FastQCFastQC Report
Thu 11 Sep 2014
NYCLib2_TGACCA_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNYCLib2_TGACCA_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2185561
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT8700.011.18278794-95
GACCAAT28100.07.937924494-95
CGTATGC8303.6379788E-127.719178794-95
TCTCGTA9850.07.7090294-95
ACCAATC25350.07.20772594-95
TCGTATG10101.8189894E-127.04855792-93
ATCTCGT15150.06.89192292-93
GTATGCC10351.8189894E-126.878065694-95
CTGACCA34900.06.86746292-93
GTCCCGA3550.0088306286.682559528-29
CCGATGG7150.0094441386.63582855
AATCTCG16100.06.63242194-95
CACTGAC36400.06.585517490-91
CGAACTC9806.4968574E-46.3391271
ACGTCTG93650.06.157209494-95
TATGCCG6702.309118E-56.022045690-91
ACTGACC32550.05.979058390-91
CACGTCT99850.05.965008794-95
CTCCAGT59800.05.872810494-95
CAGTCAC47400.05.857239294-95
Produced by FastQC (version 0.11.2)