##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename NYCLib5_CAGATC_L007_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2011052 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.25244349723428 33.0 33.0 33.0 27.0 33.0 2 31.649246762391027 33.0 33.0 33.0 27.0 33.0 3 31.789461436104087 33.0 33.0 33.0 27.0 33.0 4 35.625046493079246 37.0 37.0 37.0 33.0 37.0 5 35.12960231759298 37.0 37.0 37.0 33.0 37.0 6 34.922000525098305 37.0 33.0 37.0 33.0 37.0 7 34.70861171168125 37.0 33.0 37.0 27.0 37.0 8 34.75964917863884 37.0 33.0 37.0 27.0 37.0 9 34.668982701591005 37.0 33.0 37.0 27.0 37.0 10-11 34.509639482221246 37.0 33.0 37.0 27.0 37.0 12-13 34.503510849048155 37.0 33.0 37.0 27.0 37.0 14-15 35.42123251909946 37.0 33.0 40.0 27.0 40.0 16-17 35.315671847371426 37.0 33.0 40.0 27.0 40.0 18-19 35.53583795943615 37.0 33.0 40.0 27.0 40.0 20-21 35.20849535467009 37.0 33.0 40.0 27.0 40.0 22-23 35.23480994027007 37.0 33.0 40.0 27.0 40.0 24-25 35.07287902053254 37.0 33.0 40.0 27.0 40.0 26-27 34.68123623854579 37.0 33.0 40.0 27.0 40.0 28-29 34.302549859476535 37.0 33.0 40.0 27.0 40.0 30-31 34.18867438534657 37.0 33.0 40.0 24.5 40.0 32-33 34.19677213717 37.0 33.0 38.5 24.5 40.0 34-35 34.03404884607658 37.0 33.0 37.0 24.5 40.0 36-37 33.72937969779001 37.0 33.0 37.0 22.0 40.0 38-39 33.63427748263098 37.0 33.0 37.0 22.0 40.0 40-41 33.33380688316364 37.0 33.0 37.0 22.0 40.0 42-43 32.93316408526483 37.0 33.0 37.0 22.0 40.0 44-45 32.8164679481187 37.0 33.0 37.0 22.0 40.0 46-47 32.65596787154186 37.0 33.0 37.0 22.0 40.0 48-49 32.66250425150618 37.0 33.0 37.0 22.0 40.0 50-51 32.957840722169294 37.0 33.0 37.0 22.0 40.0 52-53 32.788833157969066 37.0 33.0 37.0 22.0 40.0 54-55 32.41152391882457 37.0 33.0 37.0 22.0 40.0 56-57 32.4473820169742 37.0 33.0 37.0 22.0 40.0 58-59 32.142625600929264 35.0 27.0 37.0 22.0 40.0 60-61 32.04654827423657 33.0 27.0 37.0 22.0 40.0 62-63 32.02759824211408 33.0 27.0 37.0 22.0 40.0 64-65 31.679133607683937 33.0 27.0 37.0 18.5 40.0 66-67 31.32182086788407 33.0 27.0 37.0 15.0 40.0 68-69 31.154597195895484 33.0 27.0 37.0 15.0 40.0 70-71 30.99166456163242 33.0 27.0 37.0 15.0 37.0 72-73 30.772181674069095 33.0 27.0 37.0 15.0 37.0 74-75 30.422456505351427 33.0 27.0 37.0 15.0 37.0 76-77 29.32466017785716 33.0 27.0 35.0 15.0 37.0 78-79 30.25308544980438 33.0 27.0 37.0 15.0 37.0 80-81 29.97209296427939 33.0 27.0 37.0 15.0 37.0 82-83 29.956433249861266 33.0 27.0 37.0 15.0 37.0 84-85 29.832545602997833 33.0 27.0 37.0 15.0 37.0 86-87 29.636214528515424 33.0 27.0 37.0 15.0 37.0 88-89 29.50242957417312 33.0 27.0 37.0 15.0 37.0 90-91 29.41236974479029 33.0 27.0 33.0 15.0 37.0 92-93 29.20339329863176 33.0 27.0 33.0 15.0 37.0 94-95 29.1118877085227 33.0 27.0 33.0 15.0 37.0 96-97 28.961379168713687 33.0 27.0 33.0 15.0 37.0 98-99 28.700068919152763 33.0 27.0 33.0 15.0 37.0 100-101 27.87270965643852 33.0 24.5 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 1.1180314779979845 2306 2 0.5630440468870397 2306 3 0.5181532869638374 2306 4 0.37107665190869454 2306 5 0.6701478535390848 2306 6 0.8140090140117948 2306 7 0.8967676222458607 2306 8 0.8059841522421394 2306 9 0.9502061926547185 2306 10-11 1.1188787831874265 2306 12-13 1.3783071190610912 2306 14-15 1.5467215953988216 2306 16-17 1.4481952412381034 2306 18-19 1.4016208279133977 2306 20-21 1.5949948794216198 2306 22-23 1.2978524778178908 2306 24-25 1.2768064551919451 2306 26-27 1.444305650674842 2306 28-29 1.6288920983705282 2306 30-31 1.5704503325057715 2306 32-33 1.2536547652179308 2306 34-35 1.310458693494681 2306 36-37 1.355221056426977 2306 38-39 1.2015744693176842 2306 40-41 1.3834053194818878 2306 42-43 1.3150138903537325 2306 44-45 1.1285642366669748 2306 46-47 0.9856441389638348 2306 48-49 0.9492943834378949 2306 50-51 0.9894185082214548 2306 52-53 1.1602056787729325 2306 54-55 1.635229761011356 2306 56-57 1.2730470052221534 2306 58-59 1.157606928157069 2306 60-61 0.9440858924167586 2306 62-63 0.7855459268025307 2306 64-65 0.7629441236146768 2306 66-67 0.9447430781690791 2306 68-69 0.8132749797326113 2306 70-71 0.8215793388769796 2306 72-73 0.7067327124662484 2306 74-75 0.8625012864272215 2306 76-77 0.9420279280045953 2306 78-79 0.9728114936322392 2306 80-81 1.2492682091781298 2306 82-83 1.1483735227221707 2306 84-85 0.9059238288896729 2306 86-87 0.846854825112807 2306 88-89 0.5374418668260255 2306 90-91 0.3138864368562473 2306 92-93 0.0981851105474334 2306 94-95 0.0634670235901531 2306 96-97 0.07297963527472362 2306 98-99 0.2453559239809877 2306 100-101 0.1481539823254252 2307 1 0.44962745368482615 2307 2 0.4250536022541631 2307 3 0.3578747261210893 2307 4 0.2784026296036757 2307 5 0.5143826577602439 2307 6 0.5956797009651496 2307 7 0.8017628112022379 2307 8 0.7230489228655728 2307 9 1.037595589345706 2307 10-11 1.1198654941103143 2307 12-13 1.2507631148555447 2307 14-15 1.2348376798961453 2307 16-17 1.2055470729778293 2307 18-19 1.0622619340892996 2307 20-21 1.3668980467301637 2307 22-23 1.1299157123523997 2307 24-25 1.2094627737722092 2307 26-27 1.446920708532737 2307 28-29 1.4727495305662046 2307 30-31 1.4902091458296454 2307 32-33 1.0765511791861542 2307 34-35 1.0891761431815041 2307 36-37 1.1839581514811144 2307 38-39 1.1088928156269162 2307 40-41 1.1095052910713292 2307 42-43 1.1589581568172989 2307 44-45 0.8450345441442266 2307 46-47 0.8976875395267712 2307 48-49 0.9784099734214138 2307 50-51 0.9488804508277653 2307 52-53 1.3050083889178552 2307 54-55 1.4858697780795005 2307 56-57 1.1297604606416698 2307 58-59 1.0664884820729164 2307 60-61 0.9823756072929228 2307 62-63 0.896579264402174 2307 64-65 0.8432639953289076 2307 66-67 1.054909544857093 2307 68-69 0.8800486784014581 2307 70-71 0.6527054664959877 2307 72-73 0.5951580370672431 2307 74-75 0.8750172769058686 2307 76-77 0.8725153794989602 2307 78-79 0.9092726576191623 2307 80-81 1.4824347894647794 2307 82-83 1.2477823247188908 2307 84-85 0.9688709599574175 2307 86-87 0.946792781946634 2307 88-89 0.7952856503973109 2307 90-91 0.5509975714187298 2307 92-93 0.6202349912470702 2307 94-95 0.5289870327565396 2307 96-97 0.5102136405988169 2307 98-99 0.6925890887624462 2307 100-101 0.6634442813296602 1101 1 0.9962695324488919 1101 2 0.5622421457910107 1101 3 0.41899845056748575 1101 4 0.39173705586360796 1101 5 0.5234121168455346 1101 6 0.5724899563874004 1101 7 0.7106022380432506 1101 8 0.6200496126466106 1101 9 0.8621327047353375 1101 10-11 0.989653140092237 1101 12-13 1.144005359679646 1101 14-15 1.3144181838825943 1101 16-17 1.3519033300783505 1101 18-19 1.001230894826577 1101 20-21 1.3803883255015634 1101 22-23 1.0004087710164882 1101 24-25 0.9992785721882314 1101 26-27 1.0775256213114517 1101 28-29 1.2063707661005765 1101 30-31 1.2815291728552722 1101 32-33 0.8317719290015617 1101 34-35 0.9509110595792407 1101 36-37 1.1302637134317095 1101 38-39 1.001116849082841 1101 40-41 0.9703188791832389 1101 42-43 0.9750441288981762 1101 44-45 0.8331730500546541 1101 46-47 0.722889745677179 1101 48-49 0.8527299690732804 1101 50-51 0.8985352178510198 1101 52-53 0.9429687934538791 1101 54-55 1.3535548291855939 1101 56-57 1.0461292053860092 1101 58-59 1.1671015630786634 1101 60-61 0.8134913822448979 1101 62-63 0.6369700534171123 1101 64-65 0.6756380904055668 1101 66-67 0.7016222366955418 1101 68-69 0.5949289477032877 1101 70-71 0.7782743922360424 1101 72-73 0.5585068466536498 1101 74-75 0.6818767648417712 1101 76-77 0.7482306241016801 1101 78-79 0.9873399296080869 1101 80-81 1.5496345303439583 1101 82-83 1.1602062598998018 1101 84-85 1.072066632409964 1101 86-87 0.7945599638353968 1101 88-89 0.66011822936089 1101 90-91 0.5322525177585398 1101 92-93 0.6646753273971164 1101 94-95 0.6465719971207982 1101 96-97 0.49157590953211283 1101 98-99 0.6277999359558422 1101 100-101 0.5236529301498081 2201 1 1.4210535284059311 2201 2 0.7553022386506321 2201 3 0.2647834277707872 2201 4 0.24135149079298657 2201 5 0.34336399219688474 2201 6 0.4776971208498253 2201 7 0.5525325550311351 2201 8 0.6286468530950842 2201 9 0.567261378300806 2201 10-11 0.7919685555195102 2201 12-13 0.9437798909463666 2201 14-15 1.011093568336122 2201 16-17 0.8893584663456764 2201 18-19 0.7751796978954317 2201 20-21 1.1301640343518429 2201 22-23 0.920749920276414 2201 24-25 0.8764473619536872 2201 26-27 0.9295977941974485 2201 28-29 1.319342795519482 2201 30-31 1.4445626457230887 2201 32-33 0.8883538287277872 2201 34-35 0.8832347819230932 2201 36-37 0.96311166956486 2201 38-39 0.7056745597019187 2201 40-41 0.7903970971449326 2201 42-43 0.8287389459016907 2201 44-45 0.7176056044513075 2201 46-47 0.6465716004571362 2201 48-49 0.7627216445479803 2201 50-51 0.7010261702673972 2201 52-53 0.879060398009436 2201 54-55 1.3334578249141593 2201 56-57 0.8725749588436003 2201 58-59 0.9968922041287449 2201 60-61 0.8502256612842203 2201 62-63 0.6746042484187846 2201 64-65 0.7805594102148099 2201 66-67 0.6968859295711844 2201 68-69 0.6447399070222879 2201 70-71 0.9092985575714181 2201 72-73 0.7923425312655183 2201 74-75 0.7434570665760951 2201 76-77 0.7941956725410861 2201 78-79 0.7104845861740934 2201 80-81 1.1555156908118391 2201 82-83 0.9403024343600173 2201 84-85 0.9434369786987737 2201 86-87 0.9081858822955482 2201 88-89 0.940756422423842 2201 90-91 0.7313163608366118 2201 92-93 0.9542607893686501 2201 94-95 0.9086034894471346 2201 96-97 0.6700693487408458 2201 98-99 0.7847940559619531 2201 100-101 0.7781361118353232 2304 1 -1.8662926170747767 2304 2 -0.27509026813932635 2304 3 -0.16632293052689917 2304 4 -0.01272554940020143 2304 5 0.4382200786175332 2304 6 0.5643358283324389 2304 7 0.6620764150716241 2304 8 0.774541928077376 2304 9 0.9849964055442797 2304 10-11 0.9388046039052611 2304 12-13 1.1814881208734747 2304 14-15 1.2501366448130398 2304 16-17 1.1914747851026775 2304 18-19 0.8816351741961128 2304 20-21 1.2691332160437767 2304 22-23 0.8802352221854122 2304 24-25 1.023463546305841 2304 26-27 1.1597839532859453 2304 28-29 1.5297269647468994 2304 30-31 1.5317353475952729 2304 32-33 0.9472927275002974 2304 34-35 0.9388217853640839 2304 36-37 1.3465146220100763 2304 38-39 1.1497770587398861 2304 40-41 0.9765598106729669 2304 42-43 0.8864337632282115 2304 44-45 0.9509062195905358 2304 46-47 0.9469509734947366 2304 48-49 0.8999209764275804 2304 50-51 0.8307914079297589 2304 52-53 1.2061425578564027 2304 54-55 1.4208773074833552 2304 56-57 1.0509880613649472 2304 58-59 0.855964810634827 2304 60-61 0.49916603795243475 2304 62-63 0.42772846415809695 2304 64-65 0.40961539736548147 2304 66-67 0.4229473654165652 2304 68-69 0.5090346440405078 2304 70-71 0.5290376671532009 2304 72-73 0.36332293381084213 2304 74-75 0.4771182324963803 2304 76-77 0.46589062371475976 2304 78-79 0.5237100795960679 2304 80-81 1.1111527475128433 2304 82-83 0.8652576792166613 2304 84-85 0.5691420464867818 2304 86-87 0.5584576199105058 2304 88-89 0.2412821907901801 2304 90-91 0.24686563611002654 2304 92-93 0.2620720258375435 2304 94-95 0.03848737703782845 2304 96-97 0.07762069864520171 2304 98-99 0.5769536798514387 2304 100-101 0.5815322046137119 1102 1 1.462894426710971 1102 2 0.20172821096353033 1102 3 0.2758239609928701 1102 4 0.22639156150441408 1102 5 0.3721219097365065 1102 6 0.23673251769159975 1102 7 -4.850003976045368 1102 8 -1.2034176954632372 1102 9 0.24009518206674585 1102 10-11 0.7081189818149412 1102 12-13 0.865785614700421 1102 14-15 0.8670573329167155 1102 16-17 0.7321456336206111 1102 18-19 0.8993517102379087 1102 20-21 1.1730346838148193 1102 22-23 0.7390261162223268 1102 24-25 1.0787758863747712 1102 26-27 1.1038957840492998 1102 28-29 0.9494583037381545 1102 30-31 1.3403074984449717 1102 32-33 0.912364670391284 1102 34-35 1.1720424550684356 1102 36-37 1.4068033985789299 1102 38-39 0.9831835383931775 1102 40-41 1.2219687935616008 1102 42-43 1.2820405774708306 1102 44-45 0.9989683768258786 1102 46-47 0.9434667760375532 1102 48-49 0.9352607763233678 1102 50-51 0.7070431802172905 1102 52-53 0.80052597187359 1102 54-55 1.3537389971594784 1102 56-57 1.1185425210356428 1102 58-59 0.9775400244303007 1102 60-61 1.016313625244841 1102 62-63 0.797600774417031 1102 64-65 0.5769395562923201 1102 66-67 0.6794088513086187 1102 68-69 0.841136356723517 1102 70-71 0.5967475933549196 1102 72-73 0.5670395966378763 1102 74-75 0.7480567756721648 1102 76-77 0.5563559100715842 1102 78-79 0.6822555205071872 1102 80-81 1.2689566494058049 1102 82-83 1.1905296876648883 1102 84-85 0.7999424324086704 1102 86-87 0.6389785758093431 1102 88-89 0.5125893388490539 1102 90-91 0.2047578624742421 1102 92-93 0.26367632292936705 1102 94-95 0.2832741845701108 1102 96-97 0.178402205936127 1102 98-99 0.18469337739134062 1102 100-101 0.48617416774394684 2202 1 1.515280137094134 2202 2 0.9493251134875926 2202 3 0.433715919511954 2202 4 -0.36475284902365246 2202 5 0.12106293021038539 2202 6 0.4785149718385 2202 7 0.6416696850495143 2202 8 -3.884019275676131 2202 9 -0.5001195468793611 2202 10-11 0.5258843510811602 2202 12-13 0.06636070021720286 2202 14-15 0.12471713910507276 2202 16-17 0.2856984567302021 2202 18-19 0.7965901978049814 2202 20-21 1.1738957078255012 2202 22-23 -2.7256793226714393 2202 24-25 -2.9137527363431346 2202 26-27 -1.4663219653680457 2202 28-29 0.6177873018457021 2202 30-31 1.445606305696998 2202 32-33 1.2195461418210272 2202 34-35 -1.8672623548546738 2202 36-37 0.04461190400159154 2202 38-39 0.375105229500754 2202 40-41 1.315962155123394 2202 42-43 1.4365258452914418 2202 44-45 1.472380765343928 2202 46-47 0.7309460649417474 2202 48-49 1.1458686185290645 2202 50-51 -3.975023023365445 2202 52-53 -0.1513388763077188 2202 54-55 1.5461307666310482 2202 56-57 1.3641154521365593 2202 58-59 1.458821969501603 2202 60-61 1.3451642971718414 2202 62-63 1.1936848101625195 2202 64-65 1.2438589049263022 2202 66-67 1.311367812490861 2202 68-69 1.3190372215911594 2202 70-71 1.3275014096073292 2202 72-73 1.2286797191195546 2202 74-75 0.5536399212776821 2202 76-77 1.3133330584168057 2202 78-79 1.369657161360454 2202 80-81 2.05265523540956 2202 82-83 1.7843853464212494 2202 84-85 1.5035996028193352 2202 86-87 1.568212902021557 2202 88-89 1.49267686982909 2202 90-91 1.3412572220881493 2202 92-93 1.4064996601690218 2202 94-95 1.3576688366039065 2202 96-97 1.1821422454744415 2202 98-99 1.3999566384313198 2202 100-101 1.3179333835629699 2305 1 1.2326462121216757 2305 2 0.6480425382525361 2305 3 0.26482034239881713 2305 4 -0.13380449092299074 2305 5 0.19930946218696022 2305 6 0.2403694947501478 2305 7 0.46974537245863246 2305 8 -0.2465081535175102 2305 9 0.4353775315012527 2305 10-11 0.27383281667113835 2305 12-13 0.7766387784425532 2305 14-15 0.6084549943050135 2305 16-17 0.6279210260533503 2305 18-19 0.6431456503499433 2305 20-21 0.8040060224706238 2305 22-23 0.42886783005594964 2305 24-25 0.7470165270116667 2305 26-27 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-1.6081909993892438 1208 50-51 -0.20044572867945476 1208 52-53 -1.4315005473661309 1208 54-55 -0.8843355816342147 1208 56-57 -6.344400413036908 1208 58-59 -1.9803372860978676 1208 60-61 -1.3350714845585046 1208 62-63 0.42286931828724406 1208 64-65 0.015764098163014495 1208 66-67 0.7660477641816819 1208 68-69 0.5869514179020996 1208 70-71 -4.120759734441858 1208 72-73 -0.3997527029269996 1208 74-75 -5.645785034804881 1208 76-77 -1.237656456295536 1208 78-79 -3.893991554054562 1208 80-81 0.8709482195992813 1208 82-83 0.2371109722447038 1208 84-85 -2.903036047158704 1208 86-87 -1.1048286548515236 1208 88-89 -5.076472504347457 1208 90-91 -0.2126500572267389 1208 92-93 -2.274802491785305 1208 94-95 -4.641334786584501 1208 96-97 -3.473114122553664 1208 98-99 -6.523956405046938 1208 100-101 -3.8240585466083097 1209 1 1.9424276717628537 1209 2 1.3338857623490874 1209 3 0.7046027539581061 1209 4 0.1857575515926797 1209 5 0.4652409391129666 1209 6 1.1387240541874135 1209 7 1.0642000736815973 1209 8 0.607688051836277 1209 9 0.3089884825676492 1209 10-11 -2.5015135687955734 1209 12-13 -0.09549889146230583 1209 14-15 -1.4697497758067612 1209 16-17 -0.42407405653807473 1209 18-19 -16.64933087735338 1209 20-21 -13.867476702720637 1209 22-23 -3.3603148730908927 1209 24-25 -2.037599787361792 1209 26-27 -1.6871772341576445 1209 28-29 -3.365103042273457 1209 30-31 -0.7933724650614309 1209 32-33 -0.23262653837072378 1209 34-35 -3.025117388239373 1209 36-37 -3.6466304988403913 1209 38-39 -1.4210207549197236 1209 40-41 -10.771231283500128 1209 42-43 -5.368128216513043 1209 44-45 -2.4200044212679543 1209 46-47 -2.4830610614064774 1209 48-49 -3.3547412941571153 1209 50-51 -2.7272005186573445 1209 52-53 -0.9854292503875044 1209 54-55 -2.449875375296706 1209 56-57 -0.5828676201113367 1209 58-59 -1.5300471239755389 1209 60-61 -2.028554351176041 1209 62-63 -1.2158742336655912 1209 64-65 -0.31666957906616844 1209 66-67 0.373043895353387 1209 68-69 -1.2511749638222938 1209 70-71 -0.7171211935428161 1209 72-73 -0.3394726734502136 1209 74-75 0.6862986055783189 1209 76-77 -16.159202145315433 1209 78-79 -16.360576852507034 1209 80-81 -21.51145782314206 1209 82-83 -9.917720457379467 1209 84-85 -3.24836121296563 1209 86-87 -1.652797519995218 1209 88-89 -1.514248849225865 1209 90-91 -5.625269806674051 1209 92-93 -3.1745491756467636 1209 94-95 -0.431879185994962 1209 96-97 0.5210503947639467 1209 98-99 1.232583757075389 1209 100-101 0.7507065602450425 1212 1 -28.629606226542233 1212 2 -16.288995593583113 1212 3 -5.638770385984063 1212 4 -1.187414713857848 1212 5 -0.5919036811178771 1212 6 -2.475985388911859 1212 7 -0.18624363679585088 1212 8 -1.2814724832021582 1212 9 0.12645304449905126 1212 10-11 0.8422812430241677 1212 12-13 0.2390749701705559 1212 14-15 -0.00636176715587311 1212 16-17 0.12318698626970104 1212 18-19 -0.7540803425500187 1212 20-21 -0.33916004036374403 1212 22-23 -0.1759899007126009 1212 24-25 0.6481459400883054 1212 26-27 -0.64200104289786 1212 28-29 0.2681335171190966 1212 30-31 -0.5206109991125345 1212 32-33 0.22377817260038313 1212 34-35 -0.7893704853972707 1212 36-37 -1.5445829659881838 1212 38-39 -0.6658313992696137 1212 40-41 -0.2425076416655827 1212 42-43 -0.2211292861197549 1212 44-45 1.104017652593683 1212 46-47 -1.8074692515378885 1212 48-49 -0.8579192602588108 1212 50-51 0.2729302437019001 1212 52-53 -0.40208073490759233 1212 54-55 -0.11685156185492573 1212 56-57 -0.8507864710481243 1212 58-59 0.0631232608477994 1212 60-61 0.4320729555307423 1212 62-63 -4.62900835764594 1212 64-65 -3.623645236697989 1212 66-67 -1.4242906094539656 1212 68-69 0.3057198856099923 1212 70-71 -0.6589833670776919 1212 72-73 -0.9254672901548844 1212 74-75 0.2571909044099776 1212 76-77 1.1792637330550058 1212 78-79 -1.3068673153763974 1212 80-81 0.5969809036733373 1212 82-83 0.9075780921868137 1212 84-85 -3.051721070978278 1212 86-87 -1.352026462847661 1212 88-89 -0.6049391569434874 1212 90-91 0.10026000126689993 1212 92-93 -2.4280871055463855 1212 94-95 -1.641348731588419 1212 96-97 0.4795546496829637 1212 98-99 -0.14572644062554474 1212 100-101 0.3332851939693917 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 7.0 10 11.0 11 9.0 12 33.0 13 195.0 14 579.0 15 1463.0 16 2848.0 17 4672.0 18 7189.0 19 10393.0 20 14746.0 21 19551.0 22 25878.0 23 32782.0 24 41293.0 25 50224.0 26 61721.0 27 73841.0 28 87724.0 29 103003.0 30 119386.0 31 138601.0 32 157662.0 33 178567.0 34 197529.0 35 212297.0 36 216484.0 37 186742.0 38 65435.0 39 186.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 26.843402217792423 22.97348465876649 29.340973063939334 20.842140059501748 2 28.449711933501543 32.52804334572853 18.266787469281358 20.75545725148857 3 18.51357177274149 26.795717061095974 30.43958650435668 24.251124661805854 4 22.98379156779636 26.765643056469944 29.156729910514496 21.0938354652192 5 22.29678794978946 26.381068217032677 28.45227274083415 22.869871092343708 6 22.12260050958404 28.341534679361846 26.479275523457375 23.05658928759674 7 22.3391538359028 28.47057162122113 27.80256303665942 21.387711506216647 8 21.526643766546066 27.382235765161717 27.801419356635233 23.28970111165698 9 21.994756972967384 26.753112301422338 28.101212698627386 23.150918026982893 10-11 22.583304658457365 26.98169913060428 27.132465992923105 23.302530218015246 12-13 22.709560640604572 27.175572366952583 26.891606015433183 23.22326097700966 14-15 22.66796184285638 27.20081330567285 26.825412769038294 23.305812082432478 16-17 22.690361804345052 27.329366739837564 26.807797237804916 23.17247421801247 18-19 22.915087588288372 27.596502691434083 26.62089050686082 22.86751921341672 20-21 22.95274387552979 27.14378522877211 26.712269675838733 23.191201219859362 22-23 22.644820383764415 27.102563012434043 26.980313532497803 23.272303071303742 24-25 22.743991701855546 26.978591304451598 27.149720643722787 23.127696349970066 26-27 23.03575442106917 27.11337150904104 26.63531325893115 23.215560810958642 28-29 22.978744458124403 27.126250340617748 26.91989068407977 22.975114517178074 30-31 23.00281146385076 26.950397105594487 26.890527942589255 23.156263487965504 32-33 23.00748563438439 27.04427831801465 26.729517685271194 23.218718362329767 34-35 23.034262664515886 26.946990928131147 26.866237173379904 23.152509233973063 36-37 23.099651326768278 27.09574391910304 26.793116239659643 23.011488514469043 38-39 23.039260048969396 26.839460143248413 27.04174233187406 23.079537475908133 40-41 23.107743762841793 26.824429690126166 26.846557852464333 23.22126869456771 42-43 22.80885824931429 27.205238850114267 27.021230679266374 22.96467222130507 44-45 23.034411840171217 26.71405314233545 27.031871876013152 23.219663141480183 46-47 22.976253594737432 26.938845446433813 26.973313146766753 23.111587812062005 48-49 22.996817582854728 26.921284900922405 27.105019765793987 22.97687775042888 50-51 23.176790614448134 26.815017141555757 26.778891707484924 23.229300536511175 52-53 22.947725291626412 27.01905821290282 26.99790010089262 23.035316394578153 54-55 22.822015632392873 26.845801613263742 27.299452789174033 23.032729965169356 56-57 23.1469715326832 26.936480914836736 26.75674674438489 23.159800808095174 58-59 22.948573317136894 26.934694351927135 27.118509509435523 22.99822282150045 60-61 23.171460012521027 26.87207517390455 26.863000163598816 23.09346464997561 62-63 23.226781559955356 26.925839577867112 26.773479500881514 23.073899361296014 64-65 23.150476080604516 26.8761772726459 26.9239892113591 23.049357435390483 66-67 23.233740210858915 27.138730127394027 26.63787618435025 22.9896534773968 68-69 23.201817843218233 27.011252616605695 26.704259096861716 23.082670443314356 70-71 23.309907743870827 26.943265153389333 26.61645891115142 23.130368191588417 72-73 23.13081932922029 27.139803185886493 26.761894220808376 22.967483264084844 74-75 23.2434470735166 26.870362756240013 26.73363176855078 23.15255840169261 76-77 23.119144603104917 27.118887607507958 26.629599866322444 23.13236792306468 78-79 23.21205561982561 27.27690646482141 26.500046866169864 23.010991049183126 80-81 23.244742697676084 27.01151041852062 26.413101082140145 23.33064580166315 82-83 23.09448649745345 27.243487419403923 26.43436896119951 23.227657121943118 84-85 23.08485464957989 27.10760409070238 26.582131186222636 23.225410073495095 86-87 23.184290936143263 27.136694704476643 26.246602604634557 23.432411754745537 88-89 23.088521343938478 27.194745862632875 26.3447080315111 23.372024761917547 90-91 23.240973374279783 27.181476514273022 26.151183717643573 23.426366393803622 92-93 23.094405342274257 27.198235155749195 26.13322664433425 23.574132857642297 94-95 23.11924707469102 27.12882947109081 26.111930725062855 23.639992729155317 96-97 23.164764066847518 27.322593238166043 25.945560199816498 23.56708249516994 98-99 23.09488737464107 27.17040161605231 26.026294666967704 23.70841634233892 100-101 23.087636394922015 26.980794182605205 25.955951794238803 23.975617628233977 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 1.5 2 2.5 3 5.0 4 10.5 5 18.5 6 22.5 7 34.5 8 54.5 9 75.5 10 108.5 11 175.0 12 261.0 13 353.5 14 489.0 15 716.0 16 1004.5 17 1479.5 18 2254.0 19 3172.0 20 4488.5 21 6155.0 22 8126.5 23 10482.0 24 13187.0 25 16373.5 26 19770.0 27 23416.5 28 27117.0 29 31195.0 30 35389.0 31 39145.0 32 43055.0 33 46736.5 34 49942.5 35 53303.0 36 56698.5 37 59459.5 38 61177.5 39 61817.0 40 61486.0 41 60378.5 42 58205.5 43 55272.0 44 52583.5 45 50228.0 46 48204.0 47 46538.0 48 44904.5 49 43228.5 50 41610.5 51 40413.0 52 39705.0 53 39597.5 54 40011.0 55 40658.5 56 41569.0 57 42806.5 58 43749.0 59 43703.0 60 43304.5 61 42478.5 62 40594.0 63 38053.0 64 35502.5 65 32253.0 66 28852.5 67 25581.0 68 22038.5 69 18657.5 70 15687.0 71 13056.5 72 10627.5 73 8520.5 74 6603.0 75 5060.5 76 3766.5 77 2682.0 78 1838.0 79 1223.5 80 821.5 81 543.5 82 364.0 83 245.5 84 176.0 85 120.5 86 86.5 87 60.0 88 47.5 89 33.0 90 16.5 91 12.5 92 8.5 93 4.5 94 3.5 95 2.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7196233613054263 2 0.002884062669687308 3 1.4917565532865387E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.19631516241250846 14-15 0.0 16-17 3.2321391987875003E-4 18-19 0.11757527900820067 20-21 2.486260922144231E-5 22-23 2.486260922144231E-5 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0019890087377153844 42-43 0.0 44-45 0.0 46-47 0.023569753541927307 48-49 9.945043688576924E-5 50-51 1.7403826455009616E-4 52-53 1.4917565532865387E-4 54-55 0.001765245254722404 56-57 0.0013177182887364426 58-59 0.09788409250481839 60-61 0.0017403826455009619 62-63 0.0013177182887364426 64-65 0.002386810485258462 66-67 0.0043012313953095195 68-69 0.00484820879818125 70-71 0.0037045287739949045 72-73 0.03876080777622856 74-75 0.0447775592078176 76-77 0.16088594427195319 78-79 3.2321391987875003E-4 80-81 0.11901731034304433 82-83 0.004873071407402693 84-85 0.005047109671952789 86-87 0.00678749231745375 88-89 0.007036118409668174 90-91 0.022799012656062597 92-93 0.01108872371276327 94-95 0.013997648991672018 96-97 0.010293120217677117 98-99 0.011237899368091924 100-101 0.012679930702935577 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2011052.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.6397554272446 #Duplication Level Percentage of deduplicated Percentage of total 1 98.25470305161691 95.93565175536418 2 1.3848217874703468 2.7042732127784865 3 0.22269811379561646 0.6523256809533803 4 0.06436773046231953 0.25139397838990724 5 0.046436968527509136 0.22670471249043234 6 0.015078937531790246 0.08833822636240193 7 0.005550207049284812 0.03793446012039166 8 0.0032440491181511474 0.02533985299921972 9 0.0014446604078567514 0.012695066000870095 >10 0.0016009532930236407 0.025769338982228433 >50 0.0 0.0 >100 0.0 0.0 >500 5.35407271916439E-5 0.03957371555850424 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 4.972521844288462E-5 0.0 0.0 2 9.945043688576924E-5 0.0 0.0 3 1.4917565532865387E-4 0.0 0.0 4 1.9890087377153848E-4 0.0 0.0 5 2.9835131065730774E-4 0.0 0.0 6 3.480765291001923E-4 4.972521844288462E-5 0.0 7 3.9780174754307695E-4 4.972521844288462E-5 0.0 8 4.475269659859616E-4 4.972521844288462E-5 0.0 9 4.972521844288462E-4 4.972521844288462E-5 0.0 10-11 6.215652305360578E-4 4.972521844288462E-5 2.486260922144231E-5 12-13 6.712904489789423E-4 4.972521844288462E-5 4.972521844288462E-5 14-15 7.707408858647117E-4 4.972521844288462E-5 4.972521844288462E-5 16-17 7.956034950861539E-4 7.458782766432692E-5 9.945043688576924E-5 18-19 7.956034950861539E-4 9.945043688576924E-5 1.2431304610721156E-4 20-21 8.204661043075961E-4 9.945043688576924E-5 1.9890087377153848E-4 22-23 9.199165411933655E-4 9.945043688576924E-5 2.237634829929808E-4 24-25 0.0010690921965220194 9.945043688576924E-5 2.9835131065730774E-4 26-27 0.001690657427058077 9.945043688576924E-5 2.9835131065730774E-4 28-29 0.0034559026817804813 9.945043688576924E-5 2.9835131065730774E-4 30-31 0.006837217535896636 1.4917565532865387E-4 2.9835131065730774E-4 32-33 0.012257266346171058 1.4917565532865387E-4 2.9835131065730774E-4 34-35 0.022227172643969423 1.4917565532865387E-4 2.9835131065730774E-4 36-37 0.034360125944033273 1.4917565532865387E-4 3.2321391987875003E-4 38-39 0.053230846343107985 1.4917565532865387E-4 3.480765291001923E-4 40-41 0.07906309732418654 1.4917565532865387E-4 3.480765291001923E-4 42-43 0.11513874330449933 1.4917565532865387E-4 3.480765291001923E-4 44-45 0.16312357910188302 1.4917565532865387E-4 3.480765291001923E-4 46-47 0.22115290902472934 1.4917565532865387E-4 3.480765291001923E-4 48-49 0.2959147749536064 1.4917565532865387E-4 3.480765291001923E-4 50-51 0.3870362377501924 2.237634829929808E-4 3.480765291001923E-4 52-53 0.49673006963519595 2.486260922144231E-4 3.480765291001923E-4 54-55 0.6218387192374937 2.486260922144231E-4 3.480765291001923E-4 56-57 0.7750172546507996 2.486260922144231E-4 3.480765291001923E-4 58-59 0.9422431642742206 2.486260922144231E-4 3.480765291001923E-4 60-61 1.1337847057162123 2.9835131065730774E-4 3.480765291001923E-4 62-63 1.347826908503609 2.9835131065730774E-4 3.9780174754307695E-4 64-65 1.5854388648329332 3.2321391987875003E-4 4.226643567645193E-4 66-67 1.8461730477381988 3.480765291001923E-4 4.475269659859616E-4 68-69 2.1418889218180333 3.480765291001923E-4 4.475269659859616E-4 70-71 2.4600308694156094 3.480765291001923E-4 4.475269659859616E-4 72-73 2.7884659372308622 3.480765291001923E-4 4.475269659859616E-4 74-75 3.1479046787452534 3.480765291001923E-4 4.475269659859616E-4 76-77 3.5373774521991477 4.475269659859616E-4 4.475269659859616E-4 78-79 3.969763089169251 4.475269659859616E-4 4.723895752074039E-4 80-81 4.419279063892928 4.475269659859616E-4 4.972521844288462E-4 82-83 4.9027573628130945 4.475269659859616E-4 4.972521844288462E-4 84-85 5.416418869328092 4.475269659859616E-4 5.469774028717308E-4 86-87 5.954967847673755 4.475269659859616E-4 5.469774028717308E-4 88-89 6.526260882364056 4.475269659859616E-4 5.469774028717308E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTCGTA 1500 0.0 7.5937824 94-95 CGTATGC 1250 0.0 7.4039383 94-95 TCGTATG 1400 0.0 7.288676 94-95 CTCGTAT 1345 0.0 6.8810687 92-93 ATCTCGT 2030 0.0 6.546365 94-95 TATGCCG 995 2.5465852E-10 6.439451 94-95 AGTCACC 5195 0.0 6.349469 94-95 CAGTCAC 5395 0.0 5.982127 94-95 CAGATCA 3985 0.0 5.7763247 94-95 GTATGCC 1320 3.45608E-11 5.752866 94-95 CCAGTCA 5765 0.0 5.5159345 92-93 TACATTA 1315 6.4090826E-4 5.4516454 1 GTCACCA 5365 0.0 5.396327 94-95 TGATCCT 1255 0.0021031052 5.331463 1 TCCAGTC 6580 0.0 5.265446 94-95 AACTCCA 7715 0.0 5.2597895 94-95 ACCAGAT 4610 0.0 5.1991706 92-93 CTGAACT 8850 0.0 5.175206 92-93 CTCCAGT 6585 0.0 5.1533356 94-95 GAACTCC 8455 0.0 5.1362443 94-95 >>END_MODULE