##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename NYCLib6_CTTGTA_L007_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1615365 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.97934213010682 33.0 22.0 33.0 2.0 33.0 2 24.978529929768193 33.0 15.0 33.0 2.0 33.0 3 25.88094455432673 33.0 22.0 33.0 2.0 33.0 4 28.046798711127206 33.0 22.0 37.0 2.0 37.0 5 28.12633739123975 33.0 22.0 37.0 6.0 37.0 6 29.196705388565434 33.0 27.0 37.0 15.0 37.0 7 28.835710195528566 33.0 27.0 37.0 2.0 37.0 8 28.572066994146834 33.0 27.0 37.0 2.0 37.0 9 29.366821120923134 33.0 27.0 37.0 15.0 37.0 10-11 29.001290729958864 33.0 27.0 37.0 10.5 37.0 12-13 29.312175885945283 33.0 27.0 37.0 10.5 37.0 14-15 29.68822216650726 33.0 27.0 37.0 6.0 40.0 16-17 30.609907667926443 33.0 27.0 37.0 15.0 40.0 18-19 30.946779829945555 33.0 27.0 37.0 15.0 40.0 20-21 30.96666357139099 33.0 27.0 37.0 15.0 40.0 22-23 31.02504139931223 33.0 27.0 37.0 15.0 40.0 24-25 30.602839296381934 33.0 27.0 37.0 10.5 40.0 26-27 30.078008066288426 33.0 27.0 37.0 15.0 40.0 28-29 30.314946776734672 33.0 27.0 37.0 15.0 40.0 30-31 30.242889996997583 33.0 27.0 37.0 15.0 40.0 32-33 30.08441250119942 33.0 27.0 37.0 15.0 40.0 34-35 30.139561647058095 33.0 27.0 37.0 15.0 40.0 36-37 30.368942313347137 33.0 27.0 37.0 15.0 40.0 38-39 30.444770376973626 33.0 27.0 37.0 15.0 40.0 40-41 30.53775214889514 33.0 27.0 37.0 15.0 40.0 42-43 30.46606834987758 33.0 27.0 37.0 15.0 40.0 44-45 30.24268942313347 33.0 27.0 37.0 15.0 40.0 46-47 29.85616377722682 33.0 27.0 37.0 15.0 40.0 48-49 29.712621915170875 33.0 27.0 37.0 15.0 40.0 50-51 28.92841370216638 33.0 24.5 37.0 15.0 38.5 52-53 28.637672600310147 33.0 22.0 37.0 10.5 38.5 54-55 29.613270994481123 33.0 27.0 37.0 15.0 40.0 56-57 29.545330931399405 33.0 27.0 37.0 15.0 40.0 58-59 29.7445147072024 33.0 27.0 37.0 15.0 40.0 60-61 29.50515611022896 33.0 27.0 37.0 10.5 40.0 62-63 29.397239633147926 33.0 27.0 37.0 15.0 40.0 64-65 29.37065926276724 33.0 27.0 37.0 15.0 40.0 66-67 29.249225716788466 33.0 27.0 37.0 15.0 40.0 68-69 28.970835074425906 33.0 27.0 37.0 15.0 40.0 70-71 28.86275485726136 33.0 27.0 37.0 15.0 38.5 72-73 28.625647763818083 33.0 22.0 37.0 15.0 37.0 74-75 28.5756980620479 33.0 27.0 37.0 15.0 37.0 76-77 28.166934098485484 33.0 22.0 37.0 15.0 37.0 78-79 27.563321911766074 33.0 22.0 37.0 10.5 37.0 80-81 27.43618841562124 30.0 22.0 35.0 10.5 37.0 82-83 26.9933086330334 27.0 22.0 33.0 6.0 37.0 84-85 27.08219597428445 27.0 22.0 33.0 6.0 37.0 86-87 26.761107861071643 27.0 22.0 33.0 6.0 37.0 88-89 26.367276435975768 27.0 22.0 33.0 6.0 37.0 90-91 26.21386931127021 27.0 22.0 33.0 6.0 37.0 92-93 26.077673466987335 27.0 22.0 33.0 6.0 37.0 94-95 25.83201598400362 27.0 22.0 33.0 6.0 37.0 96-97 25.661957514246005 27.0 22.0 33.0 6.0 37.0 98-99 25.409718237054783 27.0 22.0 33.0 6.0 37.0 100-101 24.8822584988532 27.0 18.5 33.0 6.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 8.356022595154567 2306 2 8.45848501292317 2306 3 7.5071088433912685 2306 4 9.024166311220075 2306 5 8.88880820600426 2306 6 7.899065131782255 2306 7 8.141685670542198 2306 8 8.564831216385802 2306 9 7.406203341290677 2306 10-11 7.822532249195458 2306 12-13 7.519305863339696 2306 14-15 8.168834930705525 2306 16-17 6.9240461424423465 2306 18-19 6.305141139839979 2306 20-21 6.190346323797957 2306 22-23 5.8736314436789385 2306 24-25 5.875808961855569 2306 26-27 6.574140335990496 2306 28-29 5.587828147108539 2306 30-31 5.4384938594600065 2306 32-33 5.459879423552792 2306 34-35 4.847532608153195 2306 36-37 4.926911886196422 2306 38-39 4.515329602133598 2306 40-41 3.8885641984349135 2306 42-43 3.700987169875525 2306 44-45 3.4692239289684146 2306 46-47 3.762371604822615 2306 48-49 3.5963070323963464 2306 50-51 3.3930343013219577 2306 52-53 4.037178396049779 2306 54-55 3.5426255242318945 2306 56-57 3.8273173568018564 2306 58-59 3.289916863768788 2306 60-61 3.312649277189564 2306 62-63 2.7940397592566555 2306 64-65 2.4873793441072074 2306 66-67 2.469454864979987 2306 68-69 2.624430364740981 2306 70-71 2.65491627115264 2306 72-73 1.9571001032656064 2306 74-75 1.5477296278330641 2306 76-77 1.9411274614679819 2306 78-79 2.2489613907863557 2306 80-81 2.00366450692648 2306 82-83 1.8067427669405696 2306 84-85 1.299564875068274 2306 86-87 1.2809557703578598 2306 88-89 1.2130182251621697 2306 90-91 1.1705796550231007 2306 92-93 1.2808447772212723 2306 94-95 1.532810962071121 2306 96-97 1.1637144229411867 2306 98-99 0.9807718780104473 2306 100-101 0.756522611065801 2307 1 8.383352379216053 2307 2 8.507387870293634 2307 3 7.609210257062031 2307 4 9.247503254980952 2307 5 9.1139661984916 2307 6 7.542933369655945 2307 7 8.278987847463608 2307 8 8.640062979365641 2307 9 7.622391190200361 2307 10-11 7.847308754304475 2307 12-13 7.427793293646133 2307 14-15 8.418477070749024 2307 16-17 7.015389298219265 2307 18-19 6.611592378678171 2307 20-21 6.482532522883364 2307 22-23 6.111167515264206 2307 24-25 6.306876077269379 2307 26-27 7.059668594016255 2307 28-29 6.2332456386083095 2307 30-31 6.217794513639131 2307 32-33 6.154787030892891 2307 34-35 5.744076960845895 2307 36-37 5.773545433376391 2307 38-39 5.252515508516595 2307 40-41 4.736290357607608 2307 42-43 4.304016652811313 2307 44-45 4.588100553477599 2307 46-47 4.832532761858456 2307 48-49 4.525089258436591 2307 50-51 4.249862557252673 2307 52-53 4.897825621011027 2307 54-55 4.548356758235105 2307 56-57 4.962371760223036 2307 58-59 4.420149178098249 2307 60-61 4.408082507371525 2307 62-63 4.098262235163883 2307 64-65 3.8986733551916224 2307 66-67 3.5490628523848997 2307 68-69 3.786705489320564 2307 70-71 3.507420635608618 2307 72-73 2.9105553324265934 2307 74-75 2.496042347128167 2307 76-77 2.6834188480592474 2307 78-79 3.015485218099343 2307 80-81 2.830066411397553 2307 82-83 2.960279711373925 2307 84-85 2.178163250367259 2307 86-87 2.1028470831829402 2307 88-89 2.3357900765183146 2307 90-91 2.177008201880188 2307 92-93 2.1121225694018655 2307 94-95 2.2009894072583016 2307 96-97 1.9426205108151962 2307 98-99 1.5275146001739515 2307 100-101 1.30191209107371 1101 1 8.103167195677038 1101 2 7.956683322608839 1101 3 7.236855720628313 1101 4 8.66789641704339 1101 5 8.494956004193899 1101 6 7.299760056137064 1101 7 7.0451240836627385 1101 8 8.102071977789446 1101 9 7.237260010973884 1101 10-11 7.516912031288289 1101 12-13 7.267039936667224 1101 14-15 7.773943788381079 1101 16-17 6.8995397336125315 1101 18-19 6.031608485762657 1101 20-21 5.754893935461251 1101 22-23 5.501006543770487 1101 24-25 6.08704444083596 1101 26-27 6.640306897154684 1101 28-29 6.092071078145565 1101 30-31 5.75864451835395 1101 32-33 5.919856723969346 1101 34-35 5.789233662855423 1101 36-37 5.540129708323157 1101 38-39 5.23611729906802 1101 40-41 4.464533026060508 1101 42-43 4.389592707236218 1101 44-45 4.214571974252831 1101 46-47 4.555276737301636 1101 48-49 4.508804823464775 1101 50-51 4.04085566245838 1101 52-53 4.917299488787219 1101 54-55 4.6896971217940155 1101 56-57 4.852366046661409 1101 58-59 4.415723346594632 1101 60-61 4.588193735321887 1101 62-63 4.221899534158677 1101 64-65 3.770549263186897 1101 66-67 3.50468174283494 1101 68-69 3.767812346343998 1101 70-71 3.576386313626937 1101 72-73 3.0536464546708437 1101 74-75 2.389327378381072 1101 76-77 2.8128375114658013 1101 78-79 3.3374779682554774 1101 80-81 2.9382390094139836 1101 82-83 2.994031687720689 1101 84-85 2.308431496682072 1101 86-87 2.328127344884283 1101 88-89 2.533024210708927 1101 90-91 2.337539021037248 1101 92-93 2.0644391676469773 1101 94-95 2.237146596088376 1101 96-97 2.0897247216125017 1101 98-99 2.024733944989837 1101 100-101 1.805736630350264 2201 1 7.129979414984561 2201 2 7.875425889524333 2201 3 6.324266372227598 2201 4 8.467056333595604 2201 5 3.957207959330855 2201 6 5.797807254861194 2201 7 6.700377408027062 2201 8 7.763854997639957 2201 9 6.581925197338634 2201 10-11 4.228969865486857 2201 12-13 6.62387929138292 2201 14-15 7.181232614385383 2201 16-17 6.087711176657759 2201 18-19 2.1686440271957075 2201 20-21 5.082483745807519 2201 22-23 5.247370755482031 2201 24-25 5.514625911940485 2201 26-27 6.254190315939294 2201 28-29 5.197129952846211 2201 30-31 5.345660353570533 2201 32-33 5.4263196363163075 2201 34-35 5.188777807375516 2201 36-37 5.204451093010988 2201 38-39 4.664257603176985 2201 40-41 4.337573174492086 2201 42-43 4.202942397248265 2201 44-45 4.01663444866848 2201 46-47 4.464372514358072 2201 48-49 4.208481046348833 2201 50-51 3.8158766749253736 2201 52-53 4.723579249239904 2201 54-55 4.400814715137297 2201 56-57 4.498371895895538 2201 58-59 4.145863580806214 2201 60-61 4.006400739523688 2201 62-63 3.8975770802485563 2201 64-65 3.7568413318067435 2201 66-67 3.381909938053475 2201 68-69 3.6432790065053666 2201 70-71 3.5338207995403614 2201 72-73 2.887833176175807 2201 74-75 2.459041363151055 2201 76-77 2.7300530628713418 2201 78-79 3.051608328943253 2201 80-81 2.708174555033956 2201 82-83 2.8998629630606523 2201 84-85 2.467135571595456 2201 86-87 2.3280762194499722 2201 88-89 2.425507076335368 2201 90-91 2.0510249936367835 2201 92-93 2.0498613253237004 2201 94-95 2.380619270884594 2201 96-97 2.11216627858008 2201 98-99 2.1653444605314505 2201 100-101 1.748453479182647 2304 1 1.4910070087591976 2304 2 4.660022855139495 2304 3 5.483343925424734 2304 4 4.183699468821494 2304 5 6.441626285105546 2304 6 6.121953800552408 2304 7 6.801156899984289 2304 8 6.217818066850324 2304 9 5.545503963133726 2304 10-11 6.492643526982622 2304 12-13 6.3297914127858235 2304 14-15 6.563148650222505 2304 16-17 5.3252952887560845 2304 18-19 5.014622546582135 2304 20-21 4.89565551186023 2304 22-23 4.407061107362132 2304 24-25 4.638256864462122 2304 26-27 5.562358490501833 2304 28-29 4.6910423608807506 2304 30-31 4.797420774639722 2304 32-33 4.9667760596046335 2304 34-35 4.74215881452691 2304 36-37 4.777466904807021 2304 38-39 4.302131451735349 2304 40-41 3.631896116819604 2304 42-43 3.0641738991400516 2304 44-45 2.854980863420174 2304 46-47 3.908799709813561 2304 48-49 3.721141183515183 2304 50-51 3.2785338254501717 2304 52-53 3.949312088352105 2304 54-55 3.7393693890486794 2304 56-57 4.396855090235441 2304 58-59 3.6599220678423663 2304 60-61 3.6746931025093588 2304 62-63 3.3346856609986446 2304 64-65 3.339398818747707 2304 66-67 3.1410034558165805 2304 68-69 3.275893518961915 2304 70-71 3.206565568244315 2304 72-73 2.9234484468955273 2304 74-75 2.295192918664881 2304 76-77 2.4310374405873887 2304 78-79 2.8049946218055872 2304 80-81 2.434434622325785 2304 82-83 2.759741613021319 2304 84-85 2.2175202359861146 2304 86-87 2.063763438149703 2304 88-89 2.330356275646853 2304 90-91 2.3841953329772743 2304 92-93 2.068553887533401 2304 94-95 2.3177991335291637 2304 96-97 2.261948769426237 2304 98-99 2.196995179463343 2304 100-101 1.7375053429651501 1102 1 7.068943136297339 1102 2 7.519059541705143 1102 3 6.235836831461917 1102 4 6.264150319029916 1102 5 6.501848017981747 1102 6 5.831774676509195 1102 7 6.738991233958057 1102 8 6.327927206928759 1102 9 4.797100845821422 1102 10-11 5.994522247370693 1102 12-13 5.7556351947845386 1102 14-15 6.270110065135508 1102 16-17 5.400475465688981 1102 18-19 5.314841056762194 1102 20-21 5.416490692888484 1102 22-23 5.316585217824482 1102 24-25 1.8639074269755511 1102 26-27 5.436251237087884 1102 28-29 4.804149153774027 1102 30-31 4.983499284639567 1102 32-33 5.016169628345764 1102 34-35 4.733244882093025 1102 36-37 4.720851259814857 1102 38-39 3.836465852344695 1102 40-41 3.805919039935418 1102 42-43 3.9688871455831105 1102 44-45 -3.631953166875544 1102 46-47 1.4739023215016793 1102 48-49 -0.8644039098054677 1102 50-51 2.635071499692746 1102 52-53 -0.49278745667624335 1102 54-55 2.4288022063870223 1102 56-57 -1.069325274984486 1102 58-59 2.7410187022472883 1102 60-61 3.636572448939944 1102 62-63 3.2175235235756716 1102 64-65 2.9088593621810652 1102 66-67 2.8638030349452706 1102 68-69 3.2149535564390987 1102 70-71 3.125966270075917 1102 72-73 2.5823233346702956 1102 74-75 2.357895048584826 1102 76-77 2.72510449021501 1102 78-79 -1.630338328471936 1102 80-81 1.4716787570899754 1102 82-83 2.5388499221705985 1102 84-85 2.2420633553757447 1102 86-87 2.2024184569475764 1102 88-89 2.1006654861076406 1102 90-91 2.0681775491013816 1102 92-93 2.119270623214934 1102 94-95 2.4559055944302024 1102 96-97 2.2922943425231033 1102 98-99 2.209930130800391 1102 100-101 1.6596849977216728 2202 1 -20.853481896297186 2202 2 -7.831943742966947 2202 3 -0.6222764920179102 2202 4 1.972674137360599 2202 5 1.6133375941026316 2202 6 2.1082731712255445 2202 7 4.144158746120816 2202 8 2.9004125120079003 2202 9 3.648875241275153 2202 10-11 2.6098374449528663 2202 12-13 1.759952026805685 2202 14-15 3.001109393629715 2202 16-17 1.8708520871996832 2202 18-19 1.8930639024284623 2202 20-21 1.35954319363616 2202 22-23 1.6547047494211746 2202 24-25 2.7155570614430573 2202 26-27 3.733701553047151 2202 28-29 -0.7833742126403891 2202 30-31 1.2351623186535257 2202 32-33 1.9248715893158916 2202 34-35 1.861768759014879 2202 36-37 3.297901135678 2202 38-39 2.828634250507889 2202 40-41 2.0314246817558654 2202 42-43 1.081928623547988 2202 44-45 1.0722092391938922 2202 46-47 -0.9202431720983775 2202 48-49 1.1318949749752463 2202 50-51 2.0564809307316985 2202 52-53 2.030542593003979 2202 54-55 -0.6893604585025521 2202 56-57 1.2315712845928033 2202 58-59 2.168485059450667 2202 60-61 2.1992628384082025 2202 62-63 -2.215970082089431 2202 64-65 0.2442782228324063 2202 66-67 0.8358510333434381 2202 68-69 1.664141062457432 2202 70-71 -2.5211537445158356 2202 72-73 -4.389145311664723 2202 74-75 -1.1060938613799003 2202 76-77 -0.07889561830602787 2202 78-79 1.8485777017162732 2202 80-81 1.928434714156399 2202 82-83 -0.6339519408249004 2202 84-85 -0.019220794998755508 2202 86-87 0.779440502003677 2202 88-89 1.4278655336938648 2202 90-91 1.5076291127978827 2202 92-93 1.4695808125255603 2202 94-95 1.852645084829657 2202 96-97 1.4146839784069876 2202 98-99 1.5695011644454837 2202 100-101 1.1454558130000088 2305 1 5.744513649359828 2305 2 7.116149845233483 2305 3 6.829550806890438 2305 4 7.637669772661486 2305 5 7.8885991061597345 2305 6 6.096652975767096 2305 7 2.829554889345612 2305 8 6.017678878759252 2305 9 6.374683798522341 2305 10-11 4.885562407115319 2305 12-13 5.29906612778646 2305 14-15 5.624372978810104 2305 16-17 4.977635560699479 2305 18-19 4.512275671711254 2305 20-21 5.233838553652038 2305 22-23 5.070078542601458 2305 24-25 5.141655481709186 2305 26-27 6.158133076009474 2305 28-29 5.153704493807268 2305 30-31 5.169499924431111 2305 32-33 5.214533562299138 2305 34-35 5.168524425508352 2305 36-37 4.6486299490455565 2305 38-39 4.549377058763135 2305 40-41 4.008095952462071 2305 42-43 3.820569961904866 2305 44-45 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-26.384592025713168 1209 38-39 -26.681135190680074 1209 40-41 -23.16374270133649 1209 42-43 -23.88548052064896 1209 44-45 -26.831268681869023 1209 46-47 -20.230597332433 1209 48-49 -20.04966032006188 1209 50-51 -22.091160653799296 1209 52-53 -24.942102971669723 1209 54-55 -21.83992274411759 1209 56-57 -25.82176986429348 1209 58-59 -26.129092119134718 1209 60-61 -25.895696129938806 1209 62-63 -26.022535146567744 1209 64-65 -25.911055892056222 1209 66-67 -13.599757494293225 1209 68-69 -25.618977343169767 1209 70-71 -25.436941161475584 1209 72-73 -15.366439683019276 1209 74-75 -16.37635857705298 1209 76-77 -13.581183838530944 1209 78-79 -14.167803118337854 1209 80-81 -24.4189666144876 1209 82-83 -15.930712828324356 1209 84-85 -13.169845315572477 1209 86-87 -12.93681005883461 1209 88-89 -10.36380366579089 1209 90-91 -13.013318016433734 1209 92-93 -17.573873661878505 1209 94-95 -22.96994186319692 1209 96-97 -18.56545872780059 1209 98-99 -10.067278248809117 1209 100-101 -5.04716427876037 1212 1 -20.853481896297186 1212 2 -20.645542805100593 1212 3 -21.583589504913572 1212 4 -23.616227777449875 1212 5 -23.797369713439377 1212 6 -24.856947618145302 1212 7 -24.40801006982291 1212 8 -24.033546065561463 1212 9 -24.97832288299214 1212 10-11 -24.680510347153426 1212 12-13 -24.974319172881692 1212 14-15 -25.353912099140896 1212 16-17 -26.409335486071125 1212 18-19 -21.123667872421912 1212 20-21 -26.86007384426927 1212 22-23 -27.041465629633144 1212 24-25 -23.088874472770552 1212 26-27 -25.77430938872698 1212 28-29 -20.734611525221375 1212 30-31 -15.33231270499881 1212 32-33 -25.96883689055906 1212 34-35 -22.185081174655757 1212 36-37 -26.384592025713168 1212 38-39 -26.681135190680074 1212 40-41 -21.122478081453448 1212 42-43 -17.280710783806857 1212 44-45 -13.952979208184814 1212 46-47 -17.302604317488232 1212 48-49 -20.578662513044335 1212 50-51 -12.544580081999428 1212 52-53 -5.060524024301301 1212 54-55 -3.959342822090303 1212 56-57 -4.061079074819794 1212 58-59 -2.736822382292612 1212 60-61 -14.402275077307227 1212 62-63 -12.263489093936165 1212 64-65 -16.559904576266746 1212 66-67 -12.712726548874123 1212 68-69 -7.693812869485555 1212 70-71 -15.34154642463348 1212 72-73 -12.084170352285035 1212 74-75 -8.208495922731991 1212 76-77 -19.387975992527046 1212 78-79 -9.87196775446521 1212 80-81 -7.804657403961286 1212 82-83 -5.024998890702523 1212 84-85 -4.237866351959742 1212 86-87 -3.3078423850204466 1212 88-89 -2.9558013103652883 1212 90-91 -3.2522759436592033 1212 92-93 -3.3015600653872745 1212 94-95 -3.0497116000390214 1212 96-97 -12.949758597846074 1212 98-99 -10.3281314814277 1212 100-101 -6.313028882399099 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 278.0 3 617.0 4 281.0 5 1744.0 6 2315.0 7 1971.0 8 4141.0 9 8152.0 10 10080.0 11 11534.0 12 15033.0 13 19160.0 14 21689.0 15 23347.0 16 24590.0 17 26746.0 18 30740.0 19 34226.0 20 36744.0 21 41968.0 22 47676.0 23 51490.0 24 54875.0 25 57219.0 26 60785.0 27 63854.0 28 65709.0 29 66320.0 30 67486.0 31 72393.0 32 78945.0 33 92451.0 34 106561.0 35 118915.0 36 127849.0 37 120535.0 38 46805.0 39 141.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.00898274390384 26.48633411770134 16.12359275151683 22.381090386877986 2 23.526489150949008 30.783425552774318 25.26481453360662 20.425270762670056 3 19.129269963245278 27.681530716448393 30.461012015914648 22.728187304391685 4 22.0597554243742 27.379500597075456 28.352353378545843 22.208390600004506 5 20.93611845880968 28.280077624530662 28.22638071179533 22.557423204864325 6 20.961764102106685 28.65164007269536 27.93813155079723 22.448464274400727 7 22.21458397336195 28.506886133914882 28.536505395527357 20.742024497195814 8 21.0360274310141 28.053250719136646 28.969786570763322 21.940935279085927 9 21.52737646181165 27.805942861946143 28.880367856429867 21.78631281981234 10-11 22.05537149243683 27.97759404630431 27.86549794877407 22.101536512484785 12-13 21.76863676327981 28.160871856541924 28.04235320198773 22.028138178190535 14-15 21.825663453336983 28.386569193878042 27.934342662181955 21.85342469060302 16-17 22.0525046442448 28.36393302097855 27.763604277863717 21.819958056912935 18-19 22.217325866550947 28.260164818372406 27.639127706236582 21.883381608840065 20-21 22.06447971582672 28.35525627460272 27.689329135920104 21.890934873650462 22-23 21.953476195627335 28.158698378093284 27.87216515169651 22.015660274582874 24-25 21.92729621346495 28.20277756355878 28.01390067093391 21.856025552042365 26-27 21.84680930008089 28.28016743414886 27.96011471042494 21.912908555345314 28-29 22.09301849026779 28.25725025244381 27.84098199460087 21.808749262687527 30-31 22.11549589901822 28.138788118425108 27.80407621766674 21.94163976488994 32-33 21.86932739085884 28.277720709668593 27.924172046917235 21.928779852555337 34-35 22.027764010653698 28.212216202641667 27.931546098815236 21.828473687889407 36-37 22.063113453221778 28.216071870338443 27.84719584129562 21.873618835144164 38-39 22.00916405914801 28.250027797478566 27.91997592769766 21.820832215675768 40-41 22.16489595245475 28.179474298292924 27.72624750971279 21.929382239539542 42-43 22.02027850879105 28.22454693437321 27.856141154960834 21.89903340187491 44-45 21.875100028808298 28.180821959077157 28.038945804627026 21.905132207487522 46-47 22.11082022870956 28.28193381635094 27.75756045648963 21.849685498449865 48-49 22.031795563828428 28.209473924921163 27.896072967748026 21.86265754350238 50-51 22.132754677012162 28.182622877671896 27.76316836992049 21.921454075395452 52-53 22.01234603201835 28.22914184593194 27.900761539854557 21.857750582195155 54-55 21.979365946333285 28.156866011806663 27.968208020441576 21.895560021418472 56-57 21.946097117086012 28.25118744700236 28.03144939697479 21.771266038936837 58-59 22.1627705186272 28.169029155883372 27.81011585371419 21.858084471775232 60-61 22.15430907061627 28.04936754044624 27.754221843483855 22.042101545453633 62-63 22.03992616074836 28.239120783256872 27.82842499630025 21.892528059694516 64-65 22.26093724716416 28.15683379599025 27.752421892933704 21.829807063911883 66-67 22.354531088224075 28.177335606467537 27.672357286874206 21.795776018434186 68-69 22.26571585655176 28.24306912563538 27.71865828000611 21.772556737806752 70-71 22.48053453910832 28.1797549194756 27.502757985015837 21.836952556400245 72-73 22.40686807390887 28.19251152676909 27.628867001391917 21.771753397930123 74-75 22.509562397899867 28.187569254566775 27.56235699784087 21.74051134969249 76-77 22.662561482892407 28.287418020218208 27.371620734765273 21.678399762124112 78-79 22.728694348602172 28.224744897082427 27.383894936247316 21.66266581806809 80-81 22.85207884772671 28.153638922820967 27.314604069075287 21.679678160377037 82-83 22.871149779627412 28.13598110212116 27.335845210617833 21.6570239076336 84-85 23.046187774116014 28.07682062591372 27.23387516415987 21.64311643581039 86-87 23.02648355138305 28.217941269878153 27.250218824313123 21.505356354425672 88-89 23.243428277574438 28.200344791800585 27.109309533508775 21.446917397116202 90-91 23.46671326227503 28.098143239361395 26.844120340594067 21.591023157769506 92-93 23.51582774928515 28.156781901326934 26.909639045513057 21.417751303874862 94-95 23.782902247098043 28.04530278061353 26.766943440266612 21.404851532021816 96-97 23.88710544552763 28.122111831739627 26.680910042978738 21.309872679754 98-99 24.04033189283484 28.1936074575532 26.56521022687851 21.200850422733446 100-101 24.642057405475075 28.042500418097426 26.112674386963675 21.20276778946382 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 127.0 1 124.0 2 143.5 3 170.5 4 192.0 5 243.5 6 298.0 7 361.5 8 466.0 9 620.5 10 817.0 11 1112.0 12 1471.5 13 1836.5 14 2338.0 15 2827.5 16 3396.0 17 4250.5 18 5243.0 19 6327.0 20 7674.5 21 9277.5 22 11211.0 23 13654.0 24 16316.0 25 19046.5 26 22348.0 27 25883.5 28 29452.5 29 33215.5 30 36887.0 31 40622.0 32 44193.5 33 47358.5 34 50128.5 35 52627.5 36 54261.0 37 54875.0 38 54704.0 39 54099.5 40 52885.5 41 50738.5 42 48367.5 43 45794.5 44 42918.5 45 40242.5 46 38031.0 47 35756.0 48 33451.5 49 31441.0 50 29265.0 51 27419.0 52 25768.0 53 24068.0 54 23048.0 55 22345.5 56 21774.0 57 21318.5 58 20845.0 59 20384.5 60 19836.5 61 19337.5 62 18824.0 63 18154.5 64 17553.0 65 16851.5 66 16160.5 67 15415.0 68 14459.5 69 13496.0 70 12142.5 71 10855.5 72 9750.0 73 8426.5 74 7191.0 75 6012.0 76 4890.5 77 3983.0 78 3289.0 79 2606.0 80 2030.5 81 1645.5 82 1349.0 83 1074.5 84 851.0 85 703.0 86 567.0 87 472.5 88 365.5 89 265.5 90 215.0 91 170.5 92 132.5 93 96.0 94 64.5 95 50.5 96 37.5 97 20.0 98 13.0 99 6.0 100 2.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 14.130614443175382 2 11.535844840020676 3 10.160118610964087 4 12.078322855825153 5 9.499772497237466 6 5.61148718710632 7 10.128175365938967 8 10.559656795832522 9 5.732264844168346 10-11 6.669080981697635 12-13 5.61548009273446 14-15 7.681050412755012 16-17 2.7945386955889226 18-19 3.2796612530295013 20-21 3.2597276776456097 22-23 3.285201796498005 24-25 3.6725445951843705 26-27 4.565160195992856 28-29 1.8180720765895015 30-31 2.3825265497271513 32-33 3.214815227518239 34-35 2.136080700027548 36-37 3.2841803555233646 38-39 1.4544081368607096 40-41 1.232941161904585 42-43 0.5897738282060092 44-45 1.36650230752802 46-47 1.4289959235219285 48-49 1.324808944108607 50-51 1.634088890126999 52-53 2.574031256093824 54-55 1.9036874019184518 56-57 1.9177399535089592 58-59 1.6516700559935371 60-61 1.4929752718425866 62-63 0.6517102945773865 64-65 1.2969824157388576 66-67 0.5975429701646378 68-69 0.8576080328594465 70-71 1.0696034642325418 72-73 0.08694629387166368 74-75 0.10852036536634137 76-77 0.4831106282481049 78-79 0.9335041925509096 80-81 0.31708004073382795 82-83 0.507656164396282 84-85 0.06716748227180855 86-87 0.10075122340771281 88-89 0.08341767959563318 90-91 0.09155825463594915 92-93 0.18320936754232017 94-95 0.4899202347457076 96-97 0.3980524525416856 98-99 0.06865321459855822 100-101 0.24133864482640144 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1615365.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.69031620407713 #Duplication Level Percentage of deduplicated Percentage of total 1 98.72387922976567 97.43090858078696 2 1.2315571504151 2.430855291957168 3 0.03818386994785454 0.11305134597147369 4 0.006379749871382261 0.02518478128438543 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.476220544582803E-4 0.0 0.0 2 2.476220544582803E-4 0.0 0.0 3 3.095275680728504E-4 0.0 0.0 4 4.3333859530199055E-4 0.0 0.0 5 4.3333859530199055E-4 0.0 0.0 6 6.190551361457008E-4 0.0 0.0 7 6.190551361457008E-4 0.0 1.2381102722914015E-4 8 7.42866163374841E-4 0.0 1.2381102722914015E-4 9 8.666771906039811E-4 0.0 1.2381102722914015E-4 10-11 9.904882178331214E-4 0.0 1.2381102722914015E-4 12-13 0.0011762047586768316 0.0 1.2381102722914015E-4 14-15 0.0015166850835569672 0.0 1.2381102722914015E-4 16-17 0.0016404961107861073 0.0 1.2381102722914015E-4 18-19 0.0019190709220516726 3.095275680728504E-5 1.2381102722914015E-4 20-21 0.0021666929765099528 6.190551361457008E-5 1.2381102722914015E-4 22-23 0.0026309843286192285 1.2381102722914015E-4 1.2381102722914015E-4 24-25 0.0027857481126556537 1.2381102722914015E-4 1.2381102722914015E-4 26-27 0.003126228437535789 1.2381102722914015E-4 1.2381102722914015E-4 28-29 0.0039310001145252006 1.2381102722914015E-4 1.547637840364252E-4 30-31 0.005138157630009316 1.2381102722914015E-4 2.476220544582803E-4 32-33 0.007181039579290129 1.2381102722914015E-4 3.4048032488013543E-4 34-35 0.010709653855320625 1.2381102722914015E-4 4.3333859530199055E-4 36-37 0.014733512240267681 1.2381102722914015E-4 4.3333859530199055E-4 38-39 0.021202638412990256 1.547637840364252E-4 4.642913521092756E-4 40-41 0.030581323725597617 1.8571654084371025E-4 4.952441089165606E-4 42-43 0.04472673358652689 1.8571654084371025E-4 4.952441089165606E-4 44-45 0.06184360810095551 1.8571654084371025E-4 5.261968657238457E-4 46-47 0.08289148272990934 1.8571654084371025E-4 5.571496225311308E-4 48-49 0.11161564104706986 1.8571654084371025E-4 5.881023793384158E-4 50-51 0.15163755559888942 1.8571654084371025E-4 6.190551361457008E-4 52-53 0.20066672238162891 1.8571654084371025E-4 6.190551361457008E-4 54-55 0.26059125956053275 1.8571654084371025E-4 6.190551361457008E-4 56-57 0.33178260021728834 1.8571654084371025E-4 6.190551361457008E-4 58-59 0.42590993366824215 1.8571654084371025E-4 6.500078929529858E-4 60-61 0.538825590501218 1.8571654084371025E-4 6.809606497602709E-4 62-63 0.6629770980552383 1.8571654084371025E-4 6.809606497602709E-4 64-65 0.8028835588241667 1.8571654084371025E-4 6.809606497602709E-4 66-67 0.9625069256793356 1.8571654084371025E-4 6.809606497602709E-4 68-69 1.1504520650131704 1.8571654084371025E-4 6.809606497602709E-4 70-71 1.3726619061326697 1.8571654084371025E-4 6.809606497602709E-4 72-73 1.5912502747057167 1.8571654084371025E-4 6.809606497602709E-4 74-75 1.8252221634119843 1.8571654084371025E-4 6.809606497602709E-4 76-77 2.0802419267472056 1.8571654084371025E-4 6.809606497602709E-4 78-79 2.367885895757306 1.8571654084371025E-4 6.809606497602709E-4 80-81 2.6940969997492825 1.8571654084371025E-4 6.809606497602709E-4 82-83 3.040396442909188 1.8571654084371025E-4 6.809606497602709E-4 84-85 3.391586421644644 1.8571654084371025E-4 6.809606497602709E-4 86-87 3.770695787020271 2.476220544582803E-4 7.42866163374841E-4 88-89 4.172555428649252 2.476220544582803E-4 7.42866163374841E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTGGCT 915 0.002395402 6.822417 1 CGTGTAG 3065 0.0 6.2785892 94-95 GTGTAGG 3125 0.0 5.361118 94-95 GTCGTGT 3850 0.0 5.233301 92-93 GTAGGGA 2165 0.0 5.2286043 94-95 >>END_MODULE