##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename NYCLib4_GCCAAT_L007_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2408378 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.251906469831564 33.0 33.0 33.0 27.0 33.0 2 31.64384286851981 33.0 33.0 33.0 27.0 33.0 3 31.79166061141565 33.0 33.0 33.0 27.0 33.0 4 35.633354066512815 37.0 37.0 37.0 33.0 37.0 5 35.156029078491834 37.0 37.0 37.0 33.0 37.0 6 34.95153169477549 37.0 33.0 37.0 33.0 37.0 7 34.76098145722972 37.0 33.0 37.0 27.0 37.0 8 34.80275646098744 37.0 33.0 37.0 27.0 37.0 9 34.71649591550828 37.0 33.0 37.0 27.0 37.0 10-11 34.57021945890554 37.0 33.0 37.0 27.0 37.0 12-13 34.564143793042454 37.0 33.0 37.0 27.0 37.0 14-15 35.50087901483903 37.0 33.0 40.0 27.0 40.0 16-17 35.41827881669738 37.0 33.0 40.0 27.0 40.0 18-19 35.64307139493883 37.0 33.0 40.0 27.0 40.0 20-21 35.35111161952152 37.0 33.0 40.0 27.0 40.0 22-23 35.38999671147968 37.0 33.0 40.0 27.0 40.0 24-25 35.25096143545573 37.0 33.0 40.0 27.0 40.0 26-27 34.9013479196372 37.0 33.0 40.0 27.0 40.0 28-29 34.56877284213691 37.0 33.0 40.0 27.0 40.0 30-31 34.48937064696655 37.0 33.0 40.0 27.0 40.0 32-33 34.51642620053829 37.0 33.0 40.0 27.0 40.0 34-35 34.40733576705982 37.0 33.0 40.0 27.0 40.0 36-37 34.151628814081505 37.0 33.0 40.0 27.0 40.0 38-39 34.118538493542125 37.0 33.0 37.0 24.5 40.0 40-41 33.90195330633314 37.0 33.0 37.0 22.0 40.0 42-43 33.5665281778857 37.0 33.0 37.0 22.0 40.0 44-45 33.51554137265828 37.0 33.0 37.0 22.0 40.0 46-47 33.41774526257922 37.0 33.0 37.0 22.0 40.0 48-49 33.463523998309235 37.0 33.0 37.0 22.0 40.0 50-51 33.74190720891821 37.0 33.0 37.0 22.0 40.0 52-53 33.613973180289804 37.0 33.0 37.0 22.0 40.0 54-55 33.29008922187464 37.0 33.0 37.0 22.0 40.0 56-57 33.354312944230514 37.0 33.0 37.0 22.0 40.0 58-59 33.078017653375014 37.0 33.0 37.0 22.0 40.0 60-61 33.003036691084205 37.0 33.0 37.0 22.0 40.0 62-63 32.99460466753973 37.0 33.0 37.0 22.0 40.0 64-65 32.662725286479116 37.0 33.0 37.0 22.0 40.0 66-67 32.31794199249453 37.0 30.0 37.0 22.0 40.0 68-69 32.156813839023606 35.0 27.0 37.0 22.0 40.0 70-71 32.00649897981131 33.0 27.0 37.0 22.0 40.0 72-73 31.766464400521844 33.0 27.0 37.0 22.0 40.0 74-75 31.40956050088483 33.0 27.0 37.0 18.5 37.0 76-77 30.334770538511812 33.0 27.0 37.0 15.0 37.0 78-79 31.20982026077302 33.0 27.0 37.0 22.0 37.0 80-81 30.8889678032269 33.0 27.0 37.0 15.0 37.0 82-83 30.84625482378597 33.0 27.0 37.0 15.0 37.0 84-85 30.719515375078164 33.0 27.0 37.0 18.5 37.0 86-87 30.506914404632496 33.0 27.0 37.0 15.0 37.0 88-89 30.358847531409104 33.0 27.0 37.0 15.0 37.0 90-91 30.27303853464863 33.0 27.0 37.0 15.0 37.0 92-93 30.0544945602393 33.0 27.0 37.0 15.0 37.0 94-95 29.96608837981413 33.0 27.0 35.0 15.0 37.0 96-97 29.817946144666656 33.0 27.0 33.0 15.0 37.0 98-99 29.564956373127472 33.0 27.0 33.0 15.0 37.0 100-101 28.772217235002145 33.0 27.0 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 1.0777906957102807 2306 2 0.6154767323873394 2306 3 0.5043394078757188 2306 4 0.29733574681742425 2306 5 0.6848213103357779 2306 6 0.7650667013816772 2306 7 0.7430693275866602 2306 8 0.731698453749857 2306 9 1.0116251958153057 2306 10-11 1.1388189496829995 2306 12-13 1.3601068113423338 2306 14-15 1.4981574862914258 2306 16-17 1.4356144322129154 2306 18-19 1.1898961214761812 2306 20-21 1.6022576136483266 2306 22-23 1.256692755297074 2306 24-25 1.225862853966511 2306 26-27 1.342116544370782 2306 28-29 1.5572952811708802 2306 30-31 1.544470659118332 2306 32-33 1.2540046262249618 2306 34-35 1.152103678237495 2306 36-37 1.4594404201780407 2306 38-39 1.1454015771227049 2306 40-41 1.439341325530009 2306 42-43 1.3910210663749538 2306 44-45 1.1250002993071178 2306 46-47 1.248978599250158 2306 48-49 1.2357447951049139 2306 50-51 1.2638715773697484 2306 52-53 1.4198176281562382 2306 54-55 1.8578028098779455 2306 56-57 1.2577975222583007 2306 58-59 1.3397788136396827 2306 60-61 1.1324241051020962 2306 62-63 0.8551162592241468 2306 64-65 0.9635897154691051 2306 66-67 1.2626852643577777 2306 68-69 1.1283509581089994 2306 70-71 0.9485859450642948 2306 72-73 0.6799223188011965 2306 74-75 0.9391922684134073 2306 76-77 0.9849936815924139 2306 78-79 0.9447981733076709 2306 80-81 1.433962382968268 2306 82-83 1.2286022162235888 2306 84-85 1.0083478691042984 2306 86-87 0.8960965772773939 2306 88-89 0.7639188830694863 2306 90-91 0.5215348721452315 2306 92-93 0.3433372144467839 2306 94-95 0.24439189851774046 2306 96-97 0.33059585691116666 2306 98-99 0.48721208094530866 2306 100-101 0.5151632337793188 2307 1 0.732556291962009 2307 2 0.5488198061620935 2307 3 0.3009058335364756 2307 4 0.32871166639936433 2307 5 0.5765586333905759 2307 6 0.6495361556272385 2307 7 0.8343674448199252 2307 8 0.7605244576428802 2307 9 1.04457930367208 2307 10-11 1.1473630938166863 2307 12-13 1.3189728521156354 2307 14-15 1.3608035528155327 2307 16-17 1.3789986319847358 2307 18-19 1.215878335267206 2307 20-21 1.412629186539185 2307 22-23 1.288550547958259 2307 24-25 1.2718027128762373 2307 26-27 1.3255552480987873 2307 28-29 1.5464935873422263 2307 30-31 1.767620283296658 2307 32-33 1.4088119808641508 2307 34-35 1.2740129465602834 2307 36-37 1.4408750268106019 2307 38-39 1.216361278426696 2307 40-41 1.3517641154745164 2307 42-43 1.3258966748788623 2307 44-45 1.0470287021898983 2307 46-47 1.0536223801565185 2307 48-49 1.1753897002608582 2307 50-51 1.0946242219912463 2307 52-53 1.3253342452831092 2307 54-55 1.6968715218438248 2307 56-57 1.3715718099064205 2307 58-59 1.35140297776546 2307 60-61 1.213394725832977 2307 62-63 0.916772222352428 2307 64-65 0.9280905417772729 2307 66-67 1.1492008414010186 2307 68-69 1.0346579104776303 2307 70-71 1.1375112770195983 2307 72-73 1.0605465263083431 2307 74-75 0.9316764460515685 2307 76-77 1.0884904606020633 2307 78-79 1.1221661889261831 2307 80-81 1.794461186238184 2307 82-83 1.4263504443466637 2307 84-85 1.0780637547357301 2307 86-87 1.1168720834226527 2307 88-89 1.0380843874260322 2307 90-91 0.8166619031838387 2307 92-93 0.7901562945814931 2307 94-95 0.7649120884975922 2307 96-97 0.6575496530025369 2307 98-99 0.8218587954404448 2307 100-101 0.8653948582176305 1101 1 1.0754773729157918 1101 2 0.5950447304759408 1101 3 0.40463890288722837 1101 4 0.3683181847099206 1101 5 0.578933174237676 1101 6 0.5782514584019083 1101 7 0.7710175216427402 1101 8 0.70898774706491 1101 9 0.8727407947975792 1101 10-11 0.9529411899209492 1101 12-13 1.216059468855562 1101 14-15 1.32712269431115 1101 16-17 1.2304259958563861 1101 18-19 0.9123810400559904 1101 20-21 1.3061414116851253 1101 22-23 1.033945745041251 1101 24-25 1.1364466600569187 1101 26-27 1.2012558738552457 1101 28-29 1.2715957210729556 1101 30-31 1.3339686966809197 1101 32-33 0.9153237912639938 1101 34-35 1.170951527764906 1101 36-37 1.3296074033766274 1101 38-39 1.039120435784696 1101 40-41 1.056181076632484 1101 42-43 1.1217232865049738 1101 44-45 0.969092732144091 1101 46-47 0.8824870786055854 1101 48-49 0.9770684740920004 1101 50-51 0.8953576991525409 1101 52-53 1.2405819555101445 1101 54-55 1.5854530886531606 1101 56-57 1.289955604014807 1101 58-59 1.1816863051419233 1101 60-61 0.9721246032879165 1101 62-63 0.8152748286563565 1101 64-65 0.8372859605513838 1101 66-67 0.9149748137176843 1101 68-69 0.7395400353524302 1101 70-71 0.8846252776364025 1101 72-73 0.7482597849442243 1101 74-75 0.8785208231749095 1101 76-77 1.0333359148939145 1101 78-79 1.067071385304839 1101 80-81 1.5914153839973224 1101 82-83 1.3731232889423346 1101 84-85 1.1040919235631321 1101 86-87 0.9117440739126224 1101 88-89 0.8865090804931128 1101 90-91 0.8922018514322794 1101 92-93 0.8839080043880685 1101 94-95 0.9207174087452294 1101 96-97 0.7534172845742262 1101 98-99 0.9973920277612969 1101 100-101 0.9447822084154573 2201 1 1.3785306041576746 2201 2 0.7430342277336415 2201 3 0.3825495769862144 2201 4 0.25321492481712227 2201 5 0.3541570652096411 2201 6 0.4675779996631064 2201 7 0.6408193707583152 2201 8 0.7132644613519972 2201 9 0.6639061848638264 2201 10-11 0.725432497468006 2201 12-13 1.0131328229602303 2201 14-15 0.9345082724535914 2201 16-17 0.9465789926318422 2201 18-19 0.9078467991313346 2201 20-21 1.128444373224312 2201 22-23 0.9416681485896703 2201 24-25 0.8843000712073064 2201 26-27 1.0487848986221593 2201 28-29 1.2960973370025854 2201 30-31 1.3322058539618382 2201 32-33 1.0633140316618253 2201 34-35 0.9481544580693324 2201 36-37 0.9944965931801022 2201 38-39 0.8350741063710245 2201 40-41 1.0701514251126127 2201 42-43 1.1303204460176062 2201 44-45 0.8457610245727025 2201 46-47 0.9080551006226543 2201 48-49 0.9840379852246954 2201 50-51 0.9133965683539387 2201 52-53 1.049899286406891 2201 54-55 1.4325396592192376 2201 56-57 1.05437206523181 2201 58-59 1.0357494180896154 2201 60-61 0.8979756816642706 2201 62-63 0.7167957278147554 2201 64-65 0.727942608317143 2201 66-67 0.8097129585746785 2201 68-69 0.7687184036179211 2201 70-71 0.810901260673603 2201 72-73 0.8190273618089208 2201 74-75 0.8613147854014329 2201 76-77 0.8756684465505415 2201 78-79 0.7487150695054972 2201 80-81 1.3709283368154708 2201 82-83 1.2419048066585674 2201 84-85 0.9276773163092606 2201 86-87 1.0585402596144249 2201 88-89 1.0172863661977942 2201 90-91 0.7962310200253917 2201 92-93 0.9001979790389889 2201 94-95 0.8776730746056458 2201 96-97 0.6052413734729924 2201 98-99 0.9106463664420374 2201 100-101 0.8708313050543133 2304 1 -1.6777980426706058 2304 2 -0.13613107347888231 2304 3 -0.04463508303568631 2304 4 0.054901693633077286 2304 5 0.3674172901299144 2304 6 0.48525030665449265 2304 7 0.5249502625264242 2304 8 0.725788673185221 2304 9 0.916852901512776 2304 10-11 0.8997457073376438 2304 12-13 1.2288386095550692 2304 14-15 1.3205947609851094 2304 16-17 1.3115046053544148 2304 18-19 0.9715705457776451 2304 20-21 1.3243031173262167 2304 22-23 1.054235189057728 2304 24-25 1.0842042229871964 2304 26-27 1.1608074226208487 2304 28-29 1.5109375588855514 2304 30-31 1.6507190142754808 2304 32-33 0.9743820033030062 2304 34-35 1.0116240970299373 2304 36-37 1.278196667514095 2304 38-39 1.0815409105156064 2304 40-41 1.0862751072828516 2304 42-43 1.0939457467505562 2304 44-45 1.1417931480470642 2304 46-47 0.9148903642418915 2304 48-49 0.9331631620676646 2304 50-51 0.871356566542218 2304 52-53 1.0899723681769444 2304 54-55 1.5467688903286785 2304 56-57 1.1416152599415739 2304 58-59 0.9414328378795034 2304 60-61 0.6273266846403018 2304 62-63 0.3872676820527232 2304 64-65 0.5078993400726759 2304 66-67 0.6420688265639072 2304 68-69 0.6357981035110747 2304 70-71 0.7101850300197583 2304 72-73 0.5723121116177623 2304 74-75 0.7583638724787747 2304 76-77 0.8151481885396556 2304 78-79 0.6273395176622039 2304 80-81 1.3086969316366037 2304 82-83 1.007887613925714 2304 84-85 0.7781507625556578 2304 86-87 0.5861594004840001 2304 88-89 0.447961593262832 2304 90-91 0.4090535003295521 2304 92-93 0.47022712648938736 2304 94-95 0.24432315600003562 2304 96-97 0.239942758642961 2304 98-99 0.5019003329198064 2304 100-101 0.6038097535254074 1102 1 1.3498656179529576 1102 2 -0.0392574575551663 1102 3 0.21340214326613705 1102 4 0.10164857934513094 1102 5 0.1818042397095212 1102 6 0.1743016351816351 1102 7 -4.532833131163493 1102 8 -0.8875111135507154 1102 9 0.23324520316418074 1102 10-11 0.6292358834200655 1102 12-13 0.8710741974827556 1102 14-15 0.7504506123478762 1102 16-17 0.4633649899740959 1102 18-19 0.786908809369038 1102 20-21 1.0342416689915765 1102 22-23 0.6137782903563007 1102 24-25 0.892682864945499 1102 26-27 0.9581838653168404 1102 28-29 0.792256855746885 1102 30-31 1.0588384942535924 1102 32-33 0.9405969570957993 1102 34-35 1.1185801155053738 1102 36-37 1.2258470410053377 1102 38-39 0.7942202588878473 1102 40-41 1.0907460226062184 1102 42-43 0.9335888551207034 1102 44-45 0.8372930159201175 1102 46-47 0.7097751904856509 1102 48-49 0.6490414712893795 1102 50-51 0.6592541104697744 1102 52-53 0.7986761842264585 1102 54-55 1.2127689052162225 1102 56-57 0.9945508973703454 1102 58-59 0.8379136087564945 1102 60-61 0.8258755213648854 1102 62-63 0.5462675741180334 1102 64-65 0.39977818524594966 1102 66-67 0.5406669782754108 1102 68-69 0.51632389431877 1102 70-71 0.4361598849592525 1102 72-73 0.4041956463671461 1102 74-75 0.44826006908367333 1102 76-77 0.5266856623468854 1102 78-79 0.5814051196214045 1102 80-81 1.0744933593706065 1102 82-83 0.9804906264200817 1102 84-85 0.6714396441661918 1102 86-87 0.5206906847951309 1102 88-89 0.31859870567494397 1102 90-91 -0.007221375824698839 1102 92-93 0.1750145882004901 1102 94-95 0.11546216471296944 1102 96-97 0.17106944791387946 1102 98-99 0.07218926291485417 1102 100-101 0.19887118325062048 2202 1 1.550197172630618 2202 2 0.9137790025999699 2202 3 0.4334077901549733 2202 4 -0.2251671126045096 2202 5 0.06280645959983389 2202 6 0.42534242309409365 2202 7 0.6368106118779195 2202 8 -3.729046724386343 2202 9 -0.3778316722544801 2202 10-11 0.5077695327048275 2202 12-13 -0.02586450862524714 2202 14-15 0.10686311757753231 2202 16-17 0.2655254107701879 2202 18-19 0.7385212304669153 2202 20-21 1.1846888191416625 2202 22-23 -2.5917812374540574 2202 24-25 -2.7952572837482847 2202 26-27 -1.4915754846279583 2202 28-29 0.4966660014206141 2202 30-31 1.2322314457017853 2202 32-33 1.085956277548732 2202 34-35 -1.8764015835451673 2202 36-37 -0.0500075653242078 2202 38-39 0.5080736546798832 2202 40-41 1.2136578739855963 2202 42-43 1.2419924887907925 2202 44-45 1.243064502103529 2202 46-47 0.6653521219159657 2202 48-49 0.9357628807711222 2202 50-51 -3.8232107279309027 2202 52-53 -0.196334063619382 2202 54-55 1.491338676300039 2202 56-57 1.369770314437666 2202 58-59 1.498527244964258 2202 60-61 1.3703157363581795 2202 62-63 1.19486262474571 2202 64-65 1.0362564870184272 2202 66-67 1.2862692063960068 2202 68-69 1.27928527600044 2202 70-71 1.3015305106208217 2202 72-73 1.1478450111493075 2202 74-75 0.6525579916229916 2202 76-77 1.2806700053759492 2202 78-79 1.2183099958356571 2202 80-81 1.8839727596572935 2202 82-83 1.5290781897761185 2202 84-85 1.4057362349999423 2202 86-87 1.451998225334993 2202 88-89 1.4488195630418517 2202 90-91 1.2050228566899506 2202 92-93 1.2679813538591702 2202 94-95 1.0354286606012586 2202 96-97 1.128604729538175 2202 98-99 1.1814523896452123 2202 100-101 1.3039610705186 2305 1 1.3538435851706048 2305 2 0.6191545855057647 2305 3 0.22807620748592683 2305 4 -0.10150191624664018 2305 5 0.24504375871733686 2305 6 0.3694301930530628 2305 7 0.596884874932357 2305 8 -0.12803853489095474 2305 9 0.3697054826357018 2305 10-11 0.10942682303786455 2305 12-13 0.7800525886985952 2305 14-15 0.6074652978087371 2305 16-17 0.5875255680807712 2305 18-19 0.6290169067622315 2305 20-21 0.6710707458691942 2305 22-23 0.5351284896354471 2305 24-25 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-2.7958516374279263 1208 38-39 -0.4667215959326043 1208 40-41 -1.0540563454768588 1208 42-43 -0.2713908225898365 1208 44-45 -0.27212563115225663 1208 46-47 -0.06254739360392847 1208 48-49 -1.3866855683793773 1208 50-51 -0.11844530354039762 1208 52-53 -1.8379070511452973 1208 54-55 -0.7802431635839469 1208 56-57 -6.511857074938742 1208 58-59 -2.1674409264607277 1208 60-61 -1.7624021608955474 1208 62-63 0.10465992332724028 1208 64-65 -0.017714505947196812 1208 66-67 0.6253841321464364 1208 68-69 0.28788840906045365 1208 70-71 -4.337521835657956 1208 72-73 -0.9540039105070903 1208 74-75 -6.140700246344359 1208 76-77 -1.5089224392813705 1208 78-79 -3.905899801724267 1208 80-81 0.9214185193635274 1208 82-83 0.2559597791521462 1208 84-85 -2.7608635519352482 1208 86-87 -0.9686360843711448 1208 88-89 -5.0812601604490695 1208 90-91 -0.3081631416640711 1208 92-93 -2.0509748566663433 1208 94-95 -4.673196900333185 1208 96-97 -3.386040387362371 1208 98-99 -6.10171639341452 1208 100-101 -3.8804353773242397 1209 1 1.6422481859080449 1209 2 1.2425875281066148 1209 3 0.533477671356934 1209 4 0.3682465841110769 1209 5 0.39652413954392784 1209 6 0.7220580066021256 1209 7 0.6425758110393431 1209 8 0.09139352718598559 1209 9 -0.28529149056335257 1209 10-11 -3.101349964150515 1209 12-13 -0.3474665351801036 1209 14-15 -1.541643171730641 1209 16-17 -0.34136662277278873 1209 18-19 -16.884880237038328 1209 20-21 -14.031881760872313 1209 22-23 -4.333596401674882 1209 24-25 -2.501223391365329 1209 26-27 -2.1700957501765146 1209 28-29 -3.0055701970419975 1209 30-31 -0.9630777786168707 1209 32-33 -0.4414196833177968 1209 34-35 -3.3553324011552306 1209 36-37 -4.456323078951044 1209 38-39 -1.6247879805402405 1209 40-41 -10.802834089676182 1209 42-43 -5.401735673333597 1209 44-45 -3.2101675034869714 1209 46-47 -2.8056646495725026 1209 48-49 -3.1014139186698273 1209 50-51 -2.9720314826478855 1209 52-53 -1.2741205765473964 1209 54-55 -3.3443880882003114 1209 56-57 -0.932099007714676 1209 58-59 -2.097035298711493 1209 60-61 -2.008492789148459 1209 62-63 -1.0944368162046771 1209 64-65 -0.4489374333169991 1209 66-67 -0.004681177950608628 1209 68-69 -1.0988069671861425 1209 70-71 -0.8248845837187986 1209 72-73 -0.4909041153356668 1209 74-75 0.3757934912735834 1209 76-77 -17.241403719292116 1209 78-79 -16.881812296267768 1209 80-81 -22.525461202606856 1209 82-83 -9.94619259988146 1209 84-85 -3.4453905993816107 1209 86-87 -2.4650003770409654 1209 88-89 -2.0792202692432653 1209 90-91 -6.683385598935484 1209 92-93 -3.7344819585098 1209 94-95 -0.9795608572184484 1209 96-97 0.1911833458903729 1209 98-99 0.7135802722447941 1209 100-101 0.17738000832433087 1212 1 -28.64293699927714 1212 2 -16.291607131669444 1212 3 -5.858425860081482 1212 4 -1.4241737431929735 1212 5 -0.7809091076567611 1212 6 -3.005116842665746 1212 7 -0.6678893052397257 1212 8 -1.8774695219096245 1212 9 -0.11483498754871135 1212 10-11 0.611311534557494 1212 12-13 0.2047513804099026 1212 14-15 -0.44803852056784876 1212 16-17 -0.11455783724306912 1212 18-19 -1.095668350733419 1212 20-21 -0.6839274111737765 1212 22-23 -0.7197936454302649 1212 24-25 -0.024306940030278668 1212 26-27 -0.6559915296769532 1212 28-29 0.07539448857384912 1212 30-31 -0.9882069775832818 1212 32-33 -0.08498126815845097 1212 34-35 -1.5738078533516102 1212 36-37 -1.587137032439415 1212 38-39 -0.4826691174911488 1212 40-41 -0.28556449449960297 1212 42-43 -0.10479338220525847 1212 44-45 0.9266757351004529 1212 46-47 -2.300625889882582 1212 48-49 -1.7195879066112596 1212 50-51 -0.3742924645600283 1212 52-53 -1.2066571829212123 1212 54-55 -0.6561882604656049 1212 56-57 -1.974131738119496 1212 58-59 -0.2789043770922035 1212 60-61 -0.007373064772927762 1212 62-63 -4.779083672363164 1212 64-65 -4.243834074143869 1212 66-67 -1.6877776465121954 1212 68-69 -0.17962092067451252 1212 70-71 -0.9549879428919255 1212 72-73 -1.6293623410031977 1212 74-75 -0.3109420815085002 1212 76-77 0.7287728526286443 1212 78-79 -1.1870663875683718 1212 80-81 0.4028333710365537 1212 82-83 0.35156795136746055 1212 84-85 -3.49790997922657 1212 86-87 -1.760564545860948 1212 88-89 -1.090266780871172 1212 90-91 -0.04632702873738381 1212 92-93 -2.6079315709129 1212 94-95 -2.3929975497249067 1212 96-97 -0.33530244222331973 1212 98-99 -1.1994688233624409 1212 100-101 -0.37421344559470526 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 1.0 10 5.0 11 10.0 12 31.0 13 156.0 14 534.0 15 1331.0 16 2576.0 17 4056.0 18 6064.0 19 8664.0 20 12104.0 21 16511.0 22 22188.0 23 29681.0 24 38337.0 25 49028.0 26 61274.0 27 76155.0 28 91590.0 29 111626.0 30 133233.0 31 158525.0 32 185442.0 33 215294.0 34 243755.0 35 271566.0 36 291864.0 37 276822.0 38 99746.0 39 206.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 26.51299819151232 23.732051052553214 27.93414996896628 21.82080078696818 2 26.50239007165232 34.64736062344495 20.428219786936488 18.42202951796623 3 16.466448261687365 28.679272703114556 31.974572013436475 22.879707021761604 4 19.828241247843984 29.041412934348344 31.10716008865718 20.02318572915049 5 19.663732188219623 28.50154751455129 30.421968644456975 21.412751652772112 6 19.770982794229145 30.6619226716072 28.396414516325923 21.170680017837732 7 20.00263247712776 30.272158274157963 29.95808797456213 19.767121274152146 8 19.42564663852601 29.712320906435785 30.25040089221875 20.611631562819458 9 19.377273833260393 28.447361668309547 31.28607718555808 20.889287312871982 10-11 20.378237967628003 28.818212921725745 29.546503912591792 21.25704519805446 12-13 20.55041318119357 29.273085664528402 29.044081397298378 21.132419756979647 14-15 20.689111094686965 29.257491971775195 29.022748920642854 21.030648012894986 16-17 20.85600254778234 29.35303933907138 28.660226070008378 21.130732043137908 18-19 20.955408134888387 29.493885885096955 28.67585775730017 20.874848222714487 20-21 20.99754315873304 29.275649119718384 28.681846737450158 21.04496098409842 22-23 20.7371145096647 29.17447534699191 28.998651581822205 21.08975856152119 24-25 20.80339963244972 29.019302617778436 29.17170809565608 21.00558965411576 26-27 20.99134770372425 29.329449114715384 28.746068931039897 20.933134250520474 28-29 20.975777058252483 29.203555255860998 29.061343360552204 20.759324325334312 30-31 21.11005830480099 28.96856307440111 28.83208117662593 21.08929744417197 32-33 21.017734757583735 29.207665906265547 28.731702415484612 21.042896920666106 34-35 21.130279383053658 28.952265798807332 28.926148636136023 20.99130618200299 36-37 21.114459607254343 29.088581609697485 28.76122435929908 21.035734423749094 38-39 20.901702307528137 29.000264908581624 29.21333362121727 20.884699162672966 40-41 21.16400281938399 28.795834453578262 28.85024981029366 21.18991291674409 42-43 20.807634848018044 29.35151790956403 28.993704476622856 20.84714276579507 44-45 20.91945284336595 28.77536250538744 29.23235056955345 21.072834081693156 46-47 21.06579388917315 28.991187777017284 28.9369887432137 21.006029590595865 48-49 20.984755485494468 28.846406570074546 29.219500538641313 20.949337405789674 50-51 21.241002750817458 28.836373073130222 28.853666891576268 21.06895728447605 52-53 20.91867018079637 29.094772507159917 29.021133566339923 20.96542374570379 54-55 20.888342356923943 28.70780715329419 29.351804600474107 21.05204588930776 56-57 21.098885311275545 29.05251562227185 28.793063107147848 21.05553595930476 58-59 20.917084200091853 28.934661127724702 29.227990914361833 20.92026375782161 60-61 21.263519708213472 28.759504487745897 28.82820337361564 21.14877243042499 62-63 21.1230326219873 29.02800470862771 28.875140397243747 20.973822272141245 64-65 21.244425307055778 28.784111224583647 28.986015750006278 20.985447718354294 66-67 21.356467224092977 28.976437655155436 28.6139576098283 21.053137510923296 68-69 21.158641749362975 29.01850901034544 28.826939123462402 20.995910116829187 70-71 21.470839084297086 28.82304139199067 28.572220478247658 21.133899045464585 72-73 21.243311534659973 29.124459299508455 28.701286590420118 20.930942575411454 74-75 21.29014770804839 28.827318771270093 28.773191347504916 21.1093421731766 76-77 21.248083879301237 29.088916317821106 28.524851139358848 21.13814866351881 78-79 21.315793680501535 29.088182616344692 28.519541109002493 21.076482594151276 80-81 21.361701508544538 28.90382145217677 28.402123352302723 21.33235368697597 82-83 21.240262952953444 29.19817536577704 28.32599090589547 21.23557077537404 84-85 21.25177644001939 28.829309158177647 28.495686196347357 21.423228205455604 86-87 21.289949396203045 29.02604144931201 28.188077618372255 21.495931536112693 88-89 21.247694604642994 29.068152085605508 28.1807292928626 21.503424016888896 90-91 21.397275179463964 28.93156385428585 27.975218710351285 21.6959422558989 92-93 21.269265507396835 29.002335842200882 28.071943939787182 21.656454710615105 94-95 21.338556371038052 28.87355513248378 27.98839890957391 21.799489586904254 96-97 21.405402936297175 29.069778064767966 27.67305404389704 21.85176495503782 98-99 21.294147301838834 28.998448156079704 27.85752934986585 21.849875192215613 100-101 21.391628799322284 28.76080607870802 27.636382134711194 22.211182987258507 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 2.0 2 3.0 3 3.5 4 6.5 5 13.0 6 21.0 7 28.0 8 35.0 9 61.0 10 103.0 11 157.5 12 235.0 13 335.5 14 503.5 15 808.0 16 1249.0 17 1835.0 18 2770.5 19 4163.0 20 6004.0 21 8448.0 22 11591.5 23 15373.0 24 19725.5 25 24594.5 26 30226.5 27 36216.0 28 42215.0 29 48328.0 30 54362.0 31 60137.0 32 65636.5 33 71067.0 34 76602.0 35 82094.0 36 86957.0 37 90645.5 38 93243.0 39 94017.0 40 92686.5 41 90472.0 42 87269.0 43 82515.0 44 77547.5 45 73272.5 46 69240.5 47 65594.5 48 62155.5 49 58354.0 50 54652.5 51 51051.0 52 47406.5 53 44292.0 54 41416.0 55 38960.5 56 37148.0 57 35152.5 58 33081.0 59 30722.5 60 27952.5 61 25370.0 62 22870.0 63 20402.5 64 18040.0 65 15804.0 66 13597.0 67 11648.0 68 9963.5 69 8340.5 70 6899.0 71 5719.0 72 4643.0 73 3701.0 74 2903.5 75 2240.0 76 1654.0 77 1159.0 78 833.0 79 604.5 80 405.0 81 256.5 82 179.5 83 124.5 84 83.5 85 55.0 86 38.5 87 26.5 88 18.5 89 15.5 90 11.0 91 5.5 92 2.0 93 1.5 94 2.0 95 1.5 96 1.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7237236015276671 2 0.0034047811431594213 3 3.3217377006433376E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.19560882884663452 14-15 0.0 16-17 3.736954913223755E-4 18-19 0.11273147321558326 20-21 4.152172125804172E-5 22-23 2.076086062902086E-5 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.002096846923531107 42-43 0.0 44-45 0.0 46-47 0.024331728657212448 48-49 1.4532602440314602E-4 50-51 1.2456516377412516E-4 52-53 2.2836946691922946E-4 54-55 0.002532824996740545 56-57 0.0015570645471765644 58-59 0.0989462617579134 60-61 0.0017439122928377523 62-63 0.001370216801515377 64-65 0.0025535858573695657 66-67 0.004463585035239485 68-69 0.004920323969077944 70-71 0.003570868028191588 72-73 0.040213787038413405 74-75 0.0454870456381847 76-77 0.1569521063553977 78-79 2.6989118817727115E-4 80-81 0.11410169001709865 82-83 0.004754237084045777 84-85 0.005003367411594028 86-87 0.006622714540657654 88-89 0.00699641003198003 90-91 0.021300643005375403 92-93 0.010504995478284556 94-95 0.013639885433266706 96-97 0.010089778265704138 98-99 0.011418473345961473 100-101 0.012477277238041536 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2408378.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.63411769795479 #Duplication Level Percentage of deduplicated Percentage of total 1 98.69191264256023 96.35697814779996 2 0.8013631760717423 1.564807733027907 3 0.20549020417045144 0.6018856433926386 4 0.17688671421793975 0.6908071310063528 5 0.06442931227638188 0.3145249528995276 6 0.0193608903090146 0.11341700659005145 7 0.020316499592205044 0.13885084586770582 8 0.0064750632564764985 0.050574967046762315 9 0.00576515336375921 0.0506588095857624 >10 0.007874558685518304 0.10226637740820455 >50 0.0 0.0 >100 1.257854962713531E-4 0.015228385375089647 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.2456516377412516E-4 0.0 0.0 2 1.2456516377412516E-4 0.0 0.0 3 1.6608688503216688E-4 0.0 0.0 4 2.076086062902086E-4 0.0 8.304344251608344E-5 5 2.076086062902086E-4 0.0 1.2456516377412516E-4 6 2.076086062902086E-4 0.0 1.2456516377412516E-4 7 2.9065204880629204E-4 0.0 1.2456516377412516E-4 8 3.736954913223755E-4 0.0 1.2456516377412516E-4 9 4.152172125804172E-4 0.0 1.2456516377412516E-4 10-11 4.152172125804172E-4 0.0 1.2456516377412516E-4 12-13 5.605432369835631E-4 0.0 1.8684774566118774E-4 14-15 7.2663012201573E-4 4.152172125804172E-5 2.2836946691922946E-4 16-17 7.681518432737718E-4 4.152172125804172E-5 3.114129094353129E-4 18-19 8.304344251608344E-4 4.152172125804172E-5 3.3217377006433376E-4 20-21 8.927170070478969E-4 6.228258188706258E-5 3.3217377006433376E-4 22-23 0.0010795647527090846 8.304344251608344E-5 3.3217377006433376E-4 24-25 0.0012456516377412517 8.304344251608344E-5 3.9445635195139636E-4 26-27 0.0016608688503216688 8.304344251608344E-5 4.567389338384589E-4 28-29 0.0025743467179985867 8.304344251608344E-5 4.567389338384589E-4 30-31 0.004131411265175151 8.304344251608344E-5 4.982606550965006E-4 32-33 0.007639996711479677 8.304344251608344E-5 4.982606550965006E-4 34-35 0.0130378204750251 8.304344251608344E-5 4.982606550965006E-4 36-37 0.02175738193921386 8.304344251608344E-5 4.982606550965006E-4 38-39 0.03496128929927113 8.304344251608344E-5 4.982606550965006E-4 40-41 0.05555606304325982 8.304344251608344E-5 4.982606550965006E-4 42-43 0.08424757243256664 1.038043031451043E-4 4.982606550965006E-4 44-45 0.12647516295199507 1.2456516377412516E-4 4.982606550965006E-4 46-47 0.18215579115902902 1.2456516377412516E-4 4.982606550965006E-4 48-49 0.25187076115128104 1.2456516377412516E-4 5.397823763545423E-4 50-51 0.3392947452600879 1.2456516377412516E-4 6.020649582416049E-4 52-53 0.44664915557275475 1.2456516377412516E-4 6.228258188706257E-4 54-55 0.5733942097129272 1.2456516377412516E-4 6.228258188706257E-4 56-57 0.7288515341030353 1.4532602440314602E-4 6.643475401286675E-4 58-59 0.9053811320315996 1.6608688503216688E-4 6.643475401286675E-4 60-61 1.107695718861408 1.6608688503216688E-4 6.643475401286675E-4 62-63 1.3317261659091721 1.6608688503216688E-4 6.643475401286675E-4 64-65 1.5806281239905031 1.6608688503216688E-4 6.643475401286675E-4 66-67 1.8585745260918345 1.6608688503216688E-4 6.643475401286675E-4 68-69 2.162991025495167 1.6608688503216688E-4 6.643475401286675E-4 70-71 2.500251206413611 1.6608688503216688E-4 6.643475401286675E-4 72-73 2.8657046360662655 1.6608688503216688E-4 6.643475401286675E-4 74-75 3.24811968885283 1.8684774566118774E-4 6.643475401286675E-4 76-77 3.6618213586073285 2.076086062902086E-4 6.643475401286675E-4 78-79 4.117854423184401 2.076086062902086E-4 6.643475401286675E-4 80-81 4.603305627272795 2.076086062902086E-4 6.643475401286675E-4 82-83 5.116929319234771 2.2836946691922946E-4 6.851084007576883E-4 84-85 5.657812021202652 2.491303275482503E-4 7.058692613867092E-4 86-87 6.230853296284886 2.491303275482503E-4 7.47390982644751E-4 88-89 6.843838467217355 2.6989118817727115E-4 7.681518432737718E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTTGCG 360 0.0017904403 10.622109 1 CTCGTAT 1560 0.0 10.496076 94-95 TCTCGTA 1665 0.0 9.264066 94-95 TCGTATG 1465 0.0 9.070952 94-95 CGATATG 210 0.006797782 9.040005 40-41 TATGCCG 1040 0.0 8.670853 92-93 TATCTCG 2085 0.0 8.194622 94-95 ATCTCGT 2000 0.0 7.949802 92-93 TGCCGTC 1085 0.0 7.873634 94-95 ATGCCGT 1070 0.0 7.7622347 94-95 CGCCAAT 3540 0.0 7.440844 94-95 CCGTCTT 915 3.6379788E-12 7.26187 92-93 CGTATGC 1265 0.0 6.9408665 94-95 CGTCTTC 935 5.638867E-11 6.8525887 94-95 GCCGTCT 910 2.1645974E-10 6.7800736 94-95 GTCACGC 4940 0.0 6.773212 94-95 ACGCCAA 3895 0.0 6.519103 92-93 GCCAATA 4050 0.0 6.4452553 94-95 AGTCACG 5280 0.0 6.382002 94-95 CGATTCC 2325 3.6379788E-11 6.167677 1 >>END_MODULE