FastQCFastQC Report
Thu 11 Sep 2014
NYCLib5_CAGATC_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNYCLib5_CAGATC_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2011052
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTGCG4350.00208997110.4260681
GGTTCGT4800.0079761548.8649318
TACCTTC8056.220104E-47.74669741
TCAGGAC8650.00302721376.67246155
CGAGACC10400.009207855.9962421
AGGGTAG8553.2066418E-75.826621594-95
GGTGACG12900.00226212465.71312141
TAGGGAA35050.05.297618492-93
GGGAAAG44100.05.13477894-95
TGTAGGG52250.05.10471290-91
Produced by FastQC (version 0.11.2)