FastQCFastQC Report
Thu 11 Sep 2014
NYCLib7_ATCACG_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNYCLib7_ATCACG_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2040276
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG11900.09.17266594-95
CGTATGC14400.08.40412294-95
CTCGTAT15400.08.32065994-95
GTATGCC15500.08.26697794-95
TCGTATG16150.07.934736792-93
TCTCGTA17550.07.436528794-95
ACGATCT27900.07.229352594-95
GGACAAC7450.00168071427.0559081
CATCACG45600.06.9730894-95
CGATCTC30650.06.890396694-95
GCCGTCT11250.06.74966494-95
GCCGCGA8509.052939E-46.7465051
TCACGAT34250.06.72082392-93
GATCTCG28600.06.72053294-95
TCACATC61400.06.60864594-95
CACGATC32200.06.559118392-93
ATCGTAT5607.9215344E-56.356448792-93
ATCTCGT21550.06.276423594-95
ATCACGA41600.06.10344694-95
ATGCCGT13850.05.996938792-93
Produced by FastQC (version 0.11.2)