##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename NYCLib2_TGACCA_L007_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2185561 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.085040408389425 33.0 22.0 33.0 2.0 33.0 2 25.09988739733185 33.0 15.0 33.0 2.0 33.0 3 25.996438900584334 33.0 22.0 33.0 2.0 33.0 4 28.19113902563232 33.0 22.0 37.0 2.0 37.0 5 28.281943629118565 33.0 22.0 37.0 6.0 37.0 6 29.366809711556897 37.0 27.0 37.0 15.0 37.0 7 29.031472011076332 37.0 27.0 37.0 6.0 37.0 8 28.774763550411084 37.0 27.0 37.0 2.0 37.0 9 29.582564842619355 37.0 27.0 37.0 15.0 37.0 10-11 29.216959169750925 33.0 27.0 37.0 10.5 37.0 12-13 29.521037161625777 33.0 27.0 37.0 15.0 37.0 14-15 29.918218937837928 33.0 27.0 37.0 6.0 40.0 16-17 30.859896383582978 33.0 27.0 37.0 15.0 40.0 18-19 31.199184099643066 33.0 27.0 37.0 15.0 40.0 20-21 31.23477267392674 33.0 27.0 37.0 15.0 40.0 22-23 31.300938980884084 33.0 27.0 37.0 15.0 40.0 24-25 30.889081338841606 33.0 27.0 37.0 15.0 40.0 26-27 30.3929167385399 33.0 27.0 37.0 15.0 40.0 28-29 30.651857577985698 33.0 27.0 37.0 15.0 40.0 30-31 30.58578621232718 33.0 27.0 37.0 15.0 40.0 32-33 30.44477779389365 33.0 27.0 37.0 15.0 40.0 34-35 30.501472162067312 33.0 27.0 37.0 15.0 40.0 36-37 30.740871108150266 33.0 27.0 37.0 15.0 40.0 38-39 30.831607308146513 33.0 27.0 37.0 15.0 40.0 40-41 30.95486033105459 33.0 27.0 37.0 15.0 40.0 42-43 30.900003706142265 33.0 27.0 37.0 15.0 40.0 44-45 30.670633306505742 33.0 27.0 37.0 15.0 40.0 46-47 30.292396551732026 33.0 27.0 37.0 15.0 40.0 48-49 30.175497961392978 33.0 27.0 37.0 15.0 40.0 50-51 29.415788440588024 33.0 24.5 37.0 15.0 38.5 52-53 29.111017491618856 33.0 27.0 37.0 15.0 38.5 54-55 30.093443285270922 33.0 27.0 37.0 15.0 40.0 56-57 30.02289823985695 33.0 27.0 37.0 15.0 40.0 58-59 30.238111404806364 33.0 27.0 37.0 15.0 40.0 60-61 30.011818475897037 33.0 27.0 37.0 15.0 40.0 62-63 29.902959011439165 33.0 27.0 37.0 15.0 40.0 64-65 29.87201684144254 33.0 27.0 37.0 15.0 40.0 66-67 29.746726126610056 33.0 27.0 37.0 15.0 40.0 68-69 29.463546659187273 33.0 27.0 37.0 15.0 40.0 70-71 29.348691480127986 33.0 27.0 37.0 15.0 40.0 72-73 29.099996522631947 33.0 27.0 37.0 15.0 37.0 74-75 29.052695394912334 33.0 27.0 37.0 15.0 37.0 76-77 28.634893283692378 33.0 27.0 37.0 15.0 37.0 78-79 28.0191955292028 33.0 22.0 37.0 10.5 37.0 80-81 27.883455552144277 33.0 22.0 37.0 15.0 37.0 82-83 27.42002648290302 30.0 22.0 33.0 10.5 37.0 84-85 27.50060167618291 33.0 22.0 33.0 15.0 37.0 86-87 27.156740534810055 27.0 22.0 33.0 10.5 37.0 88-89 26.751486002907264 27.0 22.0 33.0 6.0 37.0 90-91 26.583117103572036 27.0 22.0 33.0 6.0 37.0 92-93 26.430749130314826 27.0 22.0 33.0 6.0 37.0 94-95 26.168255656099284 27.0 22.0 33.0 6.0 37.0 96-97 25.986934018313832 27.0 22.0 33.0 6.0 37.0 98-99 25.702915635848186 27.0 22.0 33.0 6.0 37.0 100-101 25.162591435333994 27.0 18.5 33.0 6.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 8.35795630187268 2306 2 8.626452384565788 2306 3 7.481038301462668 2306 4 9.007575007796351 2306 5 8.8614258483049 2306 6 7.961653233291109 2306 7 8.210722653044236 2306 8 8.641619813645622 2306 9 7.582671143634315 2306 10-11 7.768863070553845 2306 12-13 7.36411398790278 2306 14-15 8.09415867704626 2306 16-17 6.900131424296916 2306 18-19 6.352054058653618 2306 20-21 6.312956304270646 2306 22-23 5.8561156141027375 2306 24-25 5.92455919638391 2306 26-27 6.709479219053922 2306 28-29 5.701603817095627 2306 30-31 5.479674063654276 2306 32-33 5.61380300665753 2306 34-35 5.010954753797677 2306 36-37 4.994031213953637 2306 38-39 4.714337393690453 2306 40-41 3.977980703851564 2306 42-43 3.7428982085069045 2306 44-45 3.5580977773335896 2306 46-47 3.564482859108093 2306 48-49 3.3933307086724867 2306 50-51 3.2890729872328066 2306 52-53 3.868679370010664 2306 54-55 3.4911896538647333 2306 56-57 3.833248484831291 2306 58-59 3.358501528536948 2306 60-61 3.549990769699498 2306 62-63 2.931514256004661 2306 64-65 2.523726861192536 2306 66-67 2.3819582372462023 2306 68-69 2.516525063769631 2306 70-71 2.5241133030617675 2306 72-73 1.9026221502388623 2306 74-75 1.4967874272189405 2306 76-77 1.9113868002668681 2306 78-79 2.0154316185412604 2306 80-81 1.7930554376895813 2306 82-83 1.7878997834424588 2306 84-85 1.522855857643421 2306 86-87 1.4453222045690097 2306 88-89 1.3065936785557426 2306 90-91 1.1632133585258586 2306 92-93 1.1136506425959496 2306 94-95 1.506214951891124 2306 96-97 0.9107848778592675 2306 98-99 0.9474079654649685 2306 100-101 0.611416606766781 2307 1 8.333437791654593 2307 2 8.451557787056228 2307 3 7.59980876915558 2307 4 9.257327971438908 2307 5 9.090568994925253 2307 6 7.648117825991605 2307 7 8.183099683472058 2307 8 8.679662245443321 2307 9 7.578412836330614 2307 10-11 7.874608311683925 2307 12-13 7.462306317683012 2307 14-15 8.302108312631454 2307 16-17 6.9469650541112316 2307 18-19 6.46629800880746 2307 20-21 6.572238049913306 2307 22-23 6.153430687009045 2307 24-25 6.285604466928646 2307 26-27 6.927705889269017 2307 28-29 6.1336710132259675 2307 30-31 6.051861533014009 2307 32-33 6.267288245639872 2307 34-35 5.909478115098267 2307 36-37 5.821868373525778 2307 38-39 5.331696355119913 2307 40-41 4.809150636478016 2307 42-43 4.622792292427366 2307 44-45 4.586396863611892 2307 46-47 4.987978953496434 2307 48-49 4.550596220230581 2307 50-51 4.349799085496407 2307 52-53 4.9645970603537855 2307 54-55 4.566527035213976 2307 56-57 4.960894846052515 2307 58-59 4.529968152248891 2307 60-61 4.400060781231904 2307 62-63 4.094999494987004 2307 64-65 3.9198210627715895 2307 66-67 3.749738831982384 2307 68-69 3.897760525696338 2307 70-71 3.5318813148649824 2307 72-73 2.949107496608022 2307 74-75 2.584467591940971 2307 76-77 2.8585120553105483 2307 78-79 3.266989474353455 2307 80-81 2.971577304912792 2307 82-83 3.0102887431443897 2307 84-85 2.4048790718707735 2307 86-87 2.347230514907558 2307 88-89 2.4390879006783557 2307 90-91 2.3552770295673717 2307 92-93 2.116091498142012 2307 94-95 2.3457226067032195 2307 96-97 2.0803131696152306 2307 98-99 2.037020018934264 2307 100-101 1.6608834779352897 1101 1 8.130302737742376 1101 2 7.880909281197223 1101 3 7.270815364164363 1101 4 8.732200933619534 1101 5 8.731310819686453 1101 6 7.381696322789658 1101 7 7.188020405177909 1101 8 8.102462647935983 1101 9 7.424111775367052 1101 10-11 7.546195127846229 1101 12-13 7.324858206420171 1101 14-15 7.836942896673257 1101 16-17 6.970427317849207 1101 18-19 6.0802981615875815 1101 20-21 5.811838057809812 1101 22-23 5.491962979263118 1101 24-25 6.081827035528782 1101 26-27 6.6170102647602285 1101 28-29 5.86635836414732 1101 30-31 5.6583565051459495 1101 32-33 5.754512365215785 1101 34-35 5.611620098190031 1101 36-37 5.416896896774244 1101 38-39 5.1592547284292785 1101 40-41 4.556147319661527 1101 42-43 4.428355548978669 1101 44-45 4.3853376452910915 1101 46-47 4.610241964769838 1101 48-49 4.349471054683008 1101 50-51 3.946030323081235 1101 52-53 4.76875625610089 1101 54-55 4.585919096197852 1101 56-57 4.6947454288056925 1101 58-59 4.122483885883888 1101 60-61 4.380711779066377 1101 62-63 3.990089759717204 1101 64-65 3.6718847388960327 1101 66-67 3.526202552853661 1101 68-69 3.7030613523398195 1101 70-71 3.5635391308702182 1101 72-73 2.9655268540216753 1101 74-75 2.4114152023768263 1101 76-77 2.6730294718157808 1101 78-79 3.2861222525856633 1101 80-81 2.9296847940286384 1101 82-83 2.97381742332151 1101 84-85 2.2436206500093654 1101 86-87 2.4209919873646477 1101 88-89 2.3585520315382524 1101 90-91 2.2933368478505756 1101 92-93 1.9754294251789162 1101 94-95 2.069620594211301 1101 96-97 2.066995406121727 1101 98-99 1.9581998505842968 1101 100-101 1.6787614707019785 2201 1 7.168112385721514 2201 2 7.918718929176361 2201 3 6.4205328782886575 2201 4 8.645934093115404 2201 5 4.010102649325738 2201 6 5.788910577461543 2201 7 6.892062126168781 2201 8 7.844314016906001 2201 9 6.555141762329242 2201 10-11 4.361275527672394 2201 12-13 6.527161552791309 2201 14-15 7.224491788459439 2201 16-17 6.129054001899618 2201 18-19 2.19144548883661 2201 20-21 5.1899345376012 2201 22-23 5.257825647668167 2201 24-25 5.513921776945558 2201 26-27 6.336045466846276 2201 28-29 5.189478746589746 2201 30-31 5.31394103361183 2201 32-33 5.43584656773643 2201 34-35 5.169638351123538 2201 36-37 5.223937313984155 2201 38-39 4.648824480710896 2201 40-41 4.31918512930951 2201 42-43 4.073401181312438 2201 44-45 3.983990404960803 2201 46-47 4.535491856121421 2201 48-49 4.3542515848872725 2201 50-51 3.946790861977366 2201 52-53 4.790594587261257 2201 54-55 4.387092499066167 2201 56-57 4.606631565268106 2201 58-59 4.19506102625764 2201 60-61 4.192967320135484 2201 62-63 3.882745126948837 2201 64-65 3.6074562295522696 2201 66-67 3.3029300626320186 2201 68-69 3.6781808655949213 2201 70-71 3.4840084920175443 2201 72-73 2.7312808740129846 2201 74-75 2.5587424513989916 2201 76-77 2.942151592632815 2201 78-79 3.059047067883359 2201 80-81 2.659258892246804 2201 82-83 2.947850452439287 2201 84-85 2.4931860563544355 2201 86-87 2.4163201055741226 2201 88-89 2.4745885374052676 2201 90-91 2.3329935188631765 2201 92-93 2.0645211244401054 2201 94-95 2.3374389340635346 2201 96-97 2.141636866356407 2201 98-99 2.0977044138176737 2201 100-101 1.7358018879118902 2304 1 1.7295063317795396 2304 2 5.082260012239601 2304 3 5.99999754268287 2304 4 4.629302769908627 2304 5 6.754181267143377 2304 6 6.420017185554059 2304 7 7.311760563551516 2304 8 6.570258909034269 2304 9 5.995612554668526 2304 10-11 6.846584167708418 2304 12-13 6.755075991947333 2304 14-15 7.023159645458925 2304 16-17 5.622945517549354 2304 18-19 5.403963967793718 2304 20-21 5.013484801237237 2304 22-23 4.7208538578579 2304 24-25 4.821735561418521 2304 26-27 5.832213275848471 2304 28-29 5.13973705928424 2304 30-31 5.086656318916457 2304 32-33 5.077341273198865 2304 34-35 4.747728373097573 2304 36-37 5.0000919106411 2304 38-39 4.693720885102909 2304 40-41 3.989427768706733 2304 42-43 3.6050613290742852 2304 44-45 3.318850575761342 2304 46-47 4.250445717634403 2304 48-49 4.05368190067454 2304 50-51 3.603630650510027 2304 52-53 4.192701842880869 2304 54-55 3.9759267583108873 2304 56-57 4.639735995156283 2304 58-59 3.9878971251076294 2304 60-61 3.8905130004665445 2304 62-63 3.6126830446343625 2304 64-65 3.4542933825973066 2304 66-67 3.2443148979314955 2304 68-69 3.551317567116353 2304 70-71 3.4742399087754023 2304 72-73 3.0625308631045094 2304 74-75 2.4579270210552764 2304 76-77 2.771486489805163 2304 78-79 3.1628584891442415 2304 80-81 2.851626886605814 2304 82-83 3.1130185564066544 2304 84-85 2.6348029367399484 2304 86-87 2.5520162146518928 2304 88-89 2.7016143285315266 2304 90-91 2.7076081006147845 2304 92-93 2.548720852513636 2304 94-95 2.783317973908268 2304 96-97 2.658510532249881 2304 98-99 2.426946545316291 2304 100-101 1.9898755489183664 1102 1 7.115127960128454 1102 2 7.574656934054794 1102 3 6.249571763283985 1102 4 6.350548659306501 1102 5 6.601525511023947 1102 6 5.99611928963985 1102 7 6.85136323326892 1102 8 6.514686280817788 1102 9 4.791803596148707 1102 10-11 6.032496829080966 1102 12-13 5.7494370675568796 1102 14-15 6.198348441963979 1102 16-17 5.348120671881418 1102 18-19 5.3958697713815305 1102 20-21 5.480735448642111 1102 22-23 5.108617706259057 1102 24-25 1.7552493543734187 1102 26-27 5.446417570181151 1102 28-29 4.886127946631806 1102 30-31 4.972849322954861 1102 32-33 5.0244946862152595 1102 34-35 4.9120463522051026 1102 36-37 4.934622723602917 1102 38-39 3.9040027754772773 1102 40-41 4.010540108411199 1102 42-43 4.06731798382199 1102 44-45 -4.1012689966881695 1102 46-47 1.4143469323462448 1102 48-49 -0.8913042417497508 1102 50-51 2.686305888609823 1102 52-53 -0.05192050826387984 1102 54-55 2.7812207192160905 1102 56-57 -0.8088484897838804 1102 58-59 2.9519245498642626 1102 60-61 3.8572185991824206 1102 62-63 3.482718450461082 1102 64-65 3.054042079410781 1102 66-67 3.179761092722579 1102 68-69 3.6002318575717744 1102 70-71 3.3073891126628645 1102 72-73 2.8503105077059594 1102 74-75 2.6220315121224473 1102 76-77 2.877708916261085 1102 78-79 -1.384893743465181 1102 80-81 1.733284306910793 1102 82-83 2.7512075459763743 1102 84-85 2.4402030034350197 1102 86-87 2.4039593866246456 1102 88-89 2.3417028510762172 1102 90-91 2.127601941692241 1102 92-93 2.2246404227293404 1102 94-95 2.469602723554452 1102 96-97 2.531632898552594 1102 98-99 2.3397529557015737 1102 100-101 1.7881202559945848 2202 1 -20.951618166212427 2202 2 -7.932606556444194 2202 3 -0.6798535634404423 2202 4 1.834407901058043 2202 5 1.418923198685654 2202 6 2.1299221282926695 2202 7 4.108976416116203 2202 8 2.9543619398348895 2202 9 3.5372657978360316 2202 10-11 2.3952084951361847 2202 12-13 1.6380381505924682 2202 14-15 2.795049469849104 2202 16-17 1.8229825983998715 2202 18-19 1.845675337699877 2202 20-21 1.3933377575485153 2202 22-23 1.5431842775306386 2202 24-25 2.5234652558946706 2202 26-27 3.7304876672040876 2202 28-29 -0.8886450939568071 2202 30-31 1.0827767831131965 2202 32-33 1.7190068894984094 2202 34-35 1.6771107842085655 2202 36-37 3.1594551530338393 2202 38-39 2.515042682514263 2202 40-41 1.8847992922324508 2202 42-43 0.7617078402557773 2202 44-45 0.6787319093305761 2202 46-47 -1.4387436726168836 2202 48-49 0.7251079446141873 2202 50-51 1.7687287817816362 2202 52-53 1.7044452171843005 2202 54-55 -0.926608037750217 2202 56-57 1.049841105105628 2202 58-59 1.8979943332125302 2202 60-61 2.1192968757700363 2202 62-63 -2.129066485398628 2202 64-65 -0.09093677500401753 2202 66-67 0.4940804444249842 2202 68-69 1.4335449313193003 2202 70-71 -2.66118051367647 2202 72-73 -4.65884004137915 2202 74-75 -1.1917322167212419 2202 76-77 -0.2008635688419389 2202 78-79 1.6183593099724227 2202 80-81 1.6285126425097438 2202 82-83 -0.8231299535560304 2202 84-85 -0.3176208287703126 2202 86-87 0.5609802597178444 2202 88-89 1.1655341984324785 2202 90-91 1.184299702418869 2202 92-93 1.0011592653453043 2202 94-95 1.5495308048847356 2202 96-97 0.9953374444673244 2202 98-99 1.0396899462161748 2202 100-101 0.909320099363164 2305 1 5.850884554135778 2305 2 7.18051207016299 2305 3 6.733779023338897 2305 4 7.954511548439509 2305 5 7.770654195490788 2305 6 6.301589102529178 2305 7 3.1699308580774606 2305 8 6.120595110568733 2305 9 6.470229775820087 2305 10-11 4.990648892330519 2305 12-13 5.53551027896847 2305 14-15 5.76502988302871 2305 16-17 5.152692834710635 2305 18-19 4.491634198954134 2305 20-21 5.147249129496952 2305 22-23 5.1554167740129095 2305 24-25 5.315791108962344 2305 26-27 6.25227990435015 2305 28-29 5.297177521168049 2305 30-31 5.428006085046622 2305 32-33 5.618678912475598 2305 34-35 5.4124537235381425 2305 36-37 4.823464108172612 2305 38-39 4.650178270684403 2305 40-41 4.1850938934887765 2305 42-43 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1209 48-49 -20.513849240840674 1209 50-51 -22.735883337645987 1209 52-53 -25.352278076797848 1209 54-55 -22.437693825720153 1209 56-57 -26.2239855577219 1209 58-59 -26.553200599122626 1209 60-61 -26.312030506896246 1209 62-63 -26.467102765271935 1209 64-65 -26.346911436679804 1209 66-67 -14.121305776858058 1209 68-69 -26.01347493623037 1209 70-71 -25.852801590555252 1209 72-73 -16.112229194073354 1209 74-75 -16.7167899258283 1209 76-77 -13.727122635963449 1209 78-79 -14.590489365551377 1209 80-81 -24.801519030395525 1209 82-83 -16.378456950139302 1209 84-85 -13.698723258436235 1209 86-87 -13.537866479968397 1209 88-89 -10.752414215021213 1209 90-91 -13.606634179239094 1209 92-93 -17.859533634360417 1209 94-95 -23.256976537470578 1209 96-97 -18.91743165457652 1209 98-99 -10.120642681648064 1209 100-101 -5.504202458024658 1212 1 -20.951618166212427 1212 2 -20.7512071899023 1212 3 -21.667472336835203 1212 4 -23.73455265177812 1212 5 -23.91623372616318 1212 6 -24.997921234794 1212 7 -24.580341176742998 1212 8 -24.193061037418207 1212 9 -25.134775664876322 1212 10-11 -24.866668844339774 1212 12-13 -25.149609416352536 1212 14-15 -25.546586003804798 1212 16-17 -26.62029410761798 1212 18-19 -21.33270572171701 1212 20-21 -27.058001142537865 1212 22-23 -27.303778002918538 1212 24-25 -23.37991232043146 1212 26-27 -26.034988866052462 1212 28-29 -21.02479288846374 1212 30-31 -15.39882971122562 1212 32-33 -26.294814014619067 1212 34-35 -22.418636872832383 1212 36-37 -26.715436871152743 1212 38-39 -27.025556223330828 1212 40-41 -21.449888204864973 1212 42-43 -17.89601393974292 1212 44-45 -15.158628372288923 1212 46-47 -18.092174656334596 1212 48-49 -20.973718021595186 1212 50-51 -13.130099970900343 1212 52-53 -6.107116786475267 1212 54-55 -4.6983402020034895 1212 56-57 -5.027856525463836 1212 58-59 -3.6661038249290776 1212 60-61 -14.737836958509149 1212 62-63 -12.84065115236871 1212 64-65 -17.140459823776578 1212 66-67 -13.378384933652907 1212 68-69 -8.672829774940048 1212 70-71 -15.968930622813316 1212 72-73 -12.718191164792021 1212 74-75 -9.432951762897737 1212 76-77 -19.917055203851184 1212 78-79 -10.721239623737393 1212 80-81 -8.493777094911653 1212 82-83 -5.886393284505381 1212 84-85 -4.967199049700437 1212 86-87 -3.751795159878487 1212 88-89 -3.520846266536914 1212 90-91 -3.672328851494729 1212 92-93 -3.8791290416868236 1212 94-95 -3.61246040843832 1212 96-97 -13.461363371969147 1212 98-99 -10.783010703031941 1212 100-101 -7.082478382938092 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 337.0 3 844.0 4 357.0 5 2190.0 6 2960.0 7 2107.0 8 4243.0 9 8657.0 10 10704.0 11 12203.0 12 17238.0 13 22590.0 14 26733.0 15 28313.0 16 29816.0 17 32668.0 18 38210.0 19 43277.0 20 47746.0 21 54865.0 22 62233.0 23 69298.0 24 73775.0 25 77483.0 26 82972.0 27 87968.0 28 89794.0 29 90793.0 30 94676.0 31 101168.0 32 110321.0 33 129852.0 34 150014.0 35 167817.0 36 178299.0 37 168960.0 38 63864.0 39 216.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 28.58936383388188 26.345340625028296 22.13741759157808 22.92787794951174 2 26.88580294802172 34.45073700543057 19.798138091543834 18.865321955003882 3 17.32671629782563 29.040080324514296 29.73350520526669 23.899698172393386 4 20.69004816879478 28.34772082915274 29.162780967289347 21.799450034763133 5 20.283698338648783 27.762710322414343 29.111199842305574 22.842391496631304 6 20.80243386751065 29.270765337547438 27.51170678448322 22.41509401045869 7 21.40034579577511 29.60488689448247 28.751530840234928 20.24323646950749 8 20.044125245374488 28.708853617539297 29.71161182867303 21.535409308413183 9 20.540976008657946 27.949149128745688 30.13469802745404 21.37517683514233 10-11 21.55408081996366 28.284316979904396 28.42831724252481 21.733284957607136 12-13 21.462235406515855 28.72156046340159 28.097277213988498 21.718926916094052 14-15 21.679693497216178 28.793298817432543 27.83991014111767 21.68709754423361 16-17 21.92789081965269 28.76806424012297 27.64771190213754 21.656333038086803 18-19 21.923481901902797 28.957333857323004 27.597656328511256 21.521527912262943 20-21 22.06315786488146 28.797221976675537 27.586067528543424 21.553552629899585 22-23 21.66839662457234 28.66701817519784 28.02092027455496 21.64366492567486 24-25 21.662537965173563 28.723626835681003 28.15741170195359 21.456423497191842 26-27 21.610913658029578 28.762064218227156 28.01670745375932 21.610314669983943 28-29 21.67565069142589 28.789306952652037 28.108015172743485 21.427027183178588 30-31 21.74370395117082 28.615549835525044 27.989394697958105 21.651351515346036 32-33 21.654398318563747 28.833579204323044 27.90705037031857 21.60497210679464 34-35 21.75708997919698 28.595379032994856 28.053958403773006 21.593572584035158 36-37 21.751641947136665 28.697025737135746 27.926445874429785 21.624886441297804 38-39 21.607192712229082 28.64016642696993 28.265223024718278 21.48741783608271 40-41 21.813357140856056 28.541012062051134 28.002726504677035 21.642904292415775 42-43 21.594495272682426 28.927106447245748 28.021700656293223 21.456697623778595 44-45 21.607163523881155 28.517000914505243 28.202641187618298 21.6731943739953 46-47 21.724532938125645 28.7173604384169 28.004496678779418 21.55360994467804 48-49 21.576607936592943 28.62199119361868 28.317966330069368 21.483434539719013 50-51 21.921323473000715 28.538688476569313 27.905919221836562 21.634068828593414 52-53 21.61147083468626 28.715802097486225 28.192897922634508 21.479829145193012 54-55 21.626591468047504 28.61624777111685 28.24395490508211 21.51320585575354 56-57 21.685330922965957 28.641792029790054 28.110466465320773 21.562410581923224 58-59 21.726973248183686 28.657800089949433 28.144195354907243 21.471031306959638 60-61 21.832808258626937 28.437931573220705 28.0372482293673 21.692011938785058 62-63 21.850040162510247 28.61879872538996 28.06994561887907 21.46121549322072 64-65 21.983982922038 28.52939179457874 28.031717142083146 21.454908141300113 66-67 22.077829737698153 28.6393682647276 27.832510444971792 21.45029155260246 68-69 21.963738452719156 28.609066655593736 28.03159015385609 21.39560473783102 70-71 22.20285369925433 28.51091785224064 27.837853331712143 21.448375116792885 72-73 22.1565386868086 28.68004485958026 27.89067469339924 21.272741760211897 74-75 22.282873933833372 28.48989147046086 27.809729664366635 21.41750493133913 76-77 22.463784271547112 28.65441161091412 27.586516787820546 21.295287329718214 78-79 22.462537653992882 28.631512010717447 27.739222634096244 21.16672770119343 80-81 22.740908733137346 28.521947293817657 27.49660893927437 21.240535033770627 82-83 22.63893766194283 28.64339312353983 27.63814311152383 21.079526102993512 84-85 22.936768822187766 28.441404703850022 27.460567935279656 21.16125853868256 86-87 22.970058728357586 28.608416166385947 27.326537225351323 21.094987879905148 88-89 23.081340278202312 28.565112496940497 27.42199787019744 20.93154935465975 90-91 23.486799683456187 28.466319592010198 27.024217982912496 21.022662741621115 92-93 23.52002090094054 28.594342995434797 27.03933135324423 20.846304750380433 94-95 23.848030922678593 28.442138585677295 26.88501732229022 20.824813169353888 96-97 24.14648737342132 28.54540297921348 26.643786147908887 20.664323499456312 98-99 24.21312350570613 28.571722900800406 26.618530297022815 20.59662329647065 100-101 24.822636814266193 28.411175778216162 26.165251881062158 20.600935526455487 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 155.0 1 158.5 2 162.5 3 171.0 4 200.5 5 236.5 6 283.0 7 332.0 8 404.5 9 552.0 10 750.0 11 1005.0 12 1339.5 13 1826.0 14 2382.0 15 2983.5 16 3818.0 17 4793.0 18 5998.5 19 7621.5 20 9615.5 21 12009.0 22 14915.0 23 18339.0 24 22161.5 25 26410.5 26 30953.5 27 35638.0 28 40338.0 29 45059.0 30 49654.0 31 54247.5 32 58582.5 33 62874.0 34 66886.0 35 69859.0 36 72795.0 37 75239.0 38 76138.0 39 75669.5 40 74018.0 41 71647.5 42 68528.5 43 64637.5 44 60805.0 45 57524.5 46 54509.0 47 51848.5 48 49279.5 49 46755.5 50 44408.0 51 41979.5 52 39534.0 53 37876.5 54 36979.0 55 35906.5 56 35045.5 57 34288.5 58 33472.0 59 32537.0 60 31018.5 61 28938.0 62 26758.0 63 24595.5 64 22212.0 65 19822.0 66 17626.5 67 15468.0 68 13293.0 69 11248.5 70 9505.5 71 7963.5 72 6528.0 73 5311.0 74 4403.0 75 3558.5 76 2782.0 77 2236.0 78 1753.0 79 1344.5 80 1086.0 81 867.0 82 659.5 83 530.0 84 458.5 85 352.0 86 261.0 87 208.0 88 164.0 89 136.5 90 116.5 91 94.5 92 70.0 93 49.0 94 35.0 95 24.5 96 13.5 97 6.5 98 3.0 99 2.0 100 0.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 14.100452927188947 2 11.53301143276257 3 10.090406993902253 4 12.078592178392642 5 9.473585958021761 6 5.582731390247172 7 9.997616172689758 8 10.471361815112916 9 5.720544976781705 10-11 6.615807108563889 12-13 5.581839170812437 14-15 7.613377068862411 16-17 2.7811166103348293 18-19 3.2493030393569433 20-21 3.2074144807671803 22-23 3.242462690357304 24-25 3.6685089091542173 26-27 4.516369023788401 28-29 1.8001785353966329 30-31 2.3493052813442405 32-33 3.169666735451447 34-35 2.0922545744547967 36-37 3.2241378758131205 38-39 1.423089998403156 40-41 1.1905181324154301 42-43 0.5695105284181041 44-45 1.3612294509281597 46-47 1.4221520241256134 48-49 1.319089240702959 50-51 1.5948308008790422 52-53 2.5584506678148085 54-55 1.9011137186287639 56-57 1.8983455506389433 58-59 1.6226497453056674 60-61 1.45228158811399 62-63 0.6295637596022258 64-65 1.2777726176482835 66-67 0.5776777678591446 68-69 0.828780345183685 70-71 1.0314971762398761 72-73 0.08501249793531272 74-75 0.1066316611615965 76-77 0.46484632549720645 78-79 0.9205874372758299 80-81 0.3052076789437586 82-83 0.5204842143504574 84-85 0.06664192854832238 86-87 0.10082079612511387 88-89 0.08192404604584361 90-91 0.09045732422933975 92-93 0.17574435122149418 94-95 0.4726017713529844 96-97 0.3801541114615424 98-99 0.06492612194306176 100-101 0.23232021435228759 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2185561.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.51273503174703 #Duplication Level Percentage of deduplicated Percentage of total 1 98.87371730578546 97.40320314548704 2 0.8833985090503568 1.7405200649903636 3 0.1867662779004381 0.5519657054301447 4 0.03189346822049625 0.12567651136196767 5 0.007668443143114264 0.03777196537318164 6 0.00853607883530151 0.050454748350729696 7 0.003665007523167399 0.025273494051340457 8 0.0016063703507207582 0.012659834937872686 9 0.0014304919640507712 0.012682950823761932 >10 0.0013180472268931518 0.039791579193601184 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 4.5754842806949796E-5 0.0 0.0 5 4.5754842806949796E-5 0.0 0.0 6 4.5754842806949796E-5 0.0 0.0 7 4.5754842806949796E-5 0.0 0.0 8 4.5754842806949796E-5 4.5754842806949796E-5 0.0 9 9.150968561389959E-5 4.5754842806949796E-5 4.5754842806949796E-5 10-11 1.6014194982432426E-4 4.5754842806949796E-5 4.5754842806949796E-5 12-13 2.2877421403474897E-4 4.5754842806949796E-5 4.5754842806949796E-5 14-15 3.4316132105212345E-4 4.5754842806949796E-5 4.5754842806949796E-5 16-17 4.8042584947297285E-4 4.5754842806949796E-5 9.150968561389959E-5 18-19 5.719355350868724E-4 6.86322642104247E-5 9.150968561389959E-5 20-21 6.176903778938223E-4 1.3726452842084937E-4 1.1438710701737448E-4 22-23 6.405677992972972E-4 1.3726452842084937E-4 1.3726452842084937E-4 24-25 7.549549063146716E-4 1.6014194982432426E-4 1.8301937122779918E-4 26-27 0.0010294839631563703 1.8301937122779918E-4 1.8301937122779918E-4 28-29 0.001326890441401544 1.8301937122779918E-4 2.2877421403474897E-4 30-31 0.002219109876137065 1.8301937122779918E-4 2.2877421403474897E-4 32-33 0.004140813274028956 1.8301937122779918E-4 2.2877421403474897E-4 34-35 0.0071835103206911175 1.8301937122779918E-4 2.2877421403474897E-4 36-37 0.011873381708403472 1.8301937122779918E-4 2.7452905684169875E-4 38-39 0.019560195299971037 1.8301937122779918E-4 2.7452905684169875E-4 40-41 0.02990078977434169 1.8301937122779918E-4 3.202838996486486E-4 42-43 0.046235268656422766 1.8301937122779918E-4 3.202838996486486E-4 44-45 0.07007354175884362 1.8301937122779918E-4 3.431613210521235E-4 46-47 0.10136985423879727 1.8301937122779918E-4 4.1179358526254815E-4 48-49 0.14376171609943625 1.8301937122779918E-4 5.033032708764477E-4 50-51 0.1998342759593532 1.8301937122779918E-4 5.490581136833975E-4 52-53 0.2681462562701293 1.8301937122779918E-4 5.490581136833975E-4 54-55 0.3518318637640404 2.2877421403474897E-4 5.490581136833975E-4 56-57 0.4504564274344207 2.5165163543822383E-4 5.490581136833975E-4 58-59 0.5740173804345887 2.9740647824517367E-4 5.948129564903473E-4 60-61 0.7143474833235036 3.202838996486486E-4 5.948129564903473E-4 62-63 0.8795453432779958 3.202838996486486E-4 5.948129564903473E-4 64-65 1.0771376319398085 3.202838996486486E-4 5.948129564903473E-4 66-67 1.298270787225797 3.202838996486486E-4 6.863226421042469E-4 68-69 1.5515467195836674 3.6603874245559837E-4 6.863226421042469E-4 70-71 1.8254123312046655 3.6603874245559837E-4 6.863226421042469E-4 72-73 2.115109118436868 3.6603874245559837E-4 6.863226421042469E-4 74-75 2.4374291085904263 3.6603874245559837E-4 7.092000635077218E-4 76-77 2.789535501411308 3.6603874245559837E-4 7.320774849111967E-4 78-79 3.177948361999505 4.1179358526254815E-4 7.320774849111967E-4 80-81 3.6132141816220185 4.1179358526254815E-4 7.320774849111967E-4 82-83 4.0773741844771205 4.1179358526254815E-4 7.320774849111967E-4 84-85 4.570268228614987 4.1179358526254815E-4 7.778323277181465E-4 86-87 5.1133324578906745 4.8042584947297285E-4 7.778323277181465E-4 88-89 5.678999579512995 5.033032708764478E-4 8.235871705250963E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTCTAA 750 4.3625676E-4 8.007417 2 AGGCGGA 975 1.9237728E-4 7.004235 5 TACATCT 1040 0.009103377 6.002939 1 CGTGTAG 5945 0.0 5.9405084 94-95 GTAGGGG 2100 0.0 5.929175 92-93 CGGGAGG 1350 5.9985067E-4 5.88571 1 GTAGGGA 3825 0.0 5.3859315 94-95 TCGTGTA 6225 0.0 5.273264 94-95 GTCGTGT 7325 0.0 5.192213 92-93 GTGTAGG 5790 0.0 5.122048 94-95 TGTAGGG 5560 0.0 5.0896287 94-95 >>END_MODULE