FastQCFastQC Report
Thu 11 Sep 2014
NYCLib3_ACAGTG_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNYCLib3_ACAGTG_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1324693
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5651.6283793E-67.140596446-47
CTCGTAT6056.3276093E-77.06142292-93
CGTATGC7102.46946E-87.01998194-95
TCGTATG7102.46946E-87.01998194-95
TCTCGTA6301.2404143E-66.77992640-41
GTATGCC7606.264727E-76.245258344-45
GCCGTCT6501.4542338E-56.2054248-49
CGTCTTC9104.3386535E-75.736102650-51
CCGTCTT8051.0984169E-55.600068650-51
ATCTCGT9101.5046169E-55.217494590-91
ATGCCGT7852.5379448E-45.139409546-47
Produced by FastQC (version 0.11.2)