##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename NYCLib3_ACAGTG_L007_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1324693 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.230487365751912 33.0 33.0 33.0 27.0 33.0 2 31.648393250360648 33.0 33.0 33.0 27.0 33.0 3 31.805650818717996 33.0 33.0 33.0 27.0 33.0 4 35.62052943587684 37.0 37.0 37.0 33.0 37.0 5 35.14700009738105 37.0 37.0 37.0 33.0 37.0 6 34.95177222194123 37.0 33.0 37.0 33.0 37.0 7 34.75698822293165 37.0 33.0 37.0 27.0 37.0 8 34.79284558761917 37.0 33.0 37.0 27.0 37.0 9 34.70226082571585 37.0 33.0 37.0 27.0 37.0 10-11 34.563683434576916 37.0 33.0 37.0 27.0 37.0 12-13 34.58387075345004 37.0 33.0 37.0 27.0 37.0 14-15 35.511300731565726 37.0 33.0 40.0 27.0 40.0 16-17 35.41141532415435 37.0 33.0 40.0 27.0 40.0 18-19 35.6284720308781 37.0 33.0 40.0 27.0 40.0 20-21 35.308092893976195 37.0 33.0 40.0 27.0 40.0 22-23 35.33559851225907 37.0 33.0 40.0 27.0 40.0 24-25 35.17945289965297 37.0 33.0 40.0 27.0 40.0 26-27 34.79931350131691 37.0 33.0 40.0 27.0 40.0 28-29 34.4496577697625 37.0 33.0 40.0 27.0 40.0 30-31 34.34574274945214 37.0 33.0 40.0 27.0 40.0 32-33 34.35632293671062 37.0 33.0 40.0 27.0 40.0 34-35 34.21891298587673 37.0 33.0 40.0 24.5 40.0 36-37 33.91002670052608 37.0 33.0 37.0 22.0 40.0 38-39 33.83079287049905 37.0 33.0 37.0 22.0 40.0 40-41 33.53412564269608 37.0 33.0 37.0 22.0 40.0 42-43 33.168317489410754 37.0 33.0 37.0 22.0 40.0 44-45 33.04495230215605 37.0 33.0 37.0 22.0 40.0 46-47 32.89319185652826 37.0 33.0 37.0 22.0 40.0 48-49 32.91466249161127 37.0 33.0 37.0 22.0 40.0 50-51 33.19304661532898 37.0 33.0 37.0 22.0 40.0 52-53 33.039458198994026 37.0 33.0 37.0 22.0 40.0 54-55 32.66710400070054 37.0 33.0 37.0 22.0 40.0 56-57 32.70672978569374 37.0 33.0 37.0 22.0 40.0 58-59 32.4173249953008 37.0 33.0 37.0 22.0 40.0 60-61 32.29699522832838 37.0 30.0 37.0 22.0 40.0 62-63 32.261626278692496 33.0 30.0 37.0 22.0 40.0 64-65 31.915714810903353 33.0 27.0 37.0 22.0 40.0 66-67 31.558211223279656 33.0 27.0 37.0 18.5 40.0 68-69 31.38232179078473 33.0 27.0 37.0 15.0 40.0 70-71 31.223888100865636 33.0 27.0 37.0 18.5 38.5 72-73 30.995433281522587 33.0 27.0 37.0 15.0 37.0 74-75 30.636650152148462 33.0 27.0 37.0 15.0 37.0 76-77 29.612679315131885 33.0 27.0 35.0 15.0 37.0 78-79 30.516977141118733 33.0 27.0 37.0 15.0 37.0 80-81 30.240628960823376 33.0 27.0 37.0 15.0 37.0 82-83 30.21272287239383 33.0 27.0 37.0 15.0 37.0 84-85 30.097234227100166 33.0 27.0 37.0 15.0 37.0 86-87 29.89357345437773 33.0 27.0 37.0 15.0 37.0 88-89 29.7546423963892 33.0 27.0 37.0 15.0 37.0 90-91 29.6649740732381 33.0 27.0 37.0 15.0 37.0 92-93 29.450793127162292 33.0 27.0 33.0 15.0 37.0 94-95 29.350785427265034 33.0 27.0 33.0 15.0 37.0 96-97 29.215087948679432 33.0 27.0 33.0 15.0 37.0 98-99 28.95167106642822 33.0 27.0 33.0 15.0 37.0 100-101 28.156157690876302 33.0 24.5 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 1.1789281658788902 2306 2 0.5388881464511321 2306 3 0.4901981472556578 2306 4 0.31544765968300226 2306 5 0.6281649377282292 2306 6 0.748731172015745 2306 7 0.8903408766573762 2306 8 0.7958068671943437 2306 9 0.9834877609380328 2306 10-11 1.1166988238087185 2306 12-13 1.3429617610275315 2306 14-15 1.4801092520292656 2306 16-17 1.416554919647595 2306 18-19 1.339665994159006 2306 20-21 1.5402572953111147 2306 22-23 1.0945526022712926 2306 24-25 1.2170904359502899 2306 26-27 1.2988801018314007 2306 28-29 1.5572402922285846 2306 30-31 1.4666421784508117 2306 32-33 1.146269348175224 2306 34-35 1.1001709007743443 2306 36-37 1.1694307243589037 2306 38-39 0.8619673001335073 2306 40-41 1.1124071025071842 2306 42-43 1.111534314386347 2306 44-45 0.9690921051213266 2306 46-47 0.7502177966799337 2306 48-49 0.8622502374218968 2306 50-51 0.8283348319255666 2306 52-53 0.96700272763524 2306 54-55 1.4447310647502931 2306 56-57 1.1295408334860113 2306 58-59 0.9305042151622729 2306 60-61 0.8588200310540373 2306 62-63 0.42909418918323183 2306 64-65 0.5311491816446257 2306 66-67 0.6379378898336228 2306 68-69 0.520841921161086 2306 70-71 0.5132879086288575 2306 72-73 0.46054867564126667 2306 74-75 0.5362069631102031 2306 76-77 0.7465834278964998 2306 78-79 0.7470618833222176 2306 80-81 1.2328840542578163 2306 82-83 1.1616040149860467 2306 84-85 0.7641900397657864 2306 86-87 0.7524286046034021 2306 88-89 0.4754977799373812 2306 90-91 0.1614016466497432 2306 92-93 -0.09697331503180351 2306 94-95 -0.11699924744441859 2306 96-97 -0.014073427053752141 2306 98-99 0.12823132426882822 2306 100-101 0.04056194076497377 2307 1 0.5984170164072324 2307 2 0.392246666157277 2307 3 0.3274411974846956 2307 4 0.2715666958829317 2307 5 0.42077593466189 2307 6 0.7023782038155559 2307 7 0.6776287994915009 2307 8 0.7300096036441133 2307 9 0.9128944671849339 2307 10-11 0.991646866814861 2307 12-13 1.4032272567933433 2307 14-15 1.1490342660104744 2307 16-17 1.279308406580867 2307 18-19 0.9991289772897858 2307 20-21 1.4565265724956902 2307 22-23 1.149270343485341 2307 24-25 1.15381573423047 2307 26-27 1.3092227323068002 2307 28-29 1.4742757799663906 2307 30-31 1.6418527622679306 2307 32-33 1.233292594781787 2307 34-35 1.1269091442961 2307 36-37 1.1573752012193594 2307 38-39 0.8199158689138315 2307 40-41 1.2166722796919345 2307 42-43 1.224119132267056 2307 44-45 0.9829611163599665 2307 46-47 1.0149774209158693 2307 48-49 0.9870838047853354 2307 50-51 0.7775999311964696 2307 52-53 1.0802573737534331 2307 54-55 1.3967550068100962 2307 56-57 1.2532752384521757 2307 58-59 1.2258192266213825 2307 60-61 1.0395907779246372 2307 62-63 0.6811608395745594 2307 64-65 0.8821747875979362 2307 66-67 1.0970794061987057 2307 68-69 0.8864553080298947 2307 70-71 0.8469268951024347 2307 72-73 0.7885688039624874 2307 74-75 1.0213003702712342 2307 76-77 1.1300996279169304 2307 78-79 1.0508886075412462 2307 80-81 1.5285673385054253 2307 82-83 1.171271218969693 2307 84-85 1.0364521327164269 2307 86-87 1.2130871901611755 2307 88-89 1.0479928064367456 2307 90-91 0.7935924166867281 2307 92-93 0.706065325042573 2307 94-95 0.6750698731044622 2307 96-97 0.7211101581721664 2307 98-99 0.7347030793446265 2307 100-101 0.7092490000828064 1101 1 0.8999260413920211 1101 2 0.6033153698216402 1101 3 0.4755397635929022 1101 4 0.37717120003386384 1101 5 0.5692285629434153 1101 6 0.7233380843705817 1101 7 0.727437065823068 1101 8 0.6996988810812326 1101 9 0.8698288648651697 1101 10-11 0.9072503267824317 1101 12-13 1.2366418443949314 1101 14-15 1.3659500364197612 1101 16-17 1.2129418526139943 1101 18-19 0.9472505274339937 1101 20-21 1.3106143703129902 1101 22-23 1.15291352322771 1101 24-25 1.149844956961985 1101 26-27 1.1664209363114182 1101 28-29 1.4290070707810543 1101 30-31 1.3596627497017266 1101 32-33 0.8769733651250533 1101 34-35 1.0535217174639513 1101 36-37 1.106503308114931 1101 38-39 1.0091142121428973 1101 40-41 1.1829757625835313 1101 42-43 1.2272270180117886 1101 44-45 1.1966779050280465 1101 46-47 1.0251177615596845 1101 48-49 0.8696214583973116 1101 50-51 0.8444435089287126 1101 52-53 1.1619684422170522 1101 54-55 1.354780636110597 1101 56-57 1.1184342003658898 1101 58-59 1.172318031811571 1101 60-61 0.9289732934943657 1101 62-63 0.5394051472749979 1101 64-65 0.6448716971752582 1101 66-67 0.9329732691610424 1101 68-69 0.7443310310068441 1101 70-71 0.9404770225048082 1101 72-73 0.7745112754604833 1101 74-75 0.9297851401968344 1101 76-77 0.9170521967880241 1101 78-79 0.8673870046597791 1101 80-81 1.5220448531569986 1101 82-83 1.429926418822788 1101 84-85 1.2652157236029886 1101 86-87 1.0243145616876141 1101 88-89 0.8582474071446313 1101 90-91 0.620011575891084 1101 92-93 0.8017091000894752 1101 94-95 0.8679405011867516 1101 96-97 0.829956131525087 1101 98-99 1.0070621656001713 1101 100-101 0.9186955588985342 2201 1 1.4072966556718924 2201 2 0.8077860348704249 2201 3 0.27645264389468593 2201 4 0.21051917593842973 2201 5 0.4294474573812792 2201 6 0.46200359403407987 2201 7 0.6183155840908725 2201 8 0.6866519900471673 2201 9 0.6073100065536465 2201 10-11 0.81596181652003 2201 12-13 1.1919889633490115 2201 14-15 1.0347435965689158 2201 16-17 1.0481256108819466 2201 18-19 0.8827571423955831 2201 20-21 1.123540100071132 2201 22-23 0.9980389872591928 2201 24-25 0.9378948185176696 2201 26-27 1.0098817680334733 2201 28-29 1.4860690508647565 2201 30-31 1.4587102024344318 2201 32-33 1.1358246794158475 2201 34-35 1.1603812219929495 2201 36-37 1.1169659053470085 2201 38-39 0.7867906521257666 2201 40-41 1.1930857378951956 2201 42-43 1.0146151433665977 2201 44-45 1.016433194056809 2201 46-47 0.902767916926976 2201 48-49 0.7297130104738585 2201 50-51 0.981033854193349 2201 52-53 1.1525778732331347 2201 54-55 1.5221452253324728 2201 56-57 1.1188666363016395 2201 58-59 1.1411851648801417 2201 60-61 0.8665071844456236 2201 62-63 0.7226107181732431 2201 64-65 0.925012109831723 2201 66-67 0.8379743073433481 2201 68-69 0.74684379224772 2201 70-71 0.8800521118905031 2201 72-73 0.8706014088879144 2201 74-75 0.889146364027738 2201 76-77 0.9566691735291393 2201 78-79 0.7822448336945556 2201 80-81 1.419132721786731 2201 82-83 1.2404699702704214 2201 84-85 1.361460804239151 2201 86-87 1.257485386484447 2201 88-89 1.059439216426341 2201 90-91 0.93268364110574 2201 92-93 0.9618752452645651 2201 94-95 0.7610913784808062 2201 96-97 0.6671732868575653 2201 98-99 0.827314941987705 2201 100-101 0.9496236098642861 2304 1 -1.8995343217353842 2304 2 -0.32816863157799503 2304 3 -0.23770777732696757 2304 4 -0.11877919069744536 2304 5 0.33329683400597077 2304 6 0.5280136413084975 2304 7 0.48395975156623194 2304 8 0.6599920267598591 2304 9 0.9102884115390921 2304 10-11 0.855316109605802 2304 12-13 1.2985500128176213 2304 14-15 1.227309237072916 2304 16-17 1.0555502270939883 2304 18-19 0.7401625448516711 2304 20-21 1.195182233148799 2304 22-23 0.9640091260425265 2304 24-25 1.1062657804136364 2304 26-27 1.2139579683086197 2304 28-29 1.4626791675113537 2304 30-31 1.4435331279918415 2304 32-33 0.8987676188476996 2304 34-35 0.8760904356319799 2304 36-37 1.217311597061709 2304 38-39 0.9443554547073418 2304 40-41 0.9865580494308546 2304 42-43 1.0424165518557373 2304 44-45 1.0576564828438464 2304 46-47 0.9600448639274077 2304 48-49 0.8834078586150369 2304 50-51 0.8959251776041199 2304 52-53 1.180648897875848 2304 54-55 1.4238370491583012 2304 56-57 1.189192724529022 2304 58-59 1.0712729713551425 2304 60-61 0.6694091241385891 2304 62-63 0.373453590181569 2304 64-65 0.5636388524180553 2304 66-67 0.6395842096376967 2304 68-69 0.5257726545829158 2304 70-71 0.524981901786326 2304 72-73 0.4273859692904267 2304 74-75 0.7451329105012547 2304 76-77 0.7366008146489165 2304 78-79 0.6565270069925049 2304 80-81 1.2732407631138472 2304 82-83 1.0585648854453922 2304 84-85 1.0560399528320232 2304 86-87 0.8953219097689455 2304 88-89 0.738660299333894 2304 90-91 0.6827929607517049 2304 92-93 0.45366625572106045 2304 94-95 0.2327171428414303 2304 96-97 0.3163474071364547 2304 98-99 0.6820876311730508 2304 100-101 0.613630048226323 1102 1 1.423345766382674 1102 2 0.26571908109935904 1102 3 0.22488060029863277 1102 4 0.14124329024115667 1102 5 0.38633469497626294 1102 6 0.06751752581605786 1102 7 -4.928390860791062 1102 8 -1.2148559560498953 1102 9 0.11716920387932106 1102 10-11 0.607468727190593 1102 12-13 0.9369841116163755 1102 14-15 0.8345757264802529 1102 16-17 0.46870979210137165 1102 18-19 0.7971497379394634 1102 20-21 1.0711961550794484 1102 22-23 0.7220567152101509 1102 24-25 0.7881993217031749 1102 26-27 0.7923595645455421 1102 28-29 0.752854004208551 1102 30-31 0.9593055378310922 1102 32-33 0.8644958342313345 1102 34-35 0.912929200354057 1102 36-37 1.270632952599101 1102 38-39 0.826253152893976 1102 40-41 1.0629889972868156 1102 42-43 1.1291110864596732 1102 44-45 1.1446578055175465 1102 46-47 0.8876514565303637 1102 48-49 0.7577705601196314 1102 50-51 0.5503653435477531 1102 52-53 1.1288353903528687 1102 54-55 1.4679561168764863 1102 56-57 0.8805263678404955 1102 58-59 0.9454811954061313 1102 60-61 0.8637717353582985 1102 62-63 0.6819951731466176 1102 64-65 0.656268625667547 1102 66-67 0.6076168566553584 1102 68-69 0.6479905932884975 1102 70-71 0.6738919374037167 1102 72-73 0.5515685975739757 1102 74-75 0.6366210312663263 1102 76-77 0.48756320647632734 1102 78-79 0.6460177550479926 1102 80-81 1.3300879243310675 1102 82-83 0.90333057357428 1102 84-85 0.5707774269315422 1102 86-87 0.5244767980188207 1102 88-89 0.3209938027037751 1102 90-91 -5.842359926226948E-4 1102 92-93 0.32087099026090016 1102 94-95 0.322887736148612 1102 96-97 0.006735135613666898 1102 98-99 0.05606580931417682 1102 100-101 0.05838986783710354 2202 1 1.6070802361542107 2202 2 0.9269364001757907 2202 3 0.508887239771223 2202 4 -0.3737474369114224 2202 5 0.1662230675184233 2202 6 0.5011841347134762 2202 7 0.7310335180537777 2202 8 -4.078356776366665 2202 9 -0.3686173041423899 2202 10-11 0.31266833237082636 2202 12-13 0.1402305208781911 2202 14-15 0.14741671142563462 2202 16-17 0.25175931356199044 2202 18-19 0.7155020661022817 2202 20-21 1.1787484275796771 2202 22-23 -2.780143292130404 2202 24-25 -2.991340472639614 2202 26-27 -1.485993179712871 2202 28-29 0.710231683388244 2202 30-31 1.2840021069854899 2202 32-33 1.0951818836254148 2202 34-35 -2.0328397060387573 2202 36-37 -0.14145733307996977 2202 38-39 0.3869641503595176 2202 40-41 1.0177842340913301 2202 42-43 1.2320208508298407 2202 44-45 1.3756046997466314 2202 46-47 0.6815299640739596 2202 48-49 1.139831893788461 2202 50-51 -4.013854988492611 2202 52-53 -0.48689384760533727 2202 54-55 1.4297481385574216 2202 56-57 1.4788556458773385 2202 58-59 1.505254407603303 2202 60-61 1.557928909189883 2202 62-63 1.2897115286280751 2202 64-65 1.1257199467023717 2202 66-67 1.2435998422708145 2202 68-69 0.9907848675775384 2202 70-71 1.312182683417987 2202 72-73 1.0018461549279465 2202 74-75 0.4043317380537701 2202 76-77 1.2610658854842889 2202 78-79 1.1918762986388174 2202 80-81 1.87308443678263 2202 82-83 1.661339677824479 2202 84-85 1.5199481111124342 2202 86-87 1.4364259644831634 2202 88-89 1.2790608855576338 2202 90-91 1.1030176798528295 2202 92-93 1.1563628849345449 2202 94-95 1.1053404940871587 2202 96-97 1.0851114520802767 2202 98-99 1.3719459604353865 2202 100-101 1.1294971708447896 2305 1 1.289687224985606 2305 2 0.5549848628146385 2305 3 0.03776664734757418 2305 4 -0.06687374672338109 2305 5 0.29319646371811814 2305 6 0.2743361206455859 2305 7 0.5036367841706593 2305 8 -0.1543515876314956 2305 9 0.4415999095603311 2305 10-11 0.2965565208481209 2305 12-13 0.7624852495790364 2305 14-15 0.5762411316947293 2305 16-17 0.6595185669524568 2305 18-19 0.6598918845934207 2305 20-21 0.6509480075172434 2305 22-23 0.4198744219014614 2305 24-25 0.8836007689018217 2305 26-27 0.8908934915391384 2305 28-29 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-0.6458725179999689 1208 42-43 0.28597604608687277 1208 44-45 0.3564842015526679 1208 46-47 0.1906185313552058 1208 48-49 -1.3458518961454082 1208 50-51 -0.1386606411519793 1208 52-53 -2.094771218205544 1208 54-55 -1.1841025563258896 1208 56-57 -6.315135079681692 1208 58-59 -2.1444086799379534 1208 60-61 -1.3148816627806248 1208 62-63 0.3805082007455809 1208 64-65 0.0604935897260539 1208 66-67 0.7201841311771311 1208 68-69 0.5931131432513936 1208 70-71 -4.234736646089704 1208 72-73 -1.006101803938428 1208 74-75 -6.125145423318266 1208 76-77 -1.4282587107652631 1208 78-79 -4.1644879508097965 1208 80-81 0.9982014825368566 1208 82-83 0.3919017729751886 1208 84-85 -2.519870843542698 1208 86-87 -1.137927665236333 1208 88-89 -5.258288791831248 1208 90-91 -0.32912399175953766 1208 92-93 -2.1701286202006003 1208 94-95 -4.629961380088162 1208 96-97 -3.602338881377115 1208 98-99 -6.8308376401697295 1208 100-101 -4.032825445521983 1209 1 1.942857331555146 1209 2 1.2906114647301905 1209 3 0.4602777764451069 1209 4 0.5425691239092956 1209 5 0.7069324680348643 1209 6 0.9701003186760744 1209 7 0.991327327810211 1209 8 0.21601128839709816 1209 9 -0.38133277626187123 1209 10-11 -2.8734960894311143 1209 12-13 -0.2656072905531701 1209 14-15 -1.1546690935851842 1209 16-17 -0.2866688740296013 1209 18-19 -16.472831034602585 1209 20-21 -14.134368328648954 1209 22-23 -3.3394740200248876 1209 24-25 -1.878405166616858 1209 26-27 -1.1653759000701953 1209 28-29 -3.3009484408353558 1209 30-31 -0.46945242578023283 1209 32-33 -0.7327893634958045 1209 34-35 -2.6626856478351115 1209 36-37 -3.845449237609241 1209 38-39 -0.7717681407982795 1209 40-41 -9.94160406934924 1209 42-43 -4.596339467405905 1209 44-45 -2.421821847814261 1209 46-47 -2.2020819418510875 1209 48-49 -2.2384420611281435 1209 50-51 -2.7648372622032653 1209 52-53 -1.3079586462653978 1209 54-55 -2.4290923248384857 1209 56-57 -0.14002477145813685 1209 58-59 -2.012531201999593 1209 60-61 -1.6081992225708817 1209 62-63 -0.9508321241041457 1209 64-65 -0.11688682976493325 1209 66-67 0.16433140064892982 1209 68-69 -1.4941390629130744 1209 70-71 -0.9963062202040405 1209 72-73 0.19731775099187132 1209 74-75 1.2082268109827936 1209 76-77 -16.561982652445785 1209 78-79 -16.26620172494971 1209 80-81 -21.630358755345224 1209 82-83 -9.817867698824273 1209 84-85 -2.9347224869753603 1209 86-87 -1.7051837082085797 1209 88-89 -1.854036329879591 1209 90-91 -6.211568997514746 1209 92-93 -3.8370761198808694 1209 94-95 -0.7111993900638645 1209 96-97 0.7982334424494582 1209 98-99 1.6890258334202422 1209 100-101 1.4218282825762394 1212 1 -28.635714097016283 1212 2 -16.323674249555523 1212 3 -5.642651597551687 1212 4 -1.0742848086749746 1212 5 -0.24198406487043655 1212 6 -2.7218739991409393 1212 7 -0.36862451809668784 1212 8 -1.2366371867232857 1212 9 -0.09465540868562528 1212 10-11 0.696889143313669 1212 12-13 0.37540394540188515 1212 14-15 -0.00954469551135162 1212 16-17 -0.03456213245657125 1212 18-19 -0.6605116124516996 1212 20-21 -0.2809975421320985 1212 22-23 -0.3636313233956727 1212 24-25 0.6500860051327351 1212 26-27 -0.2691880991071045 1212 28-29 0.6602072573989943 1212 30-31 -0.8016354113340043 1212 32-33 0.240565371656686 1212 34-35 -1.5212129351545371 1212 36-37 -1.0804412119270594 1212 38-39 -0.42485802843872733 1212 40-41 -0.32085768090622224 1212 42-43 0.2338772260130355 1212 44-45 1.1945104154281125 1212 46-47 -1.9055731136006848 1212 48-49 -1.1356932649804712 1212 50-51 0.08199259333445497 1212 52-53 -0.6858679560567325 1212 54-55 -0.20762362499899822 1212 56-57 -1.0110319945721002 1212 58-59 0.19172240955738573 1212 60-61 0.6626675511048816 1212 62-63 -4.1180070839757335 1212 64-65 -3.639057776795429 1212 66-67 -1.7172697229465754 1212 68-69 0.2916153512121298 1212 70-71 -0.8716473116968189 1212 72-73 -0.9878949295859769 1212 74-75 -0.15756371871223251 1212 76-77 0.8035671067837491 1212 78-79 -1.5522370379513148 1212 80-81 0.5491958192936224 1212 82-83 0.748003087692581 1212 84-85 -3.5951960022241565 1212 86-87 -1.7004084273097035 1212 88-89 -0.37032846471105074 1212 90-91 0.23897273603260416 1212 92-93 -2.627906777986805 1212 94-95 -2.2007660032259864 1212 96-97 -0.04747281758264421 1212 98-99 -0.6889236047820049 1212 100-101 -0.1295160191894098 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 14.0 10 18.0 11 31.0 12 55.0 13 117.0 14 419.0 15 910.0 16 1723.0 17 2814.0 18 4218.0 19 6132.0 20 8613.0 21 11685.0 22 15320.0 23 20197.0 24 25119.0 25 31285.0 26 38511.0 27 46682.0 28 55147.0 29 65364.0 30 76740.0 31 89367.0 32 103654.0 33 118241.0 34 132122.0 35 143743.0 36 150229.0 37 131709.0 38 44360.0 39 151.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 28.705781134094583 27.836376515510803 20.273111159753384 23.184731190641227 2 24.97759807588798 29.503579400949526 25.657771070830844 19.861051452331647 3 20.927764231631553 25.977398485683445 29.835584174410616 23.25925310827439 4 22.947220938905044 26.23077666355651 28.62257792754844 22.199424469990007 5 21.739829530313816 27.04339797975833 27.908504083587673 23.308268406340186 6 21.717258262857886 27.79187328686722 27.375701388925584 23.11516706134931 7 22.58266632344249 27.863361548675808 27.810292648938283 21.743679478943424 8 21.87170914317506 27.303760191984104 28.237184011691767 22.58734665314907 9 21.91451151323363 26.53143030120941 28.68536332569131 22.86869485986564 10-11 22.513329503515152 27.05623114185702 27.481084296512474 22.949355058115355 12-13 22.565615502870077 27.156980397583556 27.32496546154492 22.952438638001453 14-15 22.59002651935203 27.246841343616975 27.211323680279133 22.951808456751866 16-17 22.791895148140544 27.084302114913093 27.23822515188482 22.88557758506154 18-19 22.962249250917225 26.981338109326416 27.00295098939382 23.053461650362543 20-21 22.893681781363682 27.083067548481043 27.036754931142536 22.98649573901274 22-23 22.77003049008336 27.1377217211837 27.16863454400378 22.923613244729157 24-25 22.775050521139615 26.98281790573363 27.28688835828377 22.955243214842984 26-27 22.82845912222681 27.100430061908682 27.18093928178076 22.89017153408375 28-29 22.971322412060758 26.967531344998424 27.21868387618867 22.842462366752144 30-31 23.04658513331013 26.9473757315846 26.913481085806296 23.09255804929897 32-33 22.90402380023145 27.067780987745838 26.978364043593494 23.049831168429215 34-35 23.062966287283167 26.985875217880672 26.99282022325173 22.958338271584434 36-37 23.035563711743023 27.018939482582 27.07106476745933 22.874432038215648 38-39 22.972001814760098 26.86569642928588 27.23993408284033 22.922367673113694 40-41 23.08483798934977 26.94773625955524 26.954001989935577 23.013423761159416 42-43 22.894021482713352 27.031508432519836 27.12594540772843 22.948524677038378 44-45 22.89119063813276 26.908423310155637 27.226798963986372 22.97358708772523 46-47 22.930789939328363 27.00610718089253 27.20400445627516 22.859098423503944 48-49 23.090792768887873 26.8140342003899 27.16588944226679 22.929283588455434 50-51 23.07881611636223 26.90887912728327 26.98312285657561 23.02918189977889 52-53 22.968006489062915 26.995588780926568 27.196088444810318 22.840316285200206 54-55 22.9031286424331 26.876287118846744 27.24011522922039 22.980469009499767 56-57 22.989378217513845 27.068277063917535 27.058199184937383 22.88414553363124 58-59 22.980926263759223 26.8659783766263 27.264876316705095 22.888219042909387 60-61 23.133084469137945 26.803240015550866 26.982527921732334 23.08114759357885 62-63 23.065727373471713 26.969073379009632 26.955485232704557 23.009714014814097 64-65 23.133874572395598 26.983720176209058 26.929857969096606 22.952547282298735 66-67 23.13447370745395 26.959588662087352 26.99971237750566 22.90622525295304 68-69 23.033831705853267 26.94231284608329 27.059589550407846 22.964265897655597 70-71 23.243696800208657 26.903491412353848 26.82645303773144 23.02635874970606 72-73 23.058499782699236 26.978258218254545 27.030402850321494 22.93283914872472 74-75 23.04816949572394 26.86584861145096 27.057863510447728 23.028118382377375 76-77 23.039825874022515 27.17425941459288 26.93463963097268 22.851275080411924 78-79 23.169005578663686 26.997901395798262 26.890517781518696 22.942575244019356 80-81 23.115510294148763 26.9412050093858 26.883545080542532 23.059739615922904 82-83 23.15014413235793 27.057627653482935 26.79446389821315 22.99776431594598 84-85 23.073012014539746 26.914682801807277 26.851496061208852 23.160809122444125 86-87 22.980614579397486 27.092103867534277 26.832257623735543 23.09502392933269 88-89 23.07926626725773 27.021819021850728 26.85667781951708 23.042236891374458 90-91 23.131709409066133 26.85726711394802 26.73431004359559 23.276713433390263 92-93 23.009064351877893 26.954263771016496 26.820070746713405 23.21660113039221 94-95 23.16516878363442 26.96030911957093 26.598031310587256 23.276490786207397 96-97 23.087564944428255 27.082034193118798 26.59954672903546 23.230854133417488 98-99 23.02180514845356 26.94378752389922 26.804988024438074 23.22941930320915 100-101 23.197398679408526 26.796599318414145 26.57543120044573 23.430570801731594 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3.0 1 1.5 2 0.5 3 1.0 4 2.5 5 6.5 6 8.0 7 16.5 8 27.5 9 38.5 10 67.5 11 100.5 12 146.5 13 223.5 14 310.5 15 453.5 16 665.0 17 946.5 18 1393.0 19 2063.0 20 2864.5 21 3909.0 22 5208.5 23 6648.0 24 8400.0 25 10294.0 26 12363.5 27 14653.5 28 17114.0 29 19800.5 30 22347.5 31 24840.0 32 27662.5 33 30311.5 34 32984.0 35 35802.0 36 38572.5 37 40877.0 38 42234.0 39 42780.0 40 42425.0 41 41679.0 42 40158.5 43 38044.0 44 36132.0 45 34595.0 46 33471.0 47 32476.5 48 31377.5 49 30030.0 50 28966.0 51 27915.5 52 26706.0 53 26099.0 54 26032.0 55 26120.5 56 26251.5 57 26733.0 58 27188.5 59 27051.5 60 26612.5 61 25566.0 62 24588.5 63 23572.5 64 21799.0 65 19914.5 66 18050.5 67 16141.0 68 14150.0 69 12186.5 70 10274.0 71 8513.0 72 7037.0 73 5638.0 74 4354.5 75 3308.5 76 2402.0 77 1673.5 78 1133.5 79 735.5 80 473.0 81 321.5 82 210.0 83 136.5 84 86.5 85 64.5 86 45.5 87 30.0 88 24.0 89 17.5 90 14.0 91 10.0 92 7.0 93 4.0 94 1.5 95 2.0 96 2.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6767605777338598 2 0.002264675664474712 3 2.2646756644747123E-4 4 7.548918881582374E-5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.17015263159086672 14-15 0.0 16-17 3.774459440791187E-4 18-19 0.10628877785267983 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.002038208098027241 42-43 0.0 44-45 0.0 46-47 0.020268847197048676 48-49 3.774459440791187E-5 50-51 1.509783776316475E-4 52-53 1.8872297203955936E-4 54-55 0.0021891864756588882 56-57 7.171472937503255E-4 58-59 0.08843558469773752 60-61 6.0391351052659E-4 62-63 6.794026993424137E-4 64-65 0.0012455716154610918 66-67 0.0030195675526329497 68-69 0.0036234810631595397 70-71 0.0021514418812509767 72-73 0.03710293630297737 74-75 0.0435572619467303 76-77 0.14890242493921232 78-79 2.2646756644747123E-4 80-81 0.10696818055202224 82-83 0.003208290524672509 84-85 0.0036234810631595397 86-87 0.004793563489804808 88-89 0.005208754028291839 90-91 0.018645829637508465 92-93 0.008190576986516877 94-95 0.011851802644084327 96-97 0.00747342969276655 98-99 0.009096447252306761 100-101 0.010191040490136204 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1324693.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.44284509716915 #Duplication Level Percentage of deduplicated Percentage of total 1 98.53137248525526 96.01177266292204 2 0.9066381007161889 1.7669079201455848 3 0.27088003899267243 0.7918596503843436 4 0.14555320125309368 0.5673247217240918 5 0.07786292085092292 0.37935922676448114 6 0.03471019439460858 0.20293560574118832 7 0.02051589665558288 0.13993891378876452 8 0.006547448287294644 0.0510401591392462 9 0.005837467031332115 0.051193745612453434 >10 0.0 0.0 >50 0.0 0.0 >100 8.22465630356821E-5 0.03766739377780448 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 4.5293513289494246E-4 0.0 0.0 2 6.0391351052659E-4 0.0 0.0 3 0.00120782702105318 0.0 0.0 4 0.0016607621539481224 0.0 0.0 5 0.0021891864756588887 0.0 0.0 6 0.00241565404210636 0.0 0.0 7 0.002868589175001302 0.0 0.0 8 0.003246035119080421 0.0 0.0 9 0.003472502685527892 0.0 0.0 10-11 0.0042651391680940415 0.0 0.0 12-13 0.005208754028291839 3.774459440791187E-5 0.0 14-15 0.005850412133226341 7.548918881582374E-5 0.0 16-17 0.006341091860529194 7.548918881582374E-5 0.0 18-19 0.006945005371055784 7.548918881582374E-5 0.0 20-21 0.007926364825661492 7.548918881582374E-5 0.0 22-23 0.00886997968585929 7.548918881582374E-5 0.0 24-25 0.00947389319638588 7.548918881582374E-5 0.0 26-27 0.010417508056583676 7.548918881582374E-5 0.0 28-29 0.011474356700005208 7.548918881582374E-5 0.0 30-31 0.013248352637177066 7.548918881582374E-5 0.0 32-33 0.016079197217770458 1.1323378322373561E-4 0.0 34-35 0.02094824989639109 1.509783776316475E-4 0.0 36-37 0.02664768365198578 1.509783776316475E-4 0.0 38-39 0.03487600523291057 1.509783776316475E-4 3.774459440791187E-5 40-41 0.04431215383488854 1.509783776316475E-4 7.548918881582374E-5 42-43 0.05940999159805328 2.2646756644747123E-4 7.548918881582374E-5 44-45 0.0772631847529956 2.2646756644747123E-4 7.548918881582374E-5 46-47 0.10092904544675635 2.2646756644747123E-4 7.548918881582374E-5 48-49 0.13018110611288802 2.2646756644747123E-4 7.548918881582374E-5 50-51 0.16830314646487904 2.2646756644747123E-4 7.548918881582374E-5 52-53 0.2104261138241087 2.2646756644747123E-4 7.548918881582374E-5 54-55 0.25741813386195894 2.2646756644747123E-4 7.548918881582374E-5 56-57 0.310109587655404 2.2646756644747123E-4 7.548918881582374E-5 58-59 0.37495480084819655 2.2646756644747123E-4 7.548918881582374E-5 60-61 0.4499910545311253 2.2646756644747123E-4 7.548918881582374E-5 62-63 0.5320855473683337 2.2646756644747123E-4 1.1323378322373561E-4 64-65 0.6222573834088352 2.2646756644747123E-4 1.509783776316475E-4 66-67 0.7205065626526297 2.2646756644747123E-4 1.509783776316475E-4 68-69 0.8286448256312973 2.2646756644747123E-4 1.509783776316475E-4 70-71 0.9481442115267462 2.642121608553831E-4 1.509783776316475E-4 72-73 1.075381239275817 3.01956755263295E-4 2.2646756644747123E-4 74-75 1.211224034549892 3.01956755263295E-4 3.01956755263295E-4 76-77 1.3580127622022613 3.01956755263295E-4 3.01956755263295E-4 78-79 1.5181630762750313 3.01956755263295E-4 3.01956755263295E-4 80-81 1.6853338849076729 3.01956755263295E-4 3.01956755263295E-4 82-83 1.8638658164570963 3.01956755263295E-4 3.01956755263295E-4 84-85 2.053645637140077 3.01956755263295E-4 3.01956755263295E-4 86-87 2.2546356023622076 3.01956755263295E-4 3.3970134967120684E-4 88-89 2.4710253621027665 3.01956755263295E-4 3.774459440791187E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGCCG 565 1.6283793E-6 7.1405964 46-47 CTCGTAT 605 6.3276093E-7 7.061422 92-93 CGTATGC 710 2.46946E-8 7.019981 94-95 TCGTATG 710 2.46946E-8 7.019981 94-95 TCTCGTA 630 1.2404143E-6 6.779926 40-41 GTATGCC 760 6.264727E-7 6.2452583 44-45 GCCGTCT 650 1.4542338E-5 6.20542 48-49 CGTCTTC 910 4.3386535E-7 5.7361026 50-51 CCGTCTT 805 1.0984169E-5 5.6000686 50-51 ATCTCGT 910 1.5046169E-5 5.2174945 90-91 ATGCCGT 785 2.5379448E-4 5.1394095 46-47 >>END_MODULE