FastQCFastQC Report
Thu 11 Sep 2014
NYCLib5_CAGATC_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNYCLib5_CAGATC_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2011052
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA15000.07.593782494-95
CGTATGC12500.07.403938394-95
TCGTATG14000.07.28867694-95
CTCGTAT13450.06.881068792-93
ATCTCGT20300.06.54636594-95
TATGCCG9952.5465852E-106.43945194-95
AGTCACC51950.06.34946994-95
CAGTCAC53950.05.98212794-95
CAGATCA39850.05.776324794-95
GTATGCC13203.45608E-115.75286694-95
CCAGTCA57650.05.515934592-93
TACATTA13156.4090826E-45.45164541
GTCACCA53650.05.39632794-95
TGATCCT12550.00210310525.3314631
TCCAGTC65800.05.26544694-95
AACTCCA77150.05.259789594-95
ACCAGAT46100.05.199170692-93
CTGAACT88500.05.17520692-93
CTCCAGT65850.05.153335694-95
GAACTCC84550.05.136244394-95
Produced by FastQC (version 0.11.2)