##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename NYCLib4_GCCAAT_L007_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2408378 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.061995251575958 33.0 22.0 33.0 2.0 33.0 2 25.097475977608166 33.0 15.0 33.0 2.0 33.0 3 25.980231093291835 33.0 22.0 33.0 2.0 33.0 4 28.182698064838657 33.0 22.0 37.0 2.0 37.0 5 28.25755093261938 33.0 22.0 37.0 6.0 37.0 6 29.34513145361733 33.0 27.0 37.0 15.0 37.0 7 29.00642714723353 37.0 27.0 37.0 6.0 37.0 8 28.772142495903882 33.0 27.0 37.0 2.0 37.0 9 29.57434547234695 33.0 27.0 37.0 15.0 37.0 10-11 29.2205033844355 33.0 27.0 37.0 10.5 37.0 12-13 29.534483997113412 33.0 27.0 37.0 15.0 37.0 14-15 29.93511898879661 33.0 27.0 37.0 6.0 40.0 16-17 30.883483406674532 33.0 27.0 37.0 15.0 40.0 18-19 31.22975172501991 33.0 27.0 37.0 15.0 40.0 20-21 31.278902024516086 33.0 27.0 37.0 15.0 40.0 22-23 31.348667028182454 33.0 27.0 37.0 15.0 40.0 24-25 30.96038537139934 33.0 27.0 37.0 15.0 40.0 26-27 30.47697205339029 33.0 27.0 37.0 15.0 40.0 28-29 30.738110877943576 33.0 27.0 37.0 15.0 40.0 30-31 30.697040497795612 33.0 27.0 37.0 15.0 40.0 32-33 30.571612097436535 33.0 27.0 37.0 15.0 40.0 34-35 30.63905894340506 33.0 27.0 37.0 15.0 40.0 36-37 30.885871113255476 33.0 27.0 37.0 15.0 40.0 38-39 30.978166840919492 33.0 27.0 37.0 15.0 40.0 40-41 31.116052588090405 33.0 27.0 37.0 15.0 40.0 42-43 31.083046764253787 33.0 27.0 37.0 15.0 40.0 44-45 30.875072559207897 33.0 27.0 37.0 15.0 40.0 46-47 30.52859123443247 33.0 27.0 37.0 15.0 40.0 48-49 30.425349965827625 33.0 27.0 37.0 15.0 40.0 50-51 29.695015483449858 33.0 27.0 37.0 15.0 38.5 52-53 29.393504466491557 33.0 27.0 37.0 15.0 38.5 54-55 30.391389557619277 33.0 27.0 37.0 15.0 40.0 56-57 30.338980633438773 33.0 27.0 37.0 15.0 40.0 58-59 30.576026271623476 33.0 27.0 37.0 15.0 40.0 60-61 30.34558030342413 33.0 27.0 37.0 15.0 40.0 62-63 30.249404578517158 33.0 27.0 37.0 15.0 40.0 64-65 30.21744676292509 33.0 27.0 37.0 15.0 40.0 66-67 30.092604026444356 33.0 27.0 37.0 15.0 40.0 68-69 29.813802899710925 33.0 27.0 37.0 15.0 40.0 70-71 29.702093483664108 33.0 27.0 37.0 15.0 40.0 72-73 29.436445192573593 33.0 27.0 37.0 15.0 37.0 74-75 29.377188298514604 33.0 27.0 37.0 15.0 37.0 76-77 28.947991760429634 33.0 27.0 37.0 15.0 37.0 78-79 28.32631194106573 33.0 27.0 37.0 15.0 37.0 80-81 28.177440584492963 33.0 24.5 37.0 15.0 37.0 82-83 27.693374960242952 33.0 22.0 35.0 10.5 37.0 84-85 27.75701551002376 33.0 22.0 33.0 15.0 37.0 86-87 27.401420375040793 27.0 22.0 33.0 15.0 37.0 88-89 26.990211877039236 27.0 22.0 33.0 6.0 37.0 90-91 26.81872509215746 27.0 22.0 33.0 6.0 37.0 92-93 26.653596113234716 27.0 22.0 33.0 6.0 37.0 94-95 26.380934803423713 27.0 22.0 33.0 6.0 37.0 96-97 26.19106053950003 27.0 22.0 33.0 6.0 37.0 98-99 25.91056428849624 27.0 22.0 33.0 6.0 37.0 100-101 25.371102252221206 27.0 18.5 33.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 8.411117082392106 2306 2 8.615586866276686 2306 3 7.556056574755459 2306 4 9.198013464730824 2306 5 9.003190105303648 2306 6 8.038206546342188 2306 7 8.269448654618891 2306 8 8.711204986686031 2306 9 7.577777244300503 2306 10-11 7.919232003805327 2306 12-13 7.507032061024255 2306 14-15 8.20930142885845 2306 16-17 6.861719744695421 2306 18-19 6.38549723993566 2306 20-21 6.236610003745906 2306 22-23 5.8543050725534975 2306 24-25 6.088077244071144 2306 26-27 6.698318370507735 2306 28-29 5.7281634726637485 2306 30-31 5.7249684588846605 2306 32-33 5.602260930706091 2306 34-35 5.060145845246847 2306 36-37 5.189673015699739 2306 38-39 4.98598616913312 2306 40-41 4.09125631407473 2306 42-43 3.9234535978690843 2306 44-45 3.717220942347925 2306 46-47 3.8889285740765374 2306 48-49 3.8577623708161646 2306 50-51 3.7307897646508508 2306 52-53 4.271434811793483 2306 54-55 3.855015496964292 2306 56-57 4.090951727867552 2306 58-59 3.8591260582239 2306 60-61 3.7258690224437565 2306 62-63 3.2352028310271272 2306 64-65 2.6811720686553038 2306 66-67 2.6009007495619834 2306 68-69 2.951480773028546 2306 70-71 2.839901366730551 2306 72-73 2.2037375792933567 2306 74-75 1.9339477384077028 2306 76-77 2.252831717873182 2306 78-79 2.44143479679299 2306 80-81 2.1627165619370246 2306 82-83 2.0652955478384705 2306 84-85 1.7083320396495871 2306 86-87 1.5666486734808025 2306 88-89 1.5590537591056304 2306 90-91 1.4226152948829238 2306 92-93 1.5137218668750272 2306 94-95 1.6181005109006321 2306 96-97 1.4010145830176413 2306 98-99 1.3123609886669065 2306 100-101 1.0647270228673023 2307 1 8.376311628742883 2307 2 8.570912685569755 2307 3 7.640025455343505 2307 4 9.285161901878784 2307 5 9.088828061191823 2307 6 7.585454319190006 2307 7 8.14320096288202 2307 8 8.589701483105774 2307 9 7.713882244935181 2307 10-11 7.936339538736874 2307 12-13 7.4498564654583745 2307 14-15 8.434673832716395 2307 16-17 7.052060947572215 2307 18-19 6.6387187840099955 2307 20-21 6.576173706309181 2307 22-23 6.162565374215934 2307 24-25 6.404529139859925 2307 26-27 7.105259546286447 2307 28-29 6.27863410706588 2307 30-31 6.141758675896902 2307 32-33 6.300195687264047 2307 34-35 5.982850000800216 2307 36-37 5.817907944607001 2307 38-39 5.5427142415690085 2307 40-41 5.002520752700715 2307 42-43 4.745108699480014 2307 44-45 4.751359980249948 2307 46-47 5.0741592911721085 2307 48-49 4.8837072751014325 2307 50-51 4.671514526049499 2307 52-53 5.191811476649029 2307 54-55 4.894836940619911 2307 56-57 5.167606513283285 2307 58-59 4.651740559455 2307 60-61 4.671925396228641 2307 62-63 4.275091733476934 2307 64-65 4.191793764167276 2307 66-67 3.718427773940636 2307 68-69 3.9803090872850255 2307 70-71 3.603622061584005 2307 72-73 3.087496358101678 2307 74-75 2.7528762483538785 2307 76-77 2.998869406354924 2307 78-79 3.322027499343889 2307 80-81 3.2193418518399035 2307 82-83 3.2714682776371475 2307 84-85 2.5295525638034633 2307 86-87 2.6519600279892366 2307 88-89 2.7871669414056015 2307 90-91 2.5492290933052786 2307 92-93 2.4187505390583617 2307 94-95 2.669167104511441 2307 96-97 2.3880475630932203 2307 98-99 2.0768312532581312 2307 100-101 1.914000457475769 1101 1 8.104593289891163 1101 2 7.9421136013094085 1101 3 7.438050695209618 1101 4 8.93504545393635 1101 5 8.633047762879794 1101 6 7.456643560081353 1101 7 7.315115493128509 1101 8 8.164644145344166 1101 9 7.460632128995851 1101 10-11 7.523608605998952 1101 12-13 7.263397251213426 1101 14-15 7.787959751523875 1101 16-17 6.927404642776523 1101 18-19 6.067414108537633 1101 20-21 5.991618212865358 1101 22-23 5.435055177796432 1101 24-25 6.135683353123881 1101 26-27 6.759137225779732 1101 28-29 6.0881914384313625 1101 30-31 6.012294895900055 1101 32-33 5.937984173536353 1101 34-35 5.708384084652909 1101 36-37 5.577185422537681 1101 38-39 5.316900076949487 1101 40-41 4.856137613000325 1101 42-43 4.681681868721242 1101 44-45 4.637540651109081 1101 46-47 4.946768182058435 1101 48-49 4.728355597845717 1101 50-51 4.275685671927768 1101 52-53 5.194402175578659 1101 54-55 4.88444372479335 1101 56-57 4.944757066578621 1101 58-59 4.553635364216852 1101 60-61 4.8746734705722226 1101 62-63 4.392908022071175 1101 64-65 4.121370212229916 1101 66-67 3.6556886996335756 1101 68-69 3.8747560709126496 1101 70-71 3.8767592605482406 1101 72-73 3.4173300882257323 1101 74-75 2.9424050229633387 1101 76-77 3.078675143814632 1101 78-79 3.6107797115927838 1101 80-81 3.150975327259605 1101 82-83 3.51525824087409 1101 84-85 2.7067244747503416 1101 86-87 2.804223534478677 1101 88-89 2.9037989457173587 1101 90-91 2.7043394325663535 1101 92-93 2.358959094233075 1101 94-95 2.5595567701052566 1101 96-97 2.506336548185942 1101 98-99 2.340022733716747 1101 100-101 1.9599089086325954 2201 1 7.219162517853171 2201 2 7.912065358938712 2201 3 6.378107754652142 2201 4 8.462021097690066 2201 5 3.9889287680808643 2201 6 5.682994440210791 2201 7 6.676263875575401 2201 8 7.833462831499855 2201 9 6.639084197829373 2201 10-11 4.341713340281764 2201 12-13 6.544971103107663 2201 14-15 7.171564685425569 2201 16-17 6.037054111579955 2201 18-19 2.196046234508348 2201 20-21 5.210164006532157 2201 22-23 5.331275830049382 2201 24-25 5.535326794489208 2201 26-27 6.291626054668626 2201 28-29 5.038127402617956 2201 30-31 5.292753237293603 2201 32-33 5.489728963666725 2201 34-35 5.05393892908052 2201 36-37 5.175027080641087 2201 38-39 4.541758388708885 2201 40-41 4.295158906594633 2201 42-43 4.158358987368018 2201 44-45 4.055374562262447 2201 46-47 4.478585326598505 2201 48-49 4.282334038550228 2201 50-51 4.010442267239256 2201 52-53 4.803240306853333 2201 54-55 4.411421769461327 2201 56-57 4.424673247231642 2201 58-59 4.036433327967167 2201 60-61 4.095725647241093 2201 62-63 3.9014077158974416 2201 64-65 3.7147903008853937 2201 66-67 3.3887065185333007 2201 68-69 3.531549377803916 2201 70-71 3.339556522316947 2201 72-73 2.77325150881698 2201 74-75 2.4406121116512836 2201 76-77 2.7629554320341008 2201 78-79 3.279752170590882 2201 80-81 2.7703729653087485 2201 82-83 2.9979574241516573 2201 84-85 2.5266699099720746 2201 86-87 2.386209211767447 2201 88-89 2.3927770914523983 2201 90-91 2.2648475026108557 2201 92-93 2.206649323243713 2201 94-95 2.4516332156728673 2201 96-97 2.273219605825716 2201 98-99 2.211198934756961 2201 100-101 1.8339109857664262 2304 1 1.9359173728752452 2304 2 5.130604629399876 2304 3 5.884209669485102 2304 4 4.497824637480065 2304 5 6.753442546512286 2304 6 6.420114544225093 2304 7 7.292421394365384 2304 8 6.731809766088606 2304 9 5.942935044943805 2304 10-11 6.844503862298211 2304 12-13 6.738017677749376 2304 14-15 7.139989390953719 2304 16-17 5.695817463057182 2304 18-19 5.484192693785843 2304 20-21 5.27214063795012 2304 22-23 5.083390951821254 2304 24-25 5.229931652042367 2304 26-27 6.004973269008957 2304 28-29 5.223300635453864 2304 30-31 5.202214968609525 2304 32-33 5.332672096831143 2304 34-35 5.029295812168346 2304 36-37 5.224867155987511 2304 38-39 4.827480667325474 2304 40-41 4.243837480021437 2304 42-43 3.7017994122707023 2304 44-45 3.605353181248791 2304 46-47 4.685339728689449 2304 48-49 4.279618783030454 2304 50-51 3.9106395587110967 2304 52-53 4.5200457835899925 2304 54-55 4.258856580682298 2304 56-57 5.069636852883161 2304 58-59 4.204249307377445 2304 60-61 4.300513795365564 2304 62-63 4.008832988921661 2304 64-65 3.7675151478879236 2304 66-67 3.5750786867146225 2304 68-69 3.8099768009188573 2304 70-71 3.871171781300305 2304 72-73 3.4155468172497585 2304 74-75 2.8261550828262116 2304 76-77 3.181512087057772 2304 78-79 3.429578458180842 2304 80-81 3.0064057015722803 2304 82-83 3.3480408833732014 2304 84-85 2.837309728479905 2304 86-87 2.63317623838115 2304 88-89 2.7468450124718657 2304 90-91 2.643745041596233 2304 92-93 2.4388418029883674 2304 94-95 2.6892738574496384 2304 96-97 2.648019117192959 2304 98-99 2.464507996310303 2304 100-101 2.1982894177188506 1102 1 7.051066564667742 1102 2 7.543088950955923 1102 3 6.189495863894084 1102 4 6.36197133933096 1102 5 6.507175784069087 1102 6 6.00724016903667 1102 7 6.783452021763672 1102 8 6.438984817543673 1102 9 4.994533780991386 1102 10-11 6.079937145634219 1102 12-13 5.851005570454852 1102 14-15 6.142329712747969 1102 16-17 5.244393469548143 1102 18-19 5.284753023693803 1102 20-21 5.460781814661541 1102 22-23 5.253975027360649 1102 24-25 2.0252271883847257 1102 26-27 5.553255804804195 1102 28-29 4.915495840920567 1102 30-31 4.999663988078709 1102 32-33 4.971157326527322 1102 34-35 4.9305621838135245 1102 36-37 4.921708019316142 1102 38-39 3.9266959077034684 1102 40-41 3.9095961306145135 1102 42-43 4.252016584307434 1102 44-45 -4.015152548594411 1102 46-47 1.5133154694377708 1102 48-49 -0.6829744036853853 1102 50-51 2.9623423214669344 1102 52-53 -0.06637640480442286 1102 54-55 2.7864627381701723 1102 56-57 -0.9180937644660396 1102 58-59 2.9245748979476787 1102 60-61 3.9751936201391764 1102 62-63 3.5827587596311616 1102 64-65 3.3064730570813516 1102 66-67 3.1734966991531586 1102 68-69 3.52319861414718 1102 70-71 3.3669796352237285 1102 72-73 2.861832561310557 1102 74-75 2.680039801135287 1102 76-77 3.022173689478489 1102 78-79 -1.1407125188514016 1102 80-81 1.744154928350504 1102 82-83 2.657793340748306 1102 84-85 2.3626954088964602 1102 86-87 2.4504822629976992 1102 88-89 2.431616172323139 1102 90-91 2.202359346292141 1102 92-93 2.1438229888556215 1102 94-95 2.4735267447025535 1102 96-97 2.466990127424424 1102 98-99 2.3681889413715425 1102 100-101 2.058619139582323 2202 1 -20.877832017665956 2202 2 -7.930336778738711 2202 3 -0.64312909352563 2202 4 1.8032080880032524 2202 5 1.3546390278523681 2202 6 1.6999531104713483 2202 7 4.059330490199393 2202 8 2.6081131390916994 2202 9 3.215361084499083 2202 10-11 2.264078618489144 2202 12-13 1.5277522194721342 2202 14-15 2.813410126045362 2202 16-17 1.7445988265481205 2202 18-19 1.7098955390512813 2202 20-21 1.1472887223840615 2202 22-23 1.4590172761483906 2202 24-25 2.3088352931562284 2202 26-27 3.5584951231538398 2202 28-29 -0.9515800537226617 2202 30-31 1.0340937780464543 2202 32-33 1.5758163241702583 2202 34-35 1.5755185347345169 2202 36-37 2.9188559117920647 2202 38-39 2.396647086120012 2202 40-41 1.809852984040031 2202 42-43 0.8375527186805272 2202 44-45 0.7170170855466331 2202 46-47 -1.4266611028321172 2202 48-49 0.9355868284794759 2202 50-51 1.8015332731848908 2202 52-53 1.6259628874826575 2202 54-55 -0.9897551437206893 2202 56-57 1.0801418399682525 2202 58-59 1.8387000073990194 2202 60-61 1.9756853509906271 2202 62-63 -2.045327710926145 2202 64-65 -0.15136888832900652 2202 66-67 0.49354581222582183 2202 68-69 1.3346948910870715 2202 70-71 -2.9073939487674565 2202 72-73 -4.661021906704502 2202 74-75 -1.2023036405863081 2202 76-77 -0.18736406580318388 2202 78-79 1.553396913053291 2202 80-81 1.5679901717954259 2202 82-83 -0.755186616225604 2202 84-85 -0.26449627554532995 2202 86-87 0.7204563604671179 2202 88-89 1.118566200786205 2202 90-91 1.226651370461738 2202 92-93 1.204174227627572 2202 94-95 1.551728524958559 2202 96-97 1.070193336549064 2202 98-99 1.0422026219313878 2202 100-101 0.8406243680984282 2305 1 5.678631578173569 2305 2 7.244282249299953 2305 3 6.769723180936165 2305 4 7.7335658371847735 2305 5 7.943876959327252 2305 6 6.162510466532169 2305 7 3.2115304241601024 2305 8 6.148119088858227 2305 9 6.512585409387487 2305 10-11 4.961279307852145 2305 12-13 5.441783933908262 2305 14-15 5.745340396962224 2305 16-17 5.21166912724496 2305 18-19 4.4117489614130605 2305 20-21 5.259042314828168 2305 22-23 5.140058002456019 2305 24-25 5.417988783114829 2305 26-27 6.134034184461356 2305 28-29 5.271487907563358 2305 30-31 5.376771515639945 2305 32-33 5.382969321949222 2305 34-35 5.346097990595663 2305 36-37 4.7843481615202705 2305 38-39 4.602855947845562 2305 40-41 4.211812668920455 2305 42-43 3.993426867100723 2305 44-45 3.131813352769033 2305 46-47 3.7537992486713634 2305 48-49 3.3851469818650806 2305 50-51 2.413798903782265 2305 52-53 3.9283404967356326 2305 54-55 4.244271407501813 2305 56-57 4.650385102870057 2305 58-59 4.324860570103631 2305 60-61 4.396006178911538 2305 62-63 3.846615884664182 2305 64-65 3.9588846075482103 2305 66-67 3.6435607255804037 2305 68-69 3.9741581193493936 2305 70-71 3.641565075720038 2305 72-73 -0.9867389158721274 2305 74-75 1.9362046267856101 2305 76-77 2.9393628461433856 2305 78-79 3.592658947312728 2305 80-81 3.277782521828602 2305 82-83 3.4653394552775083 2305 84-85 2.9115169369859366 2305 86-87 2.753912894463337 2305 88-89 2.858313366859452 2305 90-91 2.7650997897240295 2305 92-93 2.7840782681732072 2305 94-95 2.922881619304416 2305 96-97 2.7639709632463827 2305 98-99 2.6853980826644843 2305 100-101 2.1828959796129404 1103 1 -20.877832017665956 1103 2 -7.994469870565224 1103 3 -0.7111844244046779 1103 4 3.1554569364340317 1103 5 4.625895059395315 1103 6 4.650592703210457 1103 7 4.216828108628423 1103 8 4.378615074577681 1103 9 4.319898978030199 1103 10-11 4.9979626747906245 1103 12-13 4.848200355147334 1103 14-15 4.221611901071466 1103 16-17 2.9449925006380937 1103 18-19 3.169500439286743 1103 20-21 2.5249135567362018 1103 22-23 2.686554323261827 1103 24-25 3.0875541591199465 1103 26-27 4.282792743543688 1103 28-29 3.7726348448624485 1103 30-31 3.605679798102301 1103 32-33 3.117254331428537 1103 34-35 2.551288296871544 1103 36-37 2.7128211212477567 1103 38-39 2.53493813124976 1103 40-41 2.752961216023831 1103 42-43 2.4987076621355797 1103 44-45 2.8137091733387756 1103 46-47 2.1259927556396576 1103 48-49 -1.0137241090996731 1103 50-51 -1.8859263871695902 1103 52-53 1.8492185195099253 1103 54-55 2.445926610586156 1103 56-57 3.4046489609880055 1103 58-59 2.7841448750522986 1103 60-61 3.2691515743501185 1103 62-63 2.9549421051332416 1103 64-65 2.7484830907033633 1103 66-67 2.6247921254886 1103 68-69 2.8933646672765896 1103 70-71 2.941393247449483 1103 72-73 2.3177363593484266 1103 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56-57 -26.525073044727765 1209 58-59 -26.827442550252975 1209 60-61 -26.56820877898841 1209 62-63 -26.75628158933285 1209 64-65 -26.650451697553326 1209 66-67 -14.395246990010376 1209 68-69 -26.322275758808107 1209 70-71 -26.16551763849492 1209 72-73 -16.59851317155426 1209 74-75 -17.36155416469532 1209 76-77 -14.115037021778026 1209 78-79 -15.147223282614076 1209 80-81 -25.084525551475014 1209 82-83 -16.916944128454816 1209 84-85 -13.93943512949362 1209 86-87 -14.138313730378446 1209 88-89 -11.17800962031792 1209 90-91 -13.918247301795425 1209 92-93 -18.16112975560094 1209 94-95 -23.440540867074983 1209 96-97 -19.183326928999506 1209 98-99 -10.500285148858271 1209 100-101 -5.785284087527007 1212 1 -20.877832017665956 1212 2 -20.713617701170577 1212 3 -21.61115844363045 1212 4 -23.65722168138878 1212 5 -23.85552868703233 1212 6 -24.93605317883683 1212 7 -24.51011395424505 1212 8 -24.15970656740725 1212 9 -25.07656763667492 1212 10-11 -24.820171905619873 1212 12-13 -25.141797046775245 1212 14-15 -25.51481314439489 1212 16-17 -26.624635974290456 1212 18-19 -21.294806206353623 1212 20-21 -27.06549953757401 1212 22-23 -27.320070774166073 1212 24-25 -23.410334298770053 1212 26-27 -26.08327636531576 1212 28-29 -20.966142971181586 1212 30-31 -15.751292112451328 1212 32-33 -26.348580950462864 1212 34-35 -22.549057912050134 1212 36-37 -26.809262536845253 1212 38-39 -27.125103437989004 1212 40-41 -21.663322385274 1212 42-43 -17.95806304846881 1212 44-45 -14.72229413769943 1212 46-47 -18.14772723807662 1212 48-49 -21.288870779633243 1212 50-51 -13.527685194954156 1212 52-53 -6.169759529756874 1212 54-55 -4.828722648961779 1212 56-57 -4.460538161006834 1212 58-59 -3.197209992113443 1212 60-61 -14.954836685965153 1212 62-63 -13.128374612588665 1212 64-65 -17.388242395227742 1212 66-67 -13.690186697159387 1212 68-69 -8.960066456482526 1212 70-71 -16.411447871053056 1212 72-73 -12.83031765045176 1212 74-75 -9.224151063920129 1212 76-77 -20.252375523070015 1212 78-79 -10.77340760647282 1212 80-81 -8.608944156126178 1212 82-83 -5.796552224923374 1212 84-85 -4.993720228546163 1212 86-87 -3.9479605865369294 1212 88-89 -3.8100940303093083 1212 90-91 -4.015706388789397 1212 92-93 -3.7882615385466742 1212 94-95 -3.468447843819167 1212 96-97 -13.500811855786756 1212 98-99 -10.971839843087384 1212 100-101 -7.145124742135106 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 390.0 3 863.0 4 336.0 5 2271.0 6 3125.0 7 2220.0 8 4494.0 9 9059.0 10 11187.0 11 12755.0 12 17591.0 13 23844.0 14 27778.0 15 29637.0 16 31148.0 17 34454.0 18 40200.0 19 45974.0 20 50519.0 21 57811.0 22 66855.0 23 74245.0 24 79994.0 25 84431.0 26 90485.0 27 96051.0 28 99481.0 29 100950.0 30 104569.0 31 111231.0 32 123095.0 33 145835.0 34 170416.0 35 189738.0 36 203142.0 37 191643.0 38 70337.0 39 224.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 28.562892878223327 23.76855892243532 25.3269336105237 22.34161458881765 2 27.07810582139477 35.03316873893204 19.148996973586815 18.73972846608637 3 16.79218813452948 29.610006645254185 29.911442758518845 23.686362461697495 4 20.219081232091423 29.492636614521533 29.46019131409126 20.828090839295786 5 20.43021443370387 28.338814598829092 29.462023489566963 21.76894747790007 6 20.57757084504288 30.371578047649706 27.460872354777827 21.589978752529593 7 20.777870734287763 30.117678637148927 29.052870526749796 20.051580101813514 8 20.084312875089427 29.700549358895945 29.370544799439518 20.844592966575107 9 19.929693961952026 28.519921510655898 30.582422610562627 20.967961916829452 10-11 21.118495919738297 28.786561302590464 28.755717399627873 21.339225378043363 12-13 20.967507267029774 29.384470600232703 28.43104244789343 21.216979684844095 14-15 21.138400562953972 29.325086378081632 28.501296766181618 21.03521629278278 16-17 21.464981152828614 29.388649681724477 28.03256282248134 21.113806342965574 18-19 21.335829767815294 29.567951854355307 28.162924731256584 20.933293646572814 20-21 21.5438712210511 29.44350224394775 28.05467560057151 20.957950934429636 22-23 21.13219011916402 29.33401477325709 28.465822547980256 21.067972559598637 24-25 21.131640071124423 29.304959967738085 28.614137939502875 20.949262021634613 26-27 21.15139290476742 29.443200961623496 28.394836662417266 21.010569471191822 28-29 21.154444205549012 29.41510998809117 28.621808024053596 20.80863778230622 30-31 21.346862174575612 29.125379117153845 28.448823700693517 21.07893500757703 32-33 21.137656222941217 29.561114054535075 28.348880613009474 20.95234910951423 34-35 21.23888272115972 29.20538453353952 28.600523176037164 20.9552095692636 36-37 21.234698075833354 29.305512348202832 28.38400403413146 21.075785541832353 38-39 21.012750949481656 29.291927726296553 28.837600252900252 20.857721071321535 40-41 21.35148393684195 29.05967786495455 28.43131178693245 21.15752641127105 42-43 21.066345471479835 29.690699307313267 28.397922085948107 20.84503313525879 44-45 21.092040727437997 29.093795567450876 28.782111573317177 21.032052131793954 46-47 21.20980108007124 29.302165154120512 28.4522713039904 21.035762461817846 48-49 20.982268499568164 29.19057040414056 28.84055952940365 20.986601566887625 50-51 21.36150616052056 29.113013723916282 28.443747343239252 21.08173277232391 52-53 21.078965088910046 29.38738313068423 28.59747873936542 20.936173041040306 54-55 21.0914128255856 29.13399767365958 28.840888933637952 20.933700567116865 56-57 21.228053064261594 29.31761735939225 28.53320091880562 20.92112865754053 58-59 21.157489851207306 29.326023108020287 28.672829086735252 20.843657954037152 60-61 21.39353291990711 29.011578306937697 28.528852263128446 21.06603651002675 62-63 21.331432645524718 29.33136746953597 28.555167400975844 20.78203248396347 64-65 21.543390103387278 29.08308666244403 28.58440350226399 20.789119731904705 66-67 21.695221134413085 29.216146761095978 28.249355869512467 20.839276234978467 68-69 21.495888153763456 29.238567459898825 28.582962002890266 20.682582383447453 70-71 21.854224487124846 29.0596254073301 28.293752537947558 20.792397567597497 72-73 21.717945553246388 29.411773261672963 28.295518816664732 20.574762368415914 74-75 21.904829398441894 29.09395731854062 28.334169483043343 20.667043799974145 76-77 22.080075623640564 29.221666373260735 28.032600597213168 20.66565740588553 78-79 22.01618944355439 29.27508647329718 28.272850903947607 20.435873179200826 80-81 22.434019152294535 29.062616922253376 27.97851535050598 20.524848574946112 82-83 22.23365734549904 29.319069367878864 28.091276633534957 20.35599665308714 84-85 22.584974318447923 28.988763438045595 27.937984515449234 20.488277728057252 86-87 22.67197113575253 29.145001405847786 27.752744520362764 20.43028293803692 88-89 22.708275372433626 29.155799106188386 27.89229131634735 20.243634205030638 90-91 23.094537155091736 29.021955772616124 27.4895773247802 20.39392974751194 92-93 23.198295573294402 29.145426319833717 27.448673277662884 20.207604829209 94-95 23.469163868438066 28.92956806473815 27.430204183786266 20.171063883037522 96-97 23.7429030226674 29.122769216278655 27.03104936699734 20.103278394056606 98-99 23.791133455210236 29.12672428120326 27.134099218879843 19.948043044706665 100-101 24.413263071194624 28.86027128044445 26.66889747500381 20.057568173357115 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 184.0 1 188.5 2 193.5 3 196.0 4 216.5 5 253.5 6 271.0 7 308.5 8 406.5 9 532.0 10 738.5 11 1017.5 12 1400.0 13 1843.0 14 2388.0 15 3115.5 16 3929.5 17 4939.5 18 6267.0 19 7962.0 20 10126.0 21 12792.0 22 16018.5 23 19884.0 24 24331.5 25 29159.5 26 34352.0 27 39932.0 28 45598.0 29 51022.5 30 56495.0 31 62003.0 32 67411.5 33 72223.0 34 76598.5 35 81397.5 36 85778.0 37 88877.5 38 90528.5 39 90488.0 40 89011.0 41 86387.5 42 82723.0 43 78266.0 44 73502.5 45 69405.0 46 65730.5 47 62287.5 48 59212.0 49 55614.5 50 51801.5 51 48367.0 52 45084.5 53 42096.5 54 39594.5 55 37175.0 56 35012.5 57 32960.0 58 31020.5 59 29141.5 60 26503.5 61 23683.5 62 21254.5 63 19152.5 64 16922.5 65 14776.5 66 12907.0 67 11142.5 68 9601.0 69 8107.0 70 6910.5 71 5837.5 72 4769.5 73 4012.0 74 3346.5 75 2671.0 76 2154.5 77 1792.5 78 1459.5 79 1153.0 80 915.0 81 762.5 82 629.0 83 491.0 84 378.0 85 299.0 86 248.0 87 213.0 88 170.0 89 122.5 90 97.0 91 73.0 92 44.0 93 32.0 94 25.5 95 20.0 96 14.0 97 10.0 98 6.0 99 2.5 100 1.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 14.037663522918745 2 11.370225105859628 3 10.024090902673915 4 11.953522246092598 5 9.375272486295756 6 5.514624365444295 7 9.969365274055816 8 10.389814223514747 9 5.624449318171815 10-11 6.574487061416439 12-13 5.538042616233831 14-15 7.567894242515087 16-17 2.756294900551325 18-19 3.1882868885199915 20-21 3.1831174342233655 22-23 3.2394624099705274 24-25 3.627960394921395 26-27 4.454014278489506 28-29 1.7989908560865446 30-31 2.319943131850565 32-33 3.134640824654602 34-35 2.048058901052908 36-37 3.1671730932602773 38-39 1.4250047127153629 40-41 1.1823517736833669 42-43 0.5635535617747712 44-45 1.332556600334333 46-47 1.4077939592539046 48-49 1.3000035708680282 50-51 1.5880189903744346 52-53 2.5435583616857484 54-55 1.8510175728228706 56-57 1.872318215828246 58-59 1.604316265968216 60-61 1.418112106986528 62-63 0.6169504953126128 64-65 1.2753811901620096 66-67 0.5840030094943568 68-69 0.8167322571456807 70-71 1.0198565175400207 72-73 0.08416452899005056 74-75 0.10417799863642668 76-77 0.46687023382542103 78-79 0.8932983111455095 80-81 0.28826454983395466 82-83 0.5288829245243064 84-85 0.06610258024280241 86-87 0.09784593614457532 88-89 0.08065594354374604 90-91 0.08661431054427503 92-93 0.168183731955698 94-95 0.45790154203368405 96-97 0.371245709768151 98-99 0.06552127614518984 100-101 0.2350752249024032 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2408378.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.43556417120833 #Duplication Level Percentage of deduplicated Percentage of total 1 98.939025903955 97.39118833405604 2 0.7758373769538242 1.5273997981112017 3 0.15712421071867747 0.46399830981146495 4 0.08294636757564987 0.3265948995304606 5 0.025564363594936784 0.12582212765727507 6 0.006401703690112344 0.03780931886358689 7 0.005496281303576068 0.03787206856728222 8 0.0016057410976884272 0.012644962469108453 9 0.0 0.0 >10 0.0059980511105242355 0.07667018093358052 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.2456516377412516E-4 0.0 0.0 2 1.2456516377412516E-4 0.0 4.152172125804172E-5 3 1.6608688503216688E-4 0.0 4.152172125804172E-5 4 2.076086062902086E-4 0.0 4.152172125804172E-5 5 2.076086062902086E-4 0.0 4.152172125804172E-5 6 2.076086062902086E-4 0.0 8.304344251608344E-5 7 2.9065204880629204E-4 0.0 8.304344251608344E-5 8 3.736954913223755E-4 0.0 8.304344251608344E-5 9 4.152172125804172E-4 0.0 8.304344251608344E-5 10-11 4.152172125804172E-4 0.0 1.038043031451043E-4 12-13 5.397823763545423E-4 0.0 1.2456516377412516E-4 14-15 6.228258188706257E-4 0.0 1.2456516377412516E-4 16-17 6.228258188706257E-4 0.0 1.2456516377412516E-4 18-19 6.643475401286675E-4 0.0 1.4532602440314602E-4 20-21 7.2663012201573E-4 0.0 1.6608688503216688E-4 22-23 8.304344251608344E-4 0.0 1.6608688503216688E-4 24-25 9.549995889349595E-4 2.076086062902086E-5 2.076086062902086E-4 26-27 0.0012456516377412517 4.152172125804172E-5 2.076086062902086E-4 28-29 0.0019930426203860025 4.152172125804172E-5 2.076086062902086E-4 30-31 0.0032802159793852955 4.152172125804172E-5 2.076086062902086E-4 32-33 0.006249019049335279 4.152172125804172E-5 2.491303275482503E-4 34-35 0.010899451830235952 4.152172125804172E-5 2.9065204880629204E-4 36-37 0.01868477456611877 4.152172125804172E-5 3.114129094353129E-4 38-39 0.030767595452208914 4.152172125804172E-5 3.3217377006433376E-4 40-41 0.049576935182101815 4.152172125804172E-5 3.529346306933546E-4 42-43 0.0755487718290069 4.152172125804172E-5 3.736954913223755E-4 44-45 0.1138525596895504 4.152172125804172E-5 3.736954913223755E-4 46-47 0.16585851556524764 4.152172125804172E-5 4.152172125804172E-4 48-49 0.23094381363722805 4.152172125804172E-5 4.567389338384589E-4 50-51 0.31041638812511985 4.152172125804172E-5 4.567389338384589E-4 52-53 0.4098609105381298 4.152172125804172E-5 4.567389338384589E-4 54-55 0.531415749521047 8.304344251608344E-5 4.567389338384589E-4 56-57 0.6773853606036926 8.304344251608344E-5 4.567389338384589E-4 58-59 0.8491191997269532 8.304344251608344E-5 4.567389338384589E-4 60-61 1.0433993334933302 1.2456516377412516E-4 4.774997944674798E-4 62-63 1.2626132608751617 1.2456516377412516E-4 4.982606550965006E-4 64-65 1.5092107634266714 1.2456516377412516E-4 5.190215157255215E-4 66-67 1.7842091233186816 1.6608688503216688E-4 5.397823763545423E-4 68-69 2.0843073637111784 2.076086062902086E-4 5.397823763545423E-4 70-71 2.413802982754368 2.076086062902086E-4 5.605432369835631E-4 72-73 2.7594297905063074 2.076086062902086E-4 6.020649582416049E-4 74-75 3.124883220158962 2.076086062902086E-4 6.228258188706257E-4 76-77 3.510080228269815 2.076086062902086E-4 6.228258188706257E-4 78-79 3.9316087424814548 2.076086062902086E-4 6.228258188706257E-4 80-81 4.390423762382815 2.076086062902086E-4 6.228258188706257E-4 82-83 4.872864641680002 2.076086062902086E-4 6.228258188706257E-4 84-85 5.383332682826367 2.076086062902086E-4 6.228258188706257E-4 86-87 5.929550925975906 2.076086062902086E-4 6.228258188706257E-4 88-89 6.5078862205185395 2.076086062902086E-4 6.228258188706257E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTGAG 1070 5.9911017E-6 7.644315 2 CGTGAGC 1015 1.9933321E-4 6.984067 3 GGCGTGA 1350 1.2512031E-5 6.7165523 1 TATCCCT 1100 3.329066E-4 6.6974716 1 GTCAGGC 1200 0.0015184883 5.9073563 2 TACTCTT 1360 0.0042496035 5.417073 1 GTCGTGT 9230 0.0 5.374091 94-95 TACTGAG 1430 0.00765669 5.1519012 1 TCGTGTA 7875 0.0 5.1195526 94-95 >>END_MODULE