##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename NYCLib5_CAGATC_L007_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2011052 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.94192193936308 33.0 22.0 33.0 2.0 33.0 2 24.971739666602357 33.0 15.0 33.0 2.0 33.0 3 25.848932300109595 33.0 22.0 33.0 2.0 33.0 4 27.995387488737236 33.0 22.0 37.0 2.0 37.0 5 28.05225324854852 33.0 22.0 37.0 6.0 37.0 6 29.129907133182037 33.0 27.0 37.0 15.0 37.0 7 28.795670126878868 33.0 27.0 37.0 6.0 37.0 8 28.567449275304668 33.0 27.0 37.0 2.0 37.0 9 29.340434260277707 33.0 27.0 37.0 15.0 37.0 10-11 28.948000847317722 33.0 27.0 37.0 10.5 37.0 12-13 29.24824420253678 33.0 27.0 37.0 10.5 37.0 14-15 29.593314344929915 33.0 27.0 37.0 6.0 40.0 16-17 30.503456151307873 33.0 27.0 37.0 15.0 40.0 18-19 30.820624479128334 33.0 27.0 37.0 15.0 40.0 20-21 30.839447214691617 33.0 27.0 37.0 15.0 40.0 22-23 30.86604498541062 33.0 27.0 37.0 15.0 40.0 24-25 30.43373493077255 33.0 27.0 37.0 10.5 40.0 26-27 29.91273447926757 33.0 27.0 37.0 10.5 40.0 28-29 30.121096321726142 33.0 27.0 37.0 15.0 40.0 30-31 30.02448047091771 33.0 27.0 37.0 15.0 40.0 32-33 29.855341880766883 33.0 27.0 37.0 15.0 40.0 34-35 29.899403148203028 33.0 27.0 37.0 15.0 40.0 36-37 30.1397567044512 33.0 27.0 37.0 15.0 40.0 38-39 30.189858840049887 33.0 27.0 37.0 15.0 40.0 40-41 30.26827724991696 33.0 27.0 37.0 15.0 40.0 42-43 30.16421355589015 33.0 27.0 37.0 15.0 40.0 44-45 29.90722641682065 33.0 27.0 37.0 15.0 40.0 46-47 29.50374207131392 33.0 27.0 37.0 15.0 40.0 48-49 29.33995764405893 33.0 24.5 37.0 10.5 40.0 50-51 28.531959889649794 33.0 24.5 37.0 10.5 38.5 52-53 28.21318145925615 33.0 22.0 37.0 10.5 37.0 54-55 29.152038087528318 33.0 24.5 37.0 15.0 40.0 56-57 29.04385863717099 33.0 22.0 37.0 10.5 40.0 58-59 29.214471580048652 33.0 27.0 37.0 15.0 40.0 60-61 28.96118499173567 33.0 24.5 37.0 10.5 40.0 62-63 28.823115463946234 33.0 22.0 37.0 15.0 40.0 64-65 28.765625652643493 33.0 22.0 37.0 10.5 40.0 66-67 28.614640993867887 33.0 22.0 37.0 15.0 40.0 68-69 28.31601942664834 33.0 22.0 37.0 15.0 38.5 70-71 28.186609794276826 33.0 22.0 37.0 10.5 37.0 72-73 27.920936902675813 33.0 22.0 37.0 15.0 37.0 74-75 27.856311771152612 33.0 22.0 37.0 15.0 37.0 76-77 27.425989482121793 30.0 22.0 35.0 6.0 37.0 78-79 26.822451135027837 27.0 22.0 33.0 6.0 37.0 80-81 26.686864138769163 27.0 22.0 33.0 6.0 37.0 82-83 26.218428464306243 27.0 22.0 33.0 6.0 37.0 84-85 26.283019782680906 27.0 22.0 33.0 6.0 37.0 86-87 25.95144705358191 27.0 22.0 33.0 6.0 37.0 88-89 25.544345198433454 27.0 22.0 33.0 6.0 37.0 90-91 25.360118485250506 27.0 22.0 33.0 6.0 37.0 92-93 25.20678281814692 27.0 18.5 33.0 6.0 37.0 94-95 24.939725079212273 27.0 15.0 33.0 6.0 37.0 96-97 24.75847566348359 27.0 15.0 33.0 6.0 37.0 98-99 24.472433333399636 27.0 15.0 33.0 6.0 37.0 100-101 23.939282773394222 27.0 15.0 33.0 6.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 8.283523594816142 2306 2 8.557839301174734 2306 3 7.537268238665163 2306 4 9.057502242605366 2306 5 8.975935159225845 2306 6 7.973776139288887 2306 7 8.226639907106872 2306 8 8.680808799533711 2306 9 7.6574172740825155 2306 10-11 7.823103566952604 2306 12-13 7.469675498579914 2306 14-15 8.168146604079048 2306 16-17 6.934981657054379 2306 18-19 6.292254420690121 2306 20-21 6.169970565384961 2306 22-23 5.820087749435459 2306 24-25 5.977693306113295 2306 26-27 6.508177549383117 2306 28-29 5.637719351079536 2306 30-31 5.511028162786538 2306 32-33 5.580175715844415 2306 34-35 4.930605429511203 2306 36-37 4.960235594535096 2306 38-39 4.608951428149567 2306 40-41 3.9193294906279235 2306 42-43 3.615043349768982 2306 44-45 3.20965882572737 2306 46-47 3.5873670092381964 2306 48-49 3.3422533402533006 2306 50-51 3.210031090608471 2306 52-53 3.695959695598571 2306 54-55 3.180505308827975 2306 56-57 3.4911982876247123 2306 58-59 3.0362832334022407 2306 60-61 3.1098799013675844 2306 62-63 2.602384332307988 2306 64-65 2.159395030705852 2306 66-67 2.022888771974767 2306 68-69 2.3283601216721834 2306 70-71 2.3326864636751523 2306 72-73 1.4190934835360807 2306 74-75 1.1162962544350883 2306 76-77 1.577952222746415 2306 78-79 1.9119011508832173 2306 80-81 1.489302764726812 2306 82-83 1.502237129930272 2306 84-85 1.0141231805152628 2306 86-87 1.092654531335306 2306 88-89 0.9960154677574238 2306 90-91 0.7997104593206288 2306 92-93 0.7133196181169694 2306 94-95 0.8628780070745918 2306 96-97 0.696839327044227 2306 98-99 0.534007969207476 2306 100-101 0.4268284799121389 2307 1 8.3324956144493 2307 2 8.572941922599348 2307 3 7.644493623793945 2307 4 9.16186166081468 2307 5 9.105379323973946 2307 6 7.715574852550034 2307 7 8.215951224691786 2307 8 8.537717155094366 2307 9 7.593785307908309 2307 10-11 7.924540791282425 2307 12-13 7.510204779879988 2307 14-15 8.35861732539545 2307 16-17 7.073133014906844 2307 18-19 6.508316776088989 2307 20-21 6.342059377053918 2307 22-23 6.124184844210511 2307 24-25 6.246645825375467 2307 26-27 6.99957575116699 2307 28-29 6.124341940679091 2307 30-31 5.87001669782866 2307 32-33 6.07448264756292 2307 34-35 5.7649150987167985 2307 36-37 5.698718852255926 2307 38-39 5.201565462311578 2307 40-41 4.625637708561989 2307 42-43 4.440460410451358 2307 44-45 4.380632882722484 2307 46-47 4.695455122526333 2307 48-49 4.447627678257838 2307 50-51 4.238039148479668 2307 52-53 4.779180747626526 2307 54-55 4.384804864267046 2307 56-57 4.878161629500106 2307 58-59 4.294437696138509 2307 60-61 4.20461377096203 2307 62-63 3.8946147312572137 2307 64-65 3.805713579355828 2307 66-67 3.3630074520154025 2307 68-69 3.4785439944609102 2307 70-71 3.256209648806248 2307 72-73 2.6294892079540944 2307 74-75 2.316005095264657 2307 76-77 2.441153528018635 2307 78-79 2.889372564257286 2307 80-81 2.871037598928414 2307 82-83 2.842177589858629 2307 84-85 2.17018362915789 2307 86-87 2.201213146810055 2307 88-89 2.2571807302127738 2307 90-91 1.9920002577059535 2307 92-93 1.870954121835961 2307 94-95 1.8283960811395907 2307 96-97 1.7544495575055343 2307 98-99 1.5090406369894893 2307 100-101 1.1242093665178103 1101 1 8.165859585078149 1101 2 8.060567401104564 1101 3 7.422656663107929 1101 4 8.908742511635943 1101 5 8.766254842138256 1101 6 7.610572371370978 1101 7 7.303759444354128 1101 8 8.270020471390122 1101 9 7.64639260067462 1101 10-11 7.660465844650972 1101 12-13 7.3331063166202135 1101 14-15 7.797815071609932 1101 16-17 7.061463188032604 1101 18-19 6.167958001574917 1101 20-21 5.956883339278125 1101 22-23 5.499299457891148 1101 24-25 6.06327780046027 1101 26-27 6.643965955569303 1101 28-29 6.01136936223693 1101 30-31 5.884668082229361 1101 32-33 5.990527436048485 1101 34-35 5.689131152188303 1101 36-37 5.497322564877511 1101 38-39 5.025477366977739 1101 40-41 4.644755546670687 1101 42-43 4.35798617339735 1101 44-45 4.25612099891325 1101 46-47 4.354244285054392 1101 48-49 4.2741551499695305 1101 50-51 3.822329860474216 1101 52-53 4.84752005228659 1101 54-55 4.558374275637696 1101 56-57 4.767831075912163 1101 58-59 4.1176582179417345 1101 60-61 4.405644454508074 1101 62-63 3.963338751235675 1101 64-65 3.7344834691101703 1101 66-67 3.345356889960957 1101 68-69 3.50361019974169 1101 70-71 3.33950927006741 1101 72-73 3.067164663547622 1101 74-75 2.6025587397698082 1101 76-77 2.718204234657545 1101 78-79 3.224913090108167 1101 80-81 2.8509992610665087 1101 82-83 2.854097191703403 1101 84-85 2.160052387379693 1101 86-87 2.207694426170491 1101 88-89 2.2928263029541824 1101 90-91 2.1561019162294386 1101 92-93 1.921630933571695 1101 94-95 2.209170456459127 1101 96-97 2.107066582768489 1101 98-99 2.0877926635803057 1101 100-101 1.6610143325877438 2201 1 7.08833144523317 2201 2 7.849341706099146 2201 3 6.360953925699381 2201 4 8.430189499905445 2201 5 3.9013784840102197 2201 6 5.910298863831585 2201 7 6.8404577905865835 2201 8 7.851954094416353 2201 9 6.635579522145424 2201 10-11 4.290934729811699 2201 12-13 6.549360843713668 2201 14-15 7.141301009501312 2201 16-17 6.032528749282136 2201 18-19 1.9925225377764768 2201 20-21 5.0755079899099975 2201 22-23 5.202582461816583 2201 24-25 5.536510775793531 2201 26-27 6.267306498208615 2201 28-29 5.129617461264882 2201 30-31 5.21662278069973 2201 32-33 5.503371626115314 2201 34-35 5.113297522074561 2201 36-37 5.074674177242532 2201 38-39 4.572375898013782 2201 40-41 4.322555367149725 2201 42-43 4.152068628898995 2201 44-45 4.080201955472081 2201 46-47 4.54820132240382 2201 48-49 4.20413817735858 2201 50-51 3.8616817867147795 2201 52-53 4.723913452800147 2201 54-55 4.244020434448153 2201 56-57 4.398534762431055 2201 58-59 3.994399665933699 2201 60-61 3.9859652832979897 2201 62-63 3.7635949912179605 2201 64-65 3.5871866947206534 2201 66-67 3.341616576411173 2201 68-69 3.5066984951773748 2201 70-71 3.375380171251198 2201 72-73 2.6526156296283716 2201 74-75 2.2761203049390417 2201 76-77 2.562777511390564 2201 78-79 3.017625751794789 2201 80-81 2.4045495434444426 2201 82-83 2.6654341078255577 2201 84-85 2.2973735982789663 2201 86-87 2.1318867139005953 2201 88-89 2.21147415308279 2201 90-91 2.0167887732082797 2201 92-93 1.7654281056983159 2201 94-95 2.0888536072413295 2201 96-97 1.9371366522921392 2201 98-99 1.8987998563047306 2201 100-101 1.4423734929332426 2304 1 1.8228313866750376 2304 2 4.967274436748472 2304 3 5.7886212485889175 2304 4 4.268513287333853 2304 5 6.674999718861788 2304 6 6.36242062012937 2304 7 7.0927231537306525 2304 8 6.532704363504909 2304 9 5.7420527053096855 2304 10-11 6.802285944753507 2304 12-13 6.579597753182792 2304 14-15 6.79956216761385 2304 16-17 5.46919771179936 2304 18-19 5.326550389139207 2304 20-21 5.019111629437294 2304 22-23 4.5686721449892005 2304 24-25 4.772414160453522 2304 26-27 5.744927114017077 2304 28-29 5.02962791807408 2304 30-31 5.039306806599157 2304 32-33 5.047898113910126 2304 34-35 4.813922420992071 2304 36-37 4.788990106727386 2304 38-39 4.3241592719660105 2304 40-41 3.801204932983005 2304 42-43 3.413401300432497 2304 44-45 3.187594222452546 2304 46-47 4.284979314584916 2304 48-49 3.9744266179834256 2304 50-51 3.411061070520951 2304 52-53 4.190092925107727 2304 54-55 4.029752606285598 2304 56-57 4.6519576246409855 2304 58-59 3.7419685275078542 2304 60-61 3.7071408796149647 2304 62-63 3.549801762768748 2304 64-65 3.5383139387519087 2304 66-67 3.3990998873436986 2304 68-69 3.6269617000360235 2304 70-71 3.53127948472682 2304 72-73 3.0184323203689374 2304 74-75 2.416261206957344 2304 76-77 2.7436310937539687 2304 78-79 3.2014299668648647 2304 80-81 2.802425556360692 2304 82-83 2.937966022007224 2304 84-85 2.593528559825792 2304 86-87 2.33708095958821 2304 88-89 2.638947537017785 2304 90-91 2.6511036988394565 2304 92-93 2.391125086887211 2304 94-95 2.7457829861347456 2304 96-97 2.5301378401856667 2304 98-99 2.208058175742128 2304 100-101 1.709181768033769 1102 1 7.160548371103644 1102 2 7.575598815230233 1102 3 6.306032263133655 1102 4 6.3975860697904885 1102 5 6.734668020851853 1102 6 5.938009359744967 1102 7 7.043594415692148 1102 8 6.658172966377986 1102 9 5.02574518617207 1102 10-11 6.107225891843861 1102 12-13 6.025082420673048 1102 14-15 6.421157636123951 1102 16-17 5.452136245698171 1102 18-19 5.530002001244412 1102 20-21 5.658917864487844 1102 22-23 5.369677149913727 1102 24-25 1.754102274956054 1102 26-27 5.555976563351688 1102 28-29 5.232020718837273 1102 30-31 4.9393843079404505 1102 32-33 5.010894412399683 1102 34-35 5.043673968313115 1102 36-37 5.066756576052317 1102 38-39 4.0679422728488674 1102 40-41 4.040933048227693 1102 42-43 4.223953079059285 1102 44-45 -3.7375884565195 1102 46-47 1.4731529964436483 1102 48-49 -0.836771881312302 1102 50-51 2.555079551788431 1102 52-53 -0.14735580031410223 1102 54-55 2.8001944837584887 1102 56-57 -0.6593007111017855 1102 58-59 2.997451177257453 1102 60-61 3.937867900177366 1102 62-63 3.750517828777472 1102 64-65 3.222050910409223 1102 66-67 3.1408431147701315 1102 68-69 3.569802354185999 1102 70-71 3.3525050727706613 1102 72-73 2.8105291549126576 1102 74-75 2.5469005601670993 1102 76-77 2.856246186144844 1102 78-79 -1.3136735177191312 1102 80-81 1.7550100038321794 1102 82-83 2.68034773679301 1102 84-85 2.40625323640748 1102 86-87 2.3018267783811837 1102 88-89 2.179998277397182 1102 90-91 2.0665170728265245 1102 92-93 2.0785060131306743 1102 94-95 2.472477965593992 1102 96-97 2.3745593670819147 1102 98-99 2.380719026780298 1102 100-101 2.02748364213603 2202 1 -20.807050270129697 2202 2 -7.8156139889284155 2202 3 -0.6799415988200863 2202 4 1.7256568382274047 2202 5 1.1141060185450122 2202 6 1.8187703601329588 2202 7 4.073072878791919 2202 8 2.71608551227067 2202 9 3.4423268969300302 2202 10-11 2.041246370012008 2202 12-13 1.361786317823281 2202 14-15 2.5726299385181584 2202 16-17 1.7173086286602768 2202 18-19 1.692948093998453 2202 20-21 1.1445122704629362 2202 22-23 1.4857564495735076 2202 24-25 2.531682509625721 2202 26-27 3.727593272644775 2202 28-29 -1.0519259714124978 2202 30-31 0.9543761389761798 2202 32-33 1.7414824409079124 2202 34-35 1.7858844600214212 2202 36-37 3.181127643336076 2202 38-39 2.6049615626240694 2202 40-41 2.068431749969438 2202 42-43 0.9099759597475376 2202 44-45 0.8005466012703124 2202 46-47 -1.23782621855716 2202 48-49 0.8735590293754498 2202 50-51 1.8529042433737395 2202 52-53 1.8748969565463725 2202 54-55 -0.6427664078199555 2202 56-57 1.240784129332873 2202 58-59 1.9126330125954176 2202 60-61 2.102199640298725 2202 62-63 -2.277878889911907 2202 64-65 0.05420995512869453 2202 66-67 0.6303655767824665 2202 68-69 1.5169167236448935 2202 70-71 -2.563891343376703 2202 72-73 -4.5529888065810376 2202 74-75 -1.1545340234532695 2202 76-77 -0.14230836742587627 2202 78-79 1.7102982353485174 2202 80-81 1.6423409828145807 2202 82-83 -0.612578999210875 2202 84-85 -0.32736771804163567 2202 86-87 0.7639235347480913 2202 88-89 1.2632459949700916 2202 90-91 1.338776469444916 2202 92-93 1.313890462537202 2202 94-95 1.5921538695248607 2202 96-97 1.1681673587440287 2202 98-99 1.1576672472261968 2202 100-101 1.1352994537668408 2305 1 5.691915145182158 2305 2 7.208557951432926 2305 3 6.6843139480766105 2305 4 7.678970551027888 2305 5 7.772515228886679 2305 6 6.18318210244523 2305 7 2.798534056486691 2305 8 5.953763802712782 2305 9 6.3755512107152335 2305 10-11 4.7731013426619455 2305 12-13 5.021101302953927 2305 14-15 5.459312450528593 2305 16-17 4.82267493956715 2305 18-19 4.304346216518354 2305 20-21 5.166886703904655 2305 22-23 4.9742907356802135 2305 24-25 5.148500911647716 2305 26-27 6.004616572447414 2305 28-29 5.025733708075254 2305 30-31 5.237611985469492 2305 32-33 5.3524427217386865 2305 34-35 5.193259412248395 2305 36-37 4.547697802883068 2305 38-39 4.401397927827986 2305 40-41 3.922865227144378 2305 42-43 3.9355431618453736 2305 44-45 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38-39 -26.40971646767896 1209 40-41 -23.073627079854223 1209 42-43 -23.632790154787642 1209 44-45 -26.46492057044686 1209 46-47 -20.091369597259433 1209 48-49 -19.75289887594898 1209 50-51 -21.9758146189786 1209 52-53 -24.505469205069787 1209 54-55 -21.393646501125897 1209 56-57 -25.342864860566117 1209 58-59 -25.603149815687413 1209 60-61 -25.354397581923497 1209 62-63 -25.45212131415433 1209 64-65 -25.31479257012383 1209 66-67 -13.151494335903717 1209 68-69 -24.959724690496518 1209 70-71 -24.75880927266963 1209 72-73 -15.227752866673487 1209 74-75 -16.40167706402851 1209 76-77 -13.383889967323155 1209 78-79 -14.1274863905717 1209 80-81 -23.70866911819552 1209 82-83 -15.64920051093832 1209 84-85 -12.861215197069203 1209 86-87 -13.27236894513549 1209 88-89 -10.655154107752047 1209 90-91 -13.248523971404254 1209 92-93 -16.949270811217293 1209 94-95 -22.11649512037413 1209 96-97 -17.927099397804504 1209 98-99 -9.785292541337403 1209 100-101 -5.006061186534481 1212 1 -20.807050270129697 1212 2 -20.61201550407993 1212 3 -21.50191129578978 1212 4 -23.539179020358453 1212 5 -23.693343476404483 1212 6 -24.758881155018557 1212 7 -24.321182171713133 1212 8 -23.98593468974953 1212 9 -24.882799365696233 1212 10-11 -24.608217013826376 1212 12-13 -24.884899288237325 1212 14-15 -25.222035465522247 1212 16-17 -26.259648442046796 1212 18-19 -20.81152275877337 1212 20-21 -26.6731960628704 1212 22-23 -26.86209708578003 1212 24-25 -22.90910846180845 1212 26-27 -25.578593596042094 1212 28-29 -20.450277333507792 1212 30-31 -15.416454217291069 1212 32-33 -25.69396579141532 1212 34-35 -21.860064234622662 1212 36-37 -26.14163119504776 1212 38-39 -26.40971646767896 1212 40-41 -20.878473808738306 1212 42-43 -17.11241179610502 1212 44-45 -13.624421263928136 1212 46-47 -17.26106681628198 1212 48-49 -20.249309522649654 1212 50-51 -12.307767356466552 1212 52-53 -4.690628705763267 1212 54-55 -3.5888129954017387 1212 56-57 -3.5661658314399034 1212 58-59 -2.2577961402366498 1212 60-61 -13.94732407290824 1212 62-63 -11.78360536408498 1212 64-65 -16.141422251122442 1212 66-67 -12.133434437927743 1212 68-69 -7.5540381440332744 1212 70-71 -14.948129661019145 1212 72-73 -11.421646999116657 1212 74-75 -7.867847813223371 1212 76-77 -18.830851872868653 1212 78-79 -9.63047776319447 1212 80-81 -7.169140685463201 1212 82-83 -4.824485249648681 1212 84-85 -3.944315410990548 1212 86-87 -2.9582542503847904 1212 88-89 -2.6688900324329303 1212 90-91 -2.6447912202817534 1212 92-93 -2.515799467274089 1212 94-95 -2.786398032995489 1212 96-97 -12.577977121775449 1212 98-99 -9.670779976396997 1212 100-101 -5.605415602482196 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 332.0 3 746.0 4 355.0 5 2086.0 6 2807.0 7 2619.0 8 5709.0 9 10703.0 10 13259.0 11 15195.0 12 19533.0 13 24832.0 14 28341.0 15 30328.0 16 32707.0 17 36173.0 18 40996.0 19 45849.0 20 50441.0 21 56982.0 22 63523.0 23 69089.0 24 72186.0 25 75104.0 26 79678.0 27 82349.0 28 84228.0 29 85315.0 30 87947.0 31 92268.0 32 100833.0 33 114238.0 34 127837.0 35 138083.0 36 142815.0 37 128436.0 38 46975.0 39 155.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 28.75235865023582 23.729482419123105 26.079424237637372 21.438734693003695 2 28.53738538895479 33.081352299462594 17.362327806254914 21.018934505327696 3 18.70284283432185 28.21879266600704 28.323595580147142 24.754768919523965 4 23.141908215631723 27.27636976643909 27.76723817855381 21.814483839375377 5 22.752759792755445 26.559069474800744 27.44041334714671 23.2477573852971 6 22.630628543751676 28.167278240772426 25.608248616719404 23.593844598756494 7 23.061175450274405 28.358076214518707 26.99287681221465 21.587871522992234 8 21.981167102423235 27.45532778310256 27.164071089276725 23.399434025197476 9 22.351727852323883 26.984577019516664 27.528855507973933 23.134839620185524 10-11 23.105626168308877 27.030451955040053 26.51887806149161 23.345043815159464 12-13 22.91079189372804 27.39445281927244 26.47020346752098 23.224551819478542 14-15 23.08110811877629 27.305125502719875 26.44539951616197 23.168366862341866 16-17 23.163711926091253 27.448963726860043 26.27810600651775 23.109218340530955 18-19 23.158767448623998 27.72659960064579 26.280327951403343 22.834304999326875 20-21 23.358155369027894 27.39248949369416 26.20446283012297 23.04489230715497 22-23 23.01642119414813 27.253488084060397 26.511751486119866 23.218339235671607 24-25 22.997552367618592 27.341545089227886 26.702441078527045 22.958461464626474 26-27 23.125336926171293 27.30162060349578 26.38354152583337 23.189500944499557 28-29 23.119357528228658 27.35229532150047 26.579132529424975 22.949214620845897 30-31 23.129099992464468 27.22377175885476 26.545217277961413 23.10191097071936 32-33 23.115370168347386 27.443275925237938 26.325316192122806 23.116037714291878 34-35 23.081357845201747 27.257001695542165 26.606176505456887 23.055463953799205 36-37 23.194997332694996 27.314564512694805 26.491462697668876 22.998975456941324 38-39 23.130180001341902 27.18879886271398 26.713483528674047 22.967537607270074 40-41 23.302103898051502 27.097745934948097 26.466581551903484 23.133568615096916 42-43 23.053961090723966 27.595885430575517 26.499755454269945 22.850398024430568 44-45 23.146894036580477 27.098437629955967 26.676050800339745 23.078617533123808 46-47 23.22496489274127 27.262904944070502 26.42721599438284 23.084914168805383 48-49 23.009009413011334 27.269843773185805 26.751993365065264 22.969153448737597 50-51 23.427182329598523 27.081550626843182 26.38701196791343 23.104255075644865 52-53 23.06955374986 27.311852592106334 26.61927715085291 22.999316507180755 54-55 23.13838157419284 27.164478415397614 26.74462507139458 22.952514939014964 56-57 23.213466642811927 27.20740395721757 26.533524441563404 23.045604958407097 58-59 23.29920334149771 27.238447643029062 26.542496034902964 22.919852980570266 60-61 23.36696536769892 27.08242312089269 26.519924445220262 23.030687066188126 62-63 23.402811691898773 27.221853058675656 26.43852025907275 22.936814990352822 64-65 23.547680820352454 27.099925422962123 26.503460238550815 22.84893351813461 66-67 23.681581389602798 27.205516977846113 26.269465335204266 22.84343629734682 68-69 23.59170889166768 27.15588170114025 26.45271384836303 22.799695558829043 70-71 23.852144378793362 27.03954640459826 26.25967181156799 22.848637405040396 72-73 23.736550310052056 27.319343665084055 26.307867259896284 22.6362387649676 74-75 24.020154182883978 27.045521632843233 26.164871251268725 22.769452933004068 76-77 24.061664023349454 27.179712513336284 26.060701288002004 22.697922175312257 78-79 24.046619578717586 27.285576283732077 26.180682231617507 22.48712190593283 80-81 24.454827223240354 27.035736880679313 25.906152502993095 22.603283393087235 82-83 24.246875624875024 27.259848030393925 26.015721855628875 22.477554489102182 84-85 24.62410350224698 27.08061992475975 25.909671390516625 22.38560518247665 86-87 24.71114896311288 27.143311528058323 25.709160903417335 22.436378605411463 88-89 24.72809405684986 27.144560226398134 25.852744166672263 22.27460155007974 90-91 25.119915907686586 27.112205952507452 25.4614662751052 22.306411864700763 92-93 25.240656548423463 27.114253393101666 25.412858294948666 22.232231763526205 94-95 25.47280095599314 26.977006406592835 25.361844603154935 22.188348034259086 96-97 25.695041870353023 27.163754971893837 25.145098062705323 21.99610509504781 98-99 25.85467422103365 27.099637084866213 25.10217328619193 21.9435154079082 100-101 26.47039839387415 26.882097732737847 24.721149707785678 21.926354165602323 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 127.0 1 138.0 2 157.5 3 168.5 4 174.5 5 206.0 6 251.5 7 302.5 8 373.5 9 465.5 10 628.5 11 864.0 12 1100.0 13 1432.0 14 1902.5 15 2467.0 16 3067.0 17 3792.0 18 4761.0 19 5968.5 20 7524.0 21 9363.5 22 11521.5 23 13980.5 24 16639.0 25 19750.5 26 23025.5 27 26560.5 28 30375.5 29 33870.5 30 37446.5 31 40996.0 32 44387.0 33 47818.5 34 51023.5 35 54158.0 36 56854.5 37 58822.5 38 60140.5 39 60439.5 40 59634.0 41 57953.0 42 55618.0 43 53120.5 44 50469.5 45 47940.5 46 46312.5 47 44856.0 48 43277.5 49 41995.5 50 40686.5 51 39443.5 52 38651.5 53 38282.0 54 38431.5 55 39040.0 56 39667.5 57 40076.5 58 40215.5 59 40140.5 60 39617.0 61 38239.5 62 36452.0 63 34433.0 64 31758.0 65 28754.5 66 25972.5 67 23230.5 68 20410.0 69 17717.5 70 15141.5 71 12748.0 72 10710.5 73 8946.5 74 7365.5 75 6032.0 76 4885.0 77 4014.5 78 3231.0 79 2546.0 80 2066.0 81 1639.5 82 1328.5 83 1060.0 84 843.0 85 703.0 86 533.0 87 417.5 88 362.0 89 290.0 90 213.5 91 164.0 92 130.0 93 94.0 94 75.0 95 56.0 96 37.0 97 24.0 98 12.5 99 3.0 100 1.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 14.064231059167042 2 11.42193240154904 3 10.041610062793007 4 12.010380636602136 5 9.429343448105767 6 5.548439324293952 7 9.983182931122617 8 10.402764324343677 9 5.643513941956748 10-11 6.590356688936934 12-13 5.544883971175285 14-15 7.597292362405349 16-17 2.7734737838703323 18-19 3.2276639291276408 20-21 3.1886793578684194 22-23 3.2427306703158347 24-25 3.64291922834417 26-27 4.484891489628314 28-29 1.8021165041978031 30-31 2.3382786720582063 32-33 3.163543259945541 34-35 2.0623782975278613 36-37 3.198848165039989 38-39 1.4315393137522054 40-41 1.1899742025566717 42-43 0.5681603459283997 44-45 1.3528740181755619 46-47 1.4199533378550133 48-49 1.3130192555935898 50-51 1.589640645791357 52-53 2.5491881860837013 54-55 1.8827459459029403 56-57 1.8895085756111727 58-59 1.6179591576945798 60-61 1.437754966057566 62-63 0.6235542392737732 64-65 1.2857201106684462 66-67 0.5816110175171999 68-69 0.8243446713461413 70-71 1.030903228757884 72-73 0.08512957397421847 74-75 0.1063622422493302 76-77 0.4705746047342386 78-79 0.9113140784027465 80-81 0.29947012807227263 82-83 0.529673026853607 84-85 0.06745225881777298 86-87 0.10233449955545655 88-89 0.08311570262728164 90-91 0.09107173757814319 92-93 0.17518194457428252 94-95 0.46562694549917155 96-97 0.38116866197393207 98-99 0.06730308316244434 100-101 0.23537432149939433 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2011052.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.5715338122935 #Duplication Level Percentage of deduplicated Percentage of total 1 98.89236367896757 97.47971970158976 2 0.8893785713021383 1.75334819826076 3 0.1485250203163999 0.43921017186268774 4 0.04783521532082693 0.18860762177660925 5 0.010225145869121238 0.050395415588685856 6 0.004268946926406356 0.02524779877794903 7 0.0 0.0 8 0.004821659339399933 0.03802226852840169 9 0.0 0.0 >10 0.0025817619581341112 0.025448823615150964 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 4.972521844288462E-5 0.0 0.0 2 4.972521844288462E-5 0.0 0.0 3 4.972521844288462E-5 0.0 0.0 4 9.945043688576924E-5 0.0 0.0 5 1.4917565532865387E-4 0.0 0.0 6 1.4917565532865387E-4 0.0 0.0 7 1.9890087377153848E-4 0.0 0.0 8 2.486260922144231E-4 0.0 0.0 9 3.480765291001923E-4 0.0 0.0 10-11 5.221147936502885E-4 0.0 0.0 12-13 6.464278397575001E-4 0.0 4.972521844288462E-5 14-15 6.712904489789423E-4 0.0 4.972521844288462E-5 16-17 6.961530582003846E-4 0.0 4.972521844288462E-5 18-19 7.458782766432693E-4 0.0 4.972521844288462E-5 20-21 7.707408858647117E-4 0.0 4.972521844288462E-5 22-23 8.950539319719231E-4 0.0 4.972521844288462E-5 24-25 0.001044229587300577 0.0 7.458782766432692E-5 26-27 0.0013923061164007695 0.0 9.945043688576924E-5 28-29 0.002958650497351635 0.0 9.945043688576924E-5 30-31 0.005991888822367597 0.0 9.945043688576924E-5 32-33 0.011312487195756251 0.0 1.2431304610721156E-4 34-35 0.02001440042326106 0.0 1.9890087377153848E-4 36-37 0.03172468936656039 0.0 1.9890087377153848E-4 38-39 0.048606401027919716 0.0 1.9890087377153848E-4 40-41 0.07195239108685406 0.0 1.9890087377153848E-4 42-43 0.1052434248343653 0.0 2.237634829929808E-4 44-45 0.1482557387874605 2.486260922144231E-5 2.486260922144231E-4 46-47 0.2022076007979903 4.972521844288462E-5 2.486260922144231E-4 48-49 0.2720715327102432 4.972521844288462E-5 2.486260922144231E-4 50-51 0.3518307830926301 4.972521844288462E-5 2.486260922144231E-4 52-53 0.45242490000258573 4.972521844288462E-5 2.486260922144231E-4 54-55 0.5713924851271872 4.972521844288462E-5 2.486260922144231E-4 56-57 0.7122143037574364 4.972521844288462E-5 2.9835131065730774E-4 58-59 0.8713847279931101 4.972521844288462E-5 2.9835131065730774E-4 60-61 1.0505695526520449 4.972521844288462E-5 2.9835131065730774E-4 62-63 1.257376736156002 4.972521844288462E-5 3.2321391987875003E-4 64-65 1.4871569705805718 4.972521844288462E-5 3.480765291001923E-4 66-67 1.7405318211562903 4.972521844288462E-5 3.480765291001923E-4 68-69 2.0231948253948677 4.972521844288462E-5 3.480765291001923E-4 70-71 2.3241069848019844 9.945043688576924E-5 3.480765291001923E-4 72-73 2.6228809598160563 9.945043688576924E-5 3.480765291001923E-4 74-75 2.959843902594264 9.945043688576924E-5 3.729391383216346E-4 76-77 3.313464793550838 9.945043688576924E-5 3.9780174754307695E-4 78-79 3.7023408643834173 9.945043688576924E-5 3.9780174754307695E-4 80-81 4.122270334133578 9.945043688576924E-5 3.9780174754307695E-4 82-83 4.560772172972156 9.945043688576924E-5 3.9780174754307695E-4 84-85 5.026548294126656 9.945043688576924E-5 3.9780174754307695E-4 86-87 5.525913800339325 9.945043688576924E-5 4.475269659859616E-4 88-89 6.0424593695240105 9.945043688576924E-5 4.475269659859616E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACTGCG 435 0.002089971 10.426068 1 GGTTCGT 480 0.007976154 8.864931 8 TACCTTC 805 6.220104E-4 7.7466974 1 TCAGGAC 865 0.0030272137 6.6724615 5 CGAGACC 1040 0.00920785 5.996242 1 AGGGTAG 855 3.2066418E-7 5.8266215 94-95 GGTGACG 1290 0.0022621246 5.7131214 1 TAGGGAA 3505 0.0 5.2976184 92-93 GGGAAAG 4410 0.0 5.134778 94-95 TGTAGGG 5225 0.0 5.104712 90-91 >>END_MODULE