FastQCFastQC Report
Thu 11 Sep 2014
NYCLib4_GCCAAT_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNYCLib4_GCCAAT_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2408378
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGAG10705.9911017E-67.6443152
CGTGAGC10151.9933321E-46.9840673
GGCGTGA13501.2512031E-56.71655231
TATCCCT11003.329066E-46.69747161
GTCAGGC12000.00151848835.90735632
TACTCTT13600.00424960355.4170731
GTCGTGT92300.05.37409194-95
TACTGAG14300.007656695.15190121
TCGTGTA78750.05.119552694-95
Produced by FastQC (version 0.11.2)