FastQCFastQC Report
Thu 11 Sep 2014
NYCLib28_CAAAAG_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNYCLib28_CAAAAG_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4664091
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT9250.07.954522642-43
CGTATGC11000.07.12058144-45
TCGGAAG27850.06.81488753
TCGTATG12750.06.515564442-43
GATCGGA30900.06.3413921
CGGAAGA31950.06.2373824
TATGCCG12303.092282E-115.982059546-47
AGAGCAC32850.05.944878
ATCGGAA30200.05.8134332
CGTCTGA18400.05.556901516-17
GAGCACA35800.05.45499949
TCTCGTA12408.052666E-95.35839940-41
GCCGTCT19300.05.28607148-49
ACACGTC19600.05.220805612-13
Produced by FastQC (version 0.11.2)