##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename NYCLib3_ACAGTG_L007_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1324693 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.79459467212403 27.0 22.0 33.0 2.0 33.0 2 24.87308757576284 27.0 15.0 33.0 2.0 33.0 3 25.718762762390984 33.0 22.0 33.0 6.0 33.0 4 27.81000579002078 33.0 22.0 37.0 2.0 37.0 5 27.842531061913967 33.0 15.0 37.0 6.0 37.0 6 28.892396955370035 33.0 22.0 37.0 15.0 37.0 7 28.5731607247868 33.0 27.0 37.0 6.0 37.0 8 28.34685621498717 33.0 22.0 37.0 6.0 37.0 9 29.07096512173009 33.0 27.0 37.0 15.0 37.0 10-11 28.69252385269644 33.0 24.5 37.0 10.5 37.0 12-13 28.978992113644445 33.0 27.0 37.0 10.5 37.0 14-15 29.311459711797376 33.0 24.5 37.0 6.0 40.0 16-17 30.112488704930122 33.0 27.0 37.0 15.0 40.0 18-19 30.42683361352404 33.0 27.0 37.0 15.0 40.0 20-21 30.422471470748317 33.0 27.0 37.0 15.0 40.0 22-23 30.42298328744849 33.0 27.0 37.0 15.0 40.0 24-25 30.034793344571156 33.0 27.0 37.0 10.5 40.0 26-27 29.544924371156185 33.0 27.0 37.0 10.5 40.0 28-29 29.69843125916722 33.0 27.0 37.0 15.0 40.0 30-31 29.611351082854668 33.0 27.0 37.0 15.0 40.0 32-33 29.45702966649631 33.0 27.0 37.0 15.0 40.0 34-35 29.486097533541734 33.0 27.0 37.0 10.5 40.0 36-37 29.72940787035185 33.0 27.0 37.0 15.0 40.0 38-39 29.735093716053456 33.0 27.0 37.0 15.0 40.0 40-41 29.80180200242622 33.0 27.0 37.0 15.0 40.0 42-43 29.698710569165836 33.0 27.0 37.0 15.0 40.0 44-45 29.45683037503784 33.0 27.0 37.0 15.0 40.0 46-47 29.08624300120858 33.0 22.0 37.0 10.5 40.0 48-49 28.927779870505844 33.0 22.0 37.0 10.5 40.0 50-51 28.175001302188505 33.0 22.0 37.0 10.5 38.5 52-53 27.90145528058199 33.0 22.0 37.0 6.0 37.0 54-55 28.831279020875026 33.0 22.0 37.0 15.0 40.0 56-57 28.71844495290607 33.0 22.0 37.0 10.5 40.0 58-59 28.896046102757396 33.0 22.0 37.0 15.0 40.0 60-61 28.647264686987853 33.0 22.0 37.0 10.5 40.0 62-63 28.498465682237317 33.0 22.0 37.0 15.0 40.0 64-65 28.432676476738386 33.0 22.0 37.0 10.5 40.0 66-67 28.296373574858478 33.0 22.0 37.0 10.5 40.0 68-69 27.999736542731036 33.0 22.0 37.0 6.0 37.0 70-71 27.900968752760072 33.0 22.0 37.0 6.0 37.0 72-73 27.64064654980437 33.0 22.0 37.0 6.0 37.0 74-75 27.57227259448038 33.0 22.0 37.0 10.5 37.0 76-77 27.16162348559251 27.0 22.0 35.0 6.0 37.0 78-79 26.64090472283012 27.0 22.0 33.0 6.0 37.0 80-81 26.497799490146022 27.0 22.0 33.0 6.0 37.0 82-83 26.061595026168327 27.0 22.0 33.0 6.0 37.0 84-85 26.149134931640766 27.0 22.0 33.0 6.0 37.0 86-87 25.844591539322696 27.0 22.0 33.0 6.0 37.0 88-89 25.472443803960616 27.0 22.0 33.0 6.0 37.0 90-91 25.329289880749727 27.0 22.0 33.0 6.0 37.0 92-93 25.215859070743186 27.0 18.5 33.0 6.0 37.0 94-95 24.99765002155216 27.0 15.0 33.0 6.0 37.0 96-97 24.849373401988235 27.0 15.0 33.0 6.0 37.0 98-99 24.614639391919486 27.0 15.0 33.0 6.0 37.0 100-101 24.129234094239195 27.0 15.0 33.0 6.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 8.106366610410952 2306 2 8.387523451645414 2306 3 7.180959222613552 2306 4 8.809295672367874 2306 5 8.696248163853504 2306 6 7.8647731848502325 2306 7 7.967910511851272 2306 8 8.411257096257323 2306 9 7.387562526713975 2306 10-11 7.666854546019572 2306 12-13 7.225780452009598 2306 14-15 7.923086180376277 2306 16-17 6.751531397523539 2306 18-19 6.113282326093632 2306 20-21 5.981754162610283 2306 22-23 5.6171803361214465 2306 24-25 5.618763811299871 2306 26-27 6.267643533302838 2306 28-29 5.338570391516015 2306 30-31 5.244722843138273 2306 32-33 5.248094910195086 2306 34-35 4.760344482663342 2306 36-37 4.701873187199947 2306 38-39 4.413738801341502 2306 40-41 3.759734679758637 2306 42-43 3.3269328301797145 2306 44-45 2.956630518962939 2306 46-47 3.3383492826307695 2306 48-49 3.030961599587137 2306 50-51 2.8906113928357158 2306 52-53 3.4455620350297416 2306 54-55 3.296314104805578 2306 56-57 3.5074230631723715 2306 58-59 2.9294615187102444 2306 60-61 2.999365588490079 2306 62-63 2.441287695829441 2306 64-65 2.1891482849547614 2306 66-67 2.007753851371266 2306 68-69 2.178930414997801 2306 70-71 2.3264512666057833 2306 72-73 1.4273229939818677 2306 74-75 1.1167308072615967 2306 76-77 1.3888672563359918 2306 78-79 1.7120972137825525 2306 80-81 1.2365051280304549 2306 82-83 1.315965179355043 2306 84-85 1.0333868187303281 2306 86-87 0.9554514298754881 2306 88-89 0.9949809298930781 2306 90-91 0.8482282329213433 2306 92-93 0.7819579343940504 2306 94-95 0.9659684047811545 2306 96-97 0.688582272755081 2306 98-99 0.4394857881515364 2306 100-101 0.42612943935913705 2307 1 8.208253683810991 2307 2 8.383090326832626 2307 3 7.394715460771369 2307 4 8.920410400220575 2307 5 8.791082706582905 2307 6 7.411602466374891 2307 7 8.140124586785923 2307 8 8.443803384297318 2307 9 7.528728106338175 2307 10-11 7.659388725670432 2307 12-13 7.21989131937541 2307 14-15 8.105394658037799 2307 16-17 6.834501565753971 2307 18-19 6.366589942085703 2307 20-21 6.270129820477305 2307 22-23 5.853348463817053 2307 24-25 6.09366793398128 2307 26-27 6.7951169372665206 2307 28-29 5.975389244127825 2307 30-31 5.91304618907056 2307 32-33 5.890712515377338 2307 34-35 5.636676448752937 2307 36-37 5.425846989678529 2307 38-39 4.954512964878639 2307 40-41 4.405622992854568 2307 42-43 4.186094315575488 2307 44-45 4.2113220003245075 2307 46-47 4.529515814535333 2307 48-49 4.279958444364858 2307 50-51 3.8805009456275386 2307 52-53 4.623637713038935 2307 54-55 4.294271088281494 2307 56-57 4.5156365101791245 2307 58-59 4.153174684938573 2307 60-61 4.0600261940365066 2307 62-63 3.751725612055317 2307 64-65 3.4752652492234724 2307 66-67 3.1291095754131106 2307 68-69 3.392423881488785 2307 70-71 3.123630843019935 2307 72-73 2.4552420074424965 2307 74-75 2.219084324898695 2307 76-77 2.41085771852671 2307 78-79 2.712189613834955 2307 80-81 2.4009336472691167 2307 82-83 2.547284295431094 2307 84-85 1.890024299446651 2307 86-87 1.9557131626910156 2307 88-89 2.0389876524153188 2307 90-91 1.9988589022359307 2307 92-93 1.6868649485449367 2307 94-95 1.9510367640828825 2307 96-97 1.6647012743266352 2307 98-99 1.605630605178078 2307 100-101 1.3802989556526555 1101 1 7.947571868084147 1101 2 7.806827495079368 1101 3 7.278193359490384 1101 4 8.636003178312407 1101 5 8.420761518182218 1101 6 7.380904315779649 1101 7 7.1225270966666585 1101 8 8.13639340310462 1101 9 7.1561788466881815 1101 10-11 7.435182995144153 1101 12-13 7.14676997926809 1101 14-15 7.558642548367342 1101 16-17 6.722012832732457 1101 18-19 5.9974756256117345 1101 20-21 5.635561830338229 1101 22-23 5.392048059466045 1101 24-25 5.906439196615004 1101 26-27 6.281113298227503 1101 28-29 5.696645456718755 1101 30-31 5.5199561624618845 1101 32-33 5.642395930985117 1101 34-35 5.253055304880064 1101 36-37 5.071095475312614 1101 38-39 4.717366261917597 1101 40-41 4.271007414358984 1101 42-43 4.047212467923039 1101 44-45 4.066355084134024 1101 46-47 4.525913261927098 1101 48-49 4.090430823314605 1101 50-51 3.557072711629651 1101 52-53 4.658403377889684 1101 54-55 4.345554413633426 1101 56-57 4.373537497592515 1101 58-59 3.937570264802396 1101 60-61 4.36049985721176 1101 62-63 3.921225976319281 1101 64-65 3.5426755664751823 1101 66-67 3.2516953214701196 1101 68-69 3.422892611797831 1101 70-71 3.315705911401942 1101 72-73 2.8572748044016407 1101 74-75 2.2923357678520766 1101 76-77 2.6850279228145837 1101 78-79 3.2069328107403337 1101 80-81 2.8799986710873 1101 82-83 2.9157530185388723 1101 84-85 2.1589295992172914 1101 86-87 2.387722903601521 1101 88-89 2.486235989223264 1101 90-91 2.394795948781301 1101 92-93 2.19660537896452 1101 94-95 2.3339401819230545 1101 96-97 2.0405573546543394 1101 98-99 1.8494210747845408 1101 100-101 1.6344016960938745 2201 1 6.973081987661146 2201 2 7.704630389267589 2201 3 6.069359928339875 2201 4 8.266230726269832 2201 5 3.9485575591670177 2201 6 5.587807808032888 2201 7 6.629892189977049 2201 8 7.81425083486964 2201 9 6.433341356424894 2201 10-11 4.035385950783162 2201 12-13 6.19647820803306 2201 14-15 7.027871244926455 2201 16-17 5.860169273003368 2201 18-19 1.6950672226961814 2201 20-21 4.815395169072513 2201 22-23 5.081756372245767 2201 24-25 5.38138905781063 2201 26-27 5.992307363905439 2201 28-29 4.8165269479086135 2201 30-31 5.2241804242422525 2201 32-33 5.175485321482093 2201 34-35 4.9035859180557395 2201 36-37 4.8859579851693375 2201 38-39 4.198356183747869 2201 40-41 3.9959802595906027 2201 42-43 3.86836972313705 2201 44-45 3.677676492173269 2201 46-47 4.01512108682115 2201 48-49 3.9003191797099888 2201 50-51 3.6174069463212604 2201 52-53 4.517671705407995 2201 54-55 4.092571469828496 2201 56-57 3.991876537927997 2201 58-59 3.7442256734921635 2201 60-61 3.7253054170680677 2201 62-63 3.5071886713685316 2201 64-65 3.3028753775620103 2201 66-67 2.9662583787429746 2201 68-69 3.1204849290087324 2201 70-71 3.1335163789200386 2201 72-73 2.446397054268715 2201 74-75 2.091612940923671 2201 76-77 2.421957267607528 2201 78-79 2.795262801486711 2201 80-81 2.215709921982956 2201 82-83 2.619431364456414 2201 84-85 2.258390584566321 2201 86-87 2.064627488046547 2201 88-89 2.007770170625516 2201 90-91 1.8961880732383563 2201 92-93 1.8540519065747674 2201 94-95 1.702857783853954 2201 96-97 1.6985202129322516 2201 98-99 1.8075036540854654 2201 100-101 1.461811269214916 2304 1 1.5928928120598869 2304 2 4.651657011816667 2304 3 5.699611655824118 2304 4 4.239541143399304 2304 5 6.445219354191707 2304 6 6.307539361073378 2304 7 7.049578144448631 2304 8 6.481104728480773 2304 9 5.728607601402228 2304 10-11 6.60668254803867 2304 12-13 6.527371433332863 2304 14-15 6.654463472903711 2304 16-17 5.368294470304864 2304 18-19 5.184610262678724 2304 20-21 4.845518674939921 2304 22-23 4.540929518196261 2304 24-25 4.6891999757448986 2304 26-27 5.582860119890732 2304 28-29 5.007316114935776 2304 30-31 4.796568269300661 2304 32-33 4.8618061405415105 2304 34-35 4.887921367630348 2304 36-37 4.803957727946642 2304 38-39 4.41036614528138 2304 40-41 3.7414288598704353 2304 42-43 3.4973944204132223 2304 44-45 3.11343995641737 2304 46-47 4.249816687143845 2304 48-49 4.035497111561561 2304 50-51 3.5935409671407115 2304 52-53 4.104338605913476 2304 54-55 3.9987078680092303 2304 56-57 4.50153213232678 2304 58-59 3.861532785699719 2304 60-61 3.7974754802435235 2304 62-63 3.434436193651422 2304 64-65 3.3077655707518403 2304 66-67 3.2789325984033653 2304 68-69 3.364048476655338 2304 70-71 3.2932429433991395 2304 72-73 2.755588244781098 2304 74-75 2.215944103453335 2304 76-77 2.773519427810502 2304 78-79 3.09626293009806 2304 80-81 2.742145166169138 2304 82-83 3.0831434776967583 2304 84-85 2.7103327328435114 2304 86-87 2.5021103690966378 2304 88-89 2.81225364016996 2304 90-91 2.5194170754871053 2304 92-93 2.250197385496147 2304 94-95 2.7400064312912775 2304 96-97 2.6532123836189996 2304 98-99 2.455464774485172 2304 100-101 2.182748183399969 1102 1 6.89366700502579 1102 2 7.236297926222072 1102 3 6.076518585641157 1102 4 6.08772528846907 1102 5 6.213680673886003 1102 6 5.662119378317794 1102 7 6.559544784180304 1102 8 6.141699796217015 1102 9 4.644146805360318 1102 10-11 5.704191407243897 1102 12-13 5.506228065435458 1102 14-15 5.967976142131459 1102 16-17 5.153443237238928 1102 18-19 5.320599446548421 1102 20-21 5.366277880613421 1102 22-23 4.860455071078388 1102 24-25 1.7451845550850038 1102 26-27 5.365046549168319 1102 28-29 4.796682992116729 1102 30-31 4.672610540688023 1102 32-33 4.76565724269436 1102 34-35 4.823013571078327 1102 36-37 4.812368217245631 1102 38-39 3.711240554479609 1102 40-41 3.8167677216487768 1102 42-43 4.147249377731043 1102 44-45 -3.662980428105996 1102 46-47 1.318793353012996 1102 48-49 -0.9126306054494648 1102 50-51 2.6499604488151363 1102 52-53 -0.33278609106784884 1102 54-55 2.5277973899958575 1102 56-57 -0.8721863864592514 1102 58-59 2.7902422985675734 1102 60-61 3.732336283508225 1102 62-63 3.3409878072681884 1102 64-65 2.8354826720650337 1102 66-67 2.8974625797595337 1102 68-69 3.345186066319524 1102 70-71 3.034914929350812 1102 72-73 2.6256681920547216 1102 74-75 2.4303861653797476 1102 76-77 2.887690364200278 1102 78-79 -1.1218394620002066 1102 80-81 1.6117940030739888 1102 82-83 2.4949915643341427 1102 84-85 2.1809208937853626 1102 86-87 2.1663708229588003 1102 88-89 2.1002692233426608 1102 90-91 2.04421978042253 1102 92-93 2.195558141361282 1102 94-95 2.5394368238951373 1102 96-97 2.4802161439848334 1102 98-99 2.5357542278552856 1102 100-101 2.1534650906006405 2202 1 -20.416095951985056 2202 2 -7.569310257357735 2202 3 -0.7597923500381185 2202 4 1.2860482269361881 2202 5 0.7594548207281022 2202 6 1.6521607582399653 2202 7 3.8117862538770595 2202 8 2.496131597068949 2202 9 3.108734616546343 2202 10-11 1.998631741981029 2202 12-13 1.3588809594686673 2202 14-15 2.761928055219297 2202 16-17 1.4362378020097992 2202 18-19 1.4570675171975296 2202 20-21 0.9284204824631281 2202 22-23 1.2601967385186903 2202 24-25 2.151740278627166 2202 26-27 3.4035928995683165 2202 28-29 -0.8163868650349073 2202 30-31 1.0146044197647477 2202 32-33 1.6690850853387786 2202 34-35 1.5026844355345972 2202 36-37 2.8612122467546577 2202 38-39 2.2889689786961362 2202 40-41 1.835459148097108 2202 42-43 0.6293631551469758 2202 44-45 0.6386280365346124 2202 46-47 -1.201106615249902 2202 48-49 0.730465357775536 2202 50-51 1.8730719881470606 2202 52-53 1.467015694335558 2202 54-55 -0.987085976641481 2202 56-57 1.0161877258783178 2202 58-59 1.7939314178443198 2202 60-61 1.9821848109714182 2202 62-63 -2.1307616568615266 2202 64-65 0.15287548770347925 2202 66-67 0.5603981577585166 2202 68-69 1.5409219500310094 2202 70-71 -2.409766338476775 2202 72-73 -4.611602766454858 2202 74-75 -1.4340607817445132 2202 76-77 -0.3540721696491964 2202 78-79 1.4769077762143255 2202 80-81 1.7152081852214067 2202 82-83 -0.4605790430653336 2202 84-85 -0.22683385240318898 2202 86-87 0.36433801931647025 2202 88-89 1.030866243980416 2202 90-91 1.4572480476414782 2202 92-93 1.414740619497124 2202 94-95 1.6502848875687555 2202 96-97 1.0202861501065392 2202 98-99 1.3233690246073024 2202 100-101 1.0192210705519003 2305 1 5.731212829884633 2305 2 7.072330956920698 2305 3 6.625451169463233 2305 4 7.595623306983775 2305 5 7.863765788712293 2305 6 6.103090814108359 2305 7 2.7559623737936754 2305 8 5.784715088052554 2305 9 6.167371752445721 2305 10-11 4.769684107703824 2305 12-13 5.341556959215875 2305 14-15 5.6540188091866135 2305 16-17 5.002936436968344 2305 18-19 4.453355527980946 2305 20-21 5.20572546319681 2305 22-23 5.143750390123355 2305 24-25 5.425325427994736 2305 26-27 6.106255839830048 2305 28-29 5.161123925993486 2305 30-31 5.213166607779822 2305 32-33 5.230131145050748 2305 34-35 5.071305204406613 2305 36-37 4.529879468830028 2305 38-39 4.377674184453376 2305 40-41 3.953029691691846 2305 42-43 3.821055389527608 2305 44-45 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1209 48-49 -19.257159389241007 1209 50-51 -21.590227090644202 1209 52-53 -24.094537798580905 1209 54-55 -21.441970302135235 1209 56-57 -24.93667011486423 1209 58-59 -25.21779305699691 1209 60-61 -24.958704295037375 1209 62-63 -25.054552570393522 1209 64-65 -24.908189485427933 1209 66-67 -12.562275266765173 1209 68-69 -24.599771748063763 1209 70-71 -24.433948067836813 1209 72-73 -14.844788962281502 1209 74-75 -15.599248512919054 1209 76-77 -13.181729368337171 1209 78-79 -14.134904806659566 1209 80-81 -23.45650651922412 1209 82-83 -15.565271450072101 1209 84-85 -12.744073366674947 1209 86-87 -12.991336661400954 1209 88-89 -10.170270436876589 1209 90-91 -13.271417595459923 1209 92-93 -16.752443063942057 1209 94-95 -22.107409299045802 1209 96-97 -18.019359847520647 1209 98-99 -9.502661228725099 1209 100-101 -5.450855347900195 1212 1 -20.416095951985056 1212 2 -20.245421639868727 1212 3 -21.140837782378128 1212 4 -23.070571216151258 1212 5 -23.241522218842 1212 6 -24.26234827937606 1212 7 -23.839410619596315 1212 8 -23.472603136687766 1212 9 -24.42375194915957 1212 10-11 -24.11983430589391 1212 12-13 -24.397347667790736 1212 14-15 -24.696547763051345 1212 16-17 -25.75013249598727 1212 18-19 -20.294715580017908 1212 20-21 -26.144368965509518 1212 22-23 -26.312104189668904 1212 24-25 -22.321586541109035 1212 26-27 -25.057814037412633 1212 28-29 -19.794256918772085 1212 30-31 -14.655791281261141 1212 32-33 -25.199991612267475 1212 34-35 -21.23747189079662 1212 36-37 -25.607278227109536 1212 38-39 -25.881768686179292 1212 40-41 -20.39947001653406 1212 42-43 -16.607324680710374 1212 44-45 -13.466110605380386 1212 46-47 -16.95557657128312 1212 48-49 -19.9184354727081 1212 50-51 -11.935672516005358 1212 52-53 -4.70914454015394 1212 54-55 -3.512977525249198 1212 56-57 -3.401838654190076 1212 58-59 -1.9874559783452241 1212 60-61 -13.620502047846363 1212 62-63 -11.402867177135096 1212 64-65 -15.811560271944787 1212 66-67 -12.115224704967423 1212 68-69 -7.378423433457019 1212 70-71 -14.900240202668273 1212 72-73 -11.211602766454856 1212 74-75 -7.852819941490484 1212 76-77 -18.54288506657152 1212 78-79 -9.134523586755876 1212 80-81 -7.604821125965692 1212 82-83 -4.604496971741444 1212 84-85 -3.9869706379430063 1212 86-87 -3.16463120393707 1212 88-89 -2.7877463598300416 1212 90-91 -3.4336166323780617 1212 92-93 -2.8302520527061006 1212 94-95 -2.622016040618835 1212 96-97 -12.596225818628193 1212 98-99 -9.843411630009207 1212 100-101 -6.306914737948347 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 183.0 3 448.0 4 236.0 5 1317.0 6 1594.0 7 1595.0 8 3425.0 9 6428.0 10 8265.0 11 10484.0 12 14368.0 13 18075.0 14 20726.0 15 22549.0 16 24398.0 17 26932.0 18 29575.0 19 32653.0 20 35118.0 21 38999.0 22 42818.0 23 45603.0 24 47672.0 25 49063.0 26 51593.0 27 53455.0 28 54427.0 29 55093.0 30 57283.0 31 60133.0 32 65174.0 33 73635.0 34 82532.0 35 88863.0 36 91005.0 37 80991.0 38 27878.0 39 107.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 30.433229655778348 27.62959042586012 18.219613916256694 23.717566002104835 2 25.557191874411682 30.390405435952456 23.715827083270074 20.336575606365788 3 20.70387039751624 27.106917559177614 28.03907564108214 24.15013640222401 4 23.01722380758255 26.6179168365267 27.27332178285093 23.091537573039815 5 22.350715321127545 27.03873343383083 27.13002463373954 23.480526611302082 6 22.329986008452135 27.68403732032858 26.520061305385195 23.465915365834086 7 23.255438733060878 27.803365618923582 26.952895639865137 21.988300008150407 8 22.28092778132179 27.49880128918742 27.539828469694505 22.680442459796282 9 22.24074564321983 26.660688292164437 28.234940577978435 22.8636254866373 10-11 23.08667707690473 27.083188616569302 26.86090366701907 22.969230639506893 12-13 22.853162903927473 27.301286094102544 26.840802212231374 23.004748789738606 14-15 22.994195832843232 27.34504982822748 26.777206780045997 22.883547558883297 16-17 23.204589253526375 27.257188706770126 26.691002209706532 22.84721982999697 18-19 23.279100478223523 27.13826211653051 26.597604181357404 22.985033223888564 20-21 23.2869113049509 27.28478561559855 26.51307641052395 22.915226668926607 22-23 23.134185527226894 27.288708560580716 26.672164691786413 22.904941220405973 24-25 23.000997720888655 27.319486460533447 26.84598849479933 22.833527323778572 26-27 22.96581153545983 27.352777812743888 26.781763739583596 22.899646912212685 28-29 23.246164708410397 27.1529818960423 26.82229112146305 22.778562274084248 30-31 23.267948363166013 27.201722666695442 26.51860685073742 23.01172211940112 32-33 23.07700674352839 27.42250380683054 26.555245657105566 22.945243792535504 34-35 23.176471357336204 27.282085832430603 26.718873236403546 22.82256957382965 36-37 23.12091509614989 27.268609837252537 26.68039922032863 22.930075846268945 38-39 23.10862818505262 27.202944526593836 26.891104075077322 22.797323213276226 40-41 23.280910452459764 27.217835099735755 26.57613019546491 22.925124252339568 42-43 23.08702473710072 27.4479181488248 26.625118809796188 22.83993830427829 44-45 23.082611362738756 27.228381561045445 26.84057621160047 22.84843086461533 46-47 23.18056156564367 27.318946838880535 26.64481882833608 22.85567276713971 48-49 23.111843449338508 27.151091856471044 26.841091302762244 22.895973391428207 50-51 23.30235797875275 27.17928362564137 26.55791193364679 22.960446461959087 52-53 23.171780933740248 27.271620455831467 26.687994149822668 22.86860446060561 54-55 23.085057661725546 27.282054940696604 26.681618133851302 22.95126926372654 56-57 23.083814098196935 27.358133904353153 26.779757557101664 22.778294440348247 58-59 23.18436620943098 27.268342708024875 26.699693571514217 22.84759751102993 60-61 23.236516267637132 27.066626400553535 26.604458070361208 23.092399261448126 62-63 23.202179668524565 27.357846632059836 26.606715911929484 22.833257787486115 64-65 23.313408257322198 27.218313247858678 26.542805815291032 22.92547267952809 66-67 23.305517252370223 27.256559330896263 26.58812342386714 22.849799992866373 68-69 23.27942789886694 27.183664466716483 26.735802525474355 22.801105108942217 70-71 23.554244821172635 27.10385694725204 26.462099144246732 22.87979908732859 72-73 23.410156804796312 27.289065970427988 26.48845948126592 22.81231774350978 74-75 23.420569433739853 27.21550981547808 26.49117438152665 22.872746369255413 76-77 23.58270429595195 27.356208384789692 26.310592466410277 22.750494852848085 78-79 23.560221371871197 27.145932989786786 26.559718184829766 22.734127453512247 80-81 23.715211125173337 27.13918274907805 26.351020958101962 22.794585167646648 82-83 23.67091531291399 27.231411587968672 26.382048960758652 22.715624138358688 84-85 23.834495115002976 27.091310865129216 26.195133244530084 22.879060775337724 86-87 23.72084026578294 27.25238498933876 26.27829081682768 22.748483928050618 88-89 23.857892371091964 27.156298750108597 26.347929879165832 22.637878999633607 90-91 24.01402821660026 27.044736472305452 26.097333218496576 22.843902092597713 92-93 24.00302127366797 27.188174087663814 26.10592906055476 22.702875578113456 94-95 24.19667560102209 27.14444430545716 25.92896258752882 22.72991750599193 96-97 24.20153967066577 27.157442215004686 25.90585851316674 22.735159601162803 98-99 24.20779515069114 27.196427222599894 25.95501926127351 22.640758365435456 100-101 24.621752297402292 27.039254239801362 25.604602445569324 22.73439101722702 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 51.0 1 67.0 2 91.5 3 105.5 4 104.0 5 111.5 6 145.0 7 163.5 8 197.5 9 266.0 10 353.5 11 460.0 12 621.0 13 835.0 14 1105.0 15 1440.0 16 1837.5 17 2295.0 18 2884.5 19 3584.5 20 4520.0 21 5700.0 22 7017.5 23 8615.5 24 10333.0 25 12257.0 26 14352.0 27 16546.5 28 18783.0 29 21099.5 30 23571.0 31 26145.5 32 28574.0 33 31087.0 34 33724.0 35 36222.5 36 38552.0 37 40219.5 38 41553.5 39 42156.0 40 41437.5 41 40461.0 42 39117.0 43 37074.0 44 35320.5 45 33906.0 46 32694.5 47 31859.0 48 30710.0 49 29340.5 50 28188.0 51 27112.0 52 26322.5 53 25803.5 54 25341.0 55 25179.0 56 25409.5 57 25473.5 58 25296.5 59 24934.0 60 24310.5 61 23536.0 62 22428.5 63 21054.5 64 19472.0 65 17848.5 66 16135.0 67 14454.5 68 12964.0 69 11107.5 70 9412.0 71 8091.5 72 6790.0 73 5637.5 74 4632.5 75 3738.0 76 2941.5 77 2339.0 78 1873.0 79 1476.5 80 1174.0 81 939.0 82 763.0 83 612.5 84 492.0 85 411.0 86 340.5 87 256.5 88 191.5 89 143.5 90 114.5 91 87.0 92 63.5 93 52.5 94 40.5 95 26.5 96 19.5 97 11.5 98 5.0 99 3.0 100 1.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 13.13587374584149 2 10.500923610225161 3 9.209681035530497 4 11.116009520696494 5 8.70964064881448 6 4.98802364019437 7 9.232252302986428 8 9.47298732612009 9 5.0446405318062375 10-11 5.997200860878709 12-13 5.081932191081254 14-15 6.94183482512552 16-17 2.4830281431244825 18-19 2.849150708881228 20-21 2.907994531563162 22-23 2.972990723133586 24-25 3.266605923032733 26-27 4.047805793493285 28-29 1.6353222973171897 30-31 2.0685547519312024 32-33 2.833335723824313 34-35 1.8474846624840624 36-37 2.8296367535723372 38-39 1.3175883015913876 40-41 1.0836850500455577 42-43 0.5235552690321456 44-45 1.1821606968557998 46-47 1.2611978775459673 48-49 1.158041901029144 50-51 1.4854007683289638 52-53 2.3444677370530376 54-55 1.5959924299441455 56-57 1.6603469634096355 58-59 1.471623991370076 60-61 1.2627831505110996 62-63 0.5746237052660503 64-65 1.166685413148556 66-67 0.5276694298226079 68-69 0.7466258219829047 70-71 0.9271582170359471 72-73 0.07616859151516615 74-75 0.09609773736254362 76-77 0.42379630601203444 78-79 0.7603648543473845 80-81 0.27032678514946484 82-83 0.4794695827637045 84-85 0.05982518213654032 86-87 0.09111545090069925 88-89 0.07382842666187563 90-91 0.08115087797701052 92-93 0.1564135992263868 94-95 0.4248154100610481 96-97 0.3476654590912762 98-99 0.05986292673094823 100-101 0.21695592865667743 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1324693.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.61333053198771 #Duplication Level Percentage of deduplicated Percentage of total 1 99.04834179521981 97.67486868097305 2 0.6991885100462564 1.3789861529071898 3 0.13601047459560522 0.402373376613268 4 0.05755285310751036 0.22701914106199422 5 0.03591879513854359 0.17710360086539761 6 0.0193002549228872 0.1141957450837379 7 0.0036873169693708 0.025453302495373892 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.509783776316475E-4 0.0 0.0 2 3.01956755263295E-4 0.0 0.0 3 6.794026993424137E-4 0.0 7.548918881582374E-5 4 9.058702657898849E-4 0.0 1.509783776316475E-4 5 0.001132337832237356 0.0 1.509783776316475E-4 6 0.0015097837763164749 0.0 1.509783776316475E-4 7 0.001736251342763946 0.0 1.509783776316475E-4 8 0.0018872297203955935 0.0 1.509783776316475E-4 9 0.002113697286843065 0.0 1.509783776316475E-4 10-11 0.0025666324197380075 0.0 1.509783776316475E-4 12-13 0.0033215243078962443 0.0 1.509783776316475E-4 14-15 0.003963182412830746 7.548918881582374E-5 1.509783776316475E-4 16-17 0.0044538621401336 7.548918881582374E-5 1.509783776316475E-4 18-19 0.004906797273028543 7.548918881582374E-5 1.509783776316475E-4 20-21 0.006076879699673811 1.509783776316475E-4 1.509783776316475E-4 22-23 0.006831771587832049 1.509783776316475E-4 1.509783776316475E-4 24-25 0.007473429692766551 1.509783776316475E-4 1.509783776316475E-4 26-27 0.008303810769740612 1.509783776316475E-4 1.509783776316475E-4 28-29 0.009360659413162144 1.509783776316475E-4 1.509783776316475E-4 30-31 0.010643975623031147 1.509783776316475E-4 1.509783776316475E-4 32-33 0.012946395881913772 1.8872297203955936E-4 1.509783776316475E-4 34-35 0.017287024238823636 2.2646756644747123E-4 1.509783776316475E-4 36-37 0.022533522861523388 2.2646756644747123E-4 1.509783776316475E-4 38-39 0.030308909309553232 2.2646756644747123E-4 1.509783776316475E-4 40-41 0.03838625251284637 2.2646756644747123E-4 1.509783776316475E-4 42-43 0.05144588217798388 2.2646756644747123E-4 1.509783776316475E-4 44-45 0.06722312264049105 2.2646756644747123E-4 1.509783776316475E-4 46-47 0.08953017793556696 2.2646756644747123E-4 1.509783776316475E-4 48-49 0.11757441158064548 2.2646756644747123E-4 1.509783776316475E-4 50-51 0.1513935681701345 2.2646756644747123E-4 1.509783776316475E-4 52-53 0.19061020175995497 2.2646756644747123E-4 1.509783776316475E-4 54-55 0.2362434163991204 2.2646756644747123E-4 1.509783776316475E-4 56-57 0.28531138912940585 2.2646756644747123E-4 1.509783776316475E-4 58-59 0.34830711719621077 2.2646756644747123E-4 1.509783776316475E-4 60-61 0.4204747817041382 2.2646756644747123E-4 1.509783776316475E-4 62-63 0.4995119623943057 2.2646756644747123E-4 1.509783776316475E-4 64-65 0.5866264862877664 3.01956755263295E-4 1.509783776316475E-4 66-67 0.6820070763565595 3.01956755263295E-4 2.2646756644747123E-4 68-69 0.7875409623210812 3.01956755263295E-4 2.2646756644747123E-4 70-71 0.9006237671671851 3.01956755263295E-4 2.2646756644747123E-4 72-73 1.017518776048488 3.01956755263295E-4 3.01956755263295E-4 74-75 1.1421514267834132 3.01956755263295E-4 3.01956755263295E-4 76-77 1.2736535937005782 3.01956755263295E-4 3.01956755263295E-4 78-79 1.4226315078286063 3.774459440791187E-4 3.01956755263295E-4 80-81 1.5785166827332824 3.774459440791187E-4 3.01956755263295E-4 82-83 1.7439134954287523 3.774459440791187E-4 3.774459440791187E-4 84-85 1.918670967537384 3.774459440791187E-4 3.774459440791187E-4 86-87 2.1046008395907583 3.774459440791187E-4 3.774459440791187E-4 88-89 2.3035525967148613 3.774459440791187E-4 3.774459440791187E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGATCAA 835 0.008428405 6.713918 1 TCGTGTA 1645 1.2732926E-11 5.1164446 94-95 GTCGTGT 1960 0.0 5.106568 94-95 >>END_MODULE