##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename NYCLib7_ATCACG_L007_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2040276 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.229657164030748 33.0 33.0 33.0 27.0 33.0 2 31.631563572771526 33.0 33.0 33.0 27.0 33.0 3 31.791930601546067 33.0 33.0 33.0 27.0 33.0 4 35.621337995447675 37.0 37.0 37.0 33.0 37.0 5 35.12693184647567 37.0 37.0 37.0 33.0 37.0 6 34.931297530334135 37.0 33.0 37.0 33.0 37.0 7 34.72978655828917 37.0 33.0 37.0 27.0 37.0 8 34.77908920165703 37.0 33.0 37.0 27.0 37.0 9 34.67509934930372 37.0 33.0 37.0 27.0 37.0 10-11 34.524616032340724 37.0 33.0 37.0 27.0 37.0 12-13 34.52735634786666 37.0 33.0 37.0 27.0 37.0 14-15 35.44907012580651 37.0 33.0 40.0 27.0 40.0 16-17 35.358965159615664 37.0 33.0 40.0 27.0 40.0 18-19 35.58114073782175 37.0 33.0 40.0 27.0 40.0 20-21 35.27232394048649 37.0 33.0 40.0 27.0 40.0 22-23 35.31039575037887 37.0 33.0 40.0 27.0 40.0 24-25 35.16189574351705 37.0 33.0 40.0 27.0 40.0 26-27 34.79109100925561 37.0 33.0 40.0 27.0 40.0 28-29 34.440983229719905 37.0 33.0 40.0 27.0 40.0 30-31 34.345340532359344 37.0 33.0 40.0 27.0 40.0 32-33 34.356763251638505 37.0 33.0 40.0 27.0 40.0 34-35 34.22922119360322 37.0 33.0 40.0 24.5 40.0 36-37 33.94408477088393 37.0 33.0 37.0 22.0 40.0 38-39 33.88105922924154 37.0 33.0 37.0 22.0 40.0 40-41 33.61196548898286 37.0 33.0 37.0 22.0 40.0 42-43 33.265442518561215 37.0 33.0 37.0 22.0 40.0 44-45 33.179017936788945 37.0 33.0 37.0 22.0 40.0 46-47 33.04463342214485 37.0 33.0 37.0 22.0 40.0 48-49 33.08429986923338 37.0 33.0 37.0 22.0 40.0 50-51 33.38055929687944 37.0 33.0 37.0 22.0 40.0 52-53 33.240384144105995 37.0 33.0 37.0 22.0 40.0 54-55 32.88654304613689 37.0 33.0 37.0 22.0 40.0 56-57 32.94340251024861 37.0 33.0 37.0 22.0 40.0 58-59 32.65906965528193 37.0 33.0 37.0 22.0 40.0 60-61 32.56111526087647 37.0 33.0 37.0 22.0 40.0 62-63 32.54709681435257 37.0 33.0 37.0 22.0 40.0 64-65 32.21315866088706 35.0 27.0 37.0 22.0 40.0 66-67 31.866670489678846 33.0 27.0 37.0 22.0 40.0 68-69 31.696599381652284 33.0 27.0 37.0 22.0 40.0 70-71 31.541838457149915 33.0 27.0 37.0 22.0 40.0 72-73 31.3097348103884 33.0 27.0 37.0 15.0 37.0 74-75 30.96072026529744 33.0 27.0 37.0 15.0 37.0 76-77 29.86472295905064 33.0 27.0 37.0 15.0 37.0 78-79 30.767313834010693 33.0 27.0 37.0 18.5 37.0 80-81 30.4700758622853 33.0 27.0 37.0 15.0 37.0 82-83 30.447024324160065 33.0 27.0 37.0 15.0 37.0 84-85 30.310685662135906 33.0 27.0 37.0 15.0 37.0 86-87 30.115414777216415 33.0 27.0 37.0 15.0 37.0 88-89 29.976695554915118 33.0 27.0 37.0 15.0 37.0 90-91 29.88533830717021 33.0 27.0 37.0 15.0 37.0 92-93 29.67459721135768 33.0 27.0 35.0 15.0 37.0 94-95 29.57913169590781 33.0 27.0 33.0 15.0 37.0 96-97 29.432929907522315 33.0 27.0 33.0 15.0 37.0 98-99 29.16886489867057 33.0 27.0 33.0 15.0 37.0 100-101 28.334559883074643 33.0 24.5 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 1.0830109328326394 2306 2 0.6437659327980256 2306 3 0.5075796645421349 2306 4 0.32920464154584295 2306 5 0.6858649960583634 2306 6 0.760173287773128 2306 7 0.8100773862182962 2306 8 0.7942718622846598 2306 9 1.0318086176914676 2306 10-11 1.2112010367868464 2306 12-13 1.3298958601346271 2306 14-15 1.4544007105008063 2306 16-17 1.515442781483138 2306 18-19 1.3110969134676367 2306 20-21 1.6071030767903238 2306 22-23 1.2560222005963766 2306 24-25 1.1991159678591643 2306 26-27 1.4031348887486033 2306 28-29 1.6467361911644147 2306 30-31 1.6595902574872738 2306 32-33 1.2443914731982133 2306 34-35 1.1398700969175124 2306 36-37 1.3360871524846303 2306 38-39 1.1906529231799396 2306 40-41 1.3371845619998908 2306 42-43 1.1842473233097053 2306 44-45 1.0209549539161955 2306 46-47 1.0438038588382241 2306 48-49 1.0487926239147853 2306 50-51 1.1264218152161405 2306 52-53 1.2771281873130746 2306 54-55 1.6124909073895424 2306 56-57 1.2994029462240562 2306 58-59 1.2534061561369398 2306 60-61 1.0205110986963675 2306 62-63 0.7381363094631723 2306 64-65 0.7979051016094623 2306 66-67 1.0585603866085727 2306 68-69 0.9269250556593889 2306 70-71 0.8200903970507447 2306 72-73 0.6863662170550029 2306 74-75 0.8653555942695164 2306 76-77 0.7519562938462201 2306 78-79 0.7937907387306176 2306 80-81 1.2853097260160702 2306 82-83 1.093557049238754 2306 84-85 0.9111701093761617 2306 86-87 0.7011077600121141 2306 88-89 0.5217525919273562 2306 90-91 0.35480418866609753 2306 92-93 0.09270335455421375 2306 94-95 0.25899343670722175 2306 96-97 0.27397491552473596 2306 98-99 0.32220466996851016 2306 100-101 0.26903752817645454 2307 1 0.5270847744741154 2307 2 0.43225428839522806 2307 3 0.29881136949170894 2307 4 0.33214599420112023 2307 5 0.5036124690856454 2307 6 0.606168796422601 2307 7 0.8063420863169952 2307 8 0.7532054650028428 2307 9 0.9253200578184178 2307 10-11 1.1754998338070095 2307 12-13 1.3655735149391859 2307 14-15 1.2319397395758216 2307 16-17 1.4105458273572822 2307 18-19 1.1464177284759955 2307 20-21 1.4840450694541616 2307 22-23 1.2308487569164441 2307 24-25 1.1795813843451057 2307 26-27 1.1775046888750396 2307 28-29 1.5279714003751863 2307 30-31 1.4815575060264763 2307 32-33 1.1615123504511118 2307 34-35 1.1843287643552785 2307 36-37 1.2476023617832155 2307 38-39 1.067004016071408 2307 40-41 1.1832230898463791 2307 42-43 1.275543154566563 2307 44-45 1.1204919147244041 2307 46-47 1.1319862867873383 2307 48-49 0.9740300945867162 2307 50-51 0.9275792981989852 2307 52-53 1.1374607189107522 2307 54-55 1.5314973636144558 2307 56-57 1.2614382696369475 2307 58-59 1.161610925842055 2307 60-61 0.8354245318405091 2307 62-63 0.8773662940630302 2307 64-65 0.975137476466724 2307 66-67 0.9553407891323467 2307 68-69 0.8304724876433198 2307 70-71 0.8307804584244529 2307 72-73 0.7142860679524503 2307 74-75 0.7861391928635015 2307 76-77 0.9220055866325723 2307 78-79 0.83856473217811 2307 80-81 1.5634824649756887 2307 82-83 1.3186644325770125 2307 84-85 1.127208727129112 2307 86-87 0.9647111044070797 2307 88-89 0.9870146426080453 2307 90-91 0.771547903080517 2307 92-93 0.7888166354428101 2307 94-95 0.6149056543368943 2307 96-97 0.5084764265282935 2307 98-99 0.5700348140791611 2307 100-101 0.6815076538792795 1101 1 0.9899763066426317 1101 2 0.5531627892542801 1101 3 0.4324945221135508 1101 4 0.3001225250755013 1101 5 0.5597028154850605 1101 6 0.7027738334808404 1101 7 0.8354141868669132 1101 8 0.695070987372894 1101 9 0.8975774403226353 1101 10-11 1.0024629782031127 1101 12-13 1.2278871936480158 1101 14-15 1.2905805091503169 1101 16-17 1.2827945285756854 1101 18-19 0.9147221336805984 1101 20-21 1.3407164495862816 1101 22-23 1.024926293476966 1101 24-25 1.1098097934876634 1101 26-27 1.2029415009865474 1101 28-29 1.3195984345009109 1101 30-31 1.4160590578600392 1101 32-33 0.8738543991292786 1101 34-35 0.9644580370816342 1101 36-37 1.3219268465440663 1101 38-39 1.0915279232903785 1101 40-41 1.0893166111467423 1101 42-43 1.1254274989957196 1101 44-45 0.9558106087023006 1101 46-47 0.8318122689088341 1101 48-49 1.006049131744021 1101 50-51 0.8924053173878548 1101 52-53 1.256887303865895 1101 54-55 1.6377618890548646 1101 56-57 1.1570149181384508 1101 58-59 1.1640858981140454 1101 60-61 1.0032273403009384 1101 62-63 0.905567096501791 1101 64-65 0.6972371883404271 1101 66-67 0.8981305514896682 1101 68-69 0.7690747114492282 1101 70-71 0.9827596626621293 1101 72-73 0.7646809590157417 1101 74-75 0.760457440636209 1101 76-77 0.8865263103552721 1101 78-79 0.9053861363180751 1101 80-81 1.471970699630841 1101 82-83 1.3790729128609875 1101 84-85 1.0787470337862928 1101 86-87 0.9298281293218338 1101 88-89 0.9021417777826493 1101 90-91 0.7754624055230437 1101 92-93 0.9496824196481732 1101 94-95 0.9003530204422958 1101 96-97 0.7550186585663887 1101 98-99 0.8274760351664732 1101 100-101 0.8563618313774093 2201 1 1.5282327864264929 2201 2 0.8574320974649723 2201 3 0.3768795418202018 2201 4 0.3027425860646318 2201 5 0.4467743589283302 2201 6 0.5372316065626066 2201 7 0.623275454589816 2201 8 0.5990946191375244 2201 9 0.568674850784312 2201 10-11 0.8864506295923817 2201 12-13 1.1122418645888104 2201 14-15 1.01734557363946 2201 16-17 0.9402365584406027 2201 18-19 0.7601716187743008 2201 20-21 1.1688954711463566 2201 22-23 0.86663668570516 2201 24-25 0.7849964300208185 2201 26-27 0.9133267572611032 2201 28-29 1.3104086572665423 2201 30-31 1.44882548714763 2201 32-33 1.0049565398884468 2201 34-35 0.8848637833592292 2201 36-37 0.8018057717114644 2201 38-39 0.6496564772100157 2201 40-41 0.9543862006516122 2201 42-43 0.9160031208493109 2201 44-45 0.7347627783538258 2201 46-47 0.8970781005470343 2201 48-49 0.8948664635285795 2201 50-51 0.9023875069323921 2201 52-53 1.0054235864193544 2201 54-55 1.2821372011010084 2201 56-57 0.9657064872076049 2201 58-59 1.1237529280094591 2201 60-61 0.7120817181297667 2201 62-63 0.6337500308163371 2201 64-65 0.7810512258296036 2201 66-67 0.8570313071231475 2201 68-69 0.7533117982858215 2201 70-71 0.8599935711014375 2201 72-73 0.7709581995143644 2201 74-75 0.8264500743722252 2201 76-77 0.8671299433955255 2201 78-79 0.6880188665729392 2201 80-81 1.2693427972272424 2201 82-83 1.070099047569066 2201 84-85 0.9930032342694695 2201 86-87 0.9586730030806265 2201 88-89 1.0985497790368512 2201 90-91 0.8213113255895514 2201 92-93 0.9699937508420042 2201 94-95 0.8419756513900722 2201 96-97 0.5999095953068512 2201 98-99 0.8967386023586208 2201 100-101 0.9510268966777318 2304 1 -1.897738216789378 2304 2 -0.2861258303957719 2304 3 -0.13170091366399106 2304 4 -0.01219397589741078 2304 5 0.37674413347367874 2304 6 0.596249399783531 2304 7 0.6462085739310339 2304 8 0.5999609996203574 2304 9 0.85236839042458 2304 10-11 0.7625315913639241 2304 12-13 1.1676572951620727 2304 14-15 1.2109037544774424 2304 16-17 1.1662343772441872 2304 18-19 0.8190595614395377 2304 20-21 1.0533497133550256 2304 22-23 0.9508799049497441 2304 24-25 0.974239917554435 2304 26-27 1.1298759759142598 2304 28-29 1.4125985481095213 2304 30-31 1.4550106475824691 2304 32-33 0.993650920345182 2304 34-35 1.0975710765733027 2304 36-37 1.2746441377607525 2304 38-39 0.9638131369624148 2304 40-41 0.8771677599953449 2304 42-43 0.8580118493630593 2304 44-45 1.0249790366153633 2304 46-47 0.9540599595489709 2304 48-49 0.9787753533752337 2304 50-51 0.8082099379451719 2304 52-53 1.0739835147975043 2304 54-55 1.4359610786110224 2304 56-57 1.0331144703971304 2304 58-59 0.8786268341658214 2304 60-61 0.4312999132649651 2304 62-63 0.2621568853279328 2304 64-65 0.2387092930009196 2304 66-67 0.5146929159758535 2304 68-69 0.4894795685324205 2304 70-71 0.45264815051546137 2304 72-73 0.23613468376180435 2304 74-75 0.42609218480424715 2304 76-77 0.4763946424051966 2304 78-79 0.4512043067473499 2304 80-81 1.0417996536228422 2304 82-83 0.7623011705274543 2304 84-85 0.5671721341260323 2304 86-87 0.41685236980402607 2304 88-89 0.2863370163882202 2304 90-91 0.15377108856052857 2304 92-93 0.3958774769091953 2304 94-95 0.1331943985482411 2304 96-97 0.11771343462262962 2304 98-99 0.47340535290721775 2304 100-101 0.31712011719386624 1102 1 1.4334699361471124 1102 2 0.15945521605785018 1102 3 0.28721505235506584 1102 4 0.162499692293963 1102 5 0.3086900325819073 1102 6 0.12626209705261715 1102 7 -4.780504982942428 1102 8 -1.1009960023713248 1102 9 0.22518593148316057 1102 10-11 0.5651825261660832 1102 12-13 0.8955146810367438 1102 14-15 0.7714458233540142 1102 16-17 0.5866307277243905 1102 18-19 0.7719260459118829 1102 20-21 1.0256675171796275 1102 22-23 0.6265193084375298 1102 24-25 0.9398618206030349 1102 26-27 0.9002869286245243 1102 28-29 0.9647452612364802 1102 30-31 1.1052393540843184 1102 32-33 0.876126242946988 1102 34-35 1.0671875790204766 1102 36-37 1.1189383348938193 1102 38-39 0.8515252491787066 1102 40-41 1.1556368829498354 1102 42-43 1.2165906224644232 1102 44-45 0.9681794377690167 1102 46-47 0.7466795329118412 1102 48-49 0.8328199116725941 1102 50-51 0.5970102498547263 1102 52-53 0.7740464822819746 1102 54-55 1.1946681956786591 1102 56-57 1.1110885258663359 1102 58-59 0.9213201228474546 1102 60-61 0.7576873660208179 1102 62-63 0.6582845518100342 1102 64-65 0.40678140578053856 1102 66-67 0.5821540329056241 1102 68-69 0.7722255883128852 1102 70-71 0.5664011534233033 1102 72-73 0.5334920507351164 1102 74-75 0.38229094265109254 1102 76-77 0.6031937001536818 1102 78-79 0.6754820708123752 1102 80-81 1.2002838457822627 1102 82-83 0.9572548770107723 1102 84-85 0.7586159314767293 1102 86-87 0.6091325710331361 1102 88-89 0.5239114080793321 1102 90-91 0.15468939834869389 1102 92-93 0.2767794648138029 1102 94-95 0.20136183349975312 1102 96-97 0.16368410860078697 1102 98-99 0.19475574045929633 1102 100-101 0.34092642194759293 2202 1 1.5965866036534777 2202 2 0.929179019709494 2202 3 0.4446688830129659 2202 4 -0.3294741635388547 2202 5 0.146594996470931 2202 6 0.514571115158887 2202 7 0.7177279746064897 2202 8 -3.5444895099138023 2202 9 -0.3064410574992138 2202 10-11 0.46497216206545033 2202 12-13 -0.10000277626465959 2202 14-15 0.08844093151898846 2202 16-17 0.12634539867111272 2202 18-19 0.6460532608127494 2202 20-21 1.1137219071557212 2202 22-23 -2.688864940925299 2202 24-25 -2.8744818042171296 2202 26-27 -1.4367985709272375 2202 28-29 0.42821991957975314 2202 30-31 1.2000328725828382 2202 32-33 1.0492095145026141 2202 34-35 -2.0545974009587518 2202 36-37 -0.2452851332788626 2202 38-39 0.21698071284741616 2202 40-41 1.2592510589551296 2202 42-43 1.2216970600617287 2202 44-45 1.3260488384892355 2202 46-47 0.6866775416302886 2202 48-49 0.9104310695668261 2202 50-51 -3.96030199188845 2202 52-53 -0.37422102280218894 2202 54-55 1.5275685652253301 2202 56-57 1.2840249304115758 2202 58-59 1.3363848874803779 2202 60-61 1.3278454529830412 2202 62-63 1.274679276593325 2202 64-65 1.3133941798916346 2202 66-67 1.2235431397158827 2202 68-69 1.1011968554796923 2202 70-71 1.2841075649500233 2202 72-73 1.1156006259611786 2202 74-75 0.6155184741577244 2202 76-77 1.3008016032222542 2202 78-79 1.2047898165777013 2202 80-81 1.8569742718765276 2202 82-83 1.6637008313104609 2202 84-85 1.53491908130027 2202 86-87 1.540954129814729 2202 88-89 1.3660983470317554 2202 90-91 1.250760655838988 2202 92-93 1.3770019445431174 2202 94-95 1.1065164936983578 2202 96-97 1.086161238852192 2202 98-99 1.183252530817338 2202 100-101 1.0375041367081081 2305 1 1.269622388615847 2305 2 0.5890858246267321 2305 3 0.13351925546839283 2305 4 -0.07145481564598555 2305 5 0.32325085031245493 2305 6 0.32675728970786366 2305 7 0.483247381572383 2305 8 -0.09156369677359777 2305 9 0.4147481046680568 2305 10-11 0.3465167149417141 2305 12-13 0.8182802145435772 2305 14-15 0.7338897979076222 2305 16-17 0.7172637700868592 2305 18-19 0.7078578605339203 2305 20-21 0.78925112762807 2305 22-23 0.5006496338670559 2305 24-25 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-5.1277544850448855 1208 36-37 -2.5895065545076115 1208 38-39 -0.20704200339493184 1208 40-41 -0.5045755136082306 1208 42-43 0.4357415853879516 1208 44-45 0.09185058639169341 1208 46-47 0.02590475859373953 1208 48-49 -1.3663751526842347 1208 50-51 -0.001347633558090422 1208 52-53 -1.3107195843968036 1208 54-55 -0.6394359270731442 1208 56-57 -5.850633908273693 1208 58-59 -2.105930465382869 1208 60-61 -1.4449919509420468 1208 62-63 0.26068753076891227 1208 64-65 0.04745659985078632 1208 66-67 0.9432826043543123 1208 68-69 0.5525656706299245 1208 70-71 -4.074162730994896 1208 72-73 -0.8204113907819064 1208 74-75 -5.548792330486322 1208 76-77 -1.1877993706268626 1208 78-79 -4.037783772683863 1208 80-81 0.767525788329305 1208 82-83 0.23672208325819355 1208 84-85 -2.696759458740697 1208 86-87 -0.8064946423110655 1208 88-89 -4.805040999827948 1208 90-91 -0.10076463379240863 1208 92-93 -2.189955705516013 1208 94-95 -4.978265543183092 1208 96-97 -3.544602652098245 1208 98-99 -6.233615266368538 1208 100-101 -3.8123939287798976 1209 1 1.7613491338031864 1209 2 1.14100431079153 1209 3 0.5007076524602425 1209 4 0.27074099647847305 1209 5 0.45740913708194597 1209 6 0.9950473122881363 1209 7 0.4787092008153664 1209 8 0.28204058391069964 1209 9 -0.34509428788427954 1209 10-11 -2.8531106148451144 1209 12-13 -0.4714224386387116 1209 14-15 -1.2616395947212595 1209 16-17 -0.5906286817865407 1209 18-19 -16.808721538792497 1209 20-21 -14.366436757480429 1209 22-23 -3.934430282126666 1209 24-25 -2.2592441819512388 1209 26-27 -1.9837863941911849 1209 28-29 -3.636329038215102 1209 30-31 -1.1044068032291818 1209 32-33 -0.6364272842110026 1209 34-35 -3.6625397141956633 1209 36-37 -4.223041982137204 1209 38-39 -1.7590674936655617 1209 40-41 -9.873233975738472 1209 42-43 -6.1095982438867225 1209 44-45 -3.471351777015748 1209 46-47 -3.3899217742777026 1209 48-49 -3.431457753416428 1209 50-51 -3.515708554141966 1209 52-53 -1.6013948820032056 1209 54-55 -3.4447578429360206 1209 56-57 -1.5902487140104569 1209 58-59 -2.691075640055402 1209 60-61 -1.9546933717148924 1209 62-63 -0.929995817809079 1209 64-65 -0.1240878001393746 1209 66-67 0.3406545212235592 1209 68-69 -1.3670051231287843 1209 70-71 -1.4031578579206965 1209 72-73 -0.6695730833644973 1209 74-75 0.5133930386619099 1209 76-77 -17.181871283694264 1209 78-79 -16.945966355301366 1209 80-81 -22.09821363750224 1209 82-83 -10.143766834355766 1209 84-85 -3.6714945035018616 1209 86-87 -2.314862620913374 1209 88-89 -1.8985211664830608 1209 90-91 -6.355798168325485 1209 92-93 -3.720906659444328 1209 94-95 -1.3636370396127084 1209 96-97 -0.29824963041205876 1209 98-99 0.9103143010482846 1209 100-101 0.038980662801023414 1212 1 -28.595648837799246 1212 2 -16.264165981246798 1212 3 -6.01150941154037 1212 4 -1.7103923600527153 1212 5 -0.9385074751263502 1212 6 -2.6460394787982295 1212 7 -0.606489154966134 1212 8 -1.2228521826029208 1212 9 -0.04446143915973266 1212 10-11 0.5545095967079874 1212 12-13 0.03828388221022294 1212 14-15 -0.292351287853279 1212 16-17 -0.13083027678032977 1212 18-19 -1.173782178544002 1212 20-21 -1.2588652048368658 1212 22-23 -0.8305180492202311 1212 24-25 -0.05472823908585411 1212 26-27 -0.8599197648674064 1212 28-29 0.1010769326857357 1212 30-31 -0.8137702485063016 1212 32-33 0.05830454013033659 1212 34-35 -1.7806875071463466 1212 36-37 -1.7193897349159961 1212 38-39 -0.4114943567784337 1212 40-41 -0.4653124750071669 1212 42-43 0.4175701187692624 1212 44-45 1.0925979119349947 1212 46-47 -1.8667352380848108 1212 48-49 -0.8512837129446638 1212 50-51 -0.22769731732866916 1212 52-53 -1.2850171983370409 1212 54-55 -0.38993452852873745 1212 56-57 -1.7346100799678936 1212 58-59 -0.26656201697523585 1212 60-61 0.20409026870707692 1212 62-63 -4.492461692919768 1212 64-65 -4.231626819209705 1212 66-67 -1.5194383833455696 1212 68-69 0.17414984701105496 1212 70-71 -0.905969890250585 1212 72-73 -1.0973131882074725 1212 74-75 -5.879108092088359E-4 1212 76-77 1.2079191152239233 1212 78-79 -1.1744524691236116 1212 80-81 0.45957730458517076 1212 82-83 0.4544152461520987 1212 84-85 -3.4486862054603833 1212 86-87 -1.7856375706277845 1212 88-89 -1.1595450970479284 1212 90-91 -0.3385171829259903 1212 92-93 -2.6435404077928943 1212 94-95 -1.830352365188908 1212 96-97 -0.21514049583339911 1212 98-99 -0.5682729497516199 1212 100-101 -0.022234791494966544 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 3.0 10 4.0 11 17.0 12 42.0 13 163.0 14 555.0 15 1249.0 16 2266.0 17 3797.0 18 5987.0 19 8763.0 20 12352.0 21 17042.0 22 22349.0 23 29385.0 24 37162.0 25 46597.0 26 56637.0 27 69017.0 28 83209.0 29 99260.0 30 117360.0 31 136114.0 32 158686.0 33 181306.0 34 202989.0 35 222557.0 36 235039.0 37 214802.0 38 75369.0 39 197.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 31.910787025793212 27.595858237025894 20.29386850850329 20.199486228677603 2 24.153436469320205 31.65718974286584 24.47124548884213 19.718128298971827 3 18.55704462789502 27.037584713011164 32.61919617541003 21.786174483683784 4 22.002132065530382 26.431682003651467 30.58548479984316 20.980701130974992 5 20.807331949206873 28.428310679535514 28.923978912656917 21.8403784586007 6 20.639364478139232 29.152624448849078 28.22789661790856 21.980114455103134 7 21.338681629348187 29.405531408495712 29.436115505941352 19.819671456214746 8 20.21834300849493 28.148152504857187 29.836845603241912 21.79665888340597 9 21.0055894398601 28.149328816297402 29.185022026431717 21.660059717410782 10-11 21.44087368571703 28.198709390298177 28.448626558367597 21.9117903656172 12-13 21.424893298264163 28.31556260256713 28.39519754401224 21.864346555156466 14-15 21.32907508591975 28.577457167559682 28.367902185782707 21.725565560737863 16-17 21.427134294202656 28.562536193117534 28.311393023621136 21.69893648905868 18-19 21.709893860974514 28.716669308405784 27.993119046579178 21.580317784040524 20-21 21.454523799721215 28.57530059658595 28.184691678968925 21.785483924723913 22-23 21.40414895050912 28.29290169217385 28.395804487140214 21.907144870176815 24-25 21.54152183332059 28.204174337197518 28.422625174241134 21.83167865524076 26-27 21.663882729591485 28.356849759542335 28.159229437585896 21.820038073280283 28-29 21.76426130582333 28.314281989299488 28.262965402720024 21.65849130215716 30-31 21.7472783094052 28.239708745287402 28.164963955856955 21.848048989450447 32-33 21.634009320307644 28.30161213482882 28.249707392529245 21.81467115233429 34-35 21.734118325167774 28.24340922502642 28.262254714558228 21.760217735247583 36-37 21.72956011833693 28.307224120658187 28.207139622286398 21.756076138718488 38-39 21.700201345308184 28.131757664159164 28.43649584664036 21.731545143892298 40-41 21.832785276626904 28.1846881664354 28.156186603628303 21.826339953309393 42-43 21.562450374361116 28.318644144223626 28.371112535754968 21.747792945660294 44-45 21.612713181942052 28.080661635974742 28.44597985762711 21.8606453244561 46-47 21.74583510230975 28.280344114529658 28.271862832669704 21.701957950490886 48-49 21.756694132728644 28.155731909734737 28.36727196890812 21.720301988628496 50-51 21.751479579958584 28.194900197277327 28.218426437612575 21.83519378515151 52-53 21.68887078674953 28.247547082512668 28.381891750350256 21.681690380387543 54-55 21.571774926190866 28.12927571593084 28.507860761674493 21.79108859620381 56-57 21.74767902729782 28.350311198921506 28.18778245557111 21.714227318209566 58-59 21.786392381774743 28.24240655065325 28.301549843740037 21.669651223831977 60-61 21.93847417643631 28.17793318364572 28.11791599946869 21.76567664044928 62-63 21.839470595717057 28.289496094974382 28.130422880859772 21.74061042844879 64-65 21.9209276587527 28.282308932362138 28.08813993303706 21.708623475848103 66-67 21.97748739088026 28.368093167793194 27.96018998230557 21.69422945902097 68-69 21.95599013615962 28.396135829258117 27.881573136474792 21.76630089810747 70-71 22.10264835742238 28.421832479825586 27.682594127657573 21.79292503509446 72-73 21.96184262719165 28.499740867986826 27.81700033488584 21.721416169935683 74-75 21.918514179884088 28.384506019092516 27.852139774456568 21.844840026566825 76-77 21.963947276629305 28.58080541166415 27.682666125330062 21.772581186376485 78-79 22.095400958597207 28.59852578551988 27.50291260042161 21.803160655461305 80-81 21.980139132073397 28.522590314146402 27.509353334223192 21.98791721955701 82-83 21.992518839530504 28.640816315527246 27.388948537428963 21.97771630751329 84-85 21.93512400471624 28.517371835733485 27.432469955535883 22.115034204014393 86-87 21.905003015695588 28.64408395520056 27.293037627695288 22.157875401408567 88-89 21.934640164066206 28.707052219638534 27.210913696626527 22.14739391966873 90-91 22.018163322834067 28.63684384689863 26.982143075999165 22.362849754268137 92-93 21.83494099363431 28.651597133734935 27.065725231382253 22.447736641248504 94-95 21.934299967794104 28.650798358234493 26.857488655631695 22.557413018339716 96-97 21.923537215628425 28.6642920988064 26.82584424563518 22.586326439929994 98-99 21.811736338621362 28.605716971924934 26.89132145632812 22.691225233125582 100-101 21.86925178507923 28.526537896031712 26.70402059179853 22.900189727090535 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1.0 1 2.5 2 4.0 3 7.0 4 16.0 5 22.0 6 29.0 7 46.5 8 71.5 9 108.0 10 133.5 11 195.0 12 294.0 13 418.0 14 636.0 15 908.5 16 1253.5 17 1850.0 18 2672.5 19 3728.0 20 5127.5 21 6909.5 22 9223.0 23 12107.5 24 15437.5 25 19181.0 26 23320.0 27 27763.0 28 32409.5 29 37573.5 30 42525.5 31 47024.0 32 51947.0 33 56542.5 34 60778.5 35 64877.5 36 68120.5 37 70835.0 38 72788.0 39 73030.0 40 72285.5 41 70705.0 42 68241.0 43 65245.0 44 61825.0 45 58962.0 46 56486.5 47 53675.0 48 50832.0 49 47976.5 50 45226.5 51 42469.0 52 39844.5 53 37735.0 54 35989.5 55 34420.0 56 33217.5 57 32077.0 58 30579.5 59 29207.0 60 27732.0 61 26113.0 62 24561.5 63 23011.0 64 21398.0 65 19806.0 66 18276.0 67 16711.0 68 15032.0 69 13397.5 70 11849.5 71 10194.0 72 8564.5 73 7009.0 74 5495.5 75 4226.5 76 3182.0 77 2234.5 78 1525.0 79 1023.0 80 656.5 81 422.5 82 275.5 83 188.5 84 135.0 85 103.5 86 70.5 87 47.5 88 38.5 89 29.5 90 16.5 91 12.0 92 8.5 93 5.5 94 4.5 95 1.5 96 0.0 97 0.0 98 0.0 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7093157984507978 2 0.0027937396705151655 3 2.450648833785233E-4 4 4.901297667570466E-5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.2014188276488083 14-15 0.0 16-17 2.6957137171637564E-4 18-19 0.12140514322572045 20-21 0.0 22-23 4.901297667570466E-5 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0018134801370010725 42-43 0.0 44-45 0.0 46-47 0.024016358571095284 48-49 2.450648833785233E-5 50-51 4.901297667570466E-5 52-53 7.351946501355698E-5 54-55 0.0023281163920959714 56-57 0.0010537789985276503 58-59 0.09832003121146354 60-61 0.0013233503702440258 62-63 0.0010047660218519454 64-65 0.0021075579970553007 66-67 0.004215115994110601 68-69 0.004754258737543352 70-71 0.0033328824139479173 72-73 0.03798505692367111 74-75 0.043646055729715 76-77 0.1638258745385428 78-79 1.4703893002711397E-4 80-81 0.12253244168926164 82-83 0.004141596529097044 84-85 0.004484687365826977 86-87 0.005710011782719593 88-89 0.006077609107787378 90-91 0.0221048524807428 92-93 0.00977808884680308 94-95 0.013576594539170192 96-97 0.009091907173343215 98-99 0.01115045219372281 100-101 0.01178762089050697 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2040276.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.05790461660122 #Duplication Level Percentage of deduplicated Percentage of total 1 98.6867194858106 96.77012926264896 2 0.9831890022891683 1.9281890681312517 3 0.17058364215396493 0.501812235344578 4 0.08652751261237036 0.33938826313822285 5 0.03596331516010141 0.1763243663832996 6 0.02573844854958494 0.15143149997126987 7 0.0036841257701126784 0.02528803573528901 8 0.003229922222446736 0.025337552416617816 9 0.0014383300396921086 0.012693566685538891 >10 0.0028699876677798727 0.038530141782236224 >50 0.0 0.0 >100 0.0 0.0 >500 5.622772418332114E-5 0.030876007762749333 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 9.802595335140932E-5 0.0 0.0 2 1.4703893002711397E-4 0.0 0.0 3 1.4703893002711397E-4 0.0 0.0 4 1.9605190670281863E-4 0.0 0.0 5 2.450648833785233E-4 0.0 0.0 6 2.450648833785233E-4 0.0 0.0 7 2.450648833785233E-4 0.0 0.0 8 5.391427434327513E-4 0.0 0.0 9 6.371686967841606E-4 0.0 0.0 10-11 8.087141151491269E-4 0.0 0.0 12-13 0.0010782854868655026 2.450648833785233E-5 0.0 14-15 0.0012008179285547642 4.901297667570466E-5 0.0 16-17 0.0012498309052304687 4.901297667570466E-5 0.0 18-19 0.001396869835257583 4.901297667570466E-5 0.0 20-21 0.0015194022769468444 4.901297667570466E-5 0.0 22-23 0.0017399606719875156 4.901297667570466E-5 4.901297667570466E-5 24-25 0.0019360125786903341 4.901297667570466E-5 4.901297667570466E-5 26-27 0.0025486747871366422 4.901297667570466E-5 7.351946501355698E-5 28-29 0.003724986227353554 4.901297667570466E-5 9.802595335140932E-5 30-31 0.006249154526152344 9.802595335140932E-5 9.802595335140932E-5 32-33 0.010586802961952206 9.802595335140932E-5 9.802595335140932E-5 34-35 0.0173751002315373 9.802595335140932E-5 9.802595335140932E-5 36-37 0.029089201657030713 9.802595335140932E-5 1.4703893002711397E-4 38-39 0.04643979540023016 1.4703893002711397E-4 1.4703893002711397E-4 40-41 0.07371551692025981 1.4703893002711397E-4 1.4703893002711397E-4 42-43 0.11111241812382247 1.4703893002711397E-4 1.4703893002711397E-4 44-45 0.1617918360065011 1.4703893002711397E-4 1.4703893002711397E-4 46-47 0.23131674342098812 1.4703893002711397E-4 1.4703893002711397E-4 48-49 0.3249560353599219 1.4703893002711397E-4 1.4703893002711397E-4 50-51 0.44023455650117926 1.4703893002711397E-4 1.4703893002711397E-4 52-53 0.5741625152675423 1.4703893002711397E-4 1.715454183649663E-4 54-55 0.7365425069941518 1.715454183649663E-4 1.9605190670281863E-4 56-57 0.9402404380583803 1.9605190670281863E-4 2.450648833785233E-4 58-59 1.1768995959370203 1.9605190670281863E-4 2.450648833785233E-4 60-61 1.4460543573516524 1.9605190670281863E-4 2.450648833785233E-4 62-63 1.7389313994773257 1.9605190670281863E-4 2.450648833785233E-4 64-65 2.0644265775806803 2.450648833785233E-4 2.695713717163756E-4 66-67 2.423250579823514 2.9407786005422793E-4 2.9407786005422793E-4 68-69 2.8289800007449974 2.9407786005422793E-4 2.9407786005422793E-4 70-71 3.2664453240639992 2.9407786005422793E-4 2.9407786005422793E-4 72-73 3.732656758203302 3.185843483920803E-4 3.430908367299326E-4 74-75 4.223766784493863 3.430908367299326E-4 3.430908367299326E-4 76-77 4.750803322687715 3.430908367299326E-4 3.430908367299326E-4 78-79 5.326632279162231 3.430908367299326E-4 3.6759732506778497E-4 80-81 5.933780527732523 3.430908367299326E-4 3.9210381340563726E-4 82-83 6.583202468685609 3.430908367299326E-4 3.9210381340563726E-4 84-85 7.2442159786225 3.430908367299326E-4 3.9210381340563726E-4 86-87 7.941376558857723 3.430908367299326E-4 3.9210381340563726E-4 88-89 8.663754315592596 3.430908367299326E-4 3.9210381340563726E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGCCG 1190 0.0 9.172665 94-95 CGTATGC 1440 0.0 8.404122 94-95 CTCGTAT 1540 0.0 8.320659 94-95 GTATGCC 1550 0.0 8.266977 94-95 TCGTATG 1615 0.0 7.9347367 92-93 TCTCGTA 1755 0.0 7.4365287 94-95 ACGATCT 2790 0.0 7.2293525 94-95 GGACAAC 745 0.0016807142 7.055908 1 CATCACG 4560 0.0 6.97308 94-95 CGATCTC 3065 0.0 6.8903966 94-95 GCCGTCT 1125 0.0 6.749664 94-95 GCCGCGA 850 9.052939E-4 6.746505 1 TCACGAT 3425 0.0 6.720823 92-93 GATCTCG 2860 0.0 6.720532 94-95 TCACATC 6140 0.0 6.608645 94-95 CACGATC 3220 0.0 6.5591183 92-93 ATCGTAT 560 7.9215344E-5 6.3564487 92-93 ATCTCGT 2155 0.0 6.2764235 94-95 ATCACGA 4160 0.0 6.103446 94-95 ATGCCGT 1385 0.0 5.9969387 92-93 >>END_MODULE