FastQCFastQC Report
Thu 11 Sep 2014
NYCLib4_GCCAAT_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNYCLib4_GCCAAT_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2408378
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGCG3600.001790440310.6221091
CTCGTAT15600.010.49607694-95
TCTCGTA16650.09.26406694-95
TCGTATG14650.09.07095294-95
CGATATG2100.0067977829.04000540-41
TATGCCG10400.08.67085392-93
TATCTCG20850.08.19462294-95
ATCTCGT20000.07.94980292-93
TGCCGTC10850.07.87363494-95
ATGCCGT10700.07.762234794-95
CGCCAAT35400.07.44084494-95
CCGTCTT9153.6379788E-127.2618792-93
CGTATGC12650.06.940866594-95
CGTCTTC9355.638867E-116.852588794-95
GCCGTCT9102.1645974E-106.780073694-95
GTCACGC49400.06.77321294-95
ACGCCAA38950.06.51910392-93
GCCAATA40500.06.445255394-95
AGTCACG52800.06.38200294-95
CGATTCC23253.6379788E-116.1676771
Produced by FastQC (version 0.11.2)