##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	NYCLib27_ATTCCT_L007_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2652039
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.890739163338097	27.0	22.0	33.0	2.0	33.0
2	24.91581270109527	33.0	15.0	33.0	2.0	33.0
3	25.79536613149354	33.0	22.0	33.0	2.0	33.0
4	27.92435518482194	33.0	22.0	37.0	2.0	37.0
5	28.00411720943772	33.0	15.0	37.0	6.0	37.0
6	29.05139554885882	33.0	27.0	37.0	15.0	37.0
7	28.704330517009744	33.0	27.0	37.0	2.0	37.0
8	28.466827976511656	33.0	27.0	37.0	2.0	37.0
9	29.24348699246127	33.0	27.0	37.0	15.0	37.0
10-11	28.862392672204294	33.0	24.5	37.0	10.5	37.0
12-13	29.157942435989817	33.0	27.0	37.0	10.5	37.0
14-15	29.510815451808966	33.0	27.0	37.0	6.0	40.0
16-17	30.392355466869077	33.0	27.0	37.0	15.0	40.0
18-19	30.715342798503343	33.0	27.0	37.0	15.0	40.0
20-21	30.721680563521126	33.0	27.0	37.0	15.0	40.0
22-23	30.740713277595088	33.0	27.0	37.0	15.0	40.0
24-25	30.32402163014948	33.0	27.0	37.0	10.5	40.0
26-27	29.79096065329356	33.0	27.0	37.0	10.5	40.0
28-29	30.00235479191671	33.0	27.0	37.0	15.0	40.0
30-31	29.909359364624727	33.0	27.0	37.0	15.0	40.0
32-33	29.720650601292064	33.0	27.0	37.0	15.0	40.0
34-35	29.764859792785852	33.0	27.0	37.0	15.0	40.0
36-37	30.002901163972325	33.0	27.0	37.0	15.0	40.0
38-39	30.048148047596584	33.0	27.0	37.0	15.0	40.0
40-41	30.124394286811018	33.0	27.0	37.0	15.0	40.0
42-43	30.02765683310087	33.0	27.0	37.0	15.0	40.0
44-45	29.761018785922833	33.0	27.0	37.0	15.0	40.0
46-47	29.370594663200656	33.0	27.0	37.0	15.0	40.0
48-49	29.205185896587494	33.0	22.0	37.0	10.5	40.0
50-51	28.41790957825281	33.0	22.0	37.0	10.5	38.5
52-53	28.122767236831734	33.0	22.0	37.0	10.5	37.0
54-55	29.060573392774387	33.0	22.0	37.0	15.0	40.0
56-57	28.962742440816292	33.0	22.0	37.0	10.5	40.0
58-59	29.158511809215476	33.0	22.0	37.0	15.0	40.0
60-61	28.910607837969202	33.0	24.5	37.0	10.5	40.0
62-63	28.778922557322876	33.0	22.0	37.0	15.0	40.0
64-65	28.74065049571292	33.0	22.0	37.0	10.5	40.0
66-67	28.618001846880833	33.0	22.0	37.0	15.0	40.0
68-69	28.3239609221433	33.0	22.0	37.0	15.0	38.5
70-71	28.231762051764697	33.0	22.0	37.0	10.5	37.0
72-73	28.004301595866426	33.0	22.0	37.0	15.0	37.0
74-75	27.951979024441194	33.0	22.0	37.0	15.0	37.0
76-77	27.566663046810397	33.0	22.0	37.0	6.0	37.0
78-79	27.000674009695935	27.0	22.0	33.0	6.0	37.0
80-81	26.898688895600706	27.0	22.0	33.0	6.0	37.0
82-83	26.483579238465197	27.0	22.0	33.0	6.0	37.0
84-85	26.59160329090183	27.0	22.0	33.0	6.0	37.0
86-87	26.2975623661643	27.0	22.0	33.0	6.0	37.0
88-89	25.945421428568736	27.0	22.0	33.0	6.0	37.0
90-91	25.832375956763833	27.0	22.0	33.0	6.0	37.0
92-93	25.722594200160707	27.0	22.0	33.0	6.0	37.0
94-95	25.51774672242754	27.0	22.0	33.0	6.0	37.0
96-97	25.400187365268764	27.0	22.0	33.0	6.0	37.0
98-99	25.179062600512285	27.0	15.0	33.0	6.0	37.0
100-101	24.70910288272533	27.0	18.5	33.0	6.0	35.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
2306	1	8.32728217872538
2306	2	8.572953203755024
2306	3	7.52758689954349
2306	4	9.229559465747819
2306	5	8.887401907022216
2306	6	8.001912059261663
2306	7	8.259249377106336
2306	8	8.693034135946885
2306	9	7.580550906671462
2306	10-11	7.8726963415086
2306	12-13	7.466337678880684
2306	14-15	8.106460421746466
2306	16-17	6.880604705778676
2306	18-19	6.3347940972207795
2306	20-21	6.263171755519828
2306	22-23	5.962776416746376
2306	24-25	5.980453771798167
2306	26-27	6.647785611360934
2306	28-29	5.585801721257855
2306	30-31	5.521527399201016
2306	32-33	5.524472344807442
2306	34-35	4.871978814887516
2306	36-37	4.972662598388325
2306	38-39	4.728729051737357
2306	40-41	3.84573833472189
2306	42-43	3.714926167422089
2306	44-45	3.3424800480503976
2306	46-47	3.586234197862886
2306	48-49	3.4979255135694984
2306	50-51	3.4953791777226257
2306	52-53	3.968579126703002
2306	54-55	3.5661737925682253
2306	56-57	3.8087027810875718
2306	58-59	3.350730902138025
2306	60-61	3.3229675026864705
2306	62-63	2.739197416361378
2306	64-65	2.414040871474686
2306	66-67	2.2729931411199473
2306	68-69	2.401210579526598
2306	70-71	2.5094526903184864
2306	72-73	1.7768825182399013
2306	74-75	1.5152362254608924
2306	76-77	1.8166507314255647
2306	78-79	2.0985562530339905
2306	80-81	1.8119887212855659
2306	82-83	1.7991684274136368
2306	84-85	1.3188310219507642
2306	86-87	1.216072165338968
2306	88-89	1.352837935155435
2306	90-91	1.129043820660815
2306	92-93	1.1217644604083326
2306	94-95	1.2776569634533388
2306	96-97	1.0774195042724948
2306	98-99	0.8558005796791939
2306	100-101	0.685640114201874
2307	1	8.259554612204198
2307	2	8.43139112970833
2307	3	7.554181931320752
2307	4	9.1742256385089
2307	5	9.129845486092776
2307	6	7.610752806050861
2307	7	8.23963892665553
2307	8	8.626541849495187
2307	9	7.6916858246481254
2307	10-11	7.863780694446049
2307	12-13	7.42292318949449
2307	14-15	8.286712031171785
2307	16-17	6.860252746647401
2307	18-19	6.402217922339062
2307	20-21	6.403095991882019
2307	22-23	6.0184999037775775
2307	24-25	6.261600062774534
2307	26-27	7.083403411805573
2307	28-29	6.230903527659091
2307	30-31	6.050750319780899
2307	32-33	6.266429894279334
2307	34-35	5.883868672002553
2307	36-37	5.65914411941602
2307	38-39	5.276549657237023
2307	40-41	4.666566039257514
2307	42-43	4.4000631717183545
2307	44-45	4.430892176500201
2307	46-47	4.709020463877245
2307	48-49	4.455808145674169
2307	50-51	4.396908946207169
2307	52-53	4.925350130613783
2307	54-55	4.4868039668712285
2307	56-57	4.854763290549403
2307	58-59	4.434945106700653
2307	60-61	4.406310368744332
2307	62-63	3.9960788151059425
2307	64-65	3.8484823378416237
2307	66-67	3.580226850163399
2307	68-69	3.622700739772988
2307	70-71	3.3872946086573386
2307	72-73	2.9294990482877097
2307	74-75	2.6297873632687256
2307	76-77	2.8019478483332882
2307	78-79	3.2012952303348143
2307	80-81	2.9621872269837723
2307	82-83	3.008741727180219
2307	84-85	2.37664514850092
2307	86-87	2.389261047805956
2307	88-89	2.472321878558361
2307	90-91	2.23549332969505
2307	92-93	2.1621999553155966
2307	94-95	2.3538718237765757
2307	96-97	2.0600562633088373
2307	98-99	2.0418884151836174
2307	100-101	1.6852450970906574
1101	1	7.91727702405538
1101	2	7.8068179212470135
1101	3	7.161206892243481
1101	4	8.596045946759311
1101	5	8.48341617803851
1101	6	7.231166281996856
1101	7	6.985436729835499
1101	8	7.904262572884729
1101	9	7.133794718291213
1101	10-11	7.411045930031946
1101	12-13	7.154321485968133
1101	14-15	7.55813603118796
1101	16-17	6.704149286413195
1101	18-19	5.917611043222806
1101	20-21	5.686025480409576
1101	22-23	5.272167758096703
1101	24-25	5.8701611320055065
1101	26-27	6.352693643701112
1101	28-29	5.850390877340505
1101	30-31	5.487808425610037
1101	32-33	5.568236472404557
1101	34-35	5.296407938846219
1101	36-37	5.199605777182029
1101	38-39	4.906335485483066
1101	40-41	4.412673414801713
1101	42-43	4.129927318802569
1101	44-45	3.970644895226755
1101	46-47	4.421053520821879
1101	48-49	4.093654166566605
1101	50-51	3.5958580684093278
1101	52-53	4.5842803107028764
1101	54-55	4.313963883398539
1101	56-57	4.444409292434006
1101	58-59	3.8920033313287377
1101	60-61	4.073912998396953
1101	62-63	3.8277381785862374
1101	64-65	3.623358837488766
1101	66-67	3.2317931384596292
1101	68-69	3.4543007669703663
1101	70-71	3.348254999571598
1101	72-73	2.8167525910239846
1101	74-75	2.2902582646039242
1101	76-77	2.4459585235561754
1101	78-79	3.001285195615189
1101	80-81	2.6526776745373226
1101	82-83	2.88191197062201
1101	84-85	2.1291967350482253
1101	86-87	2.195816487483391
1101	88-89	2.270174094689544
1101	90-91	2.143417131038941
1101	92-93	1.9093487805709408
1101	94-95	2.1932517286565307
1101	96-97	2.0865774332813167
1101	98-99	1.868653893928208
1101	100-101	1.6410074021340044
2201	1	7.188627654544899
2201	2	7.7965297785676775
2201	3	6.264105350088094
2201	4	8.425436066075072
2201	5	4.008619484636036
2201	6	5.765997872654147
2201	7	6.6478437623728865
2201	8	7.740637757103936
2201	9	6.532826698568499
2201	10-11	4.2445521705876
2201	12-13	6.415333542943181
2201	14-15	7.071997099188788
2201	16-17	6.085423967244989
2201	18-19	2.0783047204639438
2201	20-21	5.121364381929482
2201	22-23	5.19904601181597
2201	24-25	5.483270903564858
2201	26-27	6.156171365446038
2201	28-29	5.108549949377821
2201	30-31	5.215628193690012
2201	32-33	5.395218811078237
2201	34-35	4.914191819347284
2201	36-37	4.95074133829462
2201	38-39	4.467632919799353
2201	40-41	4.241677055409692
2201	42-43	4.053534790665378
2201	44-45	3.9653257194488987
2201	46-47	4.290915252655239
2201	48-49	4.027087987307603
2201	50-51	3.650216989362292
2201	52-53	4.51203348737241
2201	54-55	4.221269516841367
2201	56-57	4.36960090437633
2201	58-59	3.987724998787243
2201	60-61	3.963530938931587
2201	62-63	3.7216871469965405
2201	64-65	3.570908151213093
2201	66-67	3.2517217707854726
2201	68-69	3.501271933274417
2201	70-71	3.249837032846994
2201	72-73	2.715099544572613
2201	74-75	2.361833370656914
2201	76-77	2.610038415334568
2201	78-79	2.8817722534039447
2201	80-81	2.4946241887100555
2201	82-83	2.877104704090602
2201	84-85	2.3223004082296725
2201	86-87	2.255179383609871
2201	88-89	2.2483792691518474
2201	90-91	2.211454225425534
2201	92-93	2.2004369171443265
2201	94-95	2.1458833603902043
2201	96-97	2.1031939361429117
2201	98-99	2.091537842578706
2201	100-101	1.6688342475619464
2304	1	1.5327926697294387
2304	2	4.862178453358357
2304	3	5.672298911512602
2304	4	4.324053992050395
2304	5	6.446410688229442
2304	6	6.303157172182516
2304	7	6.890133876099121
2304	8	6.41621137683023
2304	9	5.776911103871562
2304	10-11	6.600751723981574
2304	12-13	6.398858759623877
2304	14-15	6.70570259363571
2304	16-17	5.427178180705408
2304	18-19	5.106037922059393
2304	20-21	4.860818477599864
2304	22-23	4.474289102476863
2304	24-25	4.751668578345701
2304	26-27	5.673669792744395
2304	28-29	4.936866406768619
2304	30-31	4.822785247181539
2304	32-33	5.03827914413251
2304	34-35	4.656264625836929
2304	36-37	4.672394997193322
2304	38-39	4.255017417830739
2304	40-41	3.669582405577305
2304	42-43	3.1395313537214378
2304	44-45	3.1246043894905426
2304	46-47	4.0592749779788875
2304	48-49	3.707258210252135
2304	50-51	3.277463779639234
2304	52-53	3.9562151099056138
2304	54-55	3.72864273551685
2304	56-57	4.311157828244884
2304	58-59	3.6839519662028906
2304	60-61	3.55245385323418
2304	62-63	3.2722980420517374
2304	64-65	3.112901148844287
2304	66-67	3.024068409772166
2304	68-69	3.2329818414375247
2304	70-71	3.147386505139366
2304	72-73	2.8200599708627934
2304	74-75	2.289939690363763
2304	76-77	2.5407569028508235
2304	78-79	2.91837174887047
2304	80-81	2.7009240603243043
2304	82-83	2.703932448292381
2304	84-85	2.2719383458222033
2304	86-87	2.1007039172796453
2304	88-89	2.365729459604985
2304	90-91	2.277570309047899
2304	92-93	2.160696420053231
2304	94-95	2.398160010584874
2304	96-97	2.3480140136819436
2304	98-99	2.188382359092312
2304	100-101	1.8027754093276762
1102	1	6.880375553168786
1102	2	7.358603840612506
1102	3	5.953795008725891
1102	4	5.833845816225782
1102	5	6.402425709017933
1102	6	5.656122552135237
1102	7	6.432966609908881
1102	8	6.248953701373349
1102	9	4.636726361442001
1102	10-11	5.815625135752626
1102	12-13	5.569338083422316
1102	14-15	6.019221462929163
1102	16-17	4.951381409795321
1102	18-19	5.0706066198686415
1102	20-21	5.345476167319006
1102	22-23	4.974209453768463
1102	24-25	1.57840274714491
1102	26-27	5.416246098513124
1102	28-29	4.728894803927947
1102	30-31	4.59792718685642
1102	32-33	4.807591295048546
1102	34-35	4.731623302242145
1102	36-37	4.681447232554717
1102	38-39	3.772605103253465
1102	40-41	3.7858276111330618
1102	42-43	3.8973087219223395
1102	44-45	-3.9906304393818175
1102	46-47	1.255931319878684
1102	48-49	-0.6811041023206172
1102	50-51	2.5948093347623526
1102	52-53	-0.4395778595082156
1102	54-55	2.52542118474053
1102	56-57	-0.9610891292276769
1102	58-59	2.76539458879893
1102	60-61	3.7484841367729373
1102	62-63	3.408456843837147
1102	64-65	2.8716230815441435
1102	66-67	2.974761335796078
1102	68-69	3.3826914784345448
1102	70-71	3.2039225420112167
1102	72-73	2.8417777256419825
1102	74-75	2.4570955875434883
1102	76-77	2.768982560236495
1102	78-79	-1.3722651019792522
1102	80-81	1.503565160051231
1102	82-83	2.5990197993324458
1102	84-85	2.2729387442431346
1102	86-87	2.3343979285837335
1102	88-89	2.374575876672587
1102	90-91	2.211568981096381
1102	92-93	2.084303463482531
1102	94-95	2.4104032942829328
1102	96-97	2.404365208785091
1102	98-99	2.4040028101133224
1102	100-101	2.019749977087084
2202	1	-20.71698550240898
2202	2	-7.744888031544164
2202	3	-0.661093947506064
2202	4	1.6390290823732983
2202	5	1.250279754159962
2202	6	1.84022600667031
2202	7	3.9196487188966422
2202	8	2.801708004339286
2202	9	3.427147037819914
2202	10-11	2.123046308380456
2202	12-13	1.538098779192321
2202	14-15	2.868776567198161
2202	16-17	1.7846721089783202
2202	18-19	1.7146678405120603
2202	20-21	1.3303024349274502
2202	22-23	1.582735915133668
2202	24-25	2.4592671928621535
2202	26-27	3.579902984152202
2202	28-29	-1.0657417990293219
2202	30-31	0.9872744921478862
2202	32-33	1.7960671296482609
2202	34-35	1.8338685119137743
2202	36-37	3.1080074371754556
2202	38-39	2.480184139771257
2202	40-41	1.955412857488735
2202	42-43	0.7938360254730128
2202	44-45	0.9270219005036857
2202	46-47	-1.1924074890770733
2202	48-49	0.8162579579289755
2202	50-51	1.8264811985126244
2202	52-53	1.6998686571255242
2202	54-55	-0.6088692401863831
2202	56-57	1.1240957627493344
2202	58-59	2.0767115937679
2202	60-61	2.315763096172507
2202	62-63	-2.0304154448893073
2202	64-65	0.10932432866066222
2202	66-67	0.7506173620736547
2202	68-69	1.5184875103479456
2202	70-71	-2.4438253170476223
2202	72-73	-4.613701232810879
2202	74-75	-1.1130720931493947
2202	76-77	-0.08299351331089255
2202	78-79	1.6544763154909674
2202	80-81	1.5978802487053088
2202	82-83	-0.5916932123944889
2202	84-85	-0.12627900282087978
2202	86-87	0.706882179628419
2202	88-89	1.342931032798102
2202	90-91	1.4329688724565806
2202	92-93	1.4015819157160365
2202	94-95	1.6574931271103672
2202	96-97	1.2101880596399148
2202	98-99	1.3113929828193065
2202	100-101	1.0700436552186368
2305	1	5.719618185275973
2305	2	7.040144791270713
2305	3	6.684818829564048
2305	4	7.679417580690952
2305	5	7.8583148307195785
2305	6	6.08145358066168
2305	7	3.184142897873091
2305	8	6.051177951668677
2305	9	6.4068604202701
2305	10-11	4.73997135446978
2305	12-13	5.23228287591278
2305	14-15	5.623983996795655
2305	16-17	5.051627675959924
2305	18-19	4.3433699251998235
2305	20-21	5.186214399683699
2305	22-23	5.12747691983007
2305	24-25	5.282979987498486
2305	26-27	6.068706307236372
2305	28-29	5.072892761312264
2305	30-31	5.386756453692822
2305	32-33	5.267767616052119
2305	34-35	5.25434273711787
2305	36-37	4.647874888671879
2305	38-39	4.60903436036919
2305	40-41	4.104963717218741
2305	42-43	4.024588517948384
2305	44-45	3.224850839083352
2305	46-47	4.0044503961999
2305	48-49	3.512553740711077
2305	50-51	2.362816404640977
2305	52-53	3.8312145292044057
2305	54-55	4.244271727837656
2305	56-57	4.731364255910417
2305	58-59	4.3599712129637425
2305	60-61	4.517341173971712
2305	62-63	3.886606171404093
2305	64-65	4.000741613962724
2305	66-67	3.6149748611303103
2305	68-69	4.050092899382214
2305	70-71	3.6420812189001275
2305	72-73	-0.8993916613721638
2305	74-75	2.031441128179953
2305	76-77	2.995045265134049
2305	78-79	3.622092766073166
2305	80-81	3.214981094474183
2305	82-83	3.354976897485674
2305	84-85	2.883993935187476
2305	86-87	2.8738483446385708
2305	88-89	2.9187556097417975
2305	90-91	2.8290268238186123
2305	92-93	2.8771783463088205
2305	94-95	3.046907786499247
2305	96-97	2.966276137561767
2305	98-99	3.0110774398562334
2305	100-101	2.6060971363795247
1103	1	-20.71698550240898
1103	2	-7.81149797373422
1103	3	-0.6339812817241111
1103	4	3.0626914760294923
1103	5	4.803459884719633
1103	6	4.774408281321822
1103	7	4.104399872352694
1103	8	4.349377106890369
1103	9	4.450528376867716
1103	10-11	4.962634303100181
1103	12-13	4.592129981155971
1103	14-15	4.07976845186306
1103	16-17	2.8506229209203404
1103	18-19	3.128361199196334
1103	20-21	2.436612128393513
1103	22-23	2.722031387796786
1103	24-25	3.141925405714332
1103	26-27	4.276632427073487
1103	28-29	3.723751450641249
1103	30-31	3.6219595545856613
1103	32-33	3.046457197962738
1103	34-35	2.7166856058231303
1103	36-37	2.897710485707563
1103	38-39	2.4440098263883634
1103	40-41	2.79248489990837
1103	42-43	2.3808307720394914
1103	44-45	2.7584750391996984
1103	46-47	2.1912551613565867
1103	48-49	-0.846939265839751
1103	50-51	-1.5281755775529149
1103	52-53	1.8908991284405623
1103	54-55	2.5515427650938953
1103	56-57	3.434030745527739
1103	58-59	2.9970940346244674
1103	60-61	3.2641688290795905
1103	62-63	2.972031848617842
1103	64-65	2.8781924827691583
1103	66-67	2.6098960741712602
1103	68-69	3.028276626018023
1103	70-71	2.943164381507305
1103	72-73	2.2806485541221804
1103	74-75	2.103956746461563
1103	76-77	2.3565066810229602
1103	78-79	2.80096319124452
1103	80-81	2.557785935509166
1103	82-83	2.6243726533599165
1103	84-85	2.1330814987355424
1103	86-87	1.9976254395117259
1103	88-89	2.2290194132676078
1103	90-91	-2.3944695213770295
1103	92-93	-2.889227172572223
1103	94-95	-3.0885944013248157
1103	96-97	0.3614395288155272
1103	98-99	-1.0977484700509414
1103	100-101	0.9691463055122362
2203	1	2.5495930973268166
2203	2	3.1512458855118517
2203	3	-5.931962762141982
2203	4	-3.2779214187560513
2203	5	-0.043424907142473046
2203	6	0.29724729114319715
2203	7	2.3482087032960948
2203	8	-7.691800564951826
2203	9	-6.8235148525083495
2203	10-11	-12.257160175486156
2203	12-13	-10.142606200951128
2203	14-15	-1.5966835357054627
2203	16-17	-1.464171206674603
2203	18-19	-0.6694639305109433
2203	20-21	-1.3981610673511966
2203	22-23	-3.065711354658493
2203	24-25	-0.9916100667519885
2203	26-27	-1.4835850918640965
2203	28-29	0.3590331052784812
2203	30-31	-2.2351931713411197
2203	32-33	-0.21586642886321528
2203	34-35	-0.27007340348771436
2203	36-37	-0.633921177544913
2203	38-39	0.32103664213064675
2203	40-41	-0.8364768356483339
2203	42-43	0.11840120963002931
2203	44-45	-0.303219036703787
2203	46-47	0.06898380033960905
2203	48-49	0.1344411194849826
2203	50-51	0.02985893447332444
2203	52-53	0.9532061629462092
2203	54-55	-0.0038278818151482596
2203	56-57	0.2962718893767722
2203	58-59	0.3292373476002979
2203	60-61	0.08696364589399153
2203	62-63	0.5797152097114058
2203	64-65	0.151487621857477
2203	66-67	-0.6936387282205878
2203	68-69	0.1376324610903623
2203	70-71	0.28784638330693113
2203	72-73	-0.05908399043777024
2203	74-75	-0.5509284155369194
2203	76-77	-0.4430740438061669
2203	78-79	0.24660164458500233
2203	80-81	0.16858610015603404
2203	82-83	-0.25959023089588484
2203	84-85	-0.2168672884296612
2203	86-87	-0.14851860097353864
2203	88-89	-0.013921511690430322
2203	90-91	-0.5419023384339319
2203	92-93	-0.2294174418145296
2203	94-95	0.08933940808047325
2203	96-97	-0.15470049122288643
2203	98-99	-0.4652654931334119
2203	100-101	-0.591087167579488
2310	1	-20.71698550240898
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2310	3	-21.414657610226527
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2310	5	-23.583979728806938
2310	6	-24.645156936069483
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2310	8	-23.848207434172433
2310	9	-24.774109304291706
2310	10-11	-24.482655551972286
2310	12-13	-24.772725091307798
2310	14-15	-25.124820920117617
2310	16-17	-26.178966451060337
2310	18-19	-20.30886903331865
2310	20-21	-20.44022559977612
2310	22-23	-26.734007259546207
2310	24-25	-26.335731599116585
2310	26-27	-19.28948202423692
2310	28-29	-19.750201180384256
2310	30-31	-20.24328489851856
2310	32-33	-18.499207965614243
2310	34-35	-10.748305084917714
2310	36-37	-15.279160799093912
2310	38-39	-20.189497166380722
2310	40-41	-12.292110441132163
2310	42-43	-5.456790696782225
2310	44-45	-14.406919877511813
2310	46-47	-19.662154625427355
2310	48-49	-20.050935597806074
2310	50-51	-11.785691644530097
2310	52-53	-15.394500798569895
2310	54-55	-19.434238655975342
2310	56-57	-18.841106264499047
2310	58-59	-11.418984540471257
2310	60-61	-8.306909838799445
2310	62-63	-5.478859335111675
2310	64-65	-15.912881298522013
2310	66-67	-11.527576013515159
2310	68-69	-6.323331618088687
2310	70-71	-3.9445441489905697
2310	72-73	-4.834862032453927
2310	74-75	-4.603006022687367
2310	76-77	-3.7745534611210765
2310	78-79	-4.265231517632365
2310	80-81	-3.650152140907174
2310	82-83	-13.261467375835899
2310	84-85	-11.254511052620021
2310	86-87	-6.9026202816311155
2310	88-89	-5.016432039409807
2310	90-91	-2.68481557220991
2310	92-93	-2.2674204649571905
2310	94-95	-2.72063217031911
2310	96-97	-1.7293014696566367
2310	98-99	-1.7218019256108228
2310	100-101	-1.9145103776698598
2204	1	-20.71698550240898
2204	2	-20.5100486983719
2204	3	-21.414657610226527
2204	4	-23.39866118097534
2204	5	-23.583979728806938
2204	6	-24.645156936069483
2204	7	-24.210575973057935
2204	8	-23.848207434172433
2204	9	-24.774109304291706
2204	10-11	-18.483921374757095
2204	12-13	-18.992345344472355
2204	14-15	-19.047816700708335
2204	16-17	-20.31694113460464
2204	18-19	-26.59482720660077
2204	20-21	-14.198621467888513
2204	22-23	-6.982741436761398
2204	24-25	-16.253453118103927
2204	26-27	-25.460203321718783
2204	28-29	-13.366641749383213
2204	30-31	-15.862980517572355
2204	32-33	-19.470695618034327
2204	34-35	-19.75526143753283
2204	36-37	-26.0213716398963
2204	38-39	-13.675144151475024
2204	40-41	-7.788694394410715
2204	42-43	-5.35086677581106
2204	44-45	-14.535886851584866
2204	46-47	-20.633465073462713
2204	48-49	-18.966240005765144
2204	50-51	-11.094343864717352
2204	52-53	-3.493684739962788
2204	54-55	-25.50316737983364
2204	56-57	-19.288113509349046
2204	58-59	-11.899054990019332
2204	60-61	-15.946317014496513
2204	62-63	-12.719295430780434
2204	64-65	-6.130526100976944
2204	66-67	-4.036780376632876
2204	68-69	-7.502041969958249
2204	70-71	-7.8049965064031674
2204	72-73	-4.115077000821863
2204	74-75	-3.9252794793074095
2204	76-77	-3.8129484648218366
2204	78-79	-17.9698158707013
2204	80-81	-7.996373851685846
2204	82-83	-4.491878187464398
2204	84-85	-3.2056590993198597
2204	86-87	-2.785220215719729
2204	88-89	-3.7931569458102388
2204	90-91	-2.979703117659053
2204	92-93	-2.5543727116170523
2204	94-95	-1.9535243835910698
2204	96-97	-2.146493266390241
2204	98-99	-2.3173442628718313
2204	100-101	-2.6694567031100327
2311	1	8.29642577682073
2311	2	8.472757353897702
2311	3	7.48905628248324
2311	4	9.102714334843093
2311	5	8.842774053861561
2311	6	7.864299735182236
2311	7	8.15135662666696
2311	8	8.290719663489188
2311	9	7.569941589793579
2311	10-11	7.784538395758112
2311	12-13	7.389843684483125
2311	14-15	8.087954183046069
2311	16-17	6.9026188309203995
2311	18-19	6.2015964522713105
2311	20-21	6.311786891997492
2311	22-23	5.80940745847305
2311	24-25	5.833026997857274
2311	26-27	6.595505068927711
2311	28-29	5.635073872878085
2311	30-31	5.1810086379073645
2311	32-33	5.306410749385101
2311	34-35	5.067166870234477
2311	36-37	5.261124921314149
2311	38-39	4.529479279305189
2311	40-41	3.569156782903139
2311	42-43	3.6178364668078835
2311	44-45	3.2925174775174497
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2311	54-55	3.3315987824772257
2311	56-57	3.651244960520195
2311	58-59	3.1743392983978964
2311	60-61	3.1303013204909185
2311	62-63	2.8237833885975085
2311	64-65	2.5238606046916914
2311	66-67	2.117803878063903
2311	68-69	2.2769428906619495
2311	70-71	2.0754902491786957
2311	72-73	1.577807316556644
2311	74-75	1.198737926481364
2311	76-77	1.3669994745161738
2311	78-79	1.7767512887134416
2311	80-81	1.5937544282229155
2311	82-83	1.5155577233650313
2311	84-85	1.0750454462665928
2311	86-87	0.9696039562139447
2311	88-89	0.8345380059886693
2311	90-91	0.844466131727188
2311	92-93	0.6251854256907698
2311	94-95	0.9330139886571764
2311	96-97	0.7683337204175729
2311	98-99	0.4894197781266669
2311	100-101	0.40006401378327183
2205	1	8.393302279772648
2205	2	8.553539101042198
2205	3	7.677019108711953
2205	4	9.341645556918856
2205	5	9.225429197610431
2205	6	7.919550973632397
2205	7	8.222648221324278
2205	8	8.555549243408127
2205	9	7.640676151507016
2205	10-11	7.932819202465069
2205	12-13	7.588122746017724
2205	14-15	7.994341582036437
2205	16-17	7.014381817076835
2205	18-19	6.415770759968247
2205	20-21	6.4683734154506
2205	22-23	6.089963100612334
2205	24-25	6.082069538226172
2205	26-27	6.66163021265999
2205	28-29	5.907361294291999
2205	30-31	5.8344238513303885
2205	32-33	5.829957361546292
2205	34-35	5.587383462420707
2205	36-37	5.53971745195274
2205	38-39	4.952543622417576
2205	40-41	4.453313485988705
2205	42-43	4.296391480685113
2205	44-45	4.2645049870610805
2205	46-47	4.490972199270509
2205	48-49	4.10642741273147
2205	50-51	3.9526497243690457
2205	52-53	4.690352143331246
2205	54-55	4.308654091818951
2205	56-57	4.61051640054043
2205	58-59	3.9758706086270834
2205	60-61	4.038692230685118
2205	62-63	3.736826857196615
2205	64-65	3.510489058503353
2205	66-67	3.2005874623504553
2205	68-69	3.213701414628943
2205	70-71	3.0902180668824357
2205	72-73	2.4149750019615084
2205	74-75	2.1430876391116236
2205	76-77	2.3451055316924077
2205	78-79	2.7679051611235046
2205	80-81	2.356605074535203
2205	82-83	2.402121460615202
2205	84-85	1.866848827978039
2205	86-87	1.7272488185568804
2205	88-89	1.8300772535817558
2205	90-91	1.594179007911091
2205	92-93	1.4041363092516868
2205	94-95	1.7011533220333312
2205	96-97	1.4066073413995603
2205	98-99	1.1580062578467576
2205	100-101	0.8517417021961116
2206	1	8.09346173728354
2206	2	8.437365697155702
2206	3	7.325034912484863
2206	4	8.823036932232206
2206	5	8.918291410256657
2206	6	7.637337822840372
2206	7	8.107034089835146
2206	8	8.641135682529171
2206	9	7.4861981729969
2206	10-11	7.888937739432322
2206	12-13	7.5619185145622225
2206	14-15	8.308005774501535
2206	16-17	7.144758217003957
2206	18-19	6.591580899618663
2206	20-21	6.390151763188278
2206	22-23	6.001142985037998
2206	24-25	6.403436814580132
2206	26-27	7.02372400183118
2206	28-29	6.075026022998674
2206	30-31	5.9136216637826315
2206	32-33	5.949821370174327
2206	34-35	5.583255046469684
2206	36-37	5.678663300704677
2206	38-39	5.225514854796597
2206	40-41	4.601820116995842
2206	42-43	4.191954036403114
2206	44-45	4.263473823874449
2206	46-47	4.580993258764906
2206	48-49	4.33349019972097
2206	50-51	4.396484890954966
2206	52-53	4.929402151562126
2206	54-55	4.570469936728202
2206	56-57	4.678928658206619
2206	58-59	4.312043294795217
2206	60-61	4.3801549136054625
2206	62-63	3.8946186684154895
2206	64-65	3.6588422260317017
2206	66-67	3.3183114419590645
2206	68-69	3.5912859232561942
2206	70-71	3.5351584264135703
2206	72-73	2.8881098915876215
2206	74-75	2.4486055174639
2206	76-77	2.6985098408686135
2206	78-79	3.123069957567857
2206	80-81	2.790774420302405
2206	82-83	3.0012670415955682
2206	84-85	2.3879898935947175
2206	86-87	2.3611793543781943
2206	88-89	2.5019708306406834
2206	90-91	2.278007919147992
2206	92-93	2.06123313186675
2206	94-95	2.2753734837054154
2206	96-97	2.076368280372666
2206	98-99	1.8496879532468462
2206	100-101	1.58684675822089
2309	1	8.10177383989296
2309	2	8.186513334513002
2309	3	7.465387232822799
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2104	14-15	-12.823772968386908
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2104	22-23	-2.49790055900635
2104	24-25	-1.6740929836831135
2104	26-27	-0.06420459196330697
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2104	30-31	-3.7361922933286387
2104	32-33	-1.6371605596874943
2104	34-35	-3.8157475874458022
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2104	40-41	-2.0180622333737226
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2104	96-97	-3.4218448280004523
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2110	1	0.4217589997901392
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2110	3	-21.414657610226527
2110	4	-23.39866118097534
2110	5	-12.071784606855719
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2110	9	-14.938843410649163
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2110	36-37	-26.0213716398963
2110	38-39	-15.531173385351096
2110	40-41	-18.389402296903725
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2110	46-47	-4.2854521267426655
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2110	66-67	-3.552027948490206
2110	68-69	-2.146097006171612
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2110	76-77	-2.9408335133340806
2110	78-79	-2.4108496681527853
2110	80-81	-2.342796295366604
2110	82-83	-2.5988010723188957
2110	84-85	-2.651091643601724
2110	86-87	-2.631415745946459
2110	88-89	-2.7359799010163393
2110	90-91	-2.31592002330186
2110	92-93	-2.0546409587485748
2110	94-95	-2.227041727708272
2110	96-97	-2.0615484257610923
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2109	1	7.721342963104387
2109	2	7.822011512816598
2109	3	6.880332279685852
2109	4	8.589206713879747
2109	5	8.025321551804161
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2109	7	7.932088596701668
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2109	9	7.16837553057686
2109	10-11	5.7572905275604
2109	12-13	6.342866910489555
2109	14-15	7.2186973903744125
2109	16-17	2.734648466935621
2109	18-19	4.871360167023141
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2109	26-27	5.150558304881979
2109	28-29	2.227145357671855
2109	30-31	4.410438151915468
2109	32-33	4.784573856326432
2109	34-35	4.638785128592264
2109	36-37	4.6281453225840465
2109	38-39	4.255709390183942
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2109	44-45	3.382400509999833
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2109	60-61	2.892423864858337
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2109	64-65	2.316607478259506
2109	66-67	2.3398392992186636
2109	68-69	2.222240583528521
2109	70-71	2.1111856533921447
2109	72-73	1.7621093234005407
2109	74-75	1.047635960924577
2109	76-77	1.448148031769744
2109	78-79	2.1672077631097153
2109	80-81	1.849054710804733
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2109	84-85	1.2513378586481494
2109	86-87	1.2260360610189167
2109	88-89	1.3366716045463427
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2108	1	6.692687060632043
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2108	3	6.123543368320707
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2108	5	3.0419141364546363
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2108	80-81	1.9241341925059956
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2108	96-97	1.3015075562119414
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2211	1	6.679386021945188
2211	2	6.216238030084025
2211	3	5.794571338698734
2211	4	7.720841875620991
2211	5	7.397877892963919
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2211	7	7.1335376140057605
2211	8	6.7167698878547775
2211	9	5.8295191713541215
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2211	12-13	5.844017168601489
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2211	26-27	5.109901194155793
2211	28-29	4.702334317887701
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2211	32-33	5.195706409878177
2211	34-35	4.720202668365939
2211	36-37	4.929229819381945
2211	38-39	4.43939046956444
2211	40-41	3.8450271811571533
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2211	46-47	4.260592598731371
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2211	54-55	3.5075800072694925
2211	56-57	3.906472483476879
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2211	60-61	0.7288083155218672
2211	62-63	1.355865969321247
2211	64-65	2.935077683927208
2211	66-67	3.0477431862426485
2211	68-69	3.1615770450913345
2211	70-71	3.116322992992334
2211	72-73	2.6762348518823913
2211	74-75	2.1968149236307823
2211	76-77	2.280289829874487
2211	78-79	2.6848806455194207
2211	80-81	2.6577095563254574
2211	82-83	2.82623652455322
2211	84-85	2.2245015227996277
2211	86-87	2.303224793420519
2211	88-89	2.380259818832265
2211	90-91	2.187554981884393
2211	92-93	1.873175096844566
2211	94-95	2.2155079729793847
2211	96-97	2.049326057023194
2211	98-99	1.8650090209202652
2211	100-101	1.772980769171749
2210	1	7.3364256286502645
2210	2	7.604852558360594
2210	3	6.906931258714227
2210	4	8.442227508432204
2210	5	8.49366838609432
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2210	7	7.949433003602749
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2210	14-15	5.105318218122957
2210	16-17	1.7097229564800571
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2210	32-33	5.443490346558995
2210	34-35	5.217844657506145
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2210	38-39	4.310537506593743
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2210	46-47	4.502989543267965
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2210	58-59	4.191614585539909
2210	60-61	4.108562861118028
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2210	64-65	3.5072570236585
2210	66-67	3.178399270209386
2210	68-69	3.4601481079909817
2210	70-71	3.4488004884986942
2210	72-73	2.9598565264376404
2210	74-75	2.684012406094343
2210	76-77	2.8498532191542587
2210	78-79	3.3996392422076696
2210	80-81	3.113258446112031
2210	82-83	3.0910987307760287
2210	84-85	2.583109469489841
2210	86-87	2.629040933141333
2210	88-89	2.7640864997124197
2210	90-91	2.7204241463911245
2210	92-93	2.343787641086177
2210	94-95	2.6314943461925075
2210	96-97	2.5600316675157764
2210	98-99	2.4585967714515853
2210	100-101	2.252233105918137
2209	1	5.451603672391375
2209	2	3.001930006153387
2209	3	4.774561555700714
2209	4	2.2007177010122376
2209	5	4.990554432683744
2209	6	5.371701981410553
2209	7	6.291642305557858
2209	8	7.2691395046030784
2209	9	6.502510039097821
2209	10-11	6.235955805614225
2209	12-13	5.7386990435635425
2209	14-15	5.146474554594004
2209	16-17	4.704906663402834
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2209	22-23	4.039173751988844
2209	24-25	4.426690761131866
2209	26-27	1.7078312834276232
2209	28-29	0.6275274537961302
2209	30-31	3.204994652682867
2209	32-33	4.28894462771725
2209	34-35	4.289250428853794
2209	36-37	4.019001030911156
2209	38-39	-0.7915664331009395
2209	40-41	-0.1282214985930601
2209	42-43	-4.10780671193961
2209	44-45	0.6445688970326877
2209	46-47	2.523338864403023
2209	48-49	1.7409760452122072
2209	50-51	2.9543714992488184
2209	52-53	3.955882368113244
2209	54-55	3.3121609254014963
2209	56-57	3.759915592220036
2209	58-59	2.9770974852800904
2209	60-61	3.044024394494162
2209	62-63	2.9497603313152716
2209	64-65	2.320088371559457
2209	66-67	2.535048888920347
2209	68-69	-0.4313926039928191
2209	70-71	1.8566810925395423
2209	72-73	2.3333696425575567
2209	74-75	1.9124995838863867
2209	76-77	2.2257613394080558
2209	78-79	2.5909538251792554
2209	80-81	2.2817803158700016
2209	82-83	2.349784267280846
2209	84-85	1.7103811142339183
2209	86-87	1.744337457652314
2209	88-89	1.9726504888862593
2209	90-91	2.0081603711597005
2209	92-93	1.83150387346938
2209	94-95	1.9221174196354518
2209	96-97	1.614540189369606
2209	98-99	1.4213016137506962
2209	100-101	1.4061471928236813
2208	1	4.525475885186019
2208	2	6.248470546541068
2208	3	4.700566709459672
2208	4	3.050958823927825
2208	5	3.8818207124776904
2208	6	4.689238749147481
2208	7	3.523427459155844
2208	8	3.9865559882350183
2208	9	4.022899766558993
2208	10-11	4.425410150405746
2208	12-13	5.2406360266132594
2208	14-15	5.6883685871145495
2208	16-17	3.9314772851495228
2208	18-19	1.9519979956546862
2208	20-21	4.263416468521399
2208	22-23	3.558466385955139
2208	24-25	3.9518388838449283
2208	26-27	5.050583635868858
2208	28-29	4.35332581112792
2208	30-31	4.407777186547644
2208	32-33	3.709184813071584
2208	34-35	3.905021704587508
2208	36-37	3.730160598397397
2208	38-39	3.9285046703694952
2208	40-41	3.525480193346066
2208	42-43	3.1792258153448394
2208	44-45	-1.33271324342617
2208	46-47	2.7155840853966495
2208	48-49	2.896961655931122
2208	50-51	3.219161555139216
2208	52-53	1.8825190433339785
2208	54-55	1.0211032747385573
2208	56-57	-0.9513760985291917
2208	58-59	2.0376092871314135
2208	60-61	3.1651173363982075
2208	62-63	2.511038904908279
2208	64-65	2.8188488492414763
2208	66-67	3.0039068729722374
2208	68-69	2.0739873062666874
2208	70-71	-0.09980499859793568
2208	72-73	0.19225927854668612
2208	74-75	-1.85344075197807
2208	76-77	0.6952981580473825
2208	78-79	2.0820713296653004
2208	80-81	2.2531701231652725
2208	82-83	2.341559777702461
2208	84-85	1.4802057761408491
2208	86-87	2.0020217346631597
2208	88-89	2.436372143701444
2208	90-91	2.232355248594775
2208	92-93	1.9975558139274945
2208	94-95	2.0385036844284166
2208	96-97	1.9930110693915672
2208	98-99	2.2440325741331897
2208	100-101	2.1056900555788545
1307	1	7.27853688565072
1307	2	7.171792097648002
1307	3	6.822904578828201
1307	4	8.48989603295501
1307	5	5.005572509999034
1307	6	5.974494805224047
1307	7	3.745394176195795
1307	8	5.69333485438478
1307	9	5.884597163369989
1307	10-11	6.661125542555077
1307	12-13	5.788095804214585
1307	14-15	5.778164154509252
1307	16-17	4.446282305158572
1307	18-19	5.169849410314654
1307	20-21	5.236264230203311
1307	22-23	4.695719108612995
1307	24-25	5.417626609838635
1307	26-27	6.295144936987679
1307	28-29	5.554968553532586
1307	30-31	5.183308606427897
1307	32-33	5.607964876959066
1307	34-35	5.453602470773742
1307	36-37	5.0143248775166285
1307	38-39	4.481959570421818
1307	40-41	3.6709819076234176
1307	42-43	4.230196263423149
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1212	60-61	-14.363908406209871
1212	62-63	-11.95394819414766
1212	64-65	-16.302132416256736
1212	66-67	-12.474346521061179
1212	68-69	-8.126704763068851
1212	70-71	-15.313645430799657
1212	72-73	-11.901075133214476
1212	74-75	-8.707938398861952
1212	76-77	-19.088561921970623
1212	78-79	-9.714140851679371
1212	80-81	-7.853641957101907
1212	82-83	-5.397144235053798
1212	84-85	-4.61099518218629
1212	86-87	-3.5945055100408183
1212	88-89	-3.205579561152284
1212	90-91	-3.8172070085077756
1212	92-93	-3.997838679660209
1212	94-95	-3.5844237748894017
1212	96-97	-13.09284840576909
1212	98-99	-10.325717801641975
1212	100-101	-6.662402162057258
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	424.0
3	1039.0
4	472.0
5	2857.0
6	3647.0
7	3473.0
8	6978.0
9	13445.0
10	17206.0
11	21041.0
12	27093.0
13	34550.0
14	39698.0
15	42282.0
16	44340.0
17	49118.0
18	55349.0
19	61222.0
20	65863.0
21	73562.0
22	81947.0
23	87809.0
24	93029.0
25	95954.0
26	101039.0
27	105491.0
28	106437.0
29	108437.0
30	111920.0
31	119316.0
32	129508.0
33	148088.0
34	168486.0
35	185059.0
36	195042.0
37	182122.0
38	68481.0
39	215.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.612314879127766	29.64896327929341	11.840768113306353	22.89795372827247
2	23.83637128971772	26.995165631337937	29.597858761602275	19.570604317342074
3	20.86602439395521	26.269926403981238	29.537753956985917	23.326295245077635
4	24.01591867629044	25.52251073860279	27.280008934038303	23.18156165106847
5	22.710366468629843	27.637885823488677	26.71479994260606	22.936947765275416
6	22.650508893203696	27.79398160070879	26.34925274090826	23.206256765179255
7	24.046311022770887	27.87656930061691	26.909891852122485	21.16722782448972
8	21.610518184939114	27.106051885808142	28.386284389157023	22.89714554009572
9	22.383347724612843	26.842499999001067	27.780656921619407	22.993495354766683
10-11	22.993976693739597	27.079190838924482	27.030802603036875	22.89602986429905
12-13	22.8735054869679	27.128171385999387	26.923353330708892	23.074969796323817
14-15	22.818827673924734	27.502372080994224	26.89024056939734	22.7885596756837
16-17	23.0141289902644	27.453953937257413	26.785014674382325	22.746902398095862
18-19	23.150304846150743	27.399875579361606	26.56696259918727	22.88285697530038
20-21	22.92535140446889	27.595976292734946	26.726642396463774	22.75202990633239
22-23	22.930927557371316	27.30137204694561	26.902940902466128	22.864759493216948
24-25	22.979062988676834	27.219797240627237	26.9090983079126	22.89204146278333
26-27	22.718616893440895	27.42523618900808	27.00348174026585	22.852665177285175
28-29	23.071854732019904	27.311890010772157	26.845160719328693	22.771094537879254
30-31	23.063260889378405	27.21564499012128	26.71064461165581	23.01044950884451
32-33	22.794361616120707	27.371128440066855	26.96251462325358	22.871995320558856
34-35	23.097911807924078	27.224358838562317	26.866151952901575	22.81157740061203
36-37	23.015619335700727	27.26725942441949	26.778234278341472	22.938886961538312
38-39	22.876726086345204	27.33893128748801	27.05766900270779	22.726673623458993
40-41	23.096039854495825	27.3113443564463	26.779996878018697	22.81261891103918
42-43	23.032084344660195	27.288095418689316	26.805863167475728	22.873957069174757
44-45	22.81546633339138	27.2835050334419	26.995642201352588	22.90538643181413
46-47	23.05403282067802	27.323796933173593	26.784756119372894	22.8374141267755
48-49	23.010641036101788	27.19386933234936	26.85356331490049	22.941926316648356
50-51	22.92391251237115	27.36146945798791	26.843552965770833	22.87106506387011
52-53	22.973580008651382	27.258316509051063	26.965964780051365	22.802138702246186
54-55	22.971688339944425	27.18228425289561	26.857963502479592	22.988063904680374
56-57	22.765687681850338	27.318675307907135	27.086986996817863	22.82865001342466
58-59	23.03514825713014	27.256468519777805	26.81414299693409	22.894240226157965
60-61	23.01488274677862	27.114339853045767	26.773388123384002	23.097389276791613
62-63	22.90243577576246	27.27942490662208	26.954447361720423	22.86369195589504
64-65	23.099814051417763	27.227715964686578	26.77884953291305	22.89362045098261
66-67	23.14357308704362	27.11866978068615	26.785238849795086	22.952518282475147
68-69	22.94909244727924	27.274357061040583	26.945632387678764	22.83091810400141
70-71	23.219509314303078	27.25156381419211	26.6922198398418	22.83670703166301
72-73	23.07561383739036	27.191783096573864	26.77914911506729	22.953453950968488
74-75	23.03494228628524	27.253824460770566	26.811728233121208	22.89950501982299
76-77	23.228975210265418	27.310703348505566	26.626787855115612	22.8335335861134
78-79	23.22170143064609	27.09356437602945	26.720432603751526	22.964301589572937
80-81	23.176983950779313	27.19155847225852	26.738426031863945	22.89303154509822
82-83	23.169138543558955	27.20908657586535	26.75688046009035	22.864894420485353
84-85	23.32164036597969	27.05739062321365	26.55273286795399	23.06823614285267
86-87	23.109604255347257	27.239563330562238	26.774113492912	22.876718921178504
88-89	23.319189227232915	27.154589268065155	26.64873369116261	22.87748781353932
90-91	23.414001666631442	27.06728722520961	26.441111600902158	23.077599507256792
92-93	23.28890947111404	27.156569372843148	26.646517155099993	22.90800400094282
94-95	23.536782882959677	27.0792297710719	26.42803966697923	22.955947678989194
96-97	23.508399635620535	27.075767724556982	26.407896427805568	23.007936212016915
98-99	23.468586930684104	27.112510517558192	26.549014651005727	22.86988790075198
100-101	23.882250426474833	27.031313750357405	26.09304077358292	22.993395049584844
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	169.0
1	178.0
2	211.5
3	250.5
4	284.0
5	322.0
6	386.5
7	485.0
8	634.5
9	813.5
10	1045.0
11	1404.5
12	1861.0
13	2455.0
14	3212.5
15	4034.5
16	4979.0
17	6093.0
18	7515.5
19	9277.5
20	11480.5
21	14193.0
22	17280.5
23	20798.5
24	24666.0
25	29009.0
26	33784.5
27	38765.0
28	43905.0
29	48915.5
30	53842.0
31	58434.0
32	62780.0
33	67151.5
34	71216.0
35	74520.5
36	77021.0
37	78777.0
38	79420.5
39	79264.0
40	78080.5
41	75574.0
42	72395.5
43	69019.5
44	65697.0
45	62817.0
46	60244.5
47	57744.5
48	55418.0
49	53245.0
50	51323.5
51	49304.5
52	47494.0
53	46028.5
54	45156.0
55	44646.0
56	44137.0
57	43845.5
58	43567.5
59	43289.0
60	42709.5
61	42158.0
62	41472.0
63	40400.5
64	39107.0
65	37293.0
66	35345.5
67	33135.0
68	30270.5
69	27512.0
70	24656.5
71	21544.5
72	18429.0
73	15593.0
74	12935.5
75	10438.5
76	8320.5
77	6515.5
78	5140.0
79	4043.0
80	3209.5
81	2568.5
82	2026.0
83	1606.5
84	1281.5
85	1015.0
86	812.0
87	637.0
88	504.0
89	408.5
90	328.5
91	261.0
92	187.0
93	128.0
94	91.0
95	66.0
96	48.0
97	33.0
98	18.5
99	6.0
100	4.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	13.954696744655715
2	11.316915022742878
3	10.046911074837135
4	11.874259767673099
5	9.336363454685245
6	5.561230434394064
7	10.003887574805649
8	10.37416870566383
9	5.632119286330254
10-11	6.567173408837503
12-13	5.557120389255211
14-15	7.564179863116643
16-17	2.793209300466547
18-19	3.233153811086489
20-21	3.1883392363385306
22-23	3.265487423073341
24-25	3.6330348083116424
26-27	4.529175475926259
28-29	1.796297113277746
30-31	2.361767681395334
32-33	3.1753303778715174
34-35	2.103061078664379
36-37	3.26650550764902
38-39	1.4436627817313397
40-41	1.203149727436135
42-43	0.5746144758806337
44-45	1.3719066725640157
46-47	1.441513492071572
48-49	1.3357081098731955
50-51	1.6078760531047998
52-53	2.5444950093117034
54-55	1.908041322167585
56-57	1.903573062085437
58-59	1.6038791284743552
60-61	1.4672672611526452
62-63	0.6327207103666274
64-65	1.28621788744434
66-67	0.5792147098892588
68-69	0.8305496261555732
70-71	1.034920678014162
72-73	0.08419936509229314
74-75	0.10716282829928217
76-77	0.4697706180037322
78-79	0.9206124042670565
80-81	0.30580244106515775
82-83	0.5226733091029204
84-85	0.06515741284347629
86-87	0.10054527855736663
88-89	0.0818992480879806
90-91	0.08985539051273378
92-93	0.17582697690343166
94-95	0.4737486892161088
96-97	0.38790907675188785
98-99	0.06444098295688713
100-101	0.23455537418567374
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2652039.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.8953766258566
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.06533932602206	97.97104043215234
2	0.8037956382058118	1.5898334474116906
3	0.08451538580198469	0.2507454272870055
4	0.04126341671064013	0.16323044545873683
5	0.005086233259493034	0.025150247690226092
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	1.1312050840881299E-4	0.0	0.0
2	1.50827344545084E-4	0.0	0.0
3	1.50827344545084E-4	0.0	0.0
4	1.88534180681355E-4	0.0	0.0
5	2.2624101681762598E-4	0.0	0.0
6	3.01654689090168E-4	0.0	0.0
7	3.01654689090168E-4	0.0	0.0
8	3.01654689090168E-4	0.0	0.0
9	3.01654689090168E-4	0.0	0.0
10-11	3.39361525226439E-4	1.88534180681355E-5	3.7706836136271E-5
12-13	4.3362861556711646E-4	3.7706836136271E-5	3.7706836136271E-5
14-15	5.844559601122005E-4	3.7706836136271E-5	3.7706836136271E-5
16-17	6.78723050452878E-4	3.7706836136271E-5	3.7706836136271E-5
18-19	7.5413672272542E-4	3.7706836136271E-5	3.7706836136271E-5
20-21	7.5413672272542E-4	3.7706836136271E-5	3.7706836136271E-5
22-23	7.5413672272542E-4	3.7706836136271E-5	7.5413672272542E-5
24-25	7.5413672272542E-4	5.65602542044065E-5	7.5413672272542E-5
26-27	8.106969769298264E-4	7.5413672272542E-5	7.5413672272542E-5
28-29	9.426709034067749E-4	7.5413672272542E-5	9.42670903406775E-5
30-31	0.001055791411815588	7.5413672272542E-5	1.1312050840881299E-4
32-33	0.0012631790105650785	7.5413672272542E-5	1.1312050840881299E-4
34-35	0.0017345144622684658	7.5413672272542E-5	1.1312050840881299E-4
36-37	0.002337823840448802	7.5413672272542E-5	1.1312050840881299E-4
38-39	0.0030354003089698156	1.1312050840881299E-4	1.88534180681355E-4
40-41	0.0038083904497633707	1.1312050840881299E-4	2.2624101681762598E-4
42-43	0.004958448951919636	1.1312050840881299E-4	2.6394785295389696E-4
44-45	0.006598696323847424	1.1312050840881299E-4	2.6394785295389696E-4
46-47	0.008842253073955548	1.1312050840881299E-4	2.6394785295389696E-4
48-49	0.012424402506901293	1.1312050840881299E-4	2.6394785295389696E-4
50-51	0.01738285145882093	1.1312050840881299E-4	3.01654689090168E-4
52-53	0.022605248263694463	1.1312050840881299E-4	3.01654689090168E-4
54-55	0.029637573203109002	1.1312050840881299E-4	3.205081071583035E-4
56-57	0.03693384599547744	1.1312050840881299E-4	3.39361525226439E-4
58-59	0.04675647680897604	1.1312050840881299E-4	3.39361525226439E-4
60-61	0.05887922462678716	1.1312050840881299E-4	3.39361525226439E-4
62-63	0.07294387450561625	1.1312050840881299E-4	3.39361525226439E-4
64-65	0.08962914949591616	1.88534180681355E-4	3.7706836136271E-4
66-67	0.1079169650220076	1.88534180681355E-4	3.7706836136271E-4
68-69	0.13291659738035527	1.88534180681355E-4	3.7706836136271E-4
70-71	0.16006551939847036	2.2624101681762598E-4	3.7706836136271E-4
72-73	0.19117365921089396	2.2624101681762598E-4	4.1477519749898097E-4
74-75	0.22716483430296464	2.2624101681762598E-4	4.1477519749898097E-4
76-77	0.2666627451557085	2.2624101681762598E-4	4.5248203363525195E-4
78-79	0.3095542712607168	2.2624101681762598E-4	4.7133545170338745E-4
80-81	0.3622118679250192	2.2624101681762598E-4	4.901888697715229E-4
82-83	0.41943199176181045	2.2624101681762598E-4	4.901888697715229E-4
84-85	0.48411806915358335	2.2624101681762598E-4	5.278957059077939E-4
86-87	0.5592300867370352	2.2624101681762598E-4	5.656025420440649E-4
88-89	0.6422228330729676	2.2624101681762598E-4	5.656025420440649E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCACGC	1030	0.0011906067	6.5855656	1
GGTATAA	955	0.003914671	6.5108604	1
GTAACAA	1225	0.008261289	5.5372515	1
GATCGGA	3470	0.0	5.157379	94-95
GTTCAGG	1450	0.009275954	5.067858	1
>>END_MODULE