668887008 2306 38-39 0.9933304475632738 2306 40-41 1.2421442248739183 2306 42-43 1.1162598515114652 2306 44-45 1.0575617750748805 2306 46-47 1.0038576397167347 2306 48-49 1.0244682729666366 2306 50-51 0.8257272366985688 2306 52-53 0.7242739600810637 2306 54-55 1.381395575037132 2306 56-57 1.0504226082882937 2306 58-59 0.8500162953551609 2306 60-61 0.7892292569328561 2306 62-63 0.5141015003992209 2306 64-65 0.6918768084220375 2306 66-67 0.8306160408321794 2306 68-69 0.6327967394151024 2306 70-71 0.5600110328797925 2306 72-73 0.23308564586025682 2306 74-75 0.5666482863609872 2306 76-77 0.700327411174289 2306 78-79 0.5702326473512649 2306 80-81 1.0531329722934828 2306 82-83 0.9011948571966997 2306 84-85 0.6507100426668408 2306 86-87 0.5263141798098339 2306 88-89 0.43345445971937124 2306 90-91 0.04892572444989085 2306 92-93 -0.08182322474604575 2306 94-95 -0.19515748943384637 2306 96-97 -0.23324087389489634 2306 98-99 -0.0865392428138847 2306 100-101 0.05966201345427535 2307 1 0.5218997422566076 2307 2 0.33028427041333686 2307 3 0.4012157795191804 2307 4 0.3841212779786076 2307 5 0.5957590469702154 2307 6 0.6767512350567131 2307 7 0.8607372120126087 2307 8 0.854482048473372 2307 9 0.9447735235210715 2307 10-11 1.2520117792748664 2307 12-13 1.4051548959647633 2307 14-15 1.2245563232308498 2307 16-17 1.324845650647596 2307 18-19 1.2132508322832152 2307 20-21 1.4954957062246095 2307 22-23 1.5295345624451073 2307 24-25 1.4937649915908437 2307 26-27 1.2717269968764313 2307 28-29 1.6616118381648874 2307 30-31 1.774567179807626 2307 32-33 1.5173058842015337 2307 34-35 1.3906839472192516 2307 36-37 1.64414090506137 2307 38-39 1.255796570539971 2307 40-41 1.4431190957889939 2307 42-43 1.4124241851182973 2307 44-45 1.147565463215642 2307 46-47 1.0117351354277204 2307 48-49 1.0192315274815016 2307 50-51 1.183853785510486 2307 52-53 1.3745569938047524 2307 54-55 1.6146474791951988 2307 56-57 1.5390403428581934 2307 58-59 1.4791066092200893 2307 60-61 1.1980770651826802 2307 62-63 0.8249400758652143 2307 64-65 1.0719560827285406 2307 66-67 1.2707276796098732 2307 68-69 0.9908033200527804 2307 70-71 1.058802027185969 2307 72-73 0.9840930470056506 2307 74-75 1.1347856509564949 2307 76-77 1.3238086430961822 2307 78-79 1.2383627599846 2307 80-81 1.7686625312913016 2307 82-83 1.433720943540635 2307 84-85 1.1788607094329606 2307 86-87 1.2535475769604325 2307 88-89 1.1750198061145234 2307 90-91 0.9340430404779703 2307 92-93 0.9191802396198838 2307 94-95 0.9113672045333487 2307 96-97 0.909583620332544 2307 98-99 1.0374804990991393 2307 100-101 1.0241464611033955 1101 1 0.989406430494828 1101 2 0.5607989210916777 1101 3 0.29450829609438145 1101 4 0.2558562016851056 1101 5 0.678689182638351 1101 6 0.508253524390426 1101 7 0.6959263331833938 1101 8 0.8096542507806817 1101 9 0.9496315636822246 1101 10-11 0.8710676009500418 1101 12-13 1.1851749871151398 1101 14-15 1.1565912395353521 1101 16-17 1.2446609178070318 1101 18-19 0.891967853717297 1101 20-21 1.2590281534324674 1101 22-23 1.0016931979387707 1101 24-25 1.2513335394236478 1101 26-27 1.1272419119120158 1101 28-29 1.2587437735771232 1101 30-31 1.3018626994810845 1101 32-33 0.8007590242368465 1101 34-35 1.0301489939047386 1101 36-37 1.1337702762083595 1101 38-39 0.9193680368767261 1101 40-41 0.8286446908571463 1101 42-43 1.0727991494300753 1101 44-45 0.8342890195607495 1101 46-47 0.6801810320746142 1101 48-49 0.6532007985957122 1101 50-51 0.8012646706842759 1101 52-53 1.1016271753653157 1101 54-55 1.341620800262362 1101 56-57 0.9471389798322534 1101 58-59 1.0955925612762627 1101 60-61 0.7147123262090318 1101 62-63 0.4029054767894067 1101 64-65 0.5310737639983074 1101 66-67 0.5517716792292013 1101 68-69 0.45986104510009795 1101 70-71 0.6065549906306451 1101 72-73 0.628963247599927 1101 74-75 0.7988259812973055 1101 76-77 0.6297293993693778 1101 78-79 0.7818185435923333 1101 80-81 1.3982929598672627 1101 82-83 1.0137888304803298 1101 84-85 0.8245978960374529 1101 86-87 0.7078208589027177 1101 88-89 0.6726032636957591 1101 90-91 0.5602553299174282 1101 92-93 0.7151649703456009 1101 94-95 0.9271444676956939 1101 96-97 0.8316069049183987 1101 98-99 0.7763778121721359 1101 100-101 0.692395781705283 2201 1 1.5144607221595088 2201 2 0.8596310541261332 2201 3 0.29860807193260186 2201 4 0.2927808928664817 2201 5 0.4699567300541361 2201 6 0.4773102961428606 2201 7 0.594401715132804 2201 8 0.5624130304503225 2201 9 0.40036080487075765 2201 10-11 0.6437904511432038 2201 12-13 0.9177328160844738 2201 14-15 0.8530119641661216 2201 16-17 0.891470348499702 2201 18-19 0.8137360291040139 2201 20-21 1.1345388766324191 2201 22-23 0.7996013393037771 2201 24-25 0.706278611839025 2201 26-27 0.777847466672668 2201 28-29 1.2273980080090112 2201 30-31 1.2543556646167744 2201 32-33 0.8022054241350958 2201 34-35 0.931863116096757 2201 36-37 0.9974284056498632 2201 38-39 0.42253714388615293 2201 40-41 0.6729066262794703 2201 42-43 0.8547581007980938 2201 44-45 0.7332645525411507 2201 46-47 0.6185359071958416 2201 48-49 0.6492841676995411 2201 50-51 0.5002007766285388 2201 52-53 0.6258763923888893 2201 54-55 1.0471809662374625 2201 56-57 0.8864019486244885 2201 58-59 1.0672648717323767 2201 60-61 0.6245830754816879 2201 62-63 0.5804155322734204 2201 64-65 0.46596033177942076 2201 66-67 0.4365398069219246 2201 68-69 0.5730980365628717 2201 70-71 0.7981687964524937 2201 72-73 0.5276783713658446 2201 74-75 0.40314038011449327 2201 76-77 0.4748514165074198 2201 78-79 0.5875643981892402 2201 80-81 1.04356759779035 2201 82-83 0.6634405053344636 2201 84-85 0.7114140038894767 2201 86-87 0.744915976627393 2201 88-89 0.6935905293990068 2201 90-91 0.6571603075807424 2201 92-93 0.5891507575349912 2201 94-95 0.37721425990885393 2201 96-97 0.4363225692162658 2201 98-99 0.5955679681314976 2201 100-101 0.576838318389914 2304 1 -1.7230945060009049 2304 2 -0.3809679999075115 2304 3 -0.25302664998318036 2304 4 -0.01723383991502203 2304 5 0.32958171235976863 2304 6 0.5750195581831363 2304 7 0.6572244148458211 2304 8 0.5564234390853287 2304 9 0.9032350021002387 2304 10-11 0.8527978507479546 2304 12-13 1.0437645259649244 2304 14-15 1.245553602264792 2304 16-17 1.094578309025657 2304 18-19 0.7921263679421742 2304 20-21 1.1153894239121485 2304 22-23 0.957414102495072 2304 24-25 0.9237670482267006 2304 26-27 1.1690399837165941 2304 28-29 1.544783480996145 2304 30-31 1.7701097766286225 2304 32-33 1.0910644204238338 2304 34-35 0.984164490444634 2304 36-37 0.9339350994571092 2304 38-39 1.1458056071139069 2304 40-41 1.13996189674819 2304 42-43 0.79715395316029 2304 44-45 0.9834217259348392 2304 46-47 1.0750995076567236 2304 48-49 1.0296117526384236 2304 50-51 1.0828320688033983 2304 52-53 1.1547176112794304 2304 54-55 1.6135247453738515 2304 56-57 1.1850223023219542 2304 58-59 1.0816212279034971 2304 60-61 0.7651328620817353 2304 62-63 0.34422682297737595 2304 64-65 0.1989391452013507 2304 66-67 0.6046058110483692 2304 68-69 0.7085079566357528 2304 70-71 0.47352725064128975 2304 72-73 0.35179363343617 2304 74-75 0.5414292422740203 2304 76-77 0.6782974481065871 2304 78-79 0.7509756974339403 2304 80-81 1.2208481145369277 2304 82-83 0.9573041397210744 2304 84-85 0.7610151591040335 2304 86-87 0.6767236802193324 2304 88-89 0.5594059686897488 2304 90-91 0.26395438947855965 2304 92-93 0.3952320037242778 2304 94-95 0.34927021442781836 2304 96-97 0.22131964717506136 2304 98-99 0.5410804008434944 2304 100-101 0.5247378096621453 1102 1 1.5461368259594117 1102 2 0.036829626260921344 1102 3 0.3907132894331333 1102 4 0.19031992817610188 1102 5 0.271363081514302 1102 6 -0.012492996478592033 1102 7 -4.964690423164978 1102 8 -1.182857009896054 1102 9 0.24708714096131956 1102 10-11 0.7541188550520488 1102 12-13 0.9649093447230896 1102 14-15 0.8861992353367469 1102 16-17 0.4940502171362908 1102 18-19 0.8670775191555222 1102 20-21 1.1597525278233007 1102 22-23 0.8579084570929325 1102 24-25 1.0588437331476541 1102 26-27 0.9165668480390323 1102 28-29 1.0387243954087069 1102 30-31 1.2421719427520728 1102 32-33 0.9621554058252855 1102 34-35 1.2040797054180317 1102 36-37 1.3993628386827694 1102 38-39 0.9999024360093216 1102 40-41 1.1865768788023061 1102 42-43 1.0977658432031063 1102 44-45 1.0796296658457365 1102 46-47 1.0318363083000222 1102 48-49 0.9621051823295872 1102 50-51 0.8949776084953527 1102 52-53 0.9802500565990542 1102 54-55 1.3395736351845926 1102 56-57 1.2617042110149654 1102 58-59 1.0620934753952156 1102 60-61 0.9434928280805153 1102 62-63 0.7095215064564542 1102 64-65 0.49059473106117935 1102 66-67 0.7198011617282951 1102 68-69 0.8275820917518821 1102 70-71 0.7810057983426901 1102 72-73 0.7318219888264039 1102 74-75 0.7722281763362631 1102 76-77 0.8411926241042273 1102 78-79 0.8048677214620064 1102 80-81 1.4215682163684633 1102 82-83 1.2988863268901767 1102 84-85 0.8220684683278279 1102 86-87 0.724679404040586 1102 88-89 0.660886507924122 1102 90-91 0.4397514975385839 1102 92-93 0.5911376563558299 1102 94-95 0.5799000317099754 1102 96-97 0.21865709783498843 1102 98-99 0.23798239372480978 1102 100-101 0.45036257352262155 2202 1 1.5485769746127893 2202 2 1.0526994033828814 2202 3 0.47202035337442894 2202 4 -0.19808817547936286 2202 5 0.1980093007678292 2202 6 0.5713100183246027 2202 7 1.0575809956857825 2202 8 -4.119175715012759 2202 9 -0.43353626090825514 2202 10-11 0.5708823331403252 2202 12-13 0.25230354852759973 2202 14-15 0.29144198421845857 2202 16-17 0.2702482720042312 2202 18-19 0.8035487915293515 2202 20-21 1.400071772103722 2202 22-23 -2.394194791523063 2202 24-25 -2.3855245564821956 2202 26-27 -1.024306306585025 2202 28-29 0.735031618564058 2202 30-31 1.4870544474148844 2202 32-33 1.14341226323657 2202 34-35 -1.6918167145534184 2202 36-37 0.22881181274139095 2202 38-39 0.5570075720074925 2202 40-41 1.3509987818338374 2202 42-43 1.508316603272469 2202 44-45 1.680111357929455 2202 46-47 1.0158795044650404 2202 48-49 1.3746697683622173 2202 50-51 -3.3079145844201534 2202 52-53 0.07968186336326966 2202 54-55 1.752970153312269 2202 56-57 1.5087821693280787 2202 58-59 1.8077097559136917 2202 60-61 1.6333126194080805 2202 62-63 1.3297548617087784 2202 64-65 1.2598136454438986 2202 66-67 1.442843980416864 2202 68-69 1.0804495678442727 2202 70-71 1.610037260586239 2202 72-73 1.443076320453514 2202 74-75 0.9462256456056295 2202 76-77 1.7319526130859941 2202 78-79 1.7525356065026543 2202 80-81 2.2521213321875706 2202 82-83 2.0092863127927743 2202 84-85 1.6473832870757619 2202 86-87 1.5822857706243063 2202 88-89 1.6174380736475307 2202 90-91 1.3930923494852792 2202 92-93 1.3051932279358418 2202 94-95 1.4012830361194304 2202 96-97 1.410658894635187 2202 98-99 1.4992719843097255 2202 100-101 1.5027712039756658 2305 1 1.3739612037190376 2305 2 0.7205919041812834 2305 3 0.21470809148464198 2305 4 -0.06944118452580739 2305 5 0.23989196693555925 2305 6 0.4385586336159193 2305 7 0.4668837863939572 2305 8 -0.2416731047387657 2305 9 0.5366071509322268 2305 10-11 0.3057537657210574 2305 12-13 0.7499002684535228 2305 14-15 0.5235359347270148 2305 16-17 0.6243315362499899 2305 18-19 0.7302186410635869 2305 20-21 0.8411629150220108 2305 22-23 0.6170914848337219 2305 24-25 0.765565952013155 2305 26-27 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-5.6353395118423855 1208 36-37 -3.0994768502284202 1208 38-39 -0.4476183136979941 1208 40-41 -0.6970449643152676 1208 42-43 0.3305757298898442 1208 44-45 0.2228630138136225 1208 46-47 -0.00698132424722786 1208 48-49 -1.2257287991054397 1208 50-51 0.0988832051670343 1208 52-53 -1.2655064453243448 1208 54-55 -0.6360875330709739 1208 56-57 -6.144340905225214 1208 58-59 -1.9196768352754674 1208 60-61 -1.0259593691932736 1208 62-63 0.5283329193181387 1208 64-65 0.20760034445807207 1208 66-67 0.8726768516429892 1208 68-69 0.7173969646403329 1208 70-71 -3.9732941473003933 1208 72-73 -0.2924914937793801 1208 74-75 -5.207183357783158 1208 76-77 -0.9079214626995871 1208 78-79 -3.549474559855941 1208 80-81 1.0450530173385246 1208 82-83 0.5725208707102141 1208 84-85 -2.4651542591349624 1208 86-87 -0.549980865235213 1208 88-89 -4.702834954695046 1208 90-91 -0.07506076203659262 1208 92-93 -2.054134598619921 1208 94-95 -4.610585417361776 1208 96-97 -2.9486265720930938 1208 98-99 -5.961384400471545 1208 100-101 -3.7122270631223024 1209 1 1.8014181432696184 1209 2 1.3384327657662993 1209 3 0.9246066309573671 1209 4 0.3543484004526434 1209 5 0.5087280795397149 1209 6 1.285485288736389 1209 7 0.8457449599916629 1209 8 0.6810182645912661 1209 9 -0.2494453997372048 1209 10-11 -3.273058966944717 1209 12-13 -0.41996814474098443 1209 14-15 -0.829538737301327 1209 16-17 -0.23474295440134796 1209 18-19 -16.759737054284976 1209 20-21 -13.87086127447715 1209 22-23 -3.409463657093788 1209 24-25 -2.026582635568708 1209 26-27 -1.120214930009233 1209 28-29 -3.2019039253870787 1209 30-31 -0.4793505342062474 1209 32-33 0.44043298953556587 1209 34-35 -3.2062995878880116 1209 36-37 -4.392965443384313 1209 38-39 -1.4914732725066067 1209 40-41 -10.185099463681556 1209 42-43 -4.144652010921305 1209 44-45 -2.034783136376493 1209 46-47 -2.0671088591014737 1209 48-49 -2.793421289599735 1209 50-51 -2.331501379750584 1209 52-53 -0.25160039335982987 1209 54-55 -1.8400522035399192 1209 56-57 -0.1372987632733782 1209 58-59 -1.669651090662029 1209 60-61 -1.5574925599410534 1209 62-63 -0.9596285033688048 1209 64-65 0.21374934635921505 1209 66-67 0.381731034152498 1209 68-69 -1.0063633332050443 1209 70-71 -1.2648142677059653 1209 72-73 -0.18747921557913116 1209 74-75 0.8578653589468814 1209 76-77 -16.049497033041792 1209 78-79 -16.68066910991931 1209 80-81 -21.986676228542336 1209 82-83 -9.988967167946313 1209 84-85 -2.6260283877788133 1209 86-87 -1.6184140084544758 1209 88-89 -1.5980702208547406 1209 90-91 -6.46867609790478 1209 92-93 -3.9319011543867113 1209 94-95 -0.22555690025151165 1209 96-97 0.4414063017979082 1209 98-99 1.6524444176526636 1209 100-101 1.2820081238232 1212 1 -28.651053339620116 1212 2 -16.300350504195677 1212 3 -5.748407230269422 1212 4 -1.290832358796557 1212 5 -0.3671747108117458 1212 6 -2.633811792157651 1212 7 -0.4574215960266059 1212 8 -1.553385340534767 1212 9 -0.0154441524279747 1212 10-11 0.5172069915721949 1212 12-13 0.02954097872250827 1212 14-15 0.030128915141993673 1212 16-17 0.10691415626716605 1212 18-19 -1.1433651147191242 1212 20-21 -0.6746062198570826 1212 22-23 -0.8376025510121536 1212 24-25 -0.027447704874838053 1212 26-27 -0.786242853641717 1212 28-29 0.2912962275088873 1212 30-31 -0.6989473716738104 1212 32-33 -0.06206162892863176 1212 34-35 -0.553262792265663 1212 36-37 -1.4238816121331794 1212 38-39 -0.3095974803646584 1212 40-41 -0.31781216008246815 1212 42-43 0.21871230396391894 1212 44-45 0.7289724714855836 1212 46-47 -2.680608969749869 1212 48-49 -1.2682387197403564 1212 50-51 -0.15778842181709507 1212 52-53 -0.7358933500862506 1212 54-55 -0.5556807870392255 1212 56-57 -1.2009215930559023 1212 58-59 -0.18378893845006772 1212 60-61 0.38241530011889324 1212 62-63 -5.138672702374983 1212 64-65 -4.18835699681177 1212 66-67 -1.442700132483992 1212 68-69 -0.2411843845660222 1212 70-71 -1.189613591744834 1212 72-73 -1.3332735704989602 1212 74-75 -0.7560672863545861 1212 76-77 0.7847868706337451 1212 78-79 -1.4423978402792237 1212 80-81 -0.27986431070380036 1212 82-83 0.24407345007529102 1212 84-85 -3.4088959919391932 1212 86-87 -2.192804939309287 1212 88-89 -1.1219652457003342 1212 90-91 0.2235503490745181 1212 92-93 -1.8722314901543093 1212 94-95 -1.8193817136580748 1212 96-97 0.027158811504790492 1212 98-99 -0.585731292005935 1212 100-101 0.033879087671348174 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 1.0 11 10.0 12 34.0 13 116.0 14 315.0 15 736.0 16 1411.0 17 2344.0 18 3362.0 19 5182.0 20 7138.0 21 9391.0 22 12474.0 23 15625.0 24 19711.0 25 24358.0 26 29645.0 27 35785.0 28 42706.0 29 50455.0 30 59155.0 31 68776.0 32 79516.0 33 90521.0 34 101215.0 35 110963.0 36 117243.0 37 107865.0 38 39410.0 39 85.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 28.82329880239346 24.018961698186807 22.651116232330416 24.506623267089314 2 24.446511035014222 29.862426872630422 25.211825533402475 20.479236558952877 3 18.602764727752053 26.252553003490913 30.974704141297575 24.169978127459455 4 21.645137023936094 27.497588235805352 29.24564651213994 21.61162822811861 5 20.900777364685432 27.941576939790448 28.8096180773502 22.34802761817392 6 20.736130558640337 29.62802375549225 27.74940852686978 21.886437158997634 7 21.43295833132152 28.620153541596256 28.874221428226548 21.07266669885568 8 21.22408382019217 28.387813239341416 28.630872483221477 21.75723045724494 9 20.77253633334943 27.035584954854908 30.064893052001352 22.12698565979431 10-11 21.699097098160397 27.443387571821738 28.5873207474289 22.27019458258896 12-13 21.89423030867193 27.8111478760087 28.047590410119177 22.247031405200186 14-15 21.953889237603207 27.994254261020714 27.746656366182222 22.305200135193857 16-17 22.099951957780785 27.751793133583924 27.73933595513484 22.408918953500443 18-19 22.212612281600805 27.91864478887675 27.63563230616014 22.23311062336231 20-21 22.056829704022018 27.802230698662544 27.84032639660084 22.300613200714597 22-23 22.06098208681377 27.758244411182464 27.85471488580947 22.326058616194295 24-25 21.99642701945826 27.78581430157887 27.9486746173531 22.269084061609774 26-27 22.13026893921105 27.87224180387234 27.821157838829606 22.176331418087006 28-29 22.232002317609002 27.81628120322534 27.729660566848537 22.222055912317128 30-31 22.17314470571194 27.819564482642072 27.776881850224516 22.230408961421467 32-33 22.24991550383854 27.718893341702476 27.70942977161895 22.321761382840037 34-35 22.299116411568733 27.686157114576794 27.70947805513978 22.305248418714694 36-37 22.234706194775722 27.817971126454545 27.796291825599923 22.15103085316981 38-39 22.267249287818068 27.71614118101492 27.808797257496014 22.207812273670996 40-41 22.253472978342984 27.726706263118277 27.67740786002923 22.342412898509508 42-43 22.044469122688426 27.812080536912752 27.912896528414855 22.23055381198397 44-45 22.17681425329535 27.615470040075323 27.902853556081308 22.30486215054802 46-47 22.212691890492625 27.73833637112657 27.80155531150179 22.247416426879013 48-49 22.241514171213364 27.73526145526532 27.84829317753851 22.17493119598281 50-51 22.260199893776253 27.704070300806332 27.779006325141232 22.25672348027618 52-53 22.175811006620158 27.73107417849171 27.81388045670439 22.279234358183746 54-55 22.066380789270763 27.71027850110886 28.000807319568665 22.22253339005172 56-57 22.240350194439802 27.743764358464528 27.76626471818058 22.249620728915087 58-59 22.18450867186118 27.685628479159597 27.855266960706704 22.27459588827252 60-61 22.26655239428799 27.73385802059317 27.744094250570356 22.255495334548485 62-63 22.329568307148108 27.68527902605357 27.75500113708845 22.230151529709875 64-65 22.337959945187542 27.673048899550086 27.754939253387878 22.234051901874498 66-67 22.28615216581998 27.79624696465553 27.783209891565352 22.134390977959136 68-69 22.364718930875522 27.717149613013596 27.659544674730068 22.25858678138081 70-71 22.44670428707523 27.66852772931847 27.567129349085167 22.317638634521128 72-73 22.292106023295602 27.82343490823867 27.67707662121992 22.20738244724581 74-75 22.275133808668095 27.732112148088035 27.67085966030955 22.321894382934325 76-77 22.222931612224208 27.840381445453065 27.651554724924353 22.28513221739837 78-79 22.310929908159782 27.896037703690364 27.537773460151794 22.25525892799806 80-81 22.30661754752374 27.78185492448415 27.593983871903465 22.317543656088645 82-83 22.46583808944385 27.78234879431391 27.394810286718624 22.357002829523616 84-85 22.29014415706384 27.797325453101273 27.55184077295806 22.36068961687683 86-87 22.392969081593282 27.843852654339734 27.397056544116833 22.36612171995015 88-89 22.405062374303768 27.833304763721262 27.37709232169544 22.38454054027953 90-91 22.44965473809066 27.85484607858005 27.24782454571183 22.447674637617464 92-93 22.33749326369426 27.79979216444505 27.3138123723965 22.548902199464184 94-95 22.40583850523758 27.80919874599431 27.232417940085107 22.552544808683002 96-97 22.369748345522105 27.94661245500134 27.159126850950916 22.52451234852564 98-99 22.430402069865693 27.889054735257208 27.08972460220614 22.590818592670963 100-101 22.45016614103227 27.751226440013692 27.004666242682283 22.793941176271748 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 2.0 3 10.0 4 18.5 5 30.5 6 56.0 7 92.0 8 134.0 9 160.5 10 216.0 11 307.5 12 382.0 13 499.5 14 684.0 15 864.0 16 1128.5 17 1522.0 18 1952.5 19 2469.0 20 3172.5 21 4071.5 22 5100.5 23 6346.5 24 7858.5 25 9538.0 26 11485.5 27 13695.5 28 16047.0 29 18670.0 30 21327.5 31 23867.5 32 26422.5 33 28811.0 34 31170.5 35 33162.5 36 34530.0 37 35555.5 38 35787.5 39 35648.0 40 35389.5 41 34523.5 42 33344.5 43 31903.0 44 30057.0 45 28128.5 46 26275.5 47 24613.0 48 23133.0 49 21498.0 50 19828.5 51 18312.0 52 16981.0 53 15870.0 54 14869.0 55 14016.0 56 13421.0 57 12931.5 58 12588.0 59 12336.5 60 12088.5 61 12061.5 62 12077.0 63 12053.5 64 12124.0 65 12202.5 66 12150.0 67 11975.0 68 11601.5 69 11261.5 70 10874.5 71 10047.5 72 9013.0 73 7784.0 74 6544.5 75 5396.0 76 4141.0 77 2971.0 78 2119.5 79 1499.5 80 973.0 81 577.0 82 356.5 83 253.5 84 178.5 85 122.5 86 88.0 87 55.0 88 42.0 89 37.5 90 25.5 91 14.0 92 10.5 93 9.0 94 4.0 95 1.5 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7322678769735889 2 0.0027038771667229973 3 4.8283520834339243E-4 4 9.656704166867849E-5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.20341847327507123 14-15 0.0 16-17 2.4141760417169622E-4 18-19 0.1274684950026556 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0018830573125392302 42-43 0.0 44-45 0.0 46-47 0.025010863792187723 48-49 0.0 50-51 0.0 52-53 4.8283520834339245E-5 54-55 0.0023658925208826227 56-57 0.0010622374583554631 58-59 0.09627734054367244 60-61 0.001207088020858481 62-63 0.001013953937521124 64-65 0.0018347737917048914 66-67 0.004007532229250157 68-69 0.004683501520930906 70-71 0.0029452947708946933 72-73 0.04046159045917628 74-75 0.047317850417652456 76-77 0.17362754092028393 78-79 1.448505625030177E-4 80-81 0.12843416541934238 82-83 0.0038626816667471395 84-85 0.004586934479262228 86-87 0.0064217082709671185 88-89 0.006518275312635798 90-91 0.024045193375500942 92-93 0.011201776833566704 94-95 0.014291922166964415 96-97 0.009898121771039544 98-99 0.011636328521075757 100-101 0.012988267104437255 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1035550.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.78356759114128 #Duplication Level Percentage of deduplicated Percentage of total 1 99.02647076363863 97.82188067992071 2 0.8192123170701202 1.6184943058958334 3 0.089251066498541 0.2644961628012021 4 0.047997993444178315 0.18965652118528578 5 0.012848903835520247 0.06346302802540943 6 0.002149744850249059 0.012741567943097148 7 0.0018497372440278907 0.012790655084989635 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 2.1947341873486303E-4 0.016477079143481184 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 9.656704166867848E-5 0.0 0.0 2 9.656704166867848E-5 0.0 0.0 3 1.9313408333735695E-4 0.0 0.0 4 1.9313408333735695E-4 0.0 0.0 5 2.897011250060354E-4 0.0 0.0 6 4.828352083433924E-4 9.656704166867848E-5 0.0 7 6.759692916807493E-4 9.656704166867848E-5 0.0 8 8.691033750181063E-4 9.656704166867848E-5 0.0 9 0.0011588045000241417 9.656704166867848E-5 0.0 10-11 0.0014967891458645163 9.656704166867848E-5 0.0 12-13 0.0018830573125392302 9.656704166867848E-5 0.0 14-15 0.0019313408333735695 9.656704166867848E-5 0.0 16-17 0.001979624354207909 9.656704166867848E-5 4.828352083433924E-5 18-19 0.0020761913958765874 9.656704166867848E-5 9.656704166867848E-5 20-21 0.0023658925208826227 9.656704166867848E-5 9.656704166867848E-5 22-23 0.002607310125054319 9.656704166867848E-5 9.656704166867848E-5 24-25 0.0029935782917290326 9.656704166867848E-5 9.656704166867848E-5 26-27 0.0032832794167350684 9.656704166867848E-5 9.656704166867848E-5 28-29 0.004152382791753175 9.656704166867848E-5 9.656704166867848E-5 30-31 0.005118053208439959 2.414176041716962E-4 9.656704166867848E-5 32-33 0.007242528125150886 2.897011250060354E-4 9.656704166867848E-5 34-35 0.009753271208536526 2.897011250060354E-4 1.448505625030177E-4 36-37 0.01274684950026556 2.897011250060354E-4 1.9313408333735695E-4 38-39 0.017671768625368164 2.897011250060354E-4 1.9313408333735695E-4 40-41 0.024866013229684708 2.897011250060354E-4 1.9313408333735695E-4 42-43 0.036067790063251406 3.862681666747139E-4 2.897011250060354E-4 44-45 0.0509391144802279 3.862681666747139E-4 3.862681666747139E-4 46-47 0.07068707450147264 3.862681666747139E-4 3.862681666747139E-4 48-49 0.09502196900197962 3.862681666747139E-4 3.862681666747139E-4 50-51 0.12766162908599293 3.862681666747139E-4 3.862681666747139E-4 52-53 0.16643329631596737 3.862681666747139E-4 3.862681666747139E-4 54-55 0.21032301675438175 3.862681666747139E-4 3.862681666747139E-4 56-57 0.2694703297764473 3.862681666747139E-4 3.862681666747139E-4 58-59 0.33682584134035054 3.862681666747139E-4 3.862681666747139E-4 60-61 0.4183767080295495 3.862681666747139E-4 3.862681666747139E-4 62-63 0.5086668919897639 3.862681666747139E-4 3.862681666747139E-4 64-65 0.6159528752836656 3.862681666747139E-4 3.862681666747139E-4 66-67 0.7407174931195983 3.862681666747139E-4 4.828352083433924E-4 68-69 0.8768770218724349 3.862681666747139E-4 4.828352083433924E-4 70-71 1.033895031625706 3.862681666747139E-4 4.828352083433924E-4 72-73 1.2096470474627008 3.862681666747139E-4 4.828352083433924E-4 74-75 1.4044227705084253 3.862681666747139E-4 4.828352083433924E-4 76-77 1.6213123460962775 4.828352083433924E-4 4.828352083433924E-4 78-79 1.869344792622278 4.828352083433924E-4 4.828352083433924E-4 80-81 2.1374631838153637 4.828352083433924E-4 4.828352083433924E-4 82-83 2.4226739413838057 4.828352083433924E-4 4.828352083433924E-4 84-85 2.728550045869345 4.828352083433924E-4 4.828352083433924E-4 86-87 3.0735358022306984 4.828352083433924E-4 4.828352083433924E-4 88-89 3.4441601081550868 4.828352083433924E-4 4.828352083433924E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACACGTC 2910 0.0 6.4413457 94-95 CACGTCT 2785 0.0 6.3896723 94-95 CAGTCAC 1230 5.456968E-12 6.172874 94-95 CGTCTGA 2395 0.0 6.1422668 94-95 CACACGT 3065 0.0 5.9609194 92-93 ACGTCTG 2575 0.0 5.897192 94-95 GCACACG 3310 0.0 5.5913877 92-93 TCACACT 1320 2.0190782E-10 5.572246 94-95 TCCAGTC 1675 0.0 5.524625 92-93 CTCCAGT 1645 0.0 5.481005 94-95 AGTCACA 1280 3.0613592E-9 5.375775 92-93 CCAGTCA 1350 2.077286E-9 5.2726626 94-95 GTCACAC 1095 5.041966E-7 5.2023187 90-91 GTCTGAA 2630 0.0 5.1423497 94-95 AGCACAC 3640 0.0 5.0191693 94-95 GAACTCC 2135 0.0 5.0010033 94-95 >>END_MODULE