##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	NYCLib28_CAAAAG_L007_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4664091
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.238833676272613	33.0	33.0	33.0	27.0	33.0
2	31.673307188903475	33.0	33.0	33.0	27.0	33.0
3	31.82857752989811	33.0	33.0	33.0	27.0	33.0
4	35.64170231670008	37.0	37.0	37.0	33.0	37.0
5	35.15643970068337	37.0	37.0	37.0	33.0	37.0
6	34.96169585885009	37.0	37.0	37.0	33.0	37.0
7	34.760052494687606	37.0	33.0	37.0	27.0	37.0
8	34.8076077417872	37.0	33.0	37.0	27.0	37.0
9	34.71522339508385	37.0	33.0	37.0	27.0	37.0
10-11	34.56495456456574	37.0	33.0	37.0	27.0	37.0
12-13	34.5754844834717	37.0	33.0	37.0	27.0	37.0
14-15	35.5222684334418	37.0	33.0	40.0	27.0	40.0
16-17	35.43020558561143	37.0	33.0	40.0	27.0	40.0
18-19	35.646569288635234	37.0	33.0	40.0	27.0	40.0
20-21	35.31673224214536	37.0	33.0	40.0	27.0	40.0
22-23	35.344179819819125	37.0	33.0	40.0	27.0	40.0
24-25	35.18237937467343	37.0	33.0	40.0	27.0	40.0
26-27	34.79873848945057	37.0	33.0	40.0	27.0	40.0
28-29	34.42888850153224	37.0	33.0	40.0	27.0	40.0
30-31	34.32248105793819	37.0	33.0	40.0	24.5	40.0
32-33	34.323236725012435	37.0	33.0	40.0	24.5	40.0
34-35	34.16994351096494	37.0	33.0	38.5	24.5	40.0
36-37	33.86757687618016	37.0	33.0	37.0	22.0	40.0
38-39	33.764478115885815	37.0	33.0	37.0	22.0	40.0
40-41	33.45812185053851	37.0	33.0	37.0	22.0	40.0
42-43	33.078622715551646	37.0	33.0	37.0	22.0	40.0
44-45	32.94521322589975	37.0	33.0	37.0	22.0	40.0
46-47	32.80023867458847	37.0	33.0	37.0	22.0	40.0
48-49	32.81970441828858	37.0	33.0	37.0	22.0	40.0
50-51	33.10559570986072	37.0	33.0	37.0	22.0	40.0
52-53	32.96844422632405	37.0	33.0	37.0	22.0	40.0
54-55	32.60454116354076	37.0	33.0	37.0	22.0	40.0
56-57	32.650636640665894	37.0	33.0	37.0	22.0	40.0
58-59	32.366620098107006	37.0	33.0	37.0	22.0	40.0
60-61	32.27557781355466	37.0	27.0	37.0	22.0	40.0
62-63	32.252727701925195	33.0	27.0	37.0	22.0	40.0
64-65	31.915133731310128	33.0	27.0	37.0	22.0	40.0
66-67	31.57076930960395	33.0	27.0	37.0	18.5	40.0
68-69	31.40096387484721	33.0	27.0	37.0	15.0	40.0
70-71	31.24187971461106	33.0	27.0	37.0	15.0	38.5
72-73	31.025062439819465	33.0	27.0	37.0	15.0	37.0
74-75	30.68070884551781	33.0	27.0	37.0	15.0	37.0
76-77	29.61202247125967	33.0	27.0	37.0	15.0	37.0
78-79	30.541636301693085	33.0	27.0	37.0	15.0	37.0
80-81	30.25607058267088	33.0	27.0	37.0	15.0	37.0
82-83	30.23194240849932	33.0	27.0	37.0	15.0	37.0
84-85	30.11186445547482	33.0	27.0	37.0	15.0	37.0
86-87	29.916959381795937	33.0	27.0	37.0	15.0	37.0
88-89	29.78104876169869	33.0	27.0	37.0	15.0	37.0
90-91	29.688111895758468	33.0	27.0	37.0	15.0	37.0
92-93	29.47989962031187	33.0	27.0	35.0	15.0	37.0
94-95	29.381064820562035	33.0	27.0	33.0	15.0	37.0
96-97	29.24648532800925	33.0	27.0	33.0	15.0	37.0
98-99	28.988171650167203	33.0	27.0	33.0	15.0	37.0
100-101	28.184404742531825	33.0	24.5	33.0	15.0	37.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
2306	1	1.0953705734377053
2306	2	0.5580194880519684
2306	3	0.5352373634481467
2306	4	0.41764871758171296
2306	5	0.6440949403359681
2306	6	0.7628894133918465
2306	7	0.8607508339078578
2306	8	0.8096696858398431
2306	9	1.0230031675897209
2306	10-11	1.1596361846495284
2306	12-13	1.3248080022224258
2306	14-15	1.5048622331933714
2306	16-17	1.4556285186973028
2306	18-19	1.3650503517138048
2306	20-21	1.6449554459348477
2306	22-23	1.2712060378176133
2306	24-25	1.2503612238665127
2306	26-27	1.4048767094777332
2306	28-29	1.648235425963719
2306	30-31	1.5607348855811338
2306	32-33	1.223242851167825
2306	34-35	1.1447696370759317
2306	36-37	1.4007992540446565
2306	38-39	1.0566632432076517
2306	40-41	1.2903980688236913
2306	42-43	1.2142353789401312
2306	44-45	1.0562423179297014
2306	46-47	1.0103755290868648
2306	48-49	1.044070341811981
2306	50-51	1.0848851218774698
2306	52-53	1.2064561317404525
2306	54-55	1.6262797125785724
2306	56-57	1.2427607329223846
2306	58-59	1.0882998670405115
2306	60-61	0.9054620345471633
2306	62-63	0.640814013988674
2306	64-65	0.8086018123315704
2306	66-67	0.970929209397724
2306	68-69	0.831767122799139
2306	70-71	0.7362556142132064
2306	72-73	0.4364961733290684
2306	74-75	0.7376063226132139
2306	76-77	0.8063353143770691
2306	78-79	0.815796985859329
2306	80-81	1.2657356970624143
2306	82-83	1.0929390399892398
2306	84-85	0.8434783652911619
2306	86-87	0.6487359333262148
2306	88-89	0.566993856109697
2306	90-91	0.2481754261329172
2306	92-93	0.0580533331474129
2306	94-95	0.08011261691340721
2306	96-97	0.1253309937683582
2306	98-99	0.2906166116519273
2306	100-101	0.26912291034166813
2307	1	0.6030618407552417
2307	2	0.48190563981077617
2307	3	0.3305444862031166
2307	4	0.2910413381854724
2307	5	0.5443954497403283
2307	6	0.6463632865156796
2307	7	0.7321193814087081
2307	8	0.7741288751231536
2307	9	0.9718501917378504
2307	10-11	1.0891991675814836
2307	12-13	1.2969585172881466
2307	14-15	1.2753173061604386
2307	16-17	1.2906239009474092
2307	18-19	1.212616147585294
2307	20-21	1.3654141339793853
2307	22-23	1.179355026560593
2307	24-25	1.1966297720884285
2307	26-27	1.2667174231232607
2307	28-29	1.536569415077949
2307	30-31	1.5852404530927515
2307	32-33	1.2262097676548578
2307	34-35	1.193811602571138
2307	36-37	1.285679937974436
2307	38-39	0.9898431744802565
2307	40-41	1.1627309455464285
2307	42-43	1.228645730573163
2307	44-45	1.048076579002995
2307	46-47	1.0150670129192818
2307	48-49	1.072964701063377
2307	50-51	1.0062409142936772
2307	52-53	1.2478210271360908
2307	54-55	1.5417841854330518
2307	56-57	1.2117481623918351
2307	58-59	1.1515034843786154
2307	60-61	0.9687885937515119
2307	62-63	0.9375251412360868
2307	64-65	0.9154177047188021
2307	66-67	1.038765261054941
2307	68-69	0.8968514476478511
2307	70-71	0.8121722507217441
2307	72-73	0.782658081637841
2307	74-75	0.8408836875316048
2307	76-77	1.0119300024153723
2307	78-79	1.0373863356657225
2307	80-81	1.5590940104835909
2307	82-83	1.2470153467715406
2307	84-85	1.0695631564946169
2307	86-87	1.0347994686537056
2307	88-89	0.9364342518622628
2307	90-91	0.7392023556342302
2307	92-93	0.7404366806389895
2307	94-95	0.633114992340861
2307	96-97	0.5368284796817377
2307	98-99	0.5916665139473949
2307	100-101	0.7309046524270322
1101	1	1.0127573742863625
1101	2	0.5482272497882725
1101	3	0.38405618472854286
1101	4	0.3417968009661223
1101	5	0.5483069299021892
1101	6	0.538395913636883
1101	7	0.7453270396439606
1101	8	0.7029070703959945
1101	9	0.9200962302667293
1101	10-11	0.973440437630849
1101	12-13	1.1938050429732954
1101	14-15	1.2640403775187181
1101	16-17	1.2737228039794246
1101	18-19	0.9536405039183933
1101	20-21	1.249918944536553
1101	22-23	0.9699302805761079
1101	24-25	1.0497694706268206
1101	26-27	1.0581605410514712
1101	28-29	1.2446702059015564
1101	30-31	1.3237635405938022
1101	32-33	0.8658228802252168
1101	34-35	0.9730442203924454
1101	36-37	1.0045157301988326
1101	38-39	0.7643924584860216
1101	40-41	1.0549420375015046
1101	42-43	1.0134405407329652
1101	44-45	0.7819523377861657
1101	46-47	0.7735833539914196
1101	48-49	0.7713765191069157
1101	50-51	0.7432729965864198
1101	52-53	1.0944495854231562
1101	54-55	1.5006700954728487
1101	56-57	1.0391827922808403
1101	58-59	1.1218310921511048
1101	60-61	0.9184089185461062
1101	62-63	0.7090845715364011
1101	64-65	0.6486393192061861
1101	66-67	0.6865075823651132
1101	68-69	0.694943923784944
1101	70-71	0.782638526640568
1101	72-73	0.7068853786280158
1101	74-75	0.7716554887633009
1101	76-77	0.7604038424659834
1101	78-79	0.7215315814501828
1101	80-81	1.3454793575873367
1101	82-83	1.1485087509857905
1101	84-85	1.0396113671102896
1101	86-87	0.7911778132211502
1101	88-89	0.7268096520750831
1101	90-91	0.5574972830631957
1101	92-93	0.68647490338655
1101	94-95	0.7127244541803996
1101	96-97	0.602351194666582
1101	98-99	0.7082301614160542
1101	100-101	0.7011119897677283
2201	1	1.4305048579180308
2201	2	0.8107176084087122
2201	3	0.3264238927924694
2201	4	0.2104421985705116
2201	5	0.42965217447341786
2201	6	0.4578439023251377
2201	7	0.6038262388196856
2201	8	0.5842164147668711
2201	9	0.587560773178005
2201	10-11	0.7401662644162883
2201	12-13	1.0104272527551217
2201	14-15	1.0722667553178482
2201	16-17	0.9572357941103391
2201	18-19	0.8335735530666426
2201	20-21	1.1724352923233425
2201	22-23	0.9507821874952285
2201	24-25	0.9164406594025252
2201	26-27	1.1025968097949743
2201	28-29	1.3996254428644335
2201	30-31	1.3859194189413628
2201	32-33	1.0570982007956715
2201	34-35	0.8892145731689283
2201	36-37	0.9900974180022288
2201	38-39	0.6333868153049167
2201	40-41	0.9938538174128979
2201	42-43	1.0983652548700533
2201	44-45	0.9534231375664746
2201	46-47	0.8271963848051982
2201	48-49	0.8552340484164489
2201	50-51	0.8910081935659093
2201	52-53	1.0433314472921538
2201	54-55	1.3543439612108514
2201	56-57	1.0430882787783204
2201	58-59	1.1275025683298736
2201	60-61	0.8786290286779277
2201	62-63	0.8143757997233507
2201	64-65	0.8675115992271039
2201	66-67	0.8024063023799286
2201	68-69	0.738907436046194
2201	70-71	0.8379286931324792
2201	72-73	0.7163474183565235
2201	74-75	0.7932438788238514
2201	76-77	0.8774907442799034
2201	78-79	0.7992365577941278
2201	80-81	1.2522827799764045
2201	82-83	1.1121412134377486
2201	84-85	1.0265348601570743
2201	86-87	1.0736773459343105
2201	88-89	1.0509803221916059
2201	90-91	0.8866726063050159
2201	92-93	0.9828710269224032
2201	94-95	0.9752680385748604
2201	96-97	0.7572658071883893
2201	98-99	0.9589234865534522
2201	100-101	0.9659731819049959
2304	1	-1.8951545100705403
2304	2	-0.28661342294136816
2304	3	-0.16531373433864616
2304	4	-0.0347040943593413
2304	5	0.3218812291708133
2304	6	0.46820837524430203
2304	7	0.5614111167891238
2304	8	0.6527807295193
2304	9	0.902419178809204
2304	10-11	0.8875802783344255
2304	12-13	1.1680081082302394
2304	14-15	1.221833163308503
2304	16-17	1.2168855807537469
2304	18-19	0.8675881477857033
2304	20-21	1.1429863827789148
2304	22-23	0.9238160123057924
2304	24-25	1.0451546982775923
2304	26-27	1.1076106717316634
2304	28-29	1.4380687370478853
2304	30-31	1.4469750390954275
2304	32-33	0.8153148261397334
2304	34-35	0.8614828012072664
2304	36-37	1.0698835672031706
2304	38-39	0.8982535279263146
2304	40-41	0.8220121535777309
2304	42-43	0.8577275975625298
2304	44-45	0.9650640203699012
2304	46-47	0.8037145733121278
2304	48-49	0.8247304670608031
2304	50-51	0.7665211643377319
2304	52-53	0.9453561233169694
2304	54-55	1.330838479676153
2304	56-57	0.9621380207478936
2304	58-59	0.8117270031694162
2304	60-61	0.4109627293614011
2304	62-63	0.17704799767075485
2304	64-65	0.31111666549448813
2304	66-67	0.42811149752125033
2304	68-69	0.5039230271930926
2304	70-71	0.4236882078727362
2304	72-73	0.2919078550750811
2304	74-75	0.45322285756648384
2304	76-77	0.6385615425431332
2304	78-79	0.5237305403378407
2304	80-81	1.158752235168329
2304	82-83	0.9045903186099977
2304	84-85	0.5663013135202348
2304	86-87	0.4529488573143752
2304	88-89	0.36419000603588003
2304	90-91	0.2423677544576428
2304	92-93	0.3291386916060439
2304	94-95	0.12349339679005311
2304	96-97	0.07449097296087359
2304	98-99	0.4971867317333647
2304	100-101	0.41314516804213497
1102	1	1.411793902512457
1102	2	0.1247157687080076
1102	3	0.26451744769518015
1102	4	0.21177740181452265
1102	5	0.28996927775992987
1102	6	0.10186425004653188
1102	7	-4.965560226046172
1102	8	-1.2515063631368548
1102	9	0.09049426011146977
1102	10-11	0.6819630893478035
1102	12-13	0.9781615701384396
1102	14-15	0.8690585804069215
1102	16-17	0.6151596491573912
1102	18-19	0.8394669435238029
1102	20-21	1.1420676108642809
1102	22-23	0.6802588963235507
1102	24-25	1.0087415281861283
1102	26-27	1.0776339318454902
1102	28-29	1.0215973412339565
1102	30-31	1.2950056610874867
1102	32-33	1.0218108235999708
1102	34-35	1.2258386286872778
1102	36-37	1.3271451101622986
1102	38-39	0.9642723293449151
1102	40-41	1.187479557034571
1102	42-43	1.1873386730390365
1102	44-45	1.159807467555943
1102	46-47	0.9487307069828006
1102	48-49	0.9837755253995368
1102	50-51	0.7851428227472539
1102	52-53	0.9766177312157893
1102	54-55	1.4043947388789348
1102	56-57	1.174860297937478
1102	58-59	1.1419659485487657
1102	60-61	0.9586897840932309
1102	62-63	0.696239728506967
1102	64-65	0.5534992879991769
1102	66-67	0.703539454128915
1102	68-69	0.8740926569054572
1102	70-71	0.790930094240089
1102	72-73	0.7033845450230913
1102	74-75	0.8174879444431191
1102	76-77	0.7183900541327723
1102	78-79	0.7816680627863484
1102	80-81	1.3198804120718641
1102	82-83	1.1481046816700484
1102	84-85	0.8443040401269286
1102	86-87	0.7256091824935851
1102	88-89	0.635695550549201
1102	90-91	0.26181828317404054
1102	92-93	0.4553650665649762
1102	94-95	0.4832024362636922
1102	96-97	0.3100550780491389
1102	98-99	0.31542811143341254
1102	100-101	0.4314766056756163
2202	1	1.5095995491824965
2202	2	0.8983269052282985
2202	3	0.3667685297934966
2202	4	-0.27773056202109103
2202	5	0.05391666580504051
2202	6	0.6318907660869613
2202	7	0.6846270058205519
2202	8	-3.939185998948439
2202	9	-0.4077829665012018
2202	10-11	0.613721741220175
2202	12-13	0.046633481427100776
2202	14-15	0.21255600896175508
2202	16-17	0.2514078122284218
2202	18-19	0.8027752615319201
2202	20-21	1.2677783417549975
2202	22-23	-2.593915666200786
2202	24-25	-2.8507926737108917
2202	26-27	-1.4653453382565331
2202	28-29	0.6293235574130378
2202	30-31	1.2842848184779925
2202	32-33	1.198041415487559
2202	34-35	-2.06060941924844
2202	36-37	-0.20674359614164217
2202	38-39	0.38145808622032007
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2202	56-57	1.3398876313538253
2202	58-59	1.4121364601945245
2202	60-61	1.314618283990626
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2202	64-65	1.2119958171464127
2202	66-67	1.307984403558244
2202	68-69	1.203079526792738
2202	70-71	1.2450839101815134
2202	72-73	1.07416943672132
2202	74-75	0.8303756767989832
2202	76-77	1.2971156299385918
2202	78-79	1.2547380719865906
2202	80-81	1.8335190127948522
2202	82-83	1.708573606572596
2202	84-85	1.5414183161539583
2202	86-87	1.4577977546029999
2202	88-89	1.488972639874909
2202	90-91	1.2368048926649422
2202	92-93	1.2950225385340595
2202	94-95	1.3401830851787686
2202	96-97	1.1898771046170609
2202	98-99	1.4000863413441564
2202	100-101	1.229996170863199
2305	1	1.261338356942698
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2305	3	0.10690756733845319
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2305	5	0.2668845943766769
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2305	9	0.45381171415510835
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2305	98-99	0.8966027318111252
2305	100-101	1.029535436444661
1103	1	1.2515432890530285
1103	2	0.5784164485582188
1103	3	0.1988784763086322
1103	4	-0.28752153495513255
1103	5	0.12567585237066936
1103	6	0.2919102137577454
1103	7	0.43012368844283344
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1103	9	0.05006339373662172
1103	10-11	0.6007345054862228
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1103	14-15	0.1999626367586913
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1103	28-29	0.24850946709960198
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1103	54-55	-4.346584337803517
1103	56-57	-0.4751940057116997
1103	58-59	-2.707476637040589
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1103	64-65	0.09740662622268204
1103	66-67	0.1557998278113999
1103	68-69	-0.23589898173870338
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1103	80-81	1.3027746694621314
1103	82-83	0.7553309687894583
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1103	86-87	-2.6342519932979833
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1103	98-99	0.5366532302537834
1103	100-101	0.7828640282930337
2203	1	1.3040816916678217
2203	2	0.7317774538719242
2203	3	-0.16893415857198946
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2310	1	1.2083778734530206
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2204	98-99	0.6377163725914343
2204	100-101	-5.132651768360251
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2206	1	1.2695492018527368
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1209	92-93	-3.9026599159781803
1209	94-95	-0.8244999797124031
1209	96-97	-0.15895821867616533
1209	98-99	1.2108765277381082
1209	100-101	0.36998133699422553
1212	1	-28.633727389704873
1212	2	-16.292031868303948
1212	3	-5.6468862159111275
1212	4	-1.3178667643935214
1212	5	-0.4963520625261708
1212	6	-2.4994319415580293
1212	7	-0.3774143262262655
1212	8	-1.2214934939169382
1212	9	0.12652113754803906
1212	10-11	0.8846171196450143
1212	12-13	0.12924168817890802
1212	14-15	0.06831855169333068
1212	16-17	0.09616131407975814
1212	18-19	-1.036750040072036
1212	20-21	-0.6394076160955322
1212	22-23	-0.503108754004451
1212	24-25	0.3340583810360229
1212	26-27	-0.3222395060235428
1212	28-29	0.11033515023608231
1212	30-31	-0.6983261152839404
1212	32-33	0.17870068533564876
1212	34-35	-1.1650655221287707
1212	36-37	-1.2177555828661468
1212	38-39	-0.3707573570063545
1212	40-41	-0.45118181235366706
1212	42-43	0.2953382656201242
1212	44-45	1.3954272174928732
1212	46-47	-1.9592018474255255
1212	48-49	-1.1500989840256253
1212	50-51	0.156645798875779
1212	52-53	-0.8118450214540083
1212	54-55	0.08367467036151055
1212	56-57	-1.1030102916071272
1212	58-59	-0.19536261565204427
1212	60-61	0.3157573157202336
1212	62-63	-4.736617191134094
1212	64-65	-4.166147482859042
1212	66-67	-1.422920718036103
1212	68-69	0.40743249145674554
1212	70-71	-0.6977704142842072
1212	72-73	-1.0844030037776733
1212	74-75	0.09901815769139333
1212	76-77	1.158413161556762
1212	78-79	-1.0610772232408756
1212	80-81	0.8003781805652253
1212	82-83	0.7626726899605245
1212	84-85	-3.036765821669807
1212	86-87	-1.5430876820182533
1212	88-89	-0.5487802903143049
1212	90-91	0.2990160736757943
1212	92-93	-2.285120501563764
1212	94-95	-1.7494974203348441
1212	96-97	0.251271613428667
1212	98-99	-0.6340123952758177
1212	100-101	-0.05167611591322796
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	8.0
10	13.0
11	24.0
12	108.0
13	454.0
14	1389.0
15	3374.0
16	6292.0
17	10421.0
18	16023.0
19	22909.0
20	31962.0
21	43116.0
22	56303.0
23	71583.0
24	89612.0
25	111209.0
26	134703.0
27	161654.0
28	194473.0
29	228860.0
30	269055.0
31	314152.0
32	362001.0
33	411285.0
34	458894.0
35	501062.0
36	521171.0
37	470660.0
38	170844.0
39	476.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.785594083918404	32.36193891342574	16.895538515922272	19.95692848673358
2	23.4200915660243	27.907954554469523	26.72355255419379	21.948401325312393
3	21.59846297266678	26.06293475995633	30.623395713784618	21.715206553592274
4	24.194376263912687	25.027272212702677	28.42945930694275	22.348892216441886
5	22.345554578587766	28.20568895418207	26.914590645851465	22.5341658213787
6	22.185587716877738	27.485655833044426	27.278927448027922	23.04982900204992
7	23.132074395632504	28.033565382836656	27.905437522552628	20.928922698978212
8	21.3576664777767	26.56155293711036	29.036140161073188	23.044640424039752
9	22.708583516059186	26.734062435745788	27.544788470036284	23.012565578158746
10-11	22.82065251299771	27.112839350690198	27.176935441439714	22.889572694872378
12-13	22.767617105274184	26.98458201874956	27.23884885317312	23.008952022803136
14-15	22.517530481023044	27.313224303859457	27.257050436735735	22.912194778381767
16-17	22.616889276151824	27.37214391201508	27.231912371850175	22.779054439982914
18-19	23.059018960069256	27.289998628399516	26.76880117609912	22.88218123543211
20-21	22.758584980287154	27.193426028075567	27.024154012448943	23.023834979188333
22-23	22.65945282800014	27.010933105721996	27.30747534728632	23.02213871899155
24-25	22.83180152359806	26.853517652207042	27.216128501780943	23.098552322413948
26-27	22.78441822854657	27.147186879501277	27.15515198995903	22.913242901993122
28-29	23.022031516966543	27.05501457840338	27.091356064879523	22.831597839750554
30-31	23.155412276475737	26.948026957450015	26.78906779477502	23.10749297129923
32-33	22.759826084010797	26.980434129608533	27.138203349805995	23.121536436574672
34-35	23.030864963826822	26.981934957958583	27.03681167455781	22.950388403656792
36-37	23.06485872595539	27.088322247571927	26.950331800987588	22.896487225485092
38-39	22.919353417418314	26.968695507870667	27.195963800877816	22.915987273833206
40-41	23.094924268453685	27.01627302271705	26.94798411023138	22.94081859859788
42-43	22.924209669150965	26.83695493934402	27.16021192553919	23.078623465965823
44-45	22.83063302152552	26.822010977058554	27.284094585633085	23.06326141578284
46-47	22.949159230450846	27.051880874717725	27.16589570211388	22.833064192717547
48-49	23.102452848756894	26.843603045998073	27.06761258955933	22.986331515685706
50-51	22.952779713669546	26.981703705538052	27.033418012294586	23.03209856849782
52-53	22.892046349927156	26.971688980796877	27.259301663670364	22.876963005605603
54-55	22.908415715539242	26.752373106031165	27.23873856730809	23.100472611121504
56-57	22.856070822872066	26.981151493450945	27.160727954151238	23.00204972952575
58-59	22.914411283765826	26.920046428026094	27.28845111971613	22.87709116849195
60-61	23.130163734359517	26.791481079189662	26.973051990794204	23.10530319565662
62-63	22.871378845932636	26.842361479101406	27.19769761565872	23.08856205930724
64-65	22.961539051162305	26.901276921554622	27.16314495065974	22.974039076623335
66-67	23.010159087785485	26.87205329956476	27.07970127166024	23.038086340989512
68-69	22.933947071119526	26.84097214315717	27.16885357924012	23.056227206483186
70-71	23.113227825201346	26.983133886335313	26.994529836645537	22.909108451817804
72-73	22.951149669669917	26.779896446561363	27.195341371474445	23.073612512294275
74-75	22.832692163108387	26.76343441010391	27.312242803043425	23.09163062374428
76-77	22.900481403914903	27.0605800146778	27.18228656927637	22.856652012130926
78-79	23.15684087999518	26.853825767232102	26.964716017401962	23.024617335370753
80-81	22.93945674093933	26.843703279967627	27.09678700333889	23.12005297575415
82-83	22.99421950817094	26.897946375724143	27.102057698140165	23.005776417964753
84-85	22.989381521836783	26.652030693090325	27.127335328680097	23.231252456392795
86-87	22.81066152920031	26.894647096693614	27.227949875396074	23.066741498710005
88-89	22.969364645966067	26.880134535167105	27.16362884726965	22.98687197159718
90-91	23.10633179782501	26.74141301435249	26.91393922809692	23.238315959725583
92-93	22.824730936550086	26.732069155396367	27.21264375210139	23.23055615595216
94-95	23.00065166947684	26.796406575707415	27.036992482105894	23.165949272709845
96-97	22.972858081276623	26.802932874090313	27.04334715343768	23.180861891195384
98-99	22.85938579184494	26.78295618364875	27.166002685088753	23.19165533941756
100-101	23.054283168976475	26.728513178086626	26.943642200283673	23.273561452653226
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	4.5
2	8.5
3	14.0
4	18.0
5	27.0
6	46.0
7	70.5
8	108.5
9	179.5
10	284.0
11	411.5
12	629.5
13	909.0
14	1272.5
15	1891.5
16	2817.0
17	4074.5
18	5867.0
19	8393.0
20	11817.5
21	16118.0
22	21285.0
23	27405.5
24	34470.5
25	42447.0
26	51320.5
27	60948.0
28	70794.5
29	80357.5
30	90355.5
31	99820.0
32	108527.5
33	117076.0
34	125004.5
35	132592.0
36	139037.0
37	143776.5
38	146964.0
39	147873.5
40	146159.0
41	141887.5
42	136005.0
43	129753.5
44	123585.0
45	117987.0
46	112317.0
47	107093.0
48	101635.5
49	94776.5
50	88726.5
51	83213.5
52	78087.5
53	75015.0
54	72953.0
55	71780.5
56	71836.0
57	71991.5
58	72493.0
59	73222.0
60	73512.5
61	73790.0
62	73622.5
63	72960.0
64	72592.5
65	72439.5
66	71725.0
67	70015.0
68	67490.0
69	63976.5
70	58859.5
71	52505.5
72	45214.0
73	37040.5
74	28872.0
75	21508.5
76	15142.5
77	9986.5
78	6315.5
79	3929.0
80	2398.5
81	1474.5
82	961.0
83	669.5
84	464.5
85	325.0
86	237.5
87	162.5
88	100.0
89	83.0
90	67.0
91	41.5
92	30.0
93	22.5
94	10.5
95	4.5
96	3.5
97	2.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.7115641611623787
2	0.0028730142700903565
3	4.931293150155089E-4
4	6.43212150020229E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.19172010151602958
14-15	1.0720202500337151E-5
16-17	4.0736769501281174E-4
18-19	0.11363414650357379
20-21	1.0720202500337151E-5
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.001790273817556304
42-43	0.0
44-45	0.0
46-47	0.023605885905742405
48-49	8.576162000269721E-5
50-51	9.648182250303435E-5
52-53	1.286424300040458E-4
54-55	0.0023262839425731616
56-57	0.0013078647050411324
58-59	0.09445570423047063
60-61	0.001565149565049224
62-63	0.001211382882538098
64-65	0.002154760702567767
66-67	0.004030796140126768
68-69	0.004502485050141603
70-71	0.003291102167603505
72-73	0.03988987350375454
74-75	0.046579279863964915
76-77	0.1586268363974888
78-79	4.288081000134861E-4
80-81	0.11483480918361155
82-83	0.0045989668726446374
84-85	0.005145697200161833
86-87	0.006721566967711394
88-89	0.007053893245221845
90-91	0.02211577775819554
92-93	0.010870285335341871
94-95	0.01466523702046122
96-97	0.010173472172819956
98-99	0.01196374599037626
100-101	0.013089367252911661
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4664091.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.983807561156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.26273259613443	94.31631215940696
2	1.2443231133647419	2.388697405141998
3	0.10432599315422937	0.30040818151626086
4	0.0717856880978046	0.27561054688099373
5	0.04963994657364426	0.23823155396353676
6	0.054476461576058594	0.31373149227174707
7	0.03925669235927874	0.26376047634303595
8	0.03110486166664657	0.2388450445142226
9	0.032041802217083484	0.2767944760125447
>10	0.11028323697356268	1.364362107655346
>50	0.0	0.0
>100	0.0	0.0
>500	2.9607882513580974E-5	0.023246556293360813
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	6.432121500202291E-5	0.0	0.0
2	1.2864243000404582E-4	0.0	0.0
3	1.2864243000404582E-4	0.0	0.0
4	1.2864243000404582E-4	0.0	0.0
5	1.929636450060687E-4	2.1440405000674302E-5	0.0
6	2.5728486000809164E-4	4.2880810001348604E-5	0.0
7	2.787252650087659E-4	4.2880810001348604E-5	0.0
8	3.001656700094402E-4	4.2880810001348604E-5	0.0
9	3.216060750101145E-4	4.2880810001348604E-5	0.0
10-11	3.4304648001078883E-4	4.2880810001348604E-5	0.0
12-13	3.644868850114631E-4	4.2880810001348604E-5	0.0
14-15	4.0736769501281174E-4	4.2880810001348604E-5	0.0
16-17	4.180878975131489E-4	7.504141750236005E-5	1.0720202500337151E-5
18-19	4.28808100013486E-4	9.648182250303436E-5	3.2160607501011454E-5
20-21	4.3952830251382314E-4	1.2864243000404582E-4	4.2880810001348604E-5
22-23	4.502485050141603E-4	1.3936263250438296E-4	4.2880810001348604E-5
24-25	4.502485050141603E-4	1.500828350047201E-4	4.2880810001348604E-5
26-27	4.502485050141603E-4	1.6080303750505727E-4	4.2880810001348604E-5
28-29	4.931293150155089E-4	1.7152324000539441E-4	5.360101250168575E-5
30-31	5.574505300175318E-4	1.7152324000539441E-4	6.432121500202291E-5
32-33	6.43212150020229E-4	1.929636450060687E-4	6.432121500202291E-5
34-35	6.646525550209033E-4	1.929636450060687E-4	6.432121500202291E-5
36-37	8.361757950262978E-4	1.929636450060687E-4	6.432121500202291E-5
38-39	0.0010613000475333779	1.929636450060687E-4	9.648182250303436E-5
40-41	0.0013185849075414693	2.0368384750640584E-4	1.072020250033715E-4
42-43	0.0017902738175563041	2.14404050006743E-4	1.2864243000404582E-4
44-45	0.0023906051575751843	2.14404050006743E-4	1.3936263250438296E-4
46-47	0.0031731799400997966	2.4656465750775447E-4	1.8224344250573156E-4
48-49	0.004105837557629128	2.5728486000809164E-4	2.14404050006743E-4
50-51	0.005349381047668238	2.5728486000809164E-4	2.358444550074173E-4
52-53	0.006796608385213753	2.5728486000809164E-4	2.358444550074173E-4
54-55	0.008908488277780173	2.5728486000809164E-4	2.5728486000809164E-4
56-57	0.011288373232855019	2.787252650087659E-4	2.5728486000809164E-4
58-59	0.013678978390430203	2.787252650087659E-4	2.787252650087659E-4
60-61	0.01712016339303843	3.001656700094402E-4	3.001656700094402E-4
62-63	0.02140824439317329	3.001656700094402E-4	3.108858725097773E-4
64-65	0.026178734505823323	3.3232627751045166E-4	3.7520708751180023E-4
66-67	0.03178540041349965	3.4304648001078883E-4	3.9664749251247457E-4
68-69	0.038796412848720145	3.4304648001078883E-4	4.0736769501281174E-4
70-71	0.04781210315150369	3.7520708751180023E-4	4.3952830251382314E-4
72-73	0.05749244600930814	3.9664749251247457E-4	4.502485050141603E-4
74-75	0.06883442025466485	4.28808100013486E-4	4.502485050141603E-4
76-77	0.0817201036600701	4.28808100013486E-4	4.502485050141603E-4
78-79	0.09833641753559269	4.28808100013486E-4	4.8240911251517176E-4
80-81	0.11904784876624405	4.28808100013486E-4	4.931293150155089E-4
82-83	0.14243933062197972	4.28808100013486E-4	4.931293150155089E-4
84-85	0.16733164082776258	4.3952830251382314E-4	5.038495175158462E-4
86-87	0.19646915122367895	4.502485050141603E-4	5.145697200161833E-4
88-89	0.2302163486947403	4.609687075144975E-4	5.145697200161833E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTAT	925	0.0	7.9545226	42-43
CGTATGC	1100	0.0	7.120581	44-45
TCGGAAG	2785	0.0	6.8148875	3
TCGTATG	1275	0.0	6.5155644	42-43
GATCGGA	3090	0.0	6.341392	1
CGGAAGA	3195	0.0	6.237382	4
TATGCCG	1230	3.092282E-11	5.9820595	46-47
AGAGCAC	3285	0.0	5.94487	8
ATCGGAA	3020	0.0	5.813433	2
CGTCTGA	1840	0.0	5.5569015	16-17
GAGCACA	3580	0.0	5.4549994	9
TCTCGTA	1240	8.052666E-9	5.358399	40-41
GCCGTCT	1930	0.0	5.286071	48-49
ACACGTC	1960	0.0	5.2208056	12-13
>>END_MODULE