##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	NYCLib2_TGACCA_L007_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2185561
Sequences flagged as poor quality	0
Sequence length	101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.268495365720746	33.0	33.0	33.0	27.0	33.0
2	31.662171405877025	33.0	33.0	33.0	27.0	33.0
3	31.809144196844656	33.0	33.0	33.0	27.0	33.0
4	35.65989555999581	37.0	37.0	37.0	33.0	37.0
5	35.17512849103731	37.0	37.0	37.0	33.0	37.0
6	34.977836354144316	37.0	33.0	37.0	33.0	37.0
7	34.774688512468884	37.0	33.0	37.0	27.0	37.0
8	34.82061447838793	37.0	33.0	37.0	27.0	37.0
9	34.72808491732786	37.0	33.0	37.0	27.0	37.0
10-11	34.57911607134278	37.0	33.0	37.0	27.0	37.0
12-13	34.57332213559813	37.0	33.0	37.0	27.0	37.0
14-15	35.502713948501096	37.0	33.0	40.0	27.0	40.0
16-17	35.40793668078814	37.0	33.0	40.0	27.0	40.0
18-19	35.627018875245305	37.0	33.0	40.0	27.0	40.0
20-21	35.3223858771272	37.0	33.0	40.0	27.0	40.0
22-23	35.365036253849695	37.0	33.0	40.0	27.0	40.0
24-25	35.21931325641334	37.0	33.0	40.0	27.0	40.0
26-27	34.862240404179985	37.0	33.0	40.0	27.0	40.0
28-29	34.523844678780414	37.0	33.0	40.0	27.0	40.0
30-31	34.43204765275369	37.0	33.0	40.0	27.0	40.0
32-33	34.44673632994	37.0	33.0	40.0	27.0	40.0
34-35	34.32618856211289	37.0	33.0	40.0	24.5	40.0
36-37	34.05320876424863	37.0	33.0	38.5	22.0	40.0
38-39	33.99702936683076	37.0	33.0	37.0	22.0	40.0
40-41	33.74215498903943	37.0	33.0	37.0	22.0	40.0
42-43	33.39065233136938	37.0	33.0	37.0	22.0	40.0
44-45	33.32329960133806	37.0	33.0	37.0	22.0	40.0
46-47	33.19231629773775	37.0	33.0	37.0	22.0	40.0
48-49	33.23743606332653	37.0	33.0	37.0	22.0	40.0
50-51	33.5240807737693	37.0	33.0	37.0	22.0	40.0
52-53	33.37812991721576	37.0	33.0	37.0	22.0	40.0
54-55	33.03133039983784	37.0	33.0	37.0	22.0	40.0
56-57	33.08545128687783	37.0	33.0	37.0	22.0	40.0
58-59	32.811004359978966	37.0	33.0	37.0	22.0	40.0
60-61	32.724830375359005	37.0	33.0	37.0	22.0	40.0
62-63	32.70864940397454	37.0	33.0	37.0	22.0	40.0
64-65	32.38018591107729	37.0	30.0	37.0	22.0	40.0
66-67	32.03544490407726	33.0	27.0	37.0	22.0	40.0
68-69	31.87884140502141	33.0	27.0	37.0	22.0	40.0
70-71	31.721821308121804	33.0	27.0	37.0	22.0	40.0
72-73	31.500126740914574	33.0	27.0	37.0	22.0	38.5
74-75	31.146115573987643	33.0	27.0	37.0	15.0	37.0
76-77	30.055907384877383	33.0	27.0	37.0	15.0	37.0
78-79	30.957394691797667	33.0	27.0	37.0	22.0	37.0
80-81	30.65358482330166	33.0	27.0	37.0	15.0	37.0
82-83	30.62170010354321	33.0	27.0	37.0	15.0	37.0
84-85	30.499574479961893	33.0	27.0	37.0	15.0	37.0
86-87	30.295821988038767	33.0	27.0	37.0	15.0	37.0
88-89	30.145465626445567	33.0	27.0	37.0	15.0	37.0
90-91	30.06199804077763	33.0	27.0	37.0	15.0	37.0
92-93	29.846606203167056	33.0	27.0	37.0	15.0	37.0
94-95	29.755734568836104	33.0	27.0	33.0	15.0	37.0
96-97	29.612300457411163	33.0	27.0	33.0	15.0	37.0
98-99	29.345103156580848	33.0	27.0	33.0	15.0	37.0
100-101	28.53085500702108	33.0	27.0	33.0	15.0	37.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
2306	1	1.076446529811225
2306	2	0.544924476682116
2306	3	0.4650935262710192
2306	4	0.37493814542270343
2306	5	0.6491363904960252
2306	6	0.6895666481528266
2306	7	0.856493248733976
2306	8	0.7859068446191486
2306	9	0.9675339155444291
2306	10-11	1.188730277248986
2306	12-13	1.3677935890180422
2306	14-15	1.4245302842584024
2306	16-17	1.3660239562517091
2306	18-19	1.2259151909692534
2306	20-21	1.4756640820785947
2306	22-23	1.2358465259725193
2306	24-25	1.2780134632662907
2306	26-27	1.3047695289852683
2306	28-29	1.4969215818550268
2306	30-31	1.4253191417490143
2306	32-33	1.316711931033396
2306	34-35	1.151260025379166
2306	36-37	1.3291188967824539
2306	38-39	1.0284884387251694
2306	40-41	1.2490951621488904
2306	42-43	1.1368609883269798
2306	44-45	1.0276750964328656
2306	46-47	0.9773076125461984
2306	48-49	0.8714967873631991
2306	50-51	1.0129309835837432
2306	52-53	1.1945460341270149
2306	54-55	1.5652933640339697
2306	56-57	1.1269432260588417
2306	58-59	1.0004974509266944
2306	60-61	0.8657324789091732
2306	62-63	0.6159986310402914
2306	64-65	0.6619267195988527
2306	66-67	0.9661561762953319
2306	68-69	0.8677867363357521
2306	70-71	0.779479022516508
2306	72-73	0.4797795434406069
2306	74-75	0.6464574292712904
2306	76-77	0.7989754371377735
2306	78-79	0.7633174752148726
2306	80-81	1.2793327478959604
2306	82-83	1.1854104018235105
2306	84-85	0.916684012388135
2306	86-87	0.6799573546479678
2306	88-89	0.5411360038439845
2306	90-91	0.3338204917134533
2306	92-93	0.1940025227710933
2306	94-95	-0.0012302507240242733
2306	96-97	0.10910409587861025
2306	98-99	0.32242283885389966
2306	100-101	0.2678349767927948
2307	1	0.5498631579587077
2307	2	0.4654742971593784
2307	3	0.40184325430224277
2307	4	0.3789753759635133
2307	5	0.5159731485348615
2307	6	0.6318329767844872
2307	7	0.7371803375708836
2307	8	0.758470074440929
2307	9	0.961116012762183
2307	10-11	1.1109158323220782
2307	12-13	1.3161102073606443
2307	14-15	1.2483098331404108
2307	16-17	1.3679210610098806
2307	18-19	1.0844559909543037
2307	20-21	1.4030302104105061
2307	22-23	1.272607249479499
2307	24-25	1.1477833286592514
2307	26-27	1.2252283274669153
2307	28-29	1.4384845502132677
2307	30-31	1.388458828646428
2307	32-33	1.2159432982542668
2307	34-35	1.2317916367883157
2307	36-37	1.236571985087103
2307	38-39	1.0157359513197761
2307	40-41	1.1449653688451562
2307	42-43	1.1396477694773282
2307	44-45	0.9629426063194515
2307	46-47	1.007736296030572
2307	48-49	0.9269459677216858
2307	50-51	0.8379997111433966
2307	52-53	1.0986517354329308
2307	54-55	1.4702653267380654
2307	56-57	1.0761663758541076
2307	58-59	0.9527844631921312
2307	60-61	0.9469245425568644
2307	62-63	0.7646645133163332
2307	64-65	0.9124986477524075
2307	66-67	0.9636658527808883
2307	68-69	0.7911353606173179
2307	70-71	0.7931153033843863
2307	72-73	0.6451541366546962
2307	74-75	0.7178890822344677
2307	76-77	0.9413242668549735
2307	78-79	0.9303498640111592
2307	80-81	1.3883385794667475
2307	82-83	1.1184873608157524
2307	84-85	0.9972091805885661
2307	86-87	0.9803871120004608
2307	88-89	0.8002198542681604
2307	90-91	0.4847227977285158
2307	92-93	0.5544945177846081
2307	94-95	0.4983096901665398
2307	96-97	0.6368537585439498
2307	98-99	0.6442140742224467
2307	100-101	0.7787654368285573
1101	1	1.0294059276678453
1101	2	0.6347921567841972
1101	3	0.40341969343238304
1101	4	0.3215864112090898
1101	5	0.5739321777115691
1101	6	0.6496568956862774
1101	7	0.7384015772128052
1101	8	0.6356637495725863
1101	9	0.7997258394997928
1101	10-11	0.9704467372524945
1101	12-13	1.1855505771915489
1101	14-15	1.2580997776331557
1101	16-17	1.291345601789729
1101	18-19	1.0288836505659091
1101	20-21	1.2791657973365318
1101	22-23	1.04300368243549
1101	24-25	1.032307803377435
1101	26-27	1.0639180490551752
1101	28-29	1.341333770820043
1101	30-31	1.349003432575394
1101	32-33	0.9183622310879471
1101	34-35	0.9835992488435252
1101	36-37	1.1226798369842186
1101	38-39	0.8967183017819309
1101	40-41	1.0006338548541862
1101	42-43	1.085370054690351
1101	44-45	0.9740132287897509
1101	46-47	0.9306207418979184
1101	48-49	0.7959782801461728
1101	50-51	0.8772544044830752
1101	52-53	1.0316341179388644
1101	54-55	1.438977516160243
1101	56-57	1.089263909942808
1101	58-59	1.1366347455349555
1101	60-61	0.8957638713657303
1101	62-63	0.671161788594361
1101	64-65	0.5446630414570635
1101	66-67	0.8498596077358762
1101	68-69	0.6326975059363917
1101	70-71	0.7342467460779609
1101	72-73	0.6175031511227473
1101	74-75	0.8451858267303045
1101	76-77	0.791745370654187
1101	78-79	0.750625861023078
1101	80-81	1.2822008810850463
1101	82-83	1.183963944686461
1101	84-85	1.0484759252474838
1101	86-87	0.7756237624077755
1101	88-89	0.8624811174393692
1101	90-91	0.630948613621598
1101	92-93	0.7573888857770008
1101	94-95	0.7385105187841425
1101	96-97	0.6949038406704204
1101	98-99	0.8366352072043881
1101	100-101	0.694175744451627
2201	1	1.4018526899451125
2201	2	0.8357917221905424
2201	3	0.3156317751359907
2201	4	0.2959453855680607
2201	5	0.43290653668593393
2201	6	0.5084139578331701
2201	7	0.7546988392727769
2201	8	0.5176715722583793
2201	9	0.6484872475832333
2201	10-11	0.7680347424676199
2201	12-13	1.1338339322545608
2201	14-15	1.059599125742679
2201	16-17	1.0455828899253206
2201	18-19	0.9354460147553922
2201	20-21	1.1377055626559667
2201	22-23	0.8388533130308815
2201	24-25	0.8885659520084701
2201	26-27	0.9516842376688217
2201	28-29	1.3842498942446397
2201	30-31	1.413214645091692
2201	32-33	0.9933296365638355
2201	34-35	1.0165197182046697
2201	36-37	1.012075751803863
2201	38-39	0.7519986146893629
2201	40-41	1.0480045632418467
2201	42-43	1.0384339399576277
2201	44-45	0.9554343500413793
2201	46-47	1.0307945793598847
2201	48-49	0.991219479624661
2201	50-51	0.867910640902025
2201	52-53	1.1876532400596815
2201	54-55	1.503188728676541
2201	56-57	1.1146876387563722
2201	58-59	1.2332231853437392
2201	60-61	0.8291623937473034
2201	62-63	0.7076676556087023
2201	64-65	0.8093305773110444
2201	66-67	0.8701768611039746
2201	68-69	0.7896944637808048
2201	70-71	0.9667543514669212
2201	72-73	0.871523142430874
2201	74-75	0.8859289818802409
2201	76-77	0.8843138191548405
2201	78-79	0.8729639748866127
2201	80-81	1.3435872348442786
2201	82-83	1.1559326539433101
2201	84-85	1.0680326397194513
2201	86-87	1.1666494034334178
2201	88-89	0.9772138423415839
2201	90-91	0.8972458756815698
2201	92-93	0.9769673299317176
2201	94-95	0.9688451558984426
2201	96-97	0.7973592948207937
2201	98-99	0.9263787969479758
2201	100-101	0.9619940843038641
2304	1	-1.6426311995459209
2304	2	-0.15817615278611186
2304	3	-0.14296366704123642
2304	4	0.028069536234177406
2304	5	0.396931257908939
2304	6	0.5780527913070017
2304	7	0.5913897140277768
2304	8	0.6827429835009156
2304	9	1.0161203178914064
2304	10-11	0.8454758410645269
2304	12-13	1.1244529925897808
2304	14-15	1.2505657737340314
2304	16-17	1.1658536295548458
2304	18-19	0.9007225053330856
2304	20-21	1.201425282910293
2304	22-23	0.935858631130742
2304	24-25	1.0561569592359845
2304	26-27	1.0814420346106104
2304	28-29	1.412167387773728
2304	30-31	1.3988685165532004
2304	32-33	0.9108964706101403
2304	34-35	0.8980423319102542
2304	36-37	1.2748776765825056
2304	38-39	0.9865002020906886
2304	40-41	1.127902163202748
2304	42-43	0.8561828146393182
2304	44-45	1.1268251719120883
2304	46-47	0.9045181568510756
2304	48-49	0.8699971718799873
2304	50-51	0.813758264337963
2304	52-53	1.0321319807503855
2304	54-55	1.4099226613651368
2304	56-57	1.0805339008146007
2304	58-59	0.9398144351757409
2304	60-61	0.45063620729789733
2304	62-63	0.2751432948017154
2304	64-65	0.4447087697854144
2304	66-67	0.5798328688812795
2304	68-69	0.5993351996927316
2304	70-71	0.5107090490054418
2304	72-73	0.2730694406179701
2304	74-75	0.43436252483083493
2304	76-77	0.5417117725503466
2304	78-79	0.5935470459802019
2304	80-81	1.3856633184049443
2304	82-83	0.9001015066684204
2304	84-85	0.5146531940794397
2304	86-87	0.48128735550244883
2304	88-89	0.38045697567456926
2304	90-91	0.3258981641051477
2304	92-93	0.3326070116485873
2304	94-95	0.18520385449116006
2304	96-97	0.1832145803697678
2304	98-99	0.4455073755823733
2304	100-101	0.48200869293395243
1102	1	1.4322373648985867
1102	2	0.14245649301361496
1102	3	0.28815644311731603
1102	4	0.2149105035998673
1102	5	0.3141278950734048
1102	6	0.18456880370781903
1102	7	-4.508395042798497
1102	8	-1.025765105270537
1102	9	0.23278738345202044
1102	10-11	0.7082636629918895
1102	12-13	0.9228929981423803
1102	14-15	0.8667375725702229
1102	16-17	0.6116948410436294
1102	18-19	0.8588132712573255
1102	20-21	1.1034365061903841
1102	22-23	0.6637400415559256
1102	24-25	0.9457303501376941
1102	26-27	0.9856159013130181
1102	28-29	0.7527810143100666
1102	30-31	1.190925740283241
1102	32-33	0.9142425525940112
1102	34-35	1.1571348003138624
1102	36-37	1.2989884223067634
1102	38-39	0.9047062004983033
1102	40-41	1.0396486325924315
1102	42-43	1.0693791837470528
1102	44-45	0.9245513168573822
1102	46-47	0.810221542503335
1102	48-49	0.8020232499706594
1102	50-51	0.7543268956370994
1102	52-53	1.067086419083779
1102	54-55	1.4039647813747749
1102	56-57	1.1285726988354483
1102	58-59	1.1292718277177016
1102	60-61	0.9036232049508115
1102	62-63	0.7453210259886802
1102	64-65	0.36993562077299913
1102	66-67	0.4693660512731448
1102	68-69	0.6748086469182653
1102	70-71	0.7874579995154711
1102	72-73	0.5835037084800412
1102	74-75	0.5069808487422911
1102	76-77	0.5744911219702722
1102	78-79	0.6833390307647882
1102	80-81	1.2834668487759302
1102	82-83	1.039350756348906
1102	84-85	0.6651180650851138
1102	86-87	0.6581181590503711
1102	88-89	0.47500712701492986
1102	90-91	0.011733280494425458
1102	92-93	0.14605803465200395
1102	94-95	0.2701580534881849
1102	96-97	0.1514053410286138
1102	98-99	0.3188135522158042
1102	100-101	0.48103267922476434
2202	1	1.5205723392469288
2202	2	0.9636816980432528
2202	3	0.34861246568816284
2202	4	-0.2869250423296208
2202	5	-0.027159962525153958
2202	6	0.43956352374630825
2202	7	0.6101032860430635
2202	8	-3.78155166510021
2202	9	-0.2876420007949889
2202	10-11	0.6219318137218295
2202	12-13	0.06476870403908208
2202	14-15	0.012687068202630769
2202	16-17	0.23819578635750815
2202	18-19	0.7996327446202045
2202	20-21	1.1848041963951204
2202	22-23	-2.761746608909945
2202	24-25	-2.897963158821426
2202	26-27	-1.321143410346103
2202	28-29	0.5437682898945511
2202	30-31	1.1666872335943168
2202	32-33	1.0974507306486672
2202	34-35	-2.2093024903194447
2202	36-37	-0.34384055944828873
2202	38-39	0.3116513489566799
2202	40-41	1.1363565585748177
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1209	90-91	-5.534885081873114
1209	92-93	-3.2811088688224466
1209	94-95	-1.1458151367279505
1209	96-97	-0.11412846412459743
1209	98-99	1.1706928632959404
1209	100-101	0.6163834802186834
1212	1	-28.65334070423133
1212	2	-16.307841480764694
1212	3	-6.190774696103723
1212	4	-1.6705045450371472
1212	5	-0.5395458332514025
1212	6	-2.5000695906941175
1212	7	-0.46581285970803066
1212	8	-1.1732832720589599
1212	9	0.21943508232548936
1212	10-11	0.46991078514191287
1212	12-13	-0.019443885244143644
1212	14-15	-0.3468492627560167
1212	16-17	-0.17394859007636398
1212	18-19	-0.9794588653107823
1212	20-21	-1.2556399673380128
1212	22-23	-1.1388652104722254
1212	24-25	-0.13778612492863118
1212	26-27	-0.6677802307951026
1212	28-29	0.19932720985777053
1212	30-31	-1.157409066898893
1212	32-33	-0.3396195720551489
1212	34-35	-2.060984312300995
1212	36-37	-1.6584686117968133
1212	38-39	-0.4611786855026949
1212	40-41	-0.2389933759430818
1212	42-43	0.07443820177928018
1212	44-45	0.8190409166113142
1212	46-47	-2.828721900151127
1212	48-49	-1.4896219497678942
1212	50-51	-0.3664066965809809
1212	52-53	-1.0431169720500577
1212	54-55	-0.7047752701458521
1212	56-57	-1.882600356645348
1212	58-59	-0.5732019313656878
1212	60-61	-0.21385228430290937
1212	62-63	-4.701633489755864
1212	64-65	-4.166614804079938
1212	66-67	-1.7926255670128342
1212	68-69	-0.06568272145422327
1212	70-71	-0.9092546768657819
1212	72-73	-1.2130859335669406
1212	74-75	-0.3621767505502582
1212	76-77	0.9615217652091523
1212	78-79	-1.6869158809965228
1212	80-81	0.45986809449856736
1212	82-83	0.5810109508969461
1212	84-85	-3.6612020548733604
1212	86-87	-1.896422192366444
1212	88-89	-0.9704494457098924
1212	90-91	0.17380333316850027
1212	92-93	-2.2998306961719415
1212	94-95	-1.9401629892277974
1212	96-97	-0.24971196451946653
1212	98-99	-0.743181141928531
1212	100-101	-0.15487950501914938
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	9.0
11	15.0
12	33.0
13	156.0
14	513.0
15	1236.0
16	2267.0
17	3851.0
18	5859.0
19	8661.0
20	12098.0
21	16750.0
22	22465.0
23	29266.0
24	37332.0
25	47587.0
26	58962.0
27	72055.0
28	86993.0
29	104713.0
30	123381.0
31	145424.0
32	168820.0
33	194091.0
34	219370.0
35	241426.0
36	256196.0
37	238768.0
38	87040.0
39	224.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	26.770702087006693	26.248051755332728	24.537191080518845	22.444055077141734
2	26.42134913204978	33.76879552174474	20.985415654309737	18.824439691895744
3	17.024203942892257	28.03029713761231	31.62151638509796	23.323982534397473
4	20.42195135342887	27.70269404637713	30.649536045681657	21.225818554512344
5	19.678883362212265	27.796295779436036	30.038328831819385	22.486492026532318
6	20.106508123086016	29.55685062096185	28.24327483881713	22.093366417135005
7	20.774117034482224	29.71960059682617	29.539509535538016	19.96677283315359
8	19.494033797272188	28.658820321189843	30.421479885484782	21.425665996053187
9	20.04963485347698	27.839030802617724	30.71508871177698	21.39624563212832
10-11	20.90518178170273	28.27905512589216	29.08569012715728	21.730072965247825
12-13	21.18776336054415	28.553984857205663	28.555497715469414	21.70275406678077
14-15	21.25811176169414	28.689681962663133	28.31392031611106	21.738285959531673
16-17	21.456774470735002	28.646849117695304	28.199571321602825	21.696805089966873
18-19	21.657531632855704	28.78927150233646	28.036304057475924	21.51689280733191
20-21	21.543077851196525	28.635240250727445	28.1637822425872	21.657899655488833
22-23	21.32201327760087	28.44487718367897	28.525840396548162	21.707269142172
24-25	21.413129169124083	28.42496731960352	28.582730017601886	21.579173493670503
26-27	21.500909835049217	28.603342574286415	28.327555259267527	21.568192331396833
28-29	21.552109504150195	28.542740284988614	28.4465178505656	21.458632360295596
30-31	21.63977578296831	28.381179935037277	28.337255285942604	21.641788996051815
32-33	21.5942725917968	28.49501798394097	28.219345056029095	21.691364368233145
34-35	21.724490874425374	28.343798228463996	28.31991420051877	21.61179669659186
36-37	21.70193373692155	28.44207963081332	28.242954554917475	21.613032077347647
38-39	21.520332765820765	28.332176498391032	28.575592262124005	21.5718984736642
40-41	21.683234821218587	28.25119719901644	28.331498528037745	21.734069451727233
42-43	21.3875750894164	28.583873888672063	28.53882824592862	21.489722775982916
44-45	21.507795938891665	28.138381861682195	28.651041997912664	21.702780201513477
46-47	21.5655871420886	28.349367889225753	28.474103561042103	21.610941407643544
48-49	21.546999749949613	28.23146658784627	28.66856290117016	21.55297076103396
50-51	21.725776980321523	28.288143933787236	28.28800666922741	21.698072416663827
52-53	21.492964951268714	28.400426069876012	28.540367512721016	21.56624146613426
54-55	21.457660675479083	28.138395724029415	28.755206723539594	21.64873687695191
56-57	21.63045736190789	28.383955591631153	28.348403639568176	21.637183406892778
58-59	21.501260435693048	28.2537973373296	28.701819937234703	21.543122289742655
60-61	21.71737068450767	28.17119166223982	28.417127768268248	21.69430988498426
62-63	21.670190976166243	28.324650102755182	28.371229097504525	21.63392982357405
64-65	21.750072065559067	28.237276425182223	28.427460867257526	21.58519064200118
66-67	21.813487173691325	28.33101270253662	28.259220332111777	21.59627979166028
68-69	21.714730078996972	28.332456707696103	28.3769552774503	21.575857935856625
70-71	21.92268816973059	28.27015214457922	28.154479985467766	21.65267970022242
72-73	21.764159430874084	28.448354189519904	28.26526130672933	21.522225072876687
74-75	21.767228948753747	28.234647867981966	28.310315625643725	21.687807557620562
76-77	21.72934744723157	28.508986713738455	28.12159774290068	21.640068096129294
78-79	21.86310943302299	28.518075034453478	28.07505275545445	21.543762777069084
80-81	21.859424880610078	28.378896850634845	27.96050243063199	21.801175838123093
82-83	21.794106248229784	28.561904618097643	27.928744748834	21.715244384838574
84-85	21.8047013593978	28.31234531239061	28.048944543762783	21.83400878444881
86-87	21.83195278726931	28.515931093573226	27.767656442503814	21.884459676653652
88-89	21.82373474346881	28.563456897117778	27.757157502929076	21.855650856484335
90-91	21.987046562172065	28.50705954123851	27.502669266751393	22.00322462983803
92-93	21.844251802292685	28.55954092300906	27.582576910748273	22.013630363949982
94-95	21.94251937570343	28.459981361617793	27.46122857278178	22.136270689896996
96-97	22.004517356266664	28.611516970515922	27.248965725840584	22.134999947376826
98-99	21.867697727771407	28.558429503393434	27.33947842457048	22.234394344264675
100-101	21.993682796775275	28.417209974362816	27.130980722348507	22.458126506513402
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	1.5
2	2.5
3	5.5
4	8.0
5	14.5
6	23.5
7	35.0
8	52.5
9	80.0
10	121.0
11	186.0
12	285.5
13	409.5
14	606.0
15	934.0
16	1389.5
17	2063.5
18	3037.5
19	4312.5
20	5991.5
21	8208.5
22	10845.0
23	14045.5
24	17867.0
25	22233.5
26	27105.0
27	31903.5
28	37029.0
29	42244.0
30	47374.5
31	52289.5
32	56948.0
33	61783.5
34	66118.0
35	70373.0
36	74194.0
37	76942.5
38	78614.5
39	78619.0
40	77459.0
41	75125.5
42	71574.0
43	67547.0
44	63409.0
45	59887.0
46	56941.5
47	54133.0
48	51629.5
49	49051.0
50	46451.5
51	43807.5
52	41216.0
53	39529.0
54	38322.5
55	37634.0
56	37240.5
57	36722.5
58	36118.5
59	34990.5
60	33574.5
61	31906.0
62	29712.0
63	27249.5
64	24624.5
65	21825.0
66	19259.0
67	16832.0
68	14338.0
69	12042.5
70	9882.0
71	7952.5
72	6367.5
73	4979.5
74	3783.5
75	2716.0
76	1865.5
77	1247.5
78	789.5
79	503.5
80	322.0
81	215.0
82	150.5
83	106.0
84	74.5
85	46.0
86	32.0
87	29.5
88	17.0
89	9.0
90	5.0
91	6.0
92	7.0
93	2.5
94	0.0
95	0.0
96	1.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.7164750835140269
2	0.0030655744680656363
3	3.660387424555983E-4
4	4.575484280694979E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.19489275293620265
14-15	0.0
16-17	2.9740647824517367E-4
18-19	0.12191377865911773
20-21	2.2877421403474894E-5
22-23	2.2877421403474894E-5
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.001967458240698841
42-43	0.0
44-45	0.0
46-47	0.024730492537156368
48-49	6.86322642104247E-5
50-51	2.2877421403474894E-5
52-53	1.8301937122779916E-4
54-55	0.0020818453477162158
56-57	0.0012353807557876444
58-59	0.1001573509044131
60-61	0.001555664655436293
62-63	0.0012125033343841694
64-65	0.0023334969831544394
66-67	0.004209445538239381
68-69	0.004461097173677605
70-71	0.0033858583677142846
72-73	0.040149874563098445
74-75	0.046258146077826245
76-77	0.16679927945273548
78-79	2.28774214034749E-4
80-81	0.12319491425771233
82-83	0.00430095522385328
84-85	0.0049415230231505776
86-87	0.006108271514727798
88-89	0.006611574785604245
90-91	0.023060440774702696
92-93	0.01004318799612548
94-95	0.013063007621384168
96-97	0.009700026675073355
98-99	0.010958284852264476
100-101	0.011827626865596522
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2185561.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.90090092586345
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.59160206703424	96.52206666086875
2	0.9847824158032065	1.9282217144616438
3	0.28615299296224483	0.840439074409081
4	0.08022679819374569	0.3141710328626055
5	0.018003696780990935	0.08812890674275402
6	0.015030525640729072	0.08829012009700002
7	0.014750697762032762	0.10108746201316877
8	0.004848915736377781	0.03797705752839851
9	0.0014393391063556207	0.01268213357250382
>10	0.0030196578979548266	0.05182732705116523
>50	0.0	0.0
>100	1.4289308211284826E-4	0.015108510392914927
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	1.3726452842084937E-4	4.5754842806949796E-5	0.0
2	1.3726452842084937E-4	4.5754842806949796E-5	0.0
3	1.3726452842084937E-4	4.5754842806949796E-5	0.0
4	1.8301937122779918E-4	4.5754842806949796E-5	4.5754842806949796E-5
5	1.8301937122779918E-4	4.5754842806949796E-5	4.5754842806949796E-5
6	1.8301937122779918E-4	4.5754842806949796E-5	9.150968561389959E-5
7	1.8301937122779918E-4	4.5754842806949796E-5	9.150968561389959E-5
8	2.2877421403474897E-4	4.5754842806949796E-5	9.150968561389959E-5
9	2.7452905684169875E-4	4.5754842806949796E-5	9.150968561389959E-5
10-11	3.8891616385907323E-4	4.5754842806949796E-5	9.150968561389959E-5
12-13	5.033032708764478E-4	6.86322642104247E-5	9.150968561389959E-5
14-15	6.176903778938222E-4	9.150968561389959E-5	9.150968561389959E-5
16-17	7.549549063146716E-4	9.150968561389959E-5	9.150968561389959E-5
18-19	8.235871705250963E-4	9.150968561389959E-5	9.150968561389959E-5
20-21	8.693420133320461E-4	9.150968561389959E-5	1.3726452842084937E-4
22-23	9.150968561389959E-4	9.150968561389959E-5	1.3726452842084937E-4
24-25	9.837291203494206E-4	9.150968561389959E-5	1.3726452842084937E-4
26-27	0.0013497678628050188	9.150968561389959E-5	1.8301937122779918E-4
28-29	0.0017386840266640923	9.150968561389959E-5	2.0589679263127407E-4
30-31	0.002882555096837837	1.3726452842084937E-4	2.2877421403474897E-4
32-33	0.004895768180343628	1.6014194982432426E-4	2.7452905684169875E-4
34-35	0.008281626548057912	1.8301937122779918E-4	2.7452905684169875E-4
36-37	0.013108762464191116	1.8301937122779918E-4	2.7452905684169875E-4
38-39	0.020955718005583004	1.8301937122779918E-4	2.7452905684169875E-4
40-41	0.031387822165567555	1.8301937122779918E-4	2.7452905684169875E-4
42-43	0.04927796570308493	1.8301937122779918E-4	3.6603874245559837E-4
44-45	0.07547261321006368	1.8301937122779918E-4	3.6603874245559837E-4
46-47	0.11004039695071426	1.8301937122779918E-4	4.1179358526254815E-4
48-49	0.15577236233626057	1.8301937122779918E-4	4.5754842806949793E-4
50-51	0.21600861289161	1.8301937122779918E-4	4.5754842806949793E-4
52-53	0.287065883770803	1.8301937122779918E-4	5.033032708764478E-4
54-55	0.37180385264927407	1.8301937122779918E-4	5.033032708764478E-4
56-57	0.47699423626245163	1.8301937122779918E-4	5.033032708764478E-4
58-59	0.6035521314664747	2.0589679263127407E-4	5.261806922799226E-4
60-61	0.7519350866894129	2.5165163543822383E-4	5.490581136833975E-4
62-63	0.9206789469614438	2.7452905684169875E-4	5.490581136833975E-4
64-65	1.1221146424190402	2.7452905684169875E-4	5.490581136833975E-4
66-67	1.347411488400461	2.7452905684169875E-4	5.490581136833975E-4
68-69	1.6068643245372698	2.7452905684169875E-4	5.490581136833975E-4
70-71	1.8902469434621134	2.9740647824517367E-4	5.490581136833975E-4
72-73	2.2002588809006016	3.431613210521235E-4	5.490581136833975E-4
74-75	2.537151788488173	3.6603874245559837E-4	5.490581136833975E-4
76-77	2.915269809444806	3.6603874245559837E-4	6.405677992972972E-4
78-79	3.3314558596168213	3.6603874245559837E-4	6.405677992972972E-4
80-81	3.78907291995053	3.6603874245559837E-4	6.405677992972972E-4
82-83	4.283499751322429	3.6603874245559837E-4	6.863226421042469E-4
84-85	4.809428791966914	3.6603874245559837E-4	6.863226421042469E-4
86-87	5.385642404856236	3.6603874245559837E-4	6.863226421042469E-4
88-89	5.98807354267394	3.6603874245559837E-4	7.092000635077218E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTAT	870	0.0	11.182787	94-95
GACCAAT	2810	0.0	7.9379244	94-95
CGTATGC	830	3.6379788E-12	7.7191787	94-95
TCTCGTA	985	0.0	7.70902	94-95
ACCAATC	2535	0.0	7.207725	94-95
TCGTATG	1010	1.8189894E-12	7.048557	92-93
ATCTCGT	1515	0.0	6.891922	92-93
GTATGCC	1035	1.8189894E-12	6.8780656	94-95
CTGACCA	3490	0.0	6.867462	92-93
GTCCCGA	355	0.008830628	6.6825595	28-29
CCGATGG	715	0.009444138	6.6358285	5
AATCTCG	1610	0.0	6.632421	94-95
CACTGAC	3640	0.0	6.5855174	90-91
CGAACTC	980	6.4968574E-4	6.339127	1
ACGTCTG	9365	0.0	6.1572094	94-95
TATGCCG	670	2.309118E-5	6.0220456	90-91
ACTGACC	3255	0.0	5.9790583	90-91
CACGTCT	9985	0.0	5.9650087	94-95
CTCCAGT	5980	0.0	5.8728104	94-95
CAGTCAC	4740	0.0	5.8572392	94-95
>>END_MODULE