FastQCFastQC Report
Thu 11 Sep 2014
NYCLib6_CTTGTA_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNYCLib6_CTTGTA_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1615365
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT7250.09.48954450-51
CGTATGC7257.4578566E-117.855969444-45
TATGCCG7053.4197E-107.742215646-47
GCCGTCT7703.45608E-117.702194748-49
CGTCTTC9550.07.45252250-51
CTCGTAT7904.9294613E-107.209592342-43
ATGCCGT7904.9294613E-107.209592346-47
TCTCGTA10351.8189894E-126.878078594-95
TCGTATG7557.992821E-86.600835342-43
CCAGTCA31200.06.46473294-95
GTATGCC9059.282303E-96.29345644-45
TGCCGTC10407.366907E-106.158793448-49
ACGTCTG53850.06.08106494-95
GTTGATT15053.099778E-66.03444431
CGTCTGA49750.05.91445694-95
GTAATCT16450.05.914311494-95
CTCCAGT34250.05.820028392-93
CCGGAGC5050.00672185145.64198268-69
AATCTCG11001.6789272E-85.609020792-93
TCCAGTC33600.05.508603694-95
Produced by FastQC (version 0.11.2)