FastQCFastQC Report
Thu 11 Sep 2014
NYCLib2_TGACCA_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNYCLib2_TGACCA_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2185561
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCTAA7504.3625676E-48.0074172
AGGCGGA9751.9237728E-47.0042355
TACATCT10400.0091033776.0029391
CGTGTAG59450.05.940508494-95
GTAGGGG21000.05.92917592-93
CGGGAGG13505.9985067E-45.885711
GTAGGGA38250.05.385931594-95
TCGTGTA62250.05.27326494-95
GTCGTGT73250.05.19221392-93
GTGTAGG57900.05.12204894-95
TGTAGGG55600.05.089628794-95
Produced by FastQC (version 0.11.2)