##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename NYCLib6_CTTGTA_L007_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1615365 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.257694081523372 33.0 33.0 33.0 27.0 33.0 2 31.65402741795198 33.0 33.0 33.0 27.0 33.0 3 31.828978589978117 33.0 33.0 33.0 27.0 33.0 4 35.65655687723827 37.0 37.0 37.0 33.0 37.0 5 35.174339545551625 37.0 37.0 37.0 33.0 37.0 6 34.97617009158921 37.0 37.0 37.0 33.0 37.0 7 34.76588696672269 37.0 33.0 37.0 27.0 37.0 8 34.80723242115559 37.0 33.0 37.0 27.0 37.0 9 34.700853367505175 37.0 33.0 37.0 27.0 37.0 10-11 34.55231480191783 37.0 33.0 37.0 27.0 37.0 12-13 34.55728705277136 37.0 33.0 37.0 27.0 37.0 14-15 35.48886536479372 37.0 33.0 40.0 27.0 40.0 16-17 35.4054077561418 37.0 33.0 40.0 27.0 40.0 18-19 35.61833424643966 37.0 33.0 40.0 27.0 40.0 20-21 35.29923144304848 37.0 33.0 40.0 27.0 40.0 22-23 35.342544564231616 37.0 33.0 40.0 27.0 40.0 24-25 35.186255118812156 37.0 33.0 40.0 27.0 40.0 26-27 34.817776168234424 37.0 33.0 40.0 27.0 40.0 28-29 34.46142915068731 37.0 33.0 40.0 27.0 40.0 30-31 34.360629331451406 37.0 33.0 40.0 27.0 40.0 32-33 34.37354282159141 37.0 33.0 40.0 27.0 40.0 34-35 34.24505668997409 37.0 33.0 40.0 24.5 40.0 36-37 33.947668174065925 37.0 33.0 37.0 22.0 40.0 38-39 33.862049134406156 37.0 33.0 37.0 22.0 40.0 40-41 33.58076100447886 37.0 33.0 37.0 22.0 40.0 42-43 33.215911883691916 37.0 33.0 37.0 22.0 40.0 44-45 33.12834498704627 37.0 33.0 37.0 22.0 40.0 46-47 32.98106031763719 37.0 33.0 37.0 22.0 40.0 48-49 33.01855834439894 37.0 33.0 37.0 22.0 40.0 50-51 33.324138816923735 37.0 33.0 37.0 22.0 40.0 52-53 33.17812785345727 37.0 33.0 37.0 22.0 40.0 54-55 32.83343702506863 37.0 33.0 37.0 22.0 40.0 56-57 32.890233476644596 37.0 33.0 37.0 22.0 40.0 58-59 32.594986581979924 37.0 33.0 37.0 22.0 40.0 60-61 32.51664608308339 37.0 33.0 37.0 22.0 40.0 62-63 32.509760642331614 37.0 33.0 37.0 22.0 40.0 64-65 32.17648302396053 35.0 27.0 37.0 22.0 40.0 66-67 31.842284251546864 33.0 27.0 37.0 22.0 40.0 68-69 31.6841373311914 33.0 27.0 37.0 22.0 40.0 70-71 31.526155079502153 33.0 27.0 37.0 18.5 40.0 72-73 31.311303637258455 33.0 27.0 37.0 15.0 38.5 74-75 30.967847514338864 33.0 27.0 37.0 15.0 37.0 76-77 29.85730841017355 33.0 27.0 37.0 15.0 37.0 78-79 30.779522275151436 33.0 27.0 37.0 15.0 37.0 80-81 30.483234439275336 33.0 27.0 37.0 15.0 37.0 82-83 30.469262055324958 33.0 27.0 37.0 15.0 37.0 84-85 30.343241620314913 33.0 27.0 37.0 15.0 37.0 86-87 30.14835130140866 33.0 27.0 37.0 15.0 37.0 88-89 30.001609852881547 33.0 27.0 37.0 15.0 37.0 90-91 29.91706549293813 33.0 27.0 37.0 15.0 37.0 92-93 29.713016872347737 33.0 27.0 37.0 15.0 37.0 94-95 29.628858493281705 33.0 27.0 33.0 15.0 37.0 96-97 29.49195166417497 33.0 27.0 33.0 15.0 37.0 98-99 29.223570214781176 33.0 27.0 33.0 15.0 37.0 100-101 28.413065468175922 33.0 24.5 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 1.1314315213035009 2306 2 0.604374306579949 2306 3 0.43483987846012084 2306 4 0.37624604868751277 2306 5 0.7116619591542701 2306 6 0.7660168959604121 2306 7 0.9288305768639802 2306 8 0.5921141686244056 2306 9 1.0492922708702963 2306 10-11 1.1564085354440365 2306 12-13 1.3369195253495292 2306 14-15 1.5436404605416953 2306 16-17 1.4803197789743123 2306 18-19 1.355098155816421 2306 20-21 1.6860469735322852 2306 22-23 1.3145537781390573 2306 24-25 1.1960446484649623 2306 26-27 1.394795343009008 2306 28-29 1.645032018943951 2306 30-31 1.630289986042456 2306 32-33 1.2996525116516935 2306 34-35 1.3689478146343674 2306 36-37 1.4560512688831935 2306 38-39 1.224221650278686 2306 40-41 1.2889628811169445 2306 42-43 1.3574381979409011 2306 44-45 1.228267849482144 2306 46-47 1.1053353792560756 2306 48-49 1.2401142871268576 2306 50-51 1.2010837500546359 2306 52-53 1.4808067584861107 2306 54-55 1.7285773883601365 2306 56-57 1.3271710207034957 2306 58-59 1.29303746185348 2306 60-61 1.139344189490572 2306 62-63 0.7633245481597228 2306 64-65 0.9258436479959222 2306 66-67 1.1340067413784283 2306 68-69 0.9720991772012582 2306 70-71 1.0300448403632245 2306 72-73 0.8511891466202002 2306 74-75 0.9340840956532475 2306 76-77 1.082074179491979 2306 78-79 1.0101573963117403 2306 80-81 1.339670050266129 2306 82-83 1.1401405832818803 2306 84-85 1.0658567633702738 2306 86-87 0.8050687996803347 2306 88-89 0.7473763991358275 2306 90-91 0.4540736398777163 2306 92-93 0.3384627840497423 2306 94-95 0.3527790808817386 2306 96-97 0.277152091687924 2306 98-99 0.3421404697800128 2306 100-101 0.1247973231283801 2307 1 0.7655224639009788 2307 2 0.4723127530293283 2307 3 0.2888963847692594 2307 4 0.3004330192313063 2307 5 0.572841436947293 2307 6 0.6861552618670572 2307 7 0.8606245340204453 2307 8 0.7933641933036597 2307 9 1.046028143193766 2307 10-11 1.1185967286993588 2307 12-13 1.3582441680219617 2307 14-15 1.3967873908606663 2307 16-17 1.4754501419054762 2307 18-19 1.2763819408526658 2307 20-21 1.5220672776824244 2307 22-23 1.2522517456290103 2307 24-25 1.2031706806073927 2307 26-27 1.5393881810162853 2307 28-29 1.6060792762878293 2307 30-31 1.7613788105780088 2307 32-33 1.2867058828981044 2307 34-35 1.301503315366297 2307 36-37 1.3930663215467831 2307 38-39 1.1839942511082029 2307 40-41 1.278755455545479 2307 42-43 1.4328823102727668 2307 44-45 1.1520022736399298 2307 46-47 1.1054478476806366 2307 48-49 1.1353914409661598 2307 50-51 1.088542645625921 2307 52-53 1.336038600784427 2307 54-55 1.6342000422683682 2307 56-57 1.3142659910414096 2307 58-59 1.2691787232293237 2307 60-61 1.0675598488370355 2307 62-63 0.9478190191878397 2307 64-65 0.9842262270378619 2307 66-67 1.1010939712677654 2307 68-69 1.0729143814493511 2307 70-71 0.9093619100180348 2307 72-73 0.8464198215574861 2307 74-75 0.9216729528214529 2307 76-77 1.0382577486526472 2307 78-79 1.0661518426390373 2307 80-81 1.6341657964249343 2307 82-83 1.3516482973053279 2307 84-85 1.062601214159237 2307 86-87 1.0336677914218164 2307 88-89 0.927272122930276 2307 90-91 0.7126378582273354 2307 92-93 0.6927945340886019 2307 94-95 0.5548865210322873 2307 96-97 0.7372094630857333 2307 98-99 0.7188186051509575 2307 100-101 0.6571981442125399 1101 1 1.0279902902568132 1101 2 0.5877311424359206 1101 3 0.36111698890432464 1101 4 0.44462175361465484 1101 5 0.6291510443586787 1101 6 0.6205005474902308 1101 7 0.7628834998936824 1101 8 0.6619568078193439 1101 9 0.8560164877372145 1101 10-11 0.8469209260860069 1101 12-13 1.0931792682018937 1101 14-15 1.2891322030844208 1101 16-17 1.2599963608366735 1101 18-19 1.0655472466702705 1101 20-21 1.3680572480984452 1101 22-23 0.9687515459680895 1101 24-25 1.0085053396682966 1101 26-27 1.005454496677146 1101 28-29 1.2376228338287802 1101 30-31 1.3281527503227721 1101 32-33 0.7786068047197503 1101 34-35 1.0225792811595298 1101 36-37 1.1111670695213718 1101 38-39 0.8218342302479442 1101 40-41 0.9902863048564825 1101 42-43 1.0199650594824377 1101 44-45 0.9919072486391727 1101 46-47 0.9070911764176728 1101 48-49 0.8518290596972946 1101 50-51 0.6881003675009651 1101 52-53 1.020241003792016 1101 54-55 1.3862240601432063 1101 56-57 1.0690221214856024 1101 58-59 1.1876511403046806 1101 60-61 0.7506802395763259 1101 62-63 0.6410264258321945 1101 64-65 0.5642468973024357 1101 66-67 0.7522293106921936 1101 68-69 0.5873324531066118 1101 70-71 0.7398890531831945 1101 72-73 0.6038136374200995 1101 74-75 0.89990485823499 1101 76-77 0.8823267997813566 1101 78-79 0.8900971911497386 1101 80-81 1.3947507880942567 1101 82-83 1.1816899707479145 1101 84-85 1.0409386147884945 1101 86-87 0.9676099206072237 1101 88-89 0.8710690129609482 1101 90-91 0.5915362790149423 1101 92-93 0.7039826481374298 1101 94-95 0.689225331401822 1101 96-97 0.6588869564113438 1101 98-99 0.7325689761925744 1101 100-101 0.9038029499836924 2201 1 1.4833763850383512 2201 2 0.8108262744987087 2201 3 0.22679844855536757 2201 4 0.19852899979287741 2201 5 0.3079327953295419 2201 6 0.5064941162393239 2201 7 0.5652562736881137 2201 8 0.6158428771679283 2201 9 0.5559107073969969 2201 10-11 0.8625004289910692 2201 12-13 1.1179662801616104 2201 14-15 1.1558598742456212 2201 16-17 0.9335295799120118 2201 18-19 0.8866849580017018 2201 20-21 1.2199895992005878 2201 22-23 0.975909971837595 2201 24-25 0.8956715061534695 2201 26-27 0.9866391257590976 2201 28-29 1.2388749311826786 2201 30-31 1.26005903523901 2201 32-33 0.9550279620334905 2201 34-35 0.9354167306526122 2201 36-37 0.9397340590654792 2201 38-39 0.47939563872857605 2201 40-41 0.938451083428852 2201 42-43 0.9890320090604803 2201 44-45 0.9523560590169282 2201 46-47 0.8620692192979789 2201 48-49 1.004271350241325 2201 50-51 0.8404442134692829 2201 52-53 1.0235012666822882 2201 54-55 1.5100693260317968 2201 56-57 1.1558469500034647 2201 58-59 1.2622167644277198 2201 60-61 0.9436942387756133 2201 62-63 0.8318685023933554 2201 64-65 0.775738804950695 2201 66-67 0.677977705734186 2201 68-69 0.7915258806076366 2201 70-71 1.0256230500571917 2201 72-73 0.7930594931456767 2201 74-75 0.9656391907548141 2201 76-77 0.8809523820144101 2201 78-79 0.7859427435483788 2201 80-81 1.1682764464877735 2201 82-83 0.9556785262632168 2201 84-85 0.9925853728131173 2201 86-87 1.0289756669468666 2201 88-89 1.0854626999127355 2201 90-91 0.8750545808823453 2201 92-93 1.0225675480198149 2201 94-95 0.9165495144070022 2201 96-97 0.807190595469585 2201 98-99 0.9056650771299282 2201 100-101 1.006297767369869 2304 1 -1.734191857035178 2304 2 -0.3190521305143754 2304 3 -0.12690091524700975 2304 4 -0.00451030548421727 2304 5 0.2959140340638342 2304 6 0.5049631938634249 2304 7 0.41814451721413803 2304 8 0.6860139128900897 2304 9 0.9210377352782615 2304 10-11 0.9781012370602511 2304 12-13 1.1765494152628762 2304 14-15 1.1813011720208735 2304 16-17 1.1572708770211833 2304 18-19 0.8565501208048261 2304 20-21 1.122293277475265 2304 22-23 0.9707205209993219 2304 24-25 1.0891019905673929 2304 26-27 1.0267040089352122 2304 28-29 1.4152112437804902 2304 30-31 1.425487660199991 2304 32-33 0.8389372588040231 2304 34-35 0.861013473451429 2304 36-37 1.198939307303739 2304 38-39 0.9817358550172912 2304 40-41 0.9565162360473067 2304 42-43 0.8386050804689305 2304 44-45 0.8650946653554357 2304 46-47 0.9428575508547326 2304 48-49 0.6917355347152068 2304 50-51 0.7638501794898929 2304 52-53 0.8582483486368062 2304 54-55 1.3845695201634953 2304 56-57 0.9925345432151431 2304 58-59 0.6973386913830595 2304 60-61 0.3334206108419622 2304 62-63 0.21729695760995327 2304 64-65 0.429934595848831 2304 66-67 0.5250090699826302 2304 68-69 0.4518195233369795 2304 70-71 0.3596193891652 2304 72-73 0.26161778540553016 2304 74-75 0.5398432668974138 2304 76-77 0.5781153626139179 2304 78-79 0.6079162926475838 2304 80-81 1.1377935796041037 2304 82-83 0.6908659122363581 2304 84-85 0.3959961496901485 2304 86-87 0.34970519389507615 2304 88-89 0.21878793636772187 2304 90-91 0.09083894923021418 2304 92-93 0.14675317789069808 2304 94-95 0.03852234370410912 2304 96-97 -0.007507043601016505 2304 98-99 0.3074437296045325 2304 100-101 0.4109426288315987 1102 1 1.4709535010255728 1102 2 0.1672203459533037 1102 3 0.31704812299517826 1102 4 0.12487596417032165 1102 5 0.32139858859901693 1102 6 0.23815608657348974 1102 7 -4.625356426355239 1102 8 -1.1461202565578006 1102 9 0.20823841462924264 1102 10-11 0.7222033355832167 1102 12-13 1.0156784834128203 1102 14-15 0.9257731454728741 1102 16-17 0.6493133193837721 1102 18-19 0.8563115013803397 1102 20-21 1.1302505979753619 1102 22-23 0.795773533046372 1102 24-25 1.2018123491522132 1102 26-27 1.1353755633181493 1102 28-29 0.979321732221706 1102 30-31 1.276937434753549 1102 32-33 0.9683682288426709 1102 34-35 1.0684233743138023 1102 36-37 1.1075191369727406 1102 38-39 0.7877149048839485 1102 40-41 1.2633485059778735 1102 42-43 1.1580517513692925 1102 44-45 1.0535291345923312 1102 46-47 0.9861708002382912 1102 48-49 0.920599782138595 1102 50-51 0.7065668743838103 1102 52-53 0.8443653815936685 1102 54-55 1.411329577038515 1102 56-57 1.308127969863591 1102 58-59 1.1391063034529694 1102 60-61 1.0193372153391351 1102 62-63 0.7684614678289066 1102 64-65 0.471161748261256 1102 66-67 0.4896115827844838 1102 68-69 0.7682790347487369 1102 70-71 0.7159597651606404 1102 72-73 0.561826276533985 1102 74-75 0.7578096318147516 1102 76-77 0.7277797777408104 1102 78-79 0.8138752688404196 1102 80-81 1.465709372617379 1102 82-83 1.1507593522119244 1102 84-85 0.9246165488112226 1102 86-87 0.6970452009048564 1102 88-89 0.6102570636976559 1102 90-91 0.2983900653644227 1102 92-93 0.5899373297192874 1102 94-95 0.4070034388801176 1102 96-97 0.3075531205074924 1102 98-99 0.3146463605956278 1102 100-101 0.3483971281090419 2202 1 1.6357528924925582 2202 2 1.0158713224134033 2202 3 0.39572587816865124 2202 4 -0.32001712731903353 2202 5 0.275686137771018 2202 6 0.46834793954734977 2202 7 0.7374166785642302 2202 8 -3.6287707734593155 2202 9 -0.25944766201921965 2202 10-11 0.6417923643606542 2202 12-13 0.1651750706043842 2202 14-15 0.17695961672015414 2202 16-17 0.33664286365144136 2202 18-19 0.9470691642141205 2202 20-21 1.20845548417347 2202 22-23 -2.5870060372816255 2202 24-25 -2.734974717279478 2202 26-27 -1.3675855199154512 2202 28-29 0.6360884649690206 2202 30-31 1.284980920102953 2202 32-33 1.33991064910645 2202 34-35 -1.8535888452573843 2202 36-37 -0.15600911900240533 2202 38-39 0.5434121958828655 2202 40-41 1.3342540784059835 2202 42-43 1.580478248743134 2202 44-45 1.4447364719394074 2202 46-47 0.8470451690457779 2202 48-49 1.1482409448320183 2202 50-51 -3.621779267489366 2202 52-53 -0.06628053258911137 2202 54-55 1.6681179375708837 2202 56-57 1.5480106117446653 2202 58-59 1.5665480141521755 2202 60-61 1.5395251860109411 2202 62-63 1.3600578123511227 2202 64-65 1.2909805181217457 2202 66-67 1.494000316364314 2202 68-69 1.3717222596476404 2202 70-71 1.3828437923408359 2202 72-73 1.3283004584611042 2202 74-75 0.8650260645497951 2202 76-77 1.3479107239169217 2202 78-79 1.4249636228003943 2202 80-81 2.055143893924946 2202 82-83 1.8254415460488467 2202 84-85 1.7376939584496256 2202 86-87 1.702379035574829 2202 88-89 1.5572697545456649 2202 90-91 1.381535015340301 2202 92-93 1.5027927679736202 2202 94-95 1.3904176152945737 2202 96-97 1.5170484059560465 2202 98-99 1.5456642854185674 2202 100-101 1.415171386000889 2305 1 1.2722217595245873 2305 2 0.5811037741906624 2305 3 0.03216036721164528 2305 4 -0.15717120424391595 2305 5 0.17554523153241774 2305 6 0.3394718212835883 2305 7 0.5708248601774883 2305 8 -0.05018225535952325 2305 9 0.511575348604886 2305 10-11 0.2988455962832717 2305 12-13 0.8206788996160981 2305 14-15 0.5182222984992109 2305 16-17 0.5491323435516264 2305 18-19 0.6351470081659087 2305 20-21 0.6234252271914755 2305 22-23 0.4206243532088081 2305 24-25 0.6627653433476368 2305 26-27 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-0.28546066748974397 1208 40-41 -0.32537547683922696 1208 42-43 0.38838184343042315 1208 44-45 0.26106903150186866 1208 46-47 -0.1779926242904537 1208 48-49 -1.5180820398996318 1208 50-51 -0.0759010375408451 1208 52-53 -1.385306110620867 1208 54-55 -0.7504944436973204 1208 56-57 -6.264293123684855 1208 58-59 -2.1815311813752096 1208 60-61 -1.2568461866461433 1208 62-63 0.42766429147565077 1208 64-65 0.01631793360246192 1208 66-67 0.7191804866561782 1208 68-69 0.48239951934663594 1208 70-71 -4.418120385336891 1208 72-73 -0.7774095658418396 1208 74-75 -5.478015213090721 1208 76-77 -1.174764110158268 1208 78-79 -3.660428299156173 1208 80-81 0.9563433166327115 1208 82-83 0.5039195060338351 1208 84-85 -2.581845001770457 1208 86-87 -0.7360832154601056 1208 88-89 -4.770795864961457 1208 90-91 0.28463500304092193 1208 92-93 -2.0308892293205183 1208 94-95 -4.552447111807179 1208 96-97 -3.327029366580227 1208 98-99 -6.585983410628039 1208 100-101 -3.69659218885171 1209 1 1.8504229748453263 1209 2 1.280584100842482 1209 3 0.7060062249476324 1209 4 0.22349089809598865 1209 5 0.5217631643873233 1209 6 1.0274175992813355 1209 7 0.7685399472021501 1209 8 0.33703762970743156 1209 9 0.0526707870277221 1209 10-11 -2.799431782803314 1209 12-13 -0.48721518600626723 1209 14-15 -1.2857934841728422 1209 16-17 0.011556386005374009 1209 18-19 -16.772572478209856 1209 20-21 -14.083432261328966 1209 22-23 -3.133502788037859 1209 24-25 -1.939826149407189 1209 26-27 -1.4061784309194607 1209 28-29 -3.3515340148769823 1209 30-31 -1.0398406971375778 1209 32-33 -0.18538134863572253 1209 34-35 -3.025955076279338 1209 36-37 -3.9023046916363953 1209 38-39 -1.2352368713164168 1209 40-41 -10.285402037264198 1209 42-43 -4.94763458468428 1209 44-45 -1.9865995534177188 1209 46-47 -2.1636795063916736 1209 48-49 -3.2756124122374395 1209 50-51 -2.1097114748041434 1209 52-53 -0.8246352139605975 1209 54-55 -2.438311078393106 1209 56-57 -0.6513916431870896 1209 58-59 -1.6505391003167276 1209 60-61 -1.2774403531898457 1209 62-63 -0.5555313211356321 1209 64-65 -0.022939850077634105 1209 66-67 0.506325732831975 1209 68-69 -0.7255046663795923 1209 70-71 -0.6057107645607367 1209 72-73 0.4509018797338804 1209 74-75 1.285842934565565 1209 76-77 -16.253847775496915 1209 78-79 -16.816726562891354 1209 80-81 -22.008109981286722 1209 82-83 -9.706645640786292 1209 84-85 -3.5033073007227955 1209 86-87 -2.222574288206161 1209 88-89 -1.8170257601953388 1209 90-91 -6.195073324144165 1209 92-93 -3.4025015454879473 1209 94-95 -0.8861690143205827 1209 96-97 0.10582440352454014 1209 98-99 1.1438232885013697 1209 100-101 1.0061646848878958 1212 1 -28.648342457253438 1212 2 -16.279942214946992 1212 3 -5.897108622355816 1212 4 -1.2788157945615808 1212 5 -0.6382270890239852 1212 6 -3.096623986163763 1212 7 -0.8131973822325449 1212 8 -1.4368333904355168 1212 9 -0.11718788743621644 1212 10-11 0.5443283698932362 1212 12-13 0.3494807204264774 1212 14-15 -0.15693545948148824 1212 16-17 -0.07733656396214172 1212 18-19 -0.9040458375340918 1212 20-21 -1.0207413906337095 1212 22-23 -0.6758070440482555 1212 24-25 0.15357459783128036 1212 26-27 -0.8092201788076991 1212 28-29 0.015837248930687053 1212 30-31 -0.8321084034403725 1212 32-33 -0.19884382426924674 1212 34-35 -1.2787308235178045 1212 36-37 -1.4374066247098156 1212 38-39 -0.4168747043248544 1212 40-41 -0.3634173881413929 1212 42-43 -0.18565643328727077 1212 44-45 0.8983663335218566 1212 46-47 -2.73199700151838 1212 48-49 -1.4869346994369188 1212 50-51 0.026419941700083882 1212 52-53 -0.43405082474681933 1212 54-55 -0.3629983753391741 1212 56-57 -1.5036536639798115 1212 58-59 -0.5264792400178315 1212 60-61 0.07824109580300842 1212 62-63 -4.678657133351358 1212 64-65 -3.948079957290112 1212 66-67 -1.703075664179078 1212 68-69 -0.12745601465769596 1212 70-71 -0.9141963883424111 1212 72-73 -1.4915912164324645 1212 74-75 -0.2495595183259205 1212 76-77 0.7414373122223843 1212 78-79 -1.6391843276736822 1212 80-81 0.3447043461986574 1212 82-83 0.6273577705197511 1212 84-85 -3.047745653549306 1212 86-87 -1.6589717053601554 1212 88-89 -0.9041724463551795 1212 90-91 -0.29214122537873166 1212 92-93 -2.2968931959102967 1212 94-95 -1.820030287874836 1212 96-97 -0.09809859842477664 1212 98-99 -0.5541360194908336 1212 100-101 0.2855668941146696 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 3.0 10 4.0 11 8.0 12 31.0 13 127.0 14 432.0 15 1006.0 16 1939.0 17 3203.0 18 5042.0 19 7234.0 20 10211.0 21 13790.0 22 18002.0 23 23542.0 24 29475.0 25 36517.0 26 45585.0 27 54686.0 28 65989.0 29 77795.0 30 91366.0 31 107276.0 32 124463.0 33 141484.0 34 158338.0 35 174635.0 36 184876.0 37 172705.0 38 65436.0 39 165.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.77101308797818 26.18194429110787 17.92809128089784 22.118951340016103 2 22.75716524085127 29.715049466580574 27.111623423777015 20.416161868791143 3 18.983817848652933 26.546156893819333 32.402003268621236 22.068021988906498 4 22.01589114534486 26.817035159236458 29.654288659219436 21.51278503619925 5 20.52136823566191 28.249714460818453 29.006323648215727 22.22259365530391 6 20.451910249386362 28.801106870583425 28.62071420391057 22.12626867611964 7 21.552652186967034 28.649933606336646 29.19575451987631 20.601659686820007 8 20.641898270669476 27.932572514571007 29.458481519656548 21.967047695102966 9 21.2444865401937 27.73509392614053 29.13384900626174 21.88657052740402 10-11 21.53909487948544 27.97835164188898 28.376868385782778 22.105685092842794 12-13 21.51183040414952 28.04374438052314 28.293567829314913 22.15085738601243 14-15 21.38655969393914 28.38160415757429 28.25472880742123 21.977107341065334 16-17 21.643510314577032 28.227803160844385 28.174966625076337 21.953719899502246 18-19 21.91894345268995 28.224440063582755 27.89688783544351 21.959728648283782 20-21 21.713155672653347 28.16464276782199 28.09468949413259 22.02751206539207 22-23 21.568623809479593 28.02375933612527 28.301374611929813 22.106242242465328 24-25 21.685408560913476 27.92879627824052 28.384978008066287 22.00081715277971 26-27 21.756166562974933 28.11974383498466 28.126398677698226 21.997690924342177 28-29 21.954388017568785 28.08216718822062 28.055021620500632 21.908423173709966 30-31 21.94157357625057 27.94671792443194 28.011811571997658 22.099896927319833 32-33 21.73790443645863 28.03211658046324 28.09163873180365 22.138340251274478 34-35 21.887003865999326 27.9928684848316 28.111850881998805 22.00827676717027 36-37 21.96426194699031 27.990856555639127 28.12757488245691 21.917306614913656 38-39 21.821012588486195 27.99450898094239 28.23287616111529 21.95160226945613 40-41 21.9741031404709 27.954858787455944 28.015279840478513 22.055758231594638 42-43 21.747685507609734 27.954022775038457 28.280605312112122 22.017686405239683 44-45 21.767990516075315 27.888991032986354 28.312579509894046 22.030438941044284 46-47 21.890798645780603 28.045840357533734 28.14057998609866 21.922781010587 48-49 22.035951682671843 27.906274907335042 28.143621013859114 21.914152396133996 50-51 21.893177017687655 27.99588823324031 28.035755408688072 22.07517934038396 52-53 21.85339204048627 28.00458101509723 28.198345572588195 21.943681371828305 54-55 21.740033466825064 27.871934606591463 28.380251477870477 22.00778044871299 56-57 21.910651257363366 28.019100569568906 28.12886043812945 21.941387734938274 58-59 21.93733456074299 27.930995698385665 28.174752811324804 21.95691692954654 60-61 22.040942540163446 27.858149723556135 28.049533936262215 22.0513738000182 62-63 21.87113472903019 27.967166167496003 28.160963657321712 22.000735446152095 64-65 21.9340214699363 28.01482794366595 28.091159166695817 21.95999141970194 66-67 22.039049286444804 28.01731823100715 28.026294936147767 21.91733754640028 68-69 21.986962668186315 28.095453153918086 27.967611897470697 21.949972280424905 70-71 22.147850440906623 28.050161331461243 27.779191336884356 22.02279689074777 72-73 21.95669662680583 28.067828037512825 27.9791119222692 21.996363413412144 74-75 21.91093769354639 27.99950452124365 28.067199306329744 22.022358478880218 76-77 21.965461836929716 28.202404450472002 27.918606864844385 21.913526847753896 78-79 22.17236741711974 28.167913294735065 27.679383665844863 21.980335622300327 80-81 21.99822104865153 28.059269452244145 27.76354203469885 22.178967464405478 82-83 22.04152877858737 28.170399176122917 27.680610813108114 22.1074612321816 84-85 21.950965081851262 28.061741713111214 27.827478560185483 22.159814644852048 86-87 21.976543627194236 28.217760178894462 27.728487284352806 22.077208909558497 88-89 22.11848131735059 28.22205589007715 27.551599079271092 22.107863713301164 90-91 22.205125426667387 28.127462286859807 27.381326480855115 22.28608580561769 92-93 21.98612670096193 28.150947040872616 27.519039567505722 22.34388669065973 94-95 22.089292198704072 28.135224567845857 27.422900431573698 22.352582801876373 96-97 22.11519739178053 28.205644930369367 27.38258972642113 22.29656795142897 98-99 22.046792912618745 28.175303007930076 27.399507361561138 22.378396717890045 100-101 22.188080532973576 27.993433486186703 27.226328959062684 22.59215702177704 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2.0 1 3.0 2 7.5 3 18.0 4 33.0 5 46.5 6 67.5 7 114.0 8 176.0 9 236.5 10 311.5 11 413.0 12 538.0 13 733.0 14 1007.5 15 1302.5 16 1703.0 17 2223.5 18 2812.5 19 3651.5 20 4817.0 21 6277.5 22 8018.0 23 10210.5 24 12827.5 25 15722.0 26 19107.0 27 22927.5 28 26856.0 29 30864.0 30 35133.0 31 39218.5 32 43139.0 33 46859.0 34 50166.0 35 52820.5 36 55095.0 37 56423.5 38 56793.5 39 56447.5 40 55288.0 41 53459.0 42 51005.0 43 48134.0 44 45063.5 45 42511.5 46 40134.5 47 37607.0 48 35230.5 49 33035.0 50 30686.0 51 28636.5 52 26850.0 53 25210.5 54 23984.0 55 23309.5 56 22834.5 57 22307.5 58 21915.0 59 21523.5 60 21198.0 61 20904.5 62 20517.5 63 20044.5 64 19434.5 65 18878.0 66 18253.5 67 17416.0 68 16403.0 69 15261.0 70 13902.5 71 12444.0 72 10927.0 73 9103.0 74 7293.0 75 5747.0 76 4349.5 77 3135.5 78 2155.0 79 1443.0 80 936.0 81 564.0 82 373.0 83 266.5 84 178.5 85 116.5 86 86.0 87 63.5 88 44.0 89 32.5 90 20.0 91 11.0 92 7.5 93 4.0 94 2.5 95 1.0 96 0.0 97 1.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7514091242536517 2 0.0019809764356662424 3 3.095275680728504E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.19973813967741036 14-15 0.0 16-17 3.4048032488013543E-4 18-19 0.1259467674488428 20-21 6.190551361457008E-5 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0021047874628953825 42-43 0.0 44-45 0.0 46-47 0.025536024366010157 48-49 1.547637840364252E-4 50-51 6.190551361457008E-5 52-53 1.547637840364252E-4 54-55 0.0021047874628953825 56-57 0.0012690630290986867 58-59 0.10328934946591019 60-61 0.0017333543812079622 62-63 0.0011762047586768316 64-65 0.002259551246931808 66-67 0.004519102493863616 68-69 0.004983393845972892 70-71 0.0035286142760304947 72-73 0.04110526104007453 74-75 0.04735771791514611 76-77 0.17160208373958827 78-79 4.6429135210927563E-4 80-81 0.12721583047794152 82-83 0.004673866277900041 84-85 0.005416732441274882 86-87 0.0068715120112172795 88-89 0.00761437817459212 90-91 0.023369331389500203 92-93 0.010895370396164334 94-95 0.014207315374543833 96-97 0.010121551475982208 98-99 0.01142156726188818 100-101 0.012412055479721303 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1615365.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.90145701849373 #Duplication Level Percentage of deduplicated Percentage of total 1 98.05109386431063 95.99344951573102 2 1.8423038068049604 3.6072845391384645 3 0.08550347265337066 0.2511274365871777 4 0.012857317709369796 0.05035000548387941 5 0.007733500121632575 0.03785604648802639 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 4.4308420884534423E-4 0.013447362784632188 >50 0.0 0.0 >100 0.0 0.0 >500 6.49541911920171E-5 0.046485093786812454 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.476220544582803E-4 0.0 0.0 2 2.476220544582803E-4 0.0 0.0 3 3.095275680728504E-4 0.0 0.0 4 4.952441089165606E-4 6.190551361457008E-5 0.0 5 5.571496225311308E-4 6.190551361457008E-5 0.0 6 8.666771906039811E-4 1.2381102722914015E-4 0.0 7 0.0010523937314476915 1.2381102722914015E-4 0.0 8 0.0012381102722914016 1.2381102722914015E-4 0.0 9 0.0013000157859059717 1.2381102722914015E-4 0.0 10-11 0.0014547795699423969 1.2381102722914015E-4 0.0 12-13 0.0016714488675933922 1.2381102722914015E-4 0.0 14-15 0.0019190709220516726 1.2381102722914015E-4 0.0 16-17 0.0020428819492808127 1.2381102722914015E-4 0.0 18-19 0.002352409517353663 1.2381102722914015E-4 0.0 20-21 0.002754795355848369 1.2381102722914015E-4 0.0 22-23 0.0032809922215722145 1.2381102722914015E-4 0.0 24-25 0.0034667087624159244 1.2381102722914015E-4 0.0 26-27 0.0038690946009106303 1.2381102722914015E-4 0.0 28-29 0.004828630061936467 1.2381102722914015E-4 0.0 30-31 0.006561984443144429 1.2381102722914015E-4 0.0 32-33 0.009100110501341803 1.2381102722914015E-4 0.0 34-35 0.012845394075023293 1.2381102722914015E-4 0.0 36-37 0.01720973278485048 1.2381102722914015E-4 6.190551361457008E-5 38-39 0.02429791409371876 1.2381102722914015E-4 6.190551361457008E-5 40-41 0.03565757584199237 1.2381102722914015E-4 6.190551361457008E-5 42-43 0.05166015111135873 1.2381102722914015E-4 6.190551361457008E-5 44-45 0.0707580020614536 1.2381102722914015E-4 6.190551361457008E-5 46-47 0.09462257755987037 1.2381102722914015E-4 1.2381102722914015E-4 48-49 0.12638010604414482 1.2381102722914015E-4 1.2381102722914015E-4 50-51 0.17178780028043197 1.2381102722914015E-4 1.2381102722914015E-4 52-53 0.22586226642275892 1.2381102722914015E-4 1.2381102722914015E-4 54-55 0.2885106462007039 1.2381102722914015E-4 1.8571654084371025E-4 56-57 0.36632587681421847 1.2381102722914015E-4 2.476220544582803E-4 58-59 0.4654985096247597 1.547637840364252E-4 2.476220544582803E-4 60-61 0.5852547257121455 1.8571654084371025E-4 2.785748112655653E-4 62-63 0.7154420208435864 1.8571654084371025E-4 3.095275680728504E-4 64-65 0.8608890250810188 1.8571654084371025E-4 3.095275680728504E-4 66-67 1.0262386519455355 1.8571654084371025E-4 3.095275680728504E-4 68-69 1.2187957520436559 1.8571654084371025E-4 3.095275680728504E-4 70-71 1.4482794910128671 1.8571654084371025E-4 3.095275680728504E-4 72-73 1.6874204901059513 1.8571654084371025E-4 3.095275680728504E-4 74-75 1.936930662729476 1.8571654084371025E-4 3.714330816874205E-4 76-77 2.2162483401584163 1.8571654084371025E-4 4.3333859530199055E-4 78-79 2.5319045540791087 2.785748112655653E-4 4.3333859530199055E-4 80-81 2.884394548600471 3.095275680728504E-4 4.952441089165606E-4 82-83 3.2571276460737977 3.095275680728504E-4 4.952441089165606E-4 84-85 3.6362989169630393 3.095275680728504E-4 4.952441089165606E-4 86-87 4.0497348896379455 3.714330816874205E-4 5.261968657238457E-4 88-89 4.486973532297654 3.714330816874205E-4 5.881023793384158E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTCTT 725 0.0 9.489544 50-51 CGTATGC 725 7.4578566E-11 7.8559694 44-45 TATGCCG 705 3.4197E-10 7.7422156 46-47 GCCGTCT 770 3.45608E-11 7.7021947 48-49 CGTCTTC 955 0.0 7.452522 50-51 CTCGTAT 790 4.9294613E-10 7.2095923 42-43 ATGCCGT 790 4.9294613E-10 7.2095923 46-47 TCTCGTA 1035 1.8189894E-12 6.8780785 94-95 TCGTATG 755 7.992821E-8 6.6008353 42-43 CCAGTCA 3120 0.0 6.464732 94-95 GTATGCC 905 9.282303E-9 6.293456 44-45 TGCCGTC 1040 7.366907E-10 6.1587934 48-49 ACGTCTG 5385 0.0 6.081064 94-95 GTTGATT 1505 3.099778E-6 6.0344443 1 CGTCTGA 4975 0.0 5.914456 94-95 GTAATCT 1645 0.0 5.9143114 94-95 CTCCAGT 3425 0.0 5.8200283 92-93 CCGGAGC 505 0.0067218514 5.641982 68-69 AATCTCG 1100 1.6789272E-8 5.6090207 92-93 TCCAGTC 3360 0.0 5.5086036 94-95 >>END_MODULE