##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename NYCLib28_CAAAAG_L007_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4664091 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.661020979221888 27.0 22.0 33.0 2.0 33.0 2 24.701585367866965 27.0 15.0 33.0 2.0 33.0 3 25.547474738378817 33.0 22.0 33.0 6.0 33.0 4 27.608340188902833 33.0 15.0 37.0 2.0 37.0 5 27.6486987496599 33.0 15.0 37.0 6.0 37.0 6 28.704436298519905 33.0 22.0 37.0 15.0 37.0 7 28.379500743017235 33.0 22.0 37.0 6.0 37.0 8 28.14997713380807 33.0 22.0 37.0 6.0 37.0 9 28.903397682420863 33.0 27.0 37.0 15.0 37.0 10-11 28.50793294985025 33.0 22.0 37.0 10.5 37.0 12-13 28.800759247621883 33.0 24.5 37.0 10.5 37.0 14-15 29.112443560813887 33.0 22.0 37.0 6.0 40.0 16-17 29.96534608780146 33.0 27.0 37.0 15.0 40.0 18-19 30.268949405146685 33.0 27.0 37.0 15.0 40.0 20-21 30.27281714700678 33.0 27.0 37.0 15.0 40.0 22-23 30.283900442765805 33.0 27.0 37.0 15.0 40.0 24-25 29.868621023903692 33.0 27.0 37.0 10.5 40.0 26-27 29.36000969963922 33.0 27.0 37.0 6.0 40.0 28-29 29.530924139344624 33.0 27.0 37.0 15.0 40.0 30-31 29.432593617920404 33.0 27.0 37.0 10.5 40.0 32-33 29.25858103969241 33.0 27.0 37.0 6.0 40.0 34-35 29.294678534359644 33.0 24.5 37.0 10.5 40.0 36-37 29.522990439080196 33.0 27.0 37.0 6.0 40.0 38-39 29.554203487882205 33.0 27.0 37.0 15.0 40.0 40-41 29.615170780329972 33.0 27.0 37.0 15.0 40.0 42-43 29.52230048684728 33.0 27.0 37.0 15.0 40.0 44-45 29.252943821207605 33.0 24.5 37.0 10.5 40.0 46-47 28.88167726573088 33.0 22.0 37.0 10.5 40.0 48-49 28.715414536294425 33.0 22.0 37.0 10.5 40.0 50-51 27.96225405979429 33.0 22.0 37.0 10.5 38.5 52-53 27.690401838214562 33.0 22.0 37.0 6.0 37.0 54-55 28.610524751768352 33.0 22.0 37.0 6.0 40.0 56-57 28.517930289095986 33.0 22.0 37.0 6.0 40.0 58-59 28.69900233507451 33.0 22.0 37.0 10.5 40.0 60-61 28.462487974612845 33.0 22.0 37.0 10.5 40.0 62-63 28.33488722668576 33.0 22.0 37.0 10.5 40.0 64-65 28.29506885693268 33.0 22.0 37.0 10.5 40.0 66-67 28.182330061741936 33.0 22.0 37.0 10.5 40.0 68-69 27.888476018156595 33.0 22.0 37.0 6.0 38.5 70-71 27.793230127799823 33.0 22.0 37.0 6.0 37.0 72-73 27.569342665055203 33.0 22.0 37.0 6.0 37.0 74-75 27.520686345956804 33.0 22.0 37.0 6.0 37.0 76-77 27.1399753992793 27.0 22.0 35.0 6.0 37.0 78-79 26.597073792085105 27.0 22.0 33.0 6.0 37.0 80-81 26.476492418351185 27.0 22.0 33.0 6.0 37.0 82-83 26.066502990614893 27.0 22.0 33.0 6.0 37.0 84-85 26.173024175557465 27.0 22.0 33.0 6.0 37.0 86-87 25.881638887405927 27.0 22.0 33.0 6.0 37.0 88-89 25.53493295906962 27.0 22.0 33.0 6.0 37.0 90-91 25.419111248043833 27.0 22.0 33.0 6.0 37.0 92-93 25.322565104325797 27.0 22.0 33.0 6.0 37.0 94-95 25.121597112920824 27.0 18.5 33.0 6.0 37.0 96-97 25.012044683519257 27.0 18.5 33.0 6.0 37.0 98-99 24.79433355824318 27.0 15.0 33.0 6.0 37.0 100-101 24.335289984693695 27.0 15.0 33.0 6.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 8.238431145656126 2306 2 8.422036731275593 2306 3 7.468923626307149 2306 4 8.957584637086317 2306 5 8.801168940017341 2306 6 7.848996651304645 2306 7 8.023224141175376 2306 8 8.396944998197494 2306 9 7.346492434342373 2306 10-11 7.659811887747459 2306 12-13 7.361620620857266 2306 14-15 7.935220859156754 2306 16-17 6.735613015804265 2306 18-19 6.127340973627632 2306 20-21 5.9906375157369744 2306 22-23 5.698467030963279 2306 24-25 5.638457883566041 2306 26-27 6.317350968867814 2306 28-29 5.389214505736575 2306 30-31 5.303130053487688 2306 32-33 5.311984432034947 2306 34-35 4.661973462683562 2306 36-37 4.7017046182906626 2306 38-39 4.383858622575655 2306 40-41 3.588570109778349 2306 42-43 3.3741339331705937 2306 44-45 3.0484648432626855 2306 46-47 3.4109444914614215 2306 48-49 3.220655235725978 2306 50-51 3.0419303608267967 2306 52-53 3.6362894756420907 2306 54-55 3.2039385719872975 2306 56-57 3.442267932788667 2306 58-59 3.094376856679837 2306 60-61 3.034092016710485 2306 62-63 2.609922670433395 2306 64-65 2.1818050011315187 2306 66-67 2.071919024834756 2306 68-69 2.265089860146663 2306 70-71 2.2908363536553473 2306 72-73 1.5599307368727935 2306 74-75 1.1854506509757528 2306 76-77 1.4976689510420442 2306 78-79 1.8338324338156617 2306 80-81 1.5552865053095637 2306 82-83 1.5839426994549903 2306 84-85 1.169997454445145 2306 86-87 1.1519651768214132 2306 88-89 1.1185903876368677 2306 90-91 1.059679053230255 2306 92-93 0.9283912194923154 2306 94-95 1.1694201740726875 2306 96-97 0.8402444912690044 2306 98-99 0.7683366397225555 2306 100-101 0.6045869155011943 2307 1 8.170661675735616 2307 2 8.241264714430695 2307 3 7.461493248704027 2307 4 9.014064756011653 2307 5 8.827563273446756 2307 6 7.416131348701228 2307 7 7.952560134270286 2307 8 8.375671426869285 2307 9 7.47384701419729 2307 10-11 7.617395511505002 2307 12-13 7.152449831930433 2307 14-15 8.065654072110934 2307 16-17 6.689061936123867 2307 18-19 6.275222206932263 2307 20-21 6.219673009171661 2307 22-23 5.8459115512171635 2307 24-25 5.989358827130495 2307 26-27 6.718073955128375 2307 28-29 5.802472427189805 2307 30-31 5.70317706898302 2307 32-33 5.8558450463408676 2307 34-35 5.538005674485394 2307 36-37 5.4493513003024106 2307 38-39 4.930246809398962 2307 40-41 4.37585623312323 2307 42-43 4.162750210139706 2307 44-45 4.201539890516912 2307 46-47 4.527765612556379 2307 48-49 4.25107451455408 2307 50-51 4.106977005070242 2307 52-53 4.627571935578555 2307 54-55 4.261375293028237 2307 56-57 4.595179824093911 2307 58-59 4.205480952007189 2307 60-61 4.165021966643124 2307 62-63 3.8375090147559803 2307 64-65 3.6873948658184865 2307 66-67 3.3546426670711007 2307 68-69 3.472388565130423 2307 70-71 3.277846425397584 2307 72-73 2.6435066409296155 2307 74-75 2.2909528378540145 2307 76-77 2.4210308873359985 2307 78-79 2.84307080812604 2307 80-81 2.541629268830686 2307 82-83 2.7139862245979067 2307 84-85 2.0756199035500202 2307 86-87 1.9916087301833691 2307 88-89 2.1761032494198886 2307 90-91 1.9803773400343267 2307 92-93 1.8627803503283005 2307 94-95 1.9844652397647842 2307 96-97 1.816390797254229 2307 98-99 1.581439697493444 2307 100-101 1.2482676334185534 1101 1 7.941202338846473 1101 2 7.793512362914658 1101 3 7.189707063320771 1101 4 8.661119238897417 1101 5 8.525208710131267 1101 6 7.252283003813247 1101 7 7.066646152819587 1101 8 7.957644299734337 1101 9 7.336755869138344 1101 10-11 7.467950576254758 1101 12-13 7.253477334782929 1101 14-15 7.659997312118332 1101 16-17 6.8567802337604284 1101 18-19 5.971980181486153 1101 20-21 5.7951628835858 1101 22-23 5.323946650895159 1101 24-25 5.8540810924708 1101 26-27 6.450493313213606 1101 28-29 5.866524015301735 1101 30-31 5.5774666660968855 1101 32-33 5.706934555193719 1101 34-35 5.470324624467505 1101 36-37 5.33611484799323 1101 38-39 4.898493914775184 1101 40-41 4.408549552085738 1101 42-43 4.2700373077654845 1101 44-45 4.211419763345184 1101 46-47 4.523604027520541 1101 48-49 4.201059632313658 1101 50-51 3.7046343947668845 1101 52-53 4.708290012282479 1101 54-55 4.411770266648578 1101 56-57 4.5560212841349355 1101 58-59 4.139624256977829 1101 60-61 4.351378559689721 1101 62-63 3.8769001622519497 1101 64-65 3.6310669563995077 1101 66-67 3.3012798509750567 1101 68-69 3.47836963311185 1101 70-71 3.458786897356287 1101 72-73 2.993878688936782 1101 74-75 2.37403478577642 1101 76-77 2.6572964882277645 1101 78-79 3.1989391408353143 1101 80-81 2.8059637095483687 1101 82-83 2.9390203258972605 1101 84-85 2.261718308333112 1101 86-87 2.368963222469077 1101 88-89 2.4342224439605467 1101 90-91 2.2625424875239695 1101 92-93 1.9520697811724403 1101 94-95 2.1084271594769604 1101 96-97 2.0569691265592738 1101 98-99 1.9835082728884785 1101 100-101 1.6176094758399167 2201 1 6.947250797347873 2201 2 7.682971052763641 2201 3 6.196355711928039 2201 4 8.250692958468019 2201 5 3.831357237922873 2201 6 5.652918018322207 2201 7 6.571621614367835 2201 8 7.54490561930724 2201 9 6.484865248144253 2201 10-11 4.071908057604681 2201 12-13 6.298046869421647 2201 14-15 6.908803650602902 2201 16-17 5.83006316162766 2201 18-19 1.8516441402999604 2201 20-21 4.859442847047372 2201 22-23 4.966004317818097 2201 24-25 5.285302140883168 2201 26-27 5.955042495138294 2201 28-29 4.8638447865734165 2201 30-31 4.989150924197485 2201 32-33 5.181358654131934 2201 34-35 4.7961236869766815 2201 36-37 4.800235690162765 2201 38-39 4.199041356567609 2201 40-41 3.966789659394699 2201 42-43 3.835460426680605 2201 44-45 3.64342679849511 2201 46-47 4.1718556978367936 2201 48-49 3.89917532105175 2201 50-51 3.6349620177388715 2201 52-53 4.414078079499095 2201 54-55 3.9931721980793533 2201 56-57 4.125700077669016 2201 58-59 3.8281873496459156 2201 60-61 3.7628178422369096 2201 62-63 3.6812314488978046 2201 64-65 3.427824227489495 2201 66-67 3.0298251095840243 2201 68-69 3.3206034326226472 2201 70-71 3.157529026458967 2201 72-73 2.523691135975426 2201 74-75 2.1152371717254894 2201 76-77 2.402371139894086 2201 78-79 2.8047879905965196 2201 80-81 2.33731559424643 2201 82-83 2.5957498224809576 2201 84-85 2.225666327905209 2201 86-87 2.079976602123203 2201 88-89 2.1213308431851168 2201 90-91 1.8633383639407342 2201 92-93 1.844290217830892 2201 94-95 1.95352141120215 2201 96-97 1.9011084470575277 2201 98-99 1.7507207065421078 2201 100-101 1.4042672872581399 2304 1 1.6804833919430173 2304 2 4.735620263414631 2304 3 5.521450055602351 2304 4 4.115009395921003 2304 5 6.379317340121972 2304 6 6.212071597155443 2304 7 6.990693189799565 2304 8 6.233385652785902 2304 9 5.612191295300217 2304 10-11 6.428437847642805 2304 12-13 6.337363168167933 2304 14-15 6.643782061675527 2304 16-17 5.329625606202779 2304 18-19 5.126764925581263 2304 20-21 4.914023445760986 2304 22-23 4.397155602956232 2304 24-25 4.7044640169457494 2304 26-27 5.579423606388573 2304 28-29 4.906293056936825 2304 30-31 4.816292103576384 2304 32-33 4.8317345698401795 2304 34-35 4.558040865822122 2304 36-37 4.627356551578643 2304 38-39 4.199774511383705 2304 40-41 3.628416499428994 2304 42-43 3.1758907097645412 2304 44-45 3.0512573388748407 2304 46-47 3.9514245758341815 2304 48-49 3.6351431347269383 2304 50-51 3.3139929911506485 2304 52-53 3.8945028523309126 2304 54-55 3.6700962295495394 2304 56-57 4.256227282830711 2304 58-59 3.500135933229597 2304 60-61 3.5641986057957205 2304 62-63 3.2676002199780108 2304 64-65 3.2076506375946607 2304 66-67 3.051022652893135 2304 68-69 3.212870875683805 2304 70-71 3.0851569430627457 2304 72-73 2.736546444748356 2304 74-75 2.1922858029112575 2304 76-77 2.4265648121560446 2304 78-79 2.825056186167213 2304 80-81 2.5891734035415688 2304 82-83 2.86487802877625 2304 84-85 2.3095414607425617 2304 86-87 2.172950290900502 2304 88-89 2.41067018265559 2304 90-91 2.3691172832347043 2304 92-93 2.025925293960835 2304 94-95 2.370688068433342 2304 96-97 2.320096824155222 2304 98-99 2.1961236428293915 2304 100-101 1.8302959412883837 1102 1 6.946045928898265 1102 2 7.449509934888038 1102 3 6.038589402362941 1102 4 6.082802433571054 1102 5 6.451147756647586 1102 6 5.727408666586474 1102 7 6.752245872672361 1102 8 6.292501212572098 1102 9 4.859219558466844 1102 10-11 5.897133594385338 1102 12-13 5.767894108404654 1102 14-15 6.132611738508185 1102 16-17 5.263305404570755 1102 18-19 5.1933389699996795 1102 20-21 5.425187164526893 1102 22-23 5.1238948150644745 1102 24-25 1.768456400127583 1102 26-27 5.337101828993703 1102 28-29 4.793780768160779 1102 30-31 4.831343864299765 1102 32-33 4.9052272876737035 1102 34-35 4.7914489959736635 1102 36-37 4.79693907223357 1102 38-39 3.908388126621862 1102 40-41 3.8475418119297657 1102 42-43 4.0918925978919205 1102 44-45 -3.6643142176418877 1102 46-47 1.304812801567543 1102 48-49 -0.7658237869312963 1102 50-51 2.5671192609382523 1102 52-53 -0.3270519333374047 1102 54-55 2.5270739725589557 1102 56-57 -0.8152148364819816 1102 58-59 2.7545546578449596 1102 60-61 3.732380885783673 1102 62-63 3.408046789094012 1102 64-65 2.9517486260876 1102 66-67 2.9443403152224406 1102 68-69 3.344830193775696 1102 70-71 3.218385783435405 1102 72-73 2.597762449434569 1102 74-75 2.4266559096664935 1102 76-77 2.6176936008809975 1102 78-79 -1.422579904229071 1102 80-81 1.5220781850823109 1102 82-83 2.521277432742284 1102 84-85 2.283887133076938 1102 86-87 2.2224657759486846 1102 88-89 2.2098895883001894 1102 90-91 2.0149076807878323 1102 92-93 1.9714696807875534 1102 94-95 2.3506585395163277 1102 96-97 2.281847128502676 1102 98-99 2.271043542411512 1102 100-101 1.959341061870557 2202 1 -20.422868563364243 2202 2 -7.557506233739602 2202 3 -0.7117805991572759 2202 4 1.454890251289381 2202 5 1.0980381512251753 2202 6 1.5709334906231263 2202 7 3.9238798354795748 2202 8 2.582752225454257 2202 9 3.142999216823206 2202 10-11 2.073947059973534 2202 12-13 1.4410278113262507 2202 14-15 2.6702085084044285 2202 16-17 1.6622810983396654 2202 18-19 1.6425573583299808 2202 20-21 1.0207765012395598 2202 22-23 1.3294157197208634 2202 24-25 2.3939328299991587 2202 26-27 3.5302750018396623 2202 28-29 -1.0013954802846001 2202 30-31 0.8927239366157913 2202 32-33 1.6535074008724528 2202 34-35 1.603326567144105 2202 36-37 2.8832558664037578 2202 38-39 2.3974466962118193 2202 40-41 1.7804727582591653 2202 42-43 0.6530567024928828 2202 44-45 0.6749330650281351 2202 46-47 -1.2716627306918404 2202 48-49 0.7553199685534118 2202 50-51 1.7174273347396394 2202 52-53 1.5937264546424892 2202 54-55 -0.8267362684417243 2202 56-57 1.0228048190487797 2202 58-59 1.860673324175945 2202 60-61 2.06417932920645 2202 62-63 -2.141493698905265 2202 64-65 -0.04725104414509218 2202 66-67 0.4570946532505502 2202 68-69 1.4165009532035278 2202 70-71 -2.6070896821771186 2202 72-73 -4.432875272187523 2202 74-75 -1.1971144007942698 2202 76-77 -0.27699076865315675 2202 78-79 1.3632370725312377 2202 80-81 1.4708599095532868 2202 82-83 -0.7712288009680428 2202 84-85 -0.2814413091877981 2202 86-87 0.46829916306693065 2202 88-89 1.0869513033867442 2202 90-91 1.2877305316656766 2202 92-93 1.248459338018069 2202 94-95 1.5394666292751964 2202 96-97 1.0838404407857567 2202 98-99 1.1717868096932555 2202 100-101 0.8632117091347808 2305 1 5.542370337820497 2305 2 6.972984773296975 2305 3 6.659205628920827 2305 4 7.698288372644516 2305 5 7.631079039774754 2305 6 6.077812512074878 2305 7 2.8202301561182175 2305 8 5.867007748927971 2305 9 6.291428576795738 2305 10-11 4.522217901503289 2305 12-13 5.019712570824879 2305 14-15 5.4764812354793015 2305 16-17 4.900765253220808 2305 18-19 4.283792707919524 2305 20-21 5.005045768127932 2305 22-23 4.972846055325988 2305 24-25 5.13647336156604 2305 26-27 5.951866377226153 2305 28-29 5.01448488015108 2305 30-31 5.293568393094244 2305 32-33 5.164101033254454 2305 34-35 5.12180750844956 2305 36-37 4.601876853532723 2305 38-39 4.56165964721087 2305 40-41 3.957013190068551 2305 42-43 3.893031395272086 2305 44-45 3.07098722614127 2305 46-47 3.768596171050202 2305 48-49 3.30823074136039 2305 50-51 2.2150603687279364 2305 52-53 3.8084902946481627 2305 54-55 4.0373072607201586 2305 56-57 4.536265150405647 2305 58-59 4.179942363969733 2305 60-61 4.338493729225942 2305 62-63 3.6815873812828848 2305 64-65 3.8987698662533745 2305 66-67 3.4482134977875916 2305 68-69 3.790954057529426 2305 70-71 3.5122788047082487 2305 72-73 -1.0353449070725915 2305 74-75 1.9391244219331263 2305 76-77 2.940985764002491 2305 78-79 3.48079697314337 2305 80-81 3.0364825827480537 2305 82-83 3.3078642292832754 2305 84-85 2.792722448450153 2305 86-87 2.6333558783786977 2305 88-89 2.7929250130765197 2305 90-91 2.734840122446233 2305 92-93 2.782030081228182 2305 94-95 3.0201501297457405 2305 96-97 2.878424067234036 2305 98-99 2.7617938219744502 2305 100-101 2.290909502720897 1103 1 -20.422868563364243 1103 2 -7.60042395368998 1103 3 -0.5723245555699137 1103 4 2.8332619170331412 1103 5 4.626637613929049 1103 6 4.446392147717177 1103 7 3.9928181157087295 1103 8 4.3530079001202715 1103 9 4.487754103046726 1103 10-11 4.942667318287761 1103 12-13 4.5590383998540105 1103 14-15 4.071049335499769 1103 16-17 2.8847047206325236 1103 18-19 3.161174545645828 1103 20-21 2.436850715822583 1103 22-23 2.8022797650336955 1103 24-25 2.9981997840732433 1103 26-27 4.098647884569015 1103 28-29 3.696253523447762 1103 30-31 3.544006881172681 1103 32-33 2.965308015404993 1103 34-35 2.520777082484038 1103 36-37 2.8050002284883035 1103 38-39 2.323924254914619 1103 40-41 2.5858888298944294 1103 42-43 2.351671142554121 1103 44-45 2.6965406781466044 1103 46-47 1.932284437071658 1103 48-49 -1.06445060255097 1103 50-51 -1.809245698508846 1103 52-53 1.7815325037386707 1103 54-55 2.3237071556225537 1103 56-57 3.236401869244389 1103 58-59 2.7677029108579667 1103 60-61 3.0906674047432965 1103 62-63 2.887609634242054 1103 64-65 2.8870121158860265 1103 66-67 2.455729554635184 1103 68-69 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-19.246322483946877 1209 30-31 -25.40913958763743 1209 32-33 -25.085736227518886 1209 34-35 -22.291385662026194 1209 36-37 -25.489517496161326 1209 38-39 -25.77273691573666 1209 40-41 -22.445947073450643 1209 42-43 -23.079650914579457 1209 44-45 -25.81307734878387 1209 46-47 -19.302715110131622 1209 48-49 -19.08272602474488 1209 50-51 -21.364513441894893 1209 52-53 -23.973894811457814 1209 54-55 -21.1006551036145 1209 56-57 -24.805161747133738 1209 58-59 -25.082393269411337 1209 60-61 -24.82483135865324 1209 62-63 -24.95719663121549 1209 64-65 -24.82561497946984 1209 66-67 -12.804384596979897 1209 68-69 -24.526908199989126 1209 70-71 -24.36983289949693 1209 72-73 -14.87663230504003 1209 74-75 -15.988680635213 1209 76-77 -13.100323307871044 1209 78-79 -13.645511043390936 1209 80-81 -23.453297393817497 1209 82-83 -15.226345836976247 1209 84-85 -12.627408344573166 1209 86-87 -12.94615098724407 1209 88-89 -10.590567755384027 1209 90-91 -13.109083897567736 1209 92-93 -16.970805279934545 1209 94-95 -22.244789331261938 1209 96-97 -18.055151821519402 1209 98-99 -9.661106126935294 1209 100-101 -5.263882575725326 1212 1 -20.422868563364243 1212 2 -20.239747943797152 1212 3 -21.109834860598767 1212 4 -23.034558912865183 1212 5 -23.209306326713985 1212 6 -24.25847182590195 1212 7 -23.812760339910945 1212 8 -23.46328778550765 1212 9 -24.363303879915627 1212 10-11 -24.078354940574563 1212 12-13 -24.3530253539245 1212 14-15 -24.64576847159009 1212 16-17 -25.677675054030846 1212 18-19 -20.323146196815888 1212 20-21 -26.063630185523937 1212 22-23 -26.24581036961868 1212 24-25 -22.4238731826205 1212 26-27 -24.975617023372727 1212 28-29 -19.847059584683976 1212 30-31 -14.6539184574163 1212 32-33 -25.085736227518886 1212 34-35 -21.214041272181802 1212 36-37 -25.489517496161326 1212 38-39 -25.77273691573666 1212 40-41 -20.249809174187746 1212 42-43 -16.59233263038617 1212 44-45 -13.88371616942269 1212 46-47 -17.006710298501808 1212 48-49 -19.571938248332103 1212 50-51 -11.792287807169256 1212 52-53 -4.907555745118749 1212 54-55 -3.486980349314745 1212 56-57 -3.5121642041361945 1212 58-59 -2.4193097313277967 1212 60-61 -13.828209737031617 1212 62-63 -11.528756827775686 1212 64-65 -15.771868050722912 1212 66-67 -11.9614408009111 1212 68-69 -7.473468396549325 1212 70-71 -14.775852555516586 1212 72-73 -11.438300507333231 1212 74-75 -8.031857334639703 1212 76-77 -18.52616534308808 1212 78-79 -9.588500908255801 1212 80-81 -7.407535723055826 1212 82-83 -5.206369895125306 1212 84-85 -4.346951751616572 1212 86-87 -3.1725279323710183 1212 88-89 -3.047109524425796 1212 90-91 -3.315049806658646 1212 92-93 -3.35701535364462 1212 94-95 -3.1286959651685713 1212 96-97 -12.722816645434225 1212 98-99 -9.95412926370843 1212 100-101 -6.2750926494354005 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 833.0 3 1682.0 4 856.0 5 4925.0 6 6398.0 7 7040.0 8 15629.0 9 29331.0 10 38159.0 11 43676.0 12 54910.0 13 66483.0 14 75363.0 15 80414.0 16 84744.0 17 92731.0 18 103944.0 19 113416.0 20 122623.0 21 134390.0 22 148022.0 23 158757.0 24 166709.0 25 170973.0 26 178213.0 27 185036.0 28 188342.0 29 190065.0 30 196011.0 31 206357.0 32 222873.0 33 253530.0 34 285854.0 35 311149.0 36 322098.0 37 295815.0 38 106422.0 39 318.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.11104701449524 32.20029665447948 14.983882170608856 20.70477416041642 2 24.27549842879177 29.065050984447915 24.70574032570191 21.953710261058397 3 21.56101329791465 27.375963176978107 28.51146315050432 22.55156037460292 4 24.298694451595185 25.553897878062276 26.928133817195075 23.21927385314746 5 22.952587351042833 28.165866998252802 26.083646063260126 22.797899587444242 6 22.8160445971161 27.411362280447037 26.38329746314491 23.38929565929195 7 23.835397703074786 27.918709584045608 27.051401442310546 21.19449127056906 8 21.913656272646147 26.782497414081025 28.221249845750112 23.082596467522716 9 23.0349490229002 26.96639960760519 27.060577082393166 22.938074287101447 10-11 23.465662636531718 27.182332043488632 26.468593296807324 22.883412023172323 12-13 23.067400440227896 27.1810714688563 26.73137581863227 23.020152272283536 14-15 22.96365924570372 27.458245300409565 26.793676966215134 22.784418487671573 16-17 23.12422498363648 27.521686691123566 26.7114239132865 22.64266441195346 18-19 23.40330803162249 27.42283686304813 26.368569406660054 22.805285698669326 20-21 23.20141493919022 27.42064026617703 26.510744207847065 22.86720058678569 22-23 23.1255704662861 27.187417670006838 26.764803741893694 22.92220812181337 24-25 23.12290573412579 27.145440218213533 26.7842317705701 22.947422277090578 26-27 22.912053280645413 27.3712421373114 26.7909385797788 22.925766002264393 28-29 23.304630840922655 27.293178939237055 26.641699280655608 22.760490939184685 30-31 23.355542376011513 27.172405836568874 26.468025251499476 23.00402653592013 32-33 22.96858919701385 27.312834515824164 26.743142380682837 22.975433906479154 34-35 23.207576950325404 27.205461960091483 26.734183157760487 22.85277793182263 36-37 23.205573710702854 27.305283787419672 26.58326787564429 22.905874626233185 38-39 23.07760712743166 27.310533416983734 26.807630856039953 22.804228599544647 40-41 23.360921045596456 27.260493863177338 26.54486109494872 22.833723996277485 42-43 23.268833104188207 27.179156127122884 26.632000043985514 22.920010724703396 44-45 23.029668561985257 27.175453978816872 26.842440061676324 22.952437397521543 46-47 23.22502850174814 27.392315559183107 26.574236593310214 22.808419345758537 48-49 23.22998884048406 27.169616065534008 26.680905480886764 22.919489613095166 50-51 23.20540648465494 27.282152142677823 26.565015836809984 22.947425535857253 52-53 23.12410040032529 27.267046142826583 26.798995987953088 22.80985746889504 54-55 23.158224402625404 27.11314402585017 26.70173859402783 23.026892977496598 56-57 22.9878259908669 27.30103797538293 26.82359266648373 22.887543367266435 58-59 23.170940541501515 27.28893465079399 26.66890489442021 22.87121991328429 60-61 23.259243716208207 27.04505499642852 26.600267235783935 23.095434051579332 62-63 23.04469139661734 27.179355083116867 26.7604403200174 23.015513200248396 64-65 23.190253389383972 27.16943478750771 26.68154629768134 22.958765525426976 66-67 23.229708224642106 27.122213595092965 26.614070769943073 23.034007410321856 68-69 23.121916506949468 27.174037219538384 26.773671515519776 22.93037475799237 70-71 23.34468160037531 27.241071564907504 26.525759288300456 22.88848754641673 72-73 23.218355656127045 27.11630921226986 26.592847478613358 23.072487652989732 74-75 23.115617257624404 27.117597073547028 26.682408893374337 23.08437677545423 76-77 23.32313766627643 27.29928626740578 26.51234950172267 22.865226564595115 78-79 23.420778612890302 27.063366986820377 26.50127747125581 23.01457692903351 80-81 23.32811047332702 27.12625469328967 26.502640262713477 23.042994570669833 82-83 23.33546239681692 27.116500285096258 26.602669776650895 22.945367541435928 84-85 23.41754899605989 26.94277223302185 26.42955705757491 23.210121713343348 86-87 23.232711181393302 27.169258036379617 26.59443067989437 23.003600102332705 88-89 23.42153796369822 27.104289698387134 26.529187654549087 22.94498468336556 90-91 23.484500978738158 26.99336552551903 26.277137142337807 23.244996353405003 92-93 23.345491291211637 27.034598365897438 26.471419850098982 23.14849049279194 94-95 23.49076446394474 27.001011272620357 26.376359267791237 23.131864995643667 96-97 23.497261468063158 26.987416881270455 26.377580934941186 23.1377407157252 98-99 23.459164917515817 27.05863951787159 26.387568488293834 23.094627076318762 100-101 23.828657447817445 26.9950977963885 26.01548447742978 23.160760278364272 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 333.0 1 343.5 2 386.5 3 438.5 4 449.0 5 488.0 6 610.0 7 750.5 8 950.0 9 1223.5 10 1559.0 11 2084.0 12 2767.0 13 3609.0 14 4723.5 15 6047.5 16 7586.0 17 9508.5 18 12069.5 19 15305.0 20 18837.5 21 23296.0 22 28932.0 23 35084.0 24 42075.0 25 49921.5 26 58349.5 27 67274.0 28 76085.5 29 85287.5 30 94357.5 31 102801.5 32 111008.5 33 118287.5 34 125621.0 35 132467.5 36 137795.0 37 141727.5 38 143774.0 39 143852.5 40 141263.0 41 137205.5 42 132443.5 43 126582.5 44 120479.5 45 114520.5 46 109137.5 47 103947.5 48 98226.5 49 91894.5 50 86098.5 51 81521.5 52 77436.0 53 73983.5 54 71884.5 55 70816.0 56 70145.0 57 69672.0 58 69558.0 59 69637.0 60 69365.0 61 68577.0 62 67646.5 63 66704.0 64 65677.0 65 64639.0 66 62917.0 67 60553.0 68 57558.5 69 53912.0 70 49597.5 71 44445.5 72 38948.5 73 33342.0 74 27954.0 75 22779.0 76 18098.5 77 14305.0 78 11218.0 79 8738.5 80 6821.5 81 5389.0 82 4293.5 83 3423.5 84 2739.5 85 2206.0 86 1763.0 87 1394.0 88 1090.5 89 882.0 90 720.5 91 564.0 92 441.5 93 334.5 94 252.0 95 173.0 96 111.5 97 75.5 98 44.0 99 27.5 100 13.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 13.661483019949653 2 11.022512210846658 3 9.69916753339504 4 11.632020044205827 5 9.130953920067169 6 5.340740564452966 7 9.63373141733298 8 9.998882954899464 9 5.407441664410064 10-11 6.371166428785373 12-13 5.340912087692971 14-15 7.314061839702528 16-17 2.664345528421294 18-19 3.109480496842793 20-21 3.070051592046553 22-23 3.115494530445482 24-25 3.490755218969784 26-27 4.311279518345589 28-29 1.7462352256849192 30-31 2.2477477390556917 32-33 3.052052372048487 34-35 1.9920601892201504 36-37 3.060028202708738 38-39 1.3891345601961882 40-41 1.1527540950637543 42-43 0.561749331220167 44-45 1.294560933738214 46-47 1.3613799559228155 48-49 1.2565042148620171 50-51 1.5289152805980843 52-53 2.4772243937779086 54-55 1.794443976328935 56-57 1.8231312382198372 58-59 1.5898060307999995 60-61 1.3969710282239347 62-63 0.6170655761219067 64-65 1.2439294173291215 66-67 0.5769720187706457 68-69 0.8135668879530867 70-71 1.0095536300642505 72-73 0.08696228268273497 74-75 0.10874573416342005 76-77 0.4630269863945622 78-79 0.8768053625025755 80-81 0.29376570911673894 82-83 0.5049858589808818 84-85 0.06819120810464462 86-87 0.10246369549822248 88-89 0.08493616441017124 90-91 0.09143260712537556 92-93 0.17191988749790688 94-95 0.4593821175444475 96-97 0.3719803065591988 98-99 0.06985283949219687 100-101 0.23540492670490348 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4664091.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.10272532835967 #Duplication Level Percentage of deduplicated Percentage of total 1 98.54331965765417 95.68824901661938 2 0.9533000179955552 1.8513605960588548 3 0.18910447862935545 0.550876807401269 4 0.07097501400673846 0.2756746916109122 5 0.0671611987355636 0.3260767716771405 6 0.06247899489980188 0.36401284083284685 7 0.040661557755452486 0.27638436519056553 8 0.025897708943474766 0.20117904945376272 9 0.018719923278285172 0.16359800114333625 >10 0.028381448101599382 0.3025878600119451 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 4.2880810001348604E-5 0.0 0.0 2 1.072020250033715E-4 0.0 0.0 3 1.072020250033715E-4 2.1440405000674302E-5 0.0 4 1.072020250033715E-4 2.1440405000674302E-5 2.1440405000674302E-5 5 1.7152324000539441E-4 4.2880810001348604E-5 2.1440405000674302E-5 6 2.14404050006743E-4 4.2880810001348604E-5 2.1440405000674302E-5 7 2.358444550074173E-4 4.2880810001348604E-5 2.1440405000674302E-5 8 2.358444550074173E-4 4.2880810001348604E-5 4.2880810001348604E-5 9 2.5728486000809164E-4 4.2880810001348604E-5 4.2880810001348604E-5 10-11 3.001656700094402E-4 6.432121500202291E-5 6.432121500202291E-5 12-13 3.001656700094402E-4 6.432121500202291E-5 6.432121500202291E-5 14-15 3.216060750101145E-4 6.432121500202291E-5 9.648182250303436E-5 16-17 3.3232627751045166E-4 6.432121500202291E-5 1.2864243000404582E-4 18-19 3.4304648001078883E-4 6.432121500202291E-5 1.7152324000539441E-4 20-21 3.644868850114631E-4 6.432121500202291E-5 1.7152324000539441E-4 22-23 3.644868850114631E-4 7.504141750236005E-5 1.7152324000539441E-4 24-25 3.859272900121374E-4 1.072020250033715E-4 1.7152324000539441E-4 26-27 4.0736769501281174E-4 1.2864243000404582E-4 1.929636450060687E-4 28-29 4.716889100148346E-4 1.2864243000404582E-4 1.929636450060687E-4 30-31 5.252899225165204E-4 1.2864243000404582E-4 2.14404050006743E-4 32-33 6.324919475198919E-4 1.500828350047201E-4 2.358444550074173E-4 34-35 7.075333650222519E-4 1.8224344250573156E-4 2.4656465750775447E-4 36-37 8.254555925259605E-4 2.14404050006743E-4 2.787252650087659E-4 38-39 9.96978832531355E-4 2.2512425250708015E-4 3.001656700094402E-4 40-41 0.0012006626800377608 2.6800506250842875E-4 3.001656700094402E-4 42-43 0.0016723515900525954 2.787252650087659E-4 3.216060750101145E-4 44-45 0.002261962727571139 2.787252650087659E-4 3.4304648001078883E-4 46-47 0.002990936497594065 2.787252650087659E-4 3.4304648001078883E-4 48-49 0.0038056718876196887 2.787252650087659E-4 3.4304648001078883E-4 50-51 0.004824091125151718 2.787252650087659E-4 3.9664749251247457E-4 52-53 0.006249878057696558 2.787252650087659E-4 4.180878975131489E-4 54-55 0.00835103774776264 2.787252650087659E-4 4.502485050141603E-4 56-57 0.010505798450330407 2.787252650087659E-4 4.609687075144975E-4 58-59 0.012681999557898848 2.787252650087659E-4 4.931293150155089E-4 60-61 0.015705096662993925 2.787252650087659E-4 4.931293150155089E-4 62-63 0.019703732195619682 2.787252650087659E-4 5.145697200161833E-4 64-65 0.024270538460763307 2.787252650087659E-4 5.360101250168575E-4 66-67 0.02979144274843694 2.787252650087659E-4 5.360101250168575E-4 68-69 0.03653445012114901 2.787252650087659E-4 5.360101250168575E-4 70-71 0.04499268989391502 2.787252650087659E-4 5.574505300175317E-4 72-73 0.05394405898169654 2.787252650087659E-4 5.788909350182061E-4 74-75 0.06442841702702627 2.787252650087659E-4 5.788909350182061E-4 76-77 0.07616703876489546 2.787252650087659E-4 5.896111375185433E-4 78-79 0.09174349299788534 2.787252650087659E-4 6.217717450195547E-4 80-81 0.11114705952349557 2.787252650087659E-4 6.217717450195547E-4 82-83 0.1331663554591881 3.001656700094402E-4 6.43212150020229E-4 84-85 0.15643991508742003 3.53766682511126E-4 6.646525550209033E-4 86-87 0.18294025566825348 3.859272900121374E-4 6.753727575212404E-4 88-89 0.21361075502171806 3.859272900121374E-4 6.860929600215777E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 2780 0.0 5.141317 94-95 >>END_MODULE