##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename NYCLib7_ATCACG_L007_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2040276 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.034040002431045 33.0 22.0 33.0 2.0 33.0 2 25.05574686954118 33.0 15.0 33.0 2.0 33.0 3 25.951428140114377 33.0 22.0 33.0 2.0 33.0 4 28.121420827378255 33.0 22.0 37.0 2.0 37.0 5 28.184067253646074 33.0 22.0 37.0 6.0 37.0 6 29.27163971933209 33.0 27.0 37.0 15.0 37.0 7 28.937363866457282 33.0 27.0 37.0 6.0 37.0 8 28.698169757424974 33.0 27.0 37.0 2.0 37.0 9 29.485322083874927 33.0 27.0 37.0 15.0 37.0 10-11 29.111658667748873 33.0 27.0 37.0 10.5 37.0 12-13 29.415570736508197 33.0 27.0 37.0 10.5 37.0 14-15 29.799233290005862 33.0 27.0 37.0 6.0 40.0 16-17 30.719288468815 33.0 27.0 37.0 15.0 40.0 18-19 31.055983112088754 33.0 27.0 37.0 15.0 40.0 20-21 31.092294375858952 33.0 27.0 37.0 15.0 40.0 22-23 31.146258643438436 33.0 27.0 37.0 15.0 40.0 24-25 30.739178669944657 33.0 27.0 37.0 10.5 40.0 26-27 30.23191739745015 33.0 27.0 37.0 15.0 40.0 28-29 30.463299573195 33.0 27.0 37.0 15.0 40.0 30-31 30.3975065138246 33.0 27.0 37.0 15.0 40.0 32-33 30.256402320078266 33.0 27.0 37.0 15.0 40.0 34-35 30.315187994173336 33.0 27.0 37.0 15.0 40.0 36-37 30.547051477349143 33.0 27.0 37.0 15.0 40.0 38-39 30.632075023183138 33.0 27.0 37.0 15.0 40.0 40-41 30.74417823863046 33.0 27.0 37.0 15.0 40.0 42-43 30.676955225665548 33.0 27.0 37.0 15.0 40.0 44-45 30.442596981976948 33.0 27.0 37.0 15.0 40.0 46-47 30.077058937124193 33.0 27.0 37.0 15.0 40.0 48-49 29.938723486430266 33.0 27.0 37.0 15.0 40.0 50-51 29.173233915411444 33.0 24.5 37.0 15.0 38.5 52-53 28.873439426822646 33.0 22.0 37.0 10.5 38.5 54-55 29.838893365407426 33.0 27.0 37.0 15.0 40.0 56-57 29.769992638250905 33.0 27.0 37.0 15.0 40.0 58-59 29.980178907167463 33.0 27.0 37.0 15.0 40.0 60-61 29.736576080883175 33.0 27.0 37.0 15.0 40.0 62-63 29.624695874479727 33.0 27.0 37.0 15.0 40.0 64-65 29.58460399475365 33.0 27.0 37.0 15.0 40.0 66-67 29.44006129562863 33.0 27.0 37.0 15.0 40.0 68-69 29.137169922108576 33.0 27.0 37.0 15.0 40.0 70-71 29.022662130025545 33.0 27.0 37.0 15.0 38.5 72-73 28.75866622947091 33.0 27.0 37.0 15.0 37.0 74-75 28.687129584428774 33.0 27.0 37.0 15.0 37.0 76-77 28.243928272449413 33.0 24.5 37.0 15.0 37.0 78-79 27.618985617632124 33.0 22.0 37.0 10.5 37.0 80-81 27.457363611589805 33.0 22.0 35.0 10.5 37.0 82-83 26.979585360019918 27.0 22.0 33.0 6.0 37.0 84-85 27.027573230288453 27.0 22.0 33.0 6.0 37.0 86-87 26.662923545637945 27.0 22.0 33.0 6.0 37.0 88-89 26.247574837914087 27.0 22.0 33.0 6.0 37.0 90-91 26.05344963132439 27.0 22.0 33.0 6.0 37.0 92-93 25.862716857915302 27.0 22.0 33.0 6.0 37.0 94-95 25.578618284977132 27.0 22.0 33.0 6.0 37.0 96-97 25.3755499255983 27.0 18.5 33.0 6.0 37.0 98-99 25.072883521641188 27.0 15.0 33.0 6.0 37.0 100-101 24.500999864724186 27.0 15.0 33.0 6.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 8.382341944828532 2306 2 8.557507116856737 2306 3 7.559978858985293 2306 4 9.12548509101995 2306 5 8.928692715934698 2306 6 7.975334106941183 2306 7 8.127652466545854 2306 8 8.635064128922412 2306 9 7.623390758960866 2306 10-11 7.90756561877761 2306 12-13 7.539242977277205 2306 14-15 8.246167985940204 2306 16-17 6.950225148037735 2306 18-19 6.365887885440145 2306 20-21 6.2616549508649335 2306 22-23 5.965237364216705 2306 24-25 5.966818289559832 2306 26-27 6.642300533945164 2306 28-29 5.785218667244575 2306 30-31 5.63226953076699 2306 32-33 5.5228807879751685 2306 34-35 4.97283181656875 2306 36-37 4.8977336749939795 2306 38-39 4.7289863357731505 2306 40-41 3.9973892196726517 2306 42-43 3.7544294857842573 2306 44-45 3.4192058512460157 2306 46-47 3.670684529104399 2306 48-49 3.6022876704984803 2306 50-51 3.530940966670638 2306 52-53 4.0681539399400535 2306 54-55 3.667114145397207 2306 56-57 3.9280399922655 2306 58-59 3.514804116515979 2306 60-61 3.573269134607468 2306 62-63 2.9351686981952803 2306 64-65 2.5182321840590234 2306 66-67 2.373953005607646 2306 68-69 2.495855605002813 2306 70-71 2.6582012580278516 2306 72-73 1.9389819175445062 2306 74-75 1.4954698867036704 2306 76-77 1.6979757700780027 2306 78-79 2.019345922529901 2306 80-81 1.6857055692374026 2306 82-83 1.6134527410081105 2306 84-85 1.3116341160130922 2306 86-87 1.3919702488162322 2306 88-89 1.2812742499319896 2306 90-91 1.208783443675145 2306 92-93 1.0938690844763634 2306 94-95 1.242174119062316 2306 96-97 0.9004417561988625 2306 98-99 0.7091032193153133 2306 100-101 0.5077114156444935 2307 1 8.326139010469763 2307 2 8.460736661542182 2307 3 7.574074624213175 2307 4 9.200354959255758 2307 5 8.935434550676792 2307 6 7.689373063829677 2307 7 8.209299138956077 2307 8 8.61293412354998 2307 9 7.525760181061983 2307 10-11 7.7455653327495995 2307 12-13 7.368423651318501 2307 14-15 8.25157337679444 2307 16-17 6.856685732364927 2307 18-19 6.4798483780313845 2307 20-21 6.324560150667946 2307 22-23 6.038154458853825 2307 24-25 6.12812674012963 2307 26-27 6.98782996644616 2307 28-29 6.111057303752428 2307 30-31 5.9458571833479255 2307 32-33 6.049950918256947 2307 34-35 5.727440986379783 2307 36-37 5.690965824875995 2307 38-39 5.221022295849245 2307 40-41 4.657715076238187 2307 42-43 4.547409139638106 2307 44-45 4.465324756515653 2307 46-47 4.909760292757866 2307 48-49 4.525884661851887 2307 50-51 4.235932353945131 2307 52-53 4.944478766553875 2307 54-55 4.647150336930167 2307 56-57 4.894475165471867 2307 58-59 4.4272062896597255 2307 60-61 4.387027139026888 2307 62-63 4.082441965857161 2307 64-65 3.9325425696040703 2307 66-67 3.529112246157304 2307 68-69 3.677361934470067 2307 70-71 3.3713309312690853 2307 72-73 2.833099285413631 2307 74-75 2.6654772579881936 2307 76-77 2.7852762522170913 2307 78-79 3.25121866264994 2307 80-81 2.8170269997914055 2307 82-83 2.8367838183164835 2307 84-85 2.1870002888319213 2307 86-87 2.151408859272127 2307 88-89 2.4468485530758812 2307 90-91 2.303534348933912 2307 92-93 2.0192493339291637 2307 94-95 2.104423644622777 2307 96-97 1.9599035123417998 2307 98-99 1.8124925675112245 2307 100-101 1.4407937217703548 1101 1 8.140756705659289 1101 2 8.043004307947825 1101 3 7.366680639850017 1101 4 8.82076783175464 1101 5 8.803608688245301 1101 6 7.5141966956649355 1101 7 7.237522772746779 1101 8 8.21358170664455 1101 9 7.389851858018879 1101 10-11 7.567964194426743 1101 12-13 7.309345271878193 1101 14-15 7.871875020318711 1101 16-17 6.968827208959244 1101 18-19 6.147086876052896 1101 20-21 5.991354081976407 1101 22-23 5.429571530494954 1101 24-25 6.071057707683643 1101 26-27 6.740981015339958 1101 28-29 5.984370644342373 1101 30-31 5.758555943696841 1101 32-33 5.838241727794376 1101 34-35 5.64967957733834 1101 36-37 5.459396474026661 1101 38-39 5.014229984760959 1101 40-41 4.540592873065368 1101 42-43 4.449456408164991 1101 44-45 4.297186810669043 1101 46-47 4.6199692485141455 1101 48-49 4.406910943477737 1101 50-51 3.9337959410760703 1101 52-53 4.784141649017965 1101 54-55 4.556695018530583 1101 56-57 4.613865950867151 1101 58-59 4.252080875700454 1101 60-61 4.517396119460376 1101 62-63 4.068435119459952 1101 64-65 3.6869181310538757 1101 66-67 3.229538075045504 1101 68-69 3.549326318205676 1101 70-71 3.533692061585395 1101 72-73 3.124607301044673 1101 74-75 2.523042713862605 1101 76-77 2.892202081051572 1101 78-79 3.309854570489499 1101 80-81 2.7597687894492218 1101 82-83 2.9111065034295933 1101 84-85 2.343286264998998 1101 86-87 2.3992223678470985 1101 88-89 2.340853759913429 1101 90-91 2.147522886198491 1101 92-93 2.001519204377086 1101 94-95 2.248079612356758 1101 96-97 2.1521396547049996 1101 98-99 1.9614779905794038 1101 100-101 1.5204930828255705 2201 1 7.144340375221763 2201 2 7.779671371721957 2201 3 6.3685830444073055 2201 4 8.480143997661369 2201 5 3.884575189500012 2201 6 5.66973255666948 2201 7 6.687345010160527 2201 8 7.6987897716056075 2201 9 6.442986215592569 2201 10-11 4.173719797949769 2201 12-13 6.492314154399224 2201 14-15 7.093767247472869 2201 16-17 5.9311978832779495 2201 18-19 1.9008535252076086 2201 20-21 5.09528635781626 2201 22-23 5.177004792714769 2201 24-25 5.53260529857473 2201 26-27 6.247521836582589 2201 28-29 5.05781233022422 2201 30-31 5.195049287534168 2201 32-33 5.343860135724263 2201 34-35 4.8389161305451935 2201 36-37 4.943454623920466 2201 38-39 4.303311363851037 2201 40-41 4.0110447108485054 2201 42-43 3.9936999059808755 2201 44-45 3.9120981006251974 2201 46-47 4.311689419977618 2201 48-49 4.028825162644903 2201 50-51 3.7929534805006497 2201 52-53 4.587108852977927 2201 54-55 4.23842072670071 2201 56-57 4.426568066771317 2201 58-59 4.05472400497516 2201 60-61 3.97564907034263 2201 62-63 3.771034444971061 2201 64-65 3.4724410242993535 2201 66-67 3.0446463228827696 2201 68-69 3.5057209492915113 2201 70-71 3.2947728841948383 2201 72-73 2.726283460382426 2201 74-75 2.328143800510869 2201 76-77 2.5556738617413686 2201 78-79 2.7779348973243145 2201 80-81 2.460182698432508 2201 82-83 2.710193983341604 2201 84-85 2.292494364315946 2201 86-87 2.1691466518064004 2201 88-89 2.1156216680949704 2201 90-91 1.972027020119036 2201 92-93 1.8853797177548657 2201 94-95 1.9501741766341674 2201 96-97 1.9507906875316863 2201 98-99 1.7791235907744642 2201 100-101 1.4947094887930383 2304 1 1.7585446213249476 2304 2 5.073201864866473 2304 3 5.861132864613715 2304 4 4.31578521574945 2304 5 6.557354385503718 2304 6 6.2075721603100575 2304 7 6.979062854789099 2304 8 6.405739802076045 2304 9 5.78858628227205 2304 10-11 6.652001635102465 2304 12-13 6.491118004766115 2304 14-15 6.869185668503434 2304 16-17 5.555128438628234 2304 18-19 5.356503285937734 2304 20-21 5.17736289355123 2304 22-23 4.629833058314773 2304 24-25 4.8441821563231 2304 26-27 5.735906486349574 2304 28-29 5.131524373152583 2304 30-31 5.0669867150778245 2304 32-33 5.234033461084827 2304 34-35 4.7450604418126545 2304 36-37 4.83786654969375 2304 38-39 4.52791750824219 2304 40-41 3.923615642671539 2304 42-43 3.2772152630975313 2304 44-45 3.269197713876249 2304 46-47 4.336556883203052 2304 48-49 4.030454402659988 2304 50-51 3.630757487580283 2304 52-53 4.353696104762317 2304 54-55 4.112652824266846 2304 56-57 4.708460508303951 2304 58-59 3.9824833908635817 2304 60-61 3.9940703072761927 2304 62-63 3.684770769720341 2304 64-65 3.614995331523172 2304 66-67 3.2870677571385762 2304 68-69 3.648089218382605 2304 70-71 3.4559642578838954 2304 72-73 2.9429943847519517 2304 74-75 2.526191328810036 2304 76-77 2.745376536256721 2304 78-79 2.9586679641261533 2304 80-81 2.8633052631104654 2304 82-83 3.106642375917545 2304 84-85 2.4789967768533927 2304 86-87 2.513081196208365 2304 88-89 2.6481763108465017 2304 90-91 2.523008858083916 2304 92-93 2.4531708616074432 2304 94-95 2.677957448900127 2304 96-97 2.601920290835409 2304 98-99 2.3987038695740317 2304 100-101 1.908177125081533 1102 1 7.135393193234261 1102 2 7.455150914201997 1102 3 6.238579238479726 1102 4 6.2661148036478345 1102 5 6.65204110095814 1102 6 5.8577144393994445 1102 7 6.789060490265548 1102 8 6.304154459453624 1102 9 4.830844691340552 1102 10-11 6.006666825239066 1102 12-13 6.001783970229248 1102 14-15 6.264595120223049 1102 16-17 5.42212228419594 1102 18-19 5.361937168596583 1102 20-21 5.449090307718659 1102 22-23 5.142092482868314 1102 24-25 1.7888816950557 1102 26-27 5.469088349342119 1102 28-29 4.854832719624223 1102 30-31 4.759738190310742 1102 32-33 4.83697940540808 1102 34-35 4.852429898246491 1102 36-37 4.76476122610611 1102 38-39 3.9123975984350494 1102 40-41 3.8919001919555463 1102 42-43 4.1086418194395335 1102 44-45 -4.016016453400077 1102 46-47 1.4918579003929153 1102 48-49 -0.6228681235406377 1102 50-51 2.6439500094595374 1102 52-53 -0.3671293066759027 1102 54-55 2.5945410186232607 1102 56-57 -0.7950279792830415 1102 58-59 2.90620589957118 1102 60-61 3.8790808216230666 1102 62-63 3.5671280514522117 1102 64-65 3.207680837290315 1102 66-67 3.01306758369077 1102 68-69 3.29785146887534 1102 70-71 3.2125143957897286 1102 72-73 2.7000780229161023 1102 74-75 2.4216419929150135 1102 76-77 2.635830124513859 1102 78-79 -1.6847271951975173 1102 80-81 1.496686554021725 1102 82-83 2.5108223569272354 1102 84-85 2.2751635061539908 1102 86-87 2.2362020856250133 1102 88-89 2.08750219185389 1102 90-91 1.999154669875253 1102 92-93 1.8921787920267086 1102 94-95 2.282472614094342 1102 96-97 2.141892376115397 1102 98-99 2.233985377591541 1102 100-101 1.9836393375491674 2202 1 -20.83983030612717 2202 2 -7.872689680911284 2202 3 -0.6515321693243372 2202 4 1.6690667785752815 2202 5 1.1181626842908727 2202 6 1.7766099419021408 2202 7 4.165040916527751 2202 8 2.926442977756217 2202 9 3.268871611227855 2202 10-11 2.184920111453394 2202 12-13 1.6036762474956063 2202 14-15 2.8815408861906846 2202 16-17 1.8200836597316474 2202 18-19 1.8107161691331903 2202 20-21 1.3175055035160135 2202 22-23 1.466493478988916 2202 24-25 2.3839561122059933 2202 26-27 3.706576063774744 2202 28-29 -0.8180362692445904 2202 30-31 1.2430122250390099 2202 32-33 1.6558402004332322 2202 34-35 1.4923097096105522 2202 36-37 3.0648311208880727 2202 38-39 2.4343997000767814 2202 40-41 1.9326600058147072 2202 42-43 0.825772918538977 2202 44-45 0.7979226773615444 2202 46-47 -1.4298597438810319 2202 48-49 0.7640700291738618 2202 50-51 1.7998952644115533 2202 52-53 1.817838916789075 2202 54-55 -0.7045105235803106 2202 56-57 1.1944845246055529 2202 58-59 2.0418346447199305 2202 60-61 2.0772702348827607 2202 62-63 -2.042576847020417 2202 64-65 0.23181840845463952 2202 66-67 0.4681259186435156 2202 68-69 1.4832442591566064 2202 70-71 -2.5131157168656237 2202 72-73 -4.5311028870115955 2202 74-75 -1.1687091683859911 2202 76-77 -0.04323610838930847 2202 78-79 1.750159537669262 2202 80-81 1.8462875379867114 2202 82-83 -0.4480619975976552 2202 84-85 0.019851182840241677 2202 86-87 0.8200529522323414 2202 88-89 1.2271182183915172 2202 90-91 1.4651906041101803 2202 92-93 1.4414264634668505 2202 94-95 1.660778001510561 2202 96-97 1.197200780842973 2202 98-99 1.1244813266531217 2202 100-101 1.026511669211903 2305 1 5.788117807080379 2305 2 7.313335524072116 2305 3 6.800941735166393 2305 4 7.886037572871192 2305 5 8.105169941412125 2305 6 6.299564243009172 2305 7 3.1379249207337523 2305 8 6.265564975369667 2305 9 6.49272404385507 2305 10-11 5.032380358998914 2305 12-13 5.416805190620103 2305 14-15 5.903476177597923 2305 16-17 5.401822359800168 2305 18-19 4.577333134463231 2305 20-21 5.346991989084469 2305 22-23 5.20596555812314 2305 24-25 5.379698793058061 2305 26-27 6.202865631626029 2305 28-29 5.1770371326823685 2305 30-31 5.489686690575809 2305 32-33 5.600036497145226 2305 34-35 5.350890177409845 2305 36-37 4.678737391194424 2305 38-39 4.595069470046411 2305 40-41 4.2356558020304895 2305 42-43 4.026220895140149 2305 44-45 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1209 48-49 -20.28730355288479 1209 50-51 -22.630454618291942 1209 52-53 -25.086669536560958 1209 54-55 -22.310560025625854 1209 56-57 -25.99336769831242 1209 58-59 -26.30350035841094 1209 60-61 -26.028709727546794 1209 62-63 -26.16186300857333 1209 64-65 -26.06013525466371 1209 66-67 -13.87226034058239 1209 68-69 -25.703042528570382 1209 70-71 -25.52720463358447 1209 72-73 -15.981456046650422 1209 74-75 -17.141779237646176 1209 76-77 -14.016885674449405 1209 78-79 -14.451964818216075 1209 80-81 -24.3932240462337 1209 82-83 -16.294293712489036 1209 84-85 -13.658560481525372 1209 86-87 -13.899219745942865 1209 88-89 -10.822421931051977 1209 90-91 -14.051059438756706 1209 92-93 -17.467087979816128 1209 94-95 -22.691601459979733 1209 96-97 -18.60916314187724 1209 98-99 -9.96849377498903 1209 100-101 -5.299810440829614 1212 1 -20.83983030612717 1212 2 -20.640379098569394 1212 3 -21.561676189361908 1212 4 -23.578585068638244 1212 5 -23.760985718965234 1212 6 -24.830152738122905 1212 7 -24.425754324549267 1212 8 -24.066982194441653 1212 9 -24.99725237123927 1212 10-11 -24.7136396881709 1212 12-13 -25.01686226221009 1212 14-15 -25.386618162024718 1212 16-17 -26.453867498690396 1212 18-19 -21.08488895344235 1212 20-21 -26.866746218462698 1212 22-23 -27.1153787814995 1212 24-25 -23.23147344583039 1212 26-27 -25.836786491015484 1212 28-29 -20.629760920329396 1212 30-31 -15.615393397777131 1212 32-33 -26.052675767005717 1212 34-35 -22.154125726776883 1212 36-37 -26.509057420126332 1212 38-39 -26.810590907312076 1212 40-41 -21.309277020461025 1212 42-43 -17.74594278969709 1212 44-45 -14.649156879932509 1212 46-47 -17.699491218167285 1212 48-49 -20.73922458965904 1212 50-51 -12.90958136060624 1212 52-53 -5.971606775054681 1212 54-55 -4.683277371112599 1212 56-57 -4.50313059928871 1212 58-59 -2.9764431757052243 1212 60-61 -14.458974720573293 1212 62-63 -12.355028977889928 1212 64-65 -16.762366774886864 1212 66-67 -13.354546517208574 1212 68-69 -8.758133184079448 1212 70-71 -15.905168371380846 1212 72-73 -12.453279143224211 1212 74-75 -8.696912328250765 1212 76-77 -19.502729326583466 1212 78-79 -10.391738101645736 1212 80-81 -8.154032972314592 1212 82-83 -5.558497446211643 1212 84-85 -4.786035508471656 1212 86-87 -3.731785004047264 1212 88-89 -3.334183525027324 1212 90-91 -3.8710356185230843 1212 92-93 -3.764572144452984 1212 94-95 -3.624655853285173 1212 96-97 -13.297767338425286 1212 98-99 -10.292574842995514 1212 100-101 -6.45809051811807 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 332.0 3 788.0 4 333.0 5 2118.0 6 2802.0 7 2355.0 8 5099.0 9 9516.0 10 11802.0 11 13560.0 12 17722.0 13 22781.0 14 26451.0 15 27890.0 16 29656.0 17 32451.0 18 37215.0 19 41603.0 20 46051.0 21 52556.0 22 59978.0 23 65505.0 24 69962.0 25 73371.0 26 78140.0 27 82447.0 28 84843.0 29 85556.0 30 87916.0 31 93979.0 32 103133.0 33 119306.0 34 137516.0 35 151860.0 36 160340.0 37 148571.0 38 54605.0 39 167.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.39716348019205 28.04175528535836 17.870655927401135 20.690425307048454 2 24.716006618266125 32.51624023664779 23.084768755946186 19.682984389139897 3 18.676317609703226 28.274485455505992 30.662603685004548 22.38659324978623 4 22.181320044824236 27.046107313316735 29.127685642779326 21.644886999079702 5 21.251114081996437 28.47101439869858 28.092064274959768 22.185807244345224 6 21.187076449914862 29.02781798617661 27.451856279099207 22.33324928480932 7 22.05903320073356 29.325264882102296 28.572711293473592 20.042990623690553 8 20.699691872185475 28.156669375682952 29.2523443199804 21.891294432151174 9 21.27509042448471 28.2915005919213 28.82519075761251 21.608218225981478 10-11 21.961661284883473 28.223890921735045 27.949024015322482 21.865423778059 12-13 21.70017385564922 28.4016721898677 28.015484853854623 21.882669100628462 14-15 21.733253038107012 28.637379053548294 27.975791625405805 21.653576282938893 16-17 21.88907020848954 28.693831003131436 27.792833605312733 21.624265183066292 18-19 21.93616953375078 28.86012827286529 27.65176363792018 21.551938555463753 20-21 21.85585801278539 28.788232724552287 27.709758153728874 21.64615110893345 22-23 21.760219497073635 28.418572494361566 28.008255949627873 21.81295205893693 24-25 21.821632119806974 28.497535805952413 28.027658168913877 21.653173905326735 26-27 21.719466300516004 28.540818282083276 27.985794216245935 21.753921201154792 28-29 21.891244555153854 28.507326791075855 27.974675679674743 21.626752974095545 30-31 21.890342990301203 28.427349376272897 27.906081417688227 21.776226215737672 32-33 21.748956035432414 28.50511056116844 28.032356132053387 21.71357727134576 34-35 21.843508713282635 28.437008794102503 28.067364236382353 21.652118256232512 36-37 21.847126952474223 28.52242908402969 27.950878113350097 21.67956585014599 38-39 21.73259064557457 28.44668452789628 28.22527521423781 21.595449612291336 40-41 21.948525901354746 28.426053259757573 27.920488576123013 21.70493226276467 42-43 21.819276086973666 28.60911535761887 27.91652207859807 21.655086476809387 44-45 21.791021785282606 28.367327417753945 28.19619549486766 21.645455302095794 46-47 21.89971227178011 28.548411876458342 27.86772357606352 21.68415227569803 48-49 21.79522583676496 28.456018464481847 28.124436581488133 21.624319117265056 50-51 22.01062101167315 28.45781167315175 27.850558754863812 21.681008560311284 52-53 21.84668306151084 28.555242075406223 28.010742143298984 21.58733271978395 54-55 21.842777000980465 28.43811934871625 28.08123956195961 21.637864088343676 56-57 21.900973456545223 28.5570238072634 28.014482427115677 21.5275203090757 58-59 22.02088826957864 28.55985624119754 27.879248783535292 21.540006705688526 60-61 22.13907799434238 28.334209953682116 27.8137337187976 21.712978333177904 62-63 22.117780920031503 28.504913045772728 27.8391377620698 21.53816827212597 64-65 22.311246528676783 28.45629850140119 27.740225992543373 21.49222897737865 66-67 22.447997148590527 28.467458609824497 27.623744096845382 21.460800144739594 68-69 22.422559658074377 28.53930385239631 27.6843662336584 21.353770255870916 70-71 22.699541619512 28.394355803304993 27.4922798424023 21.4138227347807 72-73 22.645427273670446 28.52871656879171 27.561764481532524 21.264091676005318 74-75 22.83418109630054 28.437316159243693 27.45336485928868 21.275137885167084 76-77 23.020642846186064 28.5771654995424 27.259838770160194 21.142352884111343 78-79 23.141367836112785 28.502939262175058 27.2679123309983 21.08778057071385 80-81 23.34937100108133 28.46368516648063 27.108861538627938 21.078082293810105 82-83 23.447225089360277 28.531609273268778 27.11041752372882 20.91074811364213 84-85 23.68719582802215 28.39290767689242 26.936536047950476 20.983360447134952 86-87 23.763674431098284 28.529950361769117 26.88343785531443 20.822937351818165 88-89 23.98505558780774 28.459525223294268 26.840906955999433 20.714512232898556 90-91 24.372376983435903 28.401183254883012 26.50515466446237 20.721285097218715 92-93 24.443816520211932 28.46820802153295 26.48680974501312 20.601165713242008 94-95 24.822729086907376 28.369255330438296 26.28267961183216 20.525335970822166 96-97 25.041338821889088 28.382489755696295 26.173341146013986 20.40283027640064 98-99 25.196153344391227 28.42070441586569 26.092857850498735 20.290284389244352 100-101 25.930966656915434 28.288309398594397 25.548826302496096 20.231897641994077 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 139.0 1 143.0 2 162.0 3 196.5 4 219.0 5 234.5 6 287.5 7 342.0 8 417.5 9 548.5 10 717.5 11 959.0 12 1304.0 13 1745.5 14 2220.5 15 2771.0 16 3492.5 17 4404.5 18 5541.0 19 6873.5 20 8632.5 21 10768.5 22 13221.0 23 16082.0 24 19437.0 25 23330.0 26 27272.5 27 31255.5 28 35843.0 29 40469.5 30 44856.5 31 49226.5 32 53409.5 33 57538.0 34 61323.0 35 64510.0 36 67149.0 37 69105.0 38 70390.0 39 70412.0 40 69056.0 41 66913.5 42 64566.0 43 61929.0 44 58906.0 45 55878.5 46 52971.0 47 50599.0 48 48150.5 49 45427.5 50 42921.5 51 40463.0 52 38039.0 53 35883.0 54 34110.0 55 32628.5 56 31271.5 57 30120.0 58 28947.5 59 27476.5 60 25822.5 61 24176.5 62 22853.0 63 21437.0 64 19655.0 65 18187.5 66 16819.0 67 15217.0 68 13846.0 69 12532.5 70 10912.0 71 9472.0 72 8240.5 73 6926.0 74 5828.0 75 4920.0 76 4033.0 77 3229.5 78 2582.0 79 2126.5 80 1711.0 81 1350.0 82 1106.5 83 880.5 84 685.5 85 567.0 86 470.0 87 368.0 88 287.5 89 240.0 90 181.5 91 128.5 92 90.0 93 70.0 94 65.5 95 49.5 96 32.0 97 19.5 98 10.5 99 6.5 100 3.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 13.973452611313371 2 11.338809063087544 3 10.007763655505432 4 11.911819773403206 5 9.372261399928245 6 5.529546002599648 7 9.933263930958361 8 10.381634641587707 9 5.645755770297744 10-11 6.544947840390222 12-13 5.543490194463886 14-15 7.54329316229765 16-17 2.7625429108610797 18-19 3.2448060948616755 20-21 3.1692525913160767 22-23 3.196405780394417 24-25 3.622696145031359 26-27 4.477408938790634 28-29 1.7856162597609342 30-31 2.353529620502324 32-33 3.162047683744748 34-35 2.0587900852629746 36-37 3.209075634865087 38-39 1.4136812862573496 40-41 1.1867757107371748 42-43 0.5648255432108205 44-45 1.3622421672361975 46-47 1.4215233625254624 48-49 1.3183020336464282 50-51 1.5911327683117382 52-53 2.5411267887285836 54-55 1.9204264521074599 56-57 1.9092514934253992 58-59 1.6186535547151464 60-61 1.4563470824535505 62-63 0.6231999984315848 64-65 1.2789691198641753 66-67 0.5786226961450314 68-69 0.8289074615395173 70-71 1.0396142482683715 72-73 0.0835916317204143 74-75 0.106211120456252 76-77 0.4691767192281829 78-79 0.9364174258776753 80-81 0.30439509164446377 82-83 0.518116176438874 84-85 0.06562837576876854 86-87 0.09942282318666691 88-89 0.08109196990995336 90-91 0.08974276029321523 92-93 0.17561349542904978 94-95 0.4683925116013715 96-97 0.3765666997994389 98-99 0.06420699944517311 100-101 0.22724866635690466 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2040276.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.75795303060465 #Duplication Level Percentage of deduplicated Percentage of total 1 99.04829847615099 97.8180720866903 2 0.7608706143997578 1.5028404879851716 3 0.1312961003390411 0.3889960233115373 4 0.03501026115535953 0.138301669070808 5 0.012755966396740273 0.06298765651346236 6 0.006390480623343555 0.037866647114589126 7 0.0018294269436844948 0.012646932212412117 8 0.0016038810591791413 0.012671680824727206 9 0.0 0.0 >10 0.0019447929319008918 0.025616816277009118 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 9.802595335140932E-5 4.901297667570466E-5 0.0 2 9.802595335140932E-5 4.901297667570466E-5 0.0 3 1.4703893002711397E-4 4.901297667570466E-5 0.0 4 1.4703893002711397E-4 4.901297667570466E-5 0.0 5 1.4703893002711397E-4 4.901297667570466E-5 0.0 6 1.9605190670281863E-4 9.802595335140932E-5 0.0 7 2.450648833785233E-4 9.802595335140932E-5 4.901297667570466E-5 8 4.901297667570466E-4 9.802595335140932E-5 1.4703893002711397E-4 9 5.881557201084559E-4 1.4703893002711397E-4 1.4703893002711397E-4 10-11 6.861816734598652E-4 1.715454183649663E-4 1.4703893002711397E-4 12-13 9.312465568383885E-4 1.9605190670281863E-4 1.9605190670281863E-4 14-15 0.0010047660218519454 1.9605190670281863E-4 1.9605190670281863E-4 16-17 0.0010537789985276503 1.9605190670281863E-4 1.9605190670281863E-4 18-19 0.0011518049518790595 1.9605190670281863E-4 2.450648833785233E-4 20-21 0.0012743373935683211 1.9605190670281863E-4 2.9407786005422793E-4 22-23 0.0013723633469197305 1.9605190670281863E-4 2.9407786005422793E-4 24-25 0.0015194022769468444 1.9605190670281863E-4 2.9407786005422793E-4 26-27 0.0019115060903524817 1.9605190670281863E-4 2.9407786005422793E-4 28-29 0.002744726693839461 1.9605190670281863E-4 3.430908367299326E-4 30-31 0.004754258737543352 1.9605190670281863E-4 3.430908367299326E-4 32-33 0.008503751453234759 1.9605190670281863E-4 3.430908367299326E-4 34-35 0.014287282700967907 1.9605190670281863E-4 3.430908367299326E-4 36-37 0.025584773824717834 1.9605190670281863E-4 3.430908367299326E-4 38-39 0.04095034201255124 1.9605190670281863E-4 3.430908367299326E-4 40-41 0.0646726227235923 1.9605190670281863E-4 3.430908367299326E-4 42-43 0.10018252432514033 1.9605190670281863E-4 3.6759732506778497E-4 44-45 0.14757807277054674 1.9605190670281863E-4 3.9210381340563726E-4 46-47 0.212422240912504 1.9605190670281863E-4 3.9210381340563726E-4 48-49 0.30130727411389435 2.450648833785233E-4 3.9210381340563726E-4 50-51 0.4054843560381046 2.450648833785233E-4 3.9210381340563726E-4 52-53 0.5296097194693267 2.450648833785233E-4 3.9210381340563726E-4 54-55 0.6864757513199194 2.450648833785233E-4 3.9210381340563726E-4 56-57 0.8745140363362603 2.450648833785233E-4 3.9210381340563726E-4 58-59 1.1038702606902202 2.450648833785233E-4 3.9210381340563726E-4 60-61 1.3627322970029545 2.450648833785233E-4 3.9210381340563726E-4 62-63 1.6479633147672177 2.450648833785233E-4 3.9210381340563726E-4 64-65 1.9696109742015295 2.450648833785233E-4 3.9210381340563726E-4 66-67 2.3205683936879127 2.450648833785233E-4 3.9210381340563726E-4 68-69 2.716838310110985 2.450648833785233E-4 3.9210381340563726E-4 70-71 3.1401143766823703 2.450648833785233E-4 3.9210381340563726E-4 72-73 3.573658661867316 2.450648833785233E-4 3.9210381340563726E-4 74-75 4.038350693729672 2.450648833785233E-4 3.9210381340563726E-4 76-77 4.523113539540729 2.450648833785233E-4 3.9210381340563726E-4 78-79 5.055835583028962 2.450648833785233E-4 3.9210381340563726E-4 80-81 5.633208448268764 2.450648833785233E-4 4.4111679008134195E-4 82-83 6.237195359843472 2.450648833785233E-4 4.4111679008134195E-4 84-85 6.852749333913647 2.450648833785233E-4 4.4111679008134195E-4 86-87 7.505945274070763 2.450648833785233E-4 4.4111679008134195E-4 88-89 8.174482275927375 2.695713717163756E-4 4.4111679008134195E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTGTG 820 5.566615E-6 5.5232806 94-95 GTAGGGA 4965 0.0 5.3820004 94-95 >>END_MODULE