##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename NYCLib27_ATTCCT_L007_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2652039 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.24459067155498 33.0 33.0 33.0 27.0 33.0 2 31.667147428827406 33.0 33.0 33.0 27.0 33.0 3 31.83044894890309 33.0 33.0 33.0 27.0 33.0 4 35.651740792650486 37.0 37.0 37.0 33.0 37.0 5 35.15851878498016 37.0 37.0 37.0 33.0 37.0 6 34.962576719271475 37.0 37.0 37.0 33.0 37.0 7 34.75970526828603 37.0 33.0 37.0 27.0 37.0 8 34.8038882535287 37.0 33.0 37.0 27.0 37.0 9 34.70943149780226 37.0 33.0 37.0 27.0 37.0 10-11 34.566982612246655 37.0 33.0 37.0 27.0 37.0 12-13 34.57634710500109 37.0 33.0 37.0 27.0 37.0 14-15 35.51437799368712 37.0 33.0 40.0 27.0 40.0 16-17 35.42096986507363 37.0 33.0 40.0 27.0 40.0 18-19 35.64076697212975 37.0 33.0 40.0 27.0 40.0 20-21 35.31680812386243 37.0 33.0 40.0 27.0 40.0 22-23 35.3462509412569 37.0 33.0 40.0 27.0 40.0 24-25 35.183370606540855 37.0 33.0 40.0 27.0 40.0 26-27 34.8037076377836 37.0 33.0 40.0 27.0 40.0 28-29 34.440834203418575 37.0 33.0 40.0 27.0 40.0 30-31 34.333440232213775 37.0 33.0 40.0 27.0 40.0 32-33 34.328142798805 37.0 33.0 40.0 24.5 40.0 34-35 34.18275787045364 37.0 33.0 40.0 24.5 40.0 36-37 33.86930980275931 37.0 33.0 37.0 22.0 40.0 38-39 33.76950527499784 37.0 33.0 37.0 22.0 40.0 40-41 33.47944430681449 37.0 33.0 37.0 22.0 40.0 42-43 33.0896966447326 37.0 33.0 37.0 22.0 40.0 44-45 32.95604042776143 37.0 33.0 37.0 22.0 40.0 46-47 32.81167678906683 37.0 33.0 37.0 22.0 40.0 48-49 32.8234505601162 37.0 33.0 37.0 22.0 40.0 50-51 33.1103002256 37.0 33.0 37.0 22.0 40.0 52-53 32.95603967362471 37.0 33.0 37.0 22.0 40.0 54-55 32.5898527510342 37.0 33.0 37.0 22.0 40.0 56-57 32.63313944478192 37.0 33.0 37.0 22.0 40.0 58-59 32.34524492286879 37.0 33.0 37.0 22.0 40.0 60-61 32.25916983121289 35.0 27.0 37.0 22.0 40.0 62-63 32.23673275543837 33.0 27.0 37.0 22.0 40.0 64-65 31.904184666967566 33.0 27.0 37.0 22.0 40.0 66-67 31.56177774912058 33.0 27.0 37.0 18.5 40.0 68-69 31.380059833207582 33.0 27.0 37.0 15.0 40.0 70-71 31.228596185802697 33.0 27.0 37.0 18.5 38.5 72-73 31.018445618635322 33.0 27.0 37.0 15.0 37.0 74-75 30.671193183810644 33.0 27.0 37.0 15.0 37.0 76-77 29.58886294658563 33.0 27.0 35.0 15.0 37.0 78-79 30.523199696535382 33.0 27.0 37.0 15.0 37.0 80-81 30.243574283786927 33.0 27.0 37.0 15.0 37.0 82-83 30.229759441697503 33.0 27.0 37.0 15.0 37.0 84-85 30.101986245300314 33.0 27.0 37.0 15.0 37.0 86-87 29.9068769350677 33.0 27.0 37.0 15.0 37.0 88-89 29.78327128673447 33.0 27.0 37.0 15.0 37.0 90-91 29.703496441794407 33.0 27.0 37.0 15.0 37.0 92-93 29.49430589067506 33.0 27.0 35.0 15.0 37.0 94-95 29.400586115060904 33.0 27.0 33.0 15.0 37.0 96-97 29.272732603102746 33.0 27.0 33.0 15.0 37.0 98-99 29.003642480370765 33.0 27.0 33.0 15.0 37.0 100-101 28.19761134734444 33.0 24.5 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 1.1294915404704042 2306 2 0.5933518836340639 2306 3 0.44050021238231096 2306 4 0.29393649143948863 2306 5 0.6459523254753066 2306 6 0.7140717180160081 2306 7 0.9265100837206504 2306 8 0.7936280427345324 2306 9 1.0039927994810043 2306 10-11 1.221782081049561 2306 12-13 1.341502974598221 2306 14-15 1.4707780380347941 2306 16-17 1.4543859471198104 2306 18-19 1.3794464792779024 2306 20-21 1.5954651145815717 2306 22-23 1.3278558037940869 2306 24-25 1.2379645019080456 2306 26-27 1.3896293691646946 2306 28-29 1.6195801657502216 2306 30-31 1.5769934815502253 2306 32-33 1.2606579172804402 2306 34-35 1.281395225947513 2306 36-37 1.4162915098542612 2306 38-39 1.1987207900182213 2306 40-41 1.3686095996565797 2306 42-43 1.3919819130522555 2306 44-45 1.228219077361409 2306 46-47 1.1162993596070905 2306 48-49 1.0773865829687779 2306 50-51 1.1445270530691118 2306 52-53 1.4526576968591982 2306 54-55 1.7575378899209326 2306 56-57 1.3664247839563401 2306 58-59 1.2845406518308593 2306 60-61 0.9926030220161337 2306 62-63 0.8434116795388356 2306 64-65 0.9449961703881193 2306 66-67 1.021842284651246 2306 68-69 0.9287202234433245 2306 70-71 0.793574932301329 2306 72-73 0.6358393065341446 2306 74-75 0.754554518902637 2306 76-77 0.8063841203014626 2306 78-79 0.9435761661835471 2306 80-81 1.3856645557132943 2306 82-83 1.2636904760883425 2306 84-85 0.9837456398355471 2306 86-87 0.8261603102835444 2306 88-89 0.5934652129753175 2306 90-91 0.29223134373457427 2306 92-93 0.2205175081333408 2306 94-95 0.30338165177974474 2306 96-97 0.39127840146681336 2306 98-99 0.46879029838294883 2306 100-101 0.39286166780583187 2307 1 0.7352177030643503 2307 2 0.4721678740357298 2307 3 0.41157264110457703 2307 4 0.34246571849418217 2307 5 0.604109496949647 2307 6 0.6753649339284706 2307 7 0.9247183237602528 2307 8 0.8792583136550789 2307 9 1.0202043871946174 2307 10-11 1.108254795016542 2307 12-13 1.3586586891295482 2307 14-15 1.401862633135437 2307 16-17 1.4112514937744365 2307 18-19 1.2757964945385183 2307 20-21 1.4862213147027603 2307 22-23 1.194125030685278 2307 24-25 1.2320809270391067 2307 26-27 1.4191703689432273 2307 28-29 1.6483915172711079 2307 30-31 1.6150304149688495 2307 32-33 1.1963601651743048 2307 34-35 1.282469755482012 2307 36-37 1.441699307112998 2307 38-39 0.9681274610632329 2307 40-41 1.2143871745512769 2307 42-43 1.2560623654899175 2307 44-45 1.038433437626928 2307 46-47 1.0274092065698568 2307 48-49 1.1021327513399655 2307 50-51 1.1628022924205013 2307 52-53 1.2445726788710019 2307 54-55 1.6633907205919911 2307 56-57 1.3155052636451074 2307 58-59 1.2116587096745448 2307 60-61 1.0406957824140406 2307 62-63 0.942150442794631 2307 64-65 1.0185777071719038 2307 66-67 1.1954854089256948 2307 68-69 0.975423967298827 2307 70-71 1.0551230667899816 2307 72-73 0.8183677145693551 2307 74-75 1.1450414440716052 2307 76-77 1.1018046624051614 2307 78-79 1.1283226436069995 2307 80-81 1.7119231726356325 2307 82-83 1.5738766097862111 2307 84-85 1.1660796824820352 2307 86-87 1.1920927847777527 2307 88-89 0.9460471093497809 2307 90-91 0.7910513555318239 2307 92-93 0.8112380216977009 2307 94-95 0.8281924176496744 2307 96-97 0.769036739436963 2307 98-99 0.7445940572513265 2307 100-101 0.8498184746851969 1101 1 1.0050169378980236 1101 2 0.5550547845149083 1101 3 0.32486192109195855 1101 4 0.30600240360923436 1101 5 0.44152884004707715 1101 6 0.48660326382758967 1101 7 0.63874398028873 1101 8 0.6058235782582528 1101 9 0.7600272352148707 1101 10-11 0.9010307573891296 1101 12-13 1.1319391122762354 1101 14-15 1.2026025483686027 1101 16-17 1.263841020155887 1101 18-19 0.8747836324039397 1101 20-21 1.203872803582172 1101 22-23 0.8851003422418344 1101 24-25 1.0809969751086967 1101 26-27 0.9966717692542204 1101 28-29 1.209655629069509 1101 30-31 1.2241777064539505 1101 32-33 0.777703763579261 1101 34-35 0.9333068836413005 1101 36-37 1.0440149640054628 1101 38-39 0.874327287465114 1101 40-41 1.0628021817398832 1101 42-43 1.1860274790414849 1101 44-45 0.9453760102156039 1101 46-47 0.8776837332445169 1101 48-49 0.8692446023185596 1101 50-51 0.7551450936086894 1101 52-53 1.0395251896235678 1101 54-55 1.4902602116067527 1101 56-57 1.1454017265707392 1101 58-59 1.1454361952012917 1101 60-61 0.7894792938278457 1101 62-63 0.5246713514094274 1101 64-65 0.5505673570031568 1101 66-67 0.7910966977577836 1101 68-69 0.5757590308840683 1101 70-71 0.7243963382400125 1101 72-73 0.6197279119677077 1101 74-75 0.8443064577309585 1101 76-77 0.7656598171673288 1101 78-79 0.7756797840809426 1101 80-81 1.3606885229762575 1101 82-83 1.16263041893545 1101 84-85 0.8692029447314731 1101 86-87 0.8506584163938449 1101 88-89 0.7329731714123717 1101 90-91 0.6058045545094473 1101 92-93 0.7421305885233735 1101 94-95 0.7118743458526424 1101 96-97 0.6004566092939783 1101 98-99 0.7208175853070848 1101 100-101 0.7532972996574436 2201 1 1.4274479425862303 2201 2 0.806275910890605 2201 3 0.35760588333197063 2201 4 0.2509162252282735 2201 5 0.39225182709666484 2201 6 0.4997068915928935 2201 7 0.7341378000103873 2201 8 0.6841677387144713 2201 9 0.6457027081545519 2201 10-11 0.9067900912252327 2201 12-13 1.1735082183529428 2201 14-15 1.1587363634830439 2201 16-17 1.1002928441057875 2201 18-19 0.9978915772253529 2201 20-21 1.25113416007639 2201 22-23 1.0406091051592554 2201 24-25 0.8902724451720232 2201 26-27 1.167322682156417 2201 28-29 1.364106164462683 2201 30-31 1.3786301657251343 2201 32-33 1.043868028951806 2201 34-35 1.023202701546822 2201 36-37 1.126247061153606 2201 38-39 0.6874257869916462 2201 40-41 1.0541541923753286 2201 42-43 0.9888658425063284 2201 44-45 0.9936607751959272 2201 46-47 1.0129518802081066 2201 48-49 1.051214888054183 2201 50-51 0.8848556616156955 2201 52-53 1.0412657946018342 2201 54-55 1.3815282717750605 2201 56-57 1.0889094456739485 2201 58-59 1.0929845942950962 2201 60-61 1.0273588938832532 2201 62-63 0.8896798644119421 2201 64-65 0.8858487061853815 2201 66-67 0.9167923531239843 2201 68-69 0.7698848509922627 2201 70-71 1.054559982673041 2201 72-73 0.8564337887401052 2201 74-75 0.8495172024824278 2201 76-77 0.9627830707591798 2201 78-79 0.8395293845072516 2201 80-81 1.5566526873735889 2201 82-83 1.1731465884678371 2201 84-85 1.088698786505688 2201 86-87 1.1224422663651659 2201 88-89 1.0886956298685924 2201 90-91 0.8357468015838272 2201 92-93 1.0759753054534258 2201 94-95 0.8812043430343195 2201 96-97 0.6654348322564267 2201 98-99 0.7973958465200148 2201 100-101 0.8916451337744995 2304 1 -1.9346468230376068 2304 2 -0.3411059810401227 2304 3 -0.19118498430761832 2304 4 0.02905806753047102 2304 5 0.3616033378895409 2304 6 0.5072871073076044 2304 7 0.44236730436906413 2304 8 0.6714558205387391 2304 9 0.9378959218874812 2304 10-11 0.860740665408052 2304 12-13 1.0485066321687313 2304 14-15 1.1564550736995614 2304 16-17 1.1422384148291442 2304 18-19 0.7279197335812455 2304 20-21 1.1450819235407934 2304 22-23 0.8752577288748 2304 24-25 0.9223088059071145 2304 26-27 0.9747935463317816 2304 28-29 1.394985919327432 2304 30-31 1.4050069418945625 2304 32-33 0.8874233149265436 2304 34-35 0.8474371931858897 2304 36-37 1.0780456592205567 2304 38-39 0.9902613847086812 2304 40-41 0.9081847181731888 2304 42-43 0.8993487039445682 2304 44-45 0.8448243523458743 2304 46-47 0.9351893579975652 2304 48-49 0.7679036317613992 2304 50-51 0.7405705960793227 2304 52-53 1.1366403942092802 2304 54-55 1.4293327707645034 2304 56-57 1.0402802895144276 2304 58-59 0.9143559044737302 2304 60-61 0.6084564873562854 2304 62-63 0.31994474289912844 2304 64-65 0.35746346391635697 2304 66-67 0.5752466039231869 2304 68-69 0.5146550826925385 2304 70-71 0.47567063520067165 2304 72-73 0.39087920639668283 2304 74-75 0.6718374870184967 2304 76-77 0.6488095254566488 2304 78-79 0.650576697527061 2304 80-81 1.3369988102159418 2304 82-83 1.0700767747905502 2304 84-85 0.7016998184771346 2304 86-87 0.49505418672983836 2304 88-89 0.39027988686342496 2304 90-91 0.34650109086385683 2304 92-93 0.3369072077102153 2304 94-95 0.065228517750608 2304 96-97 0.15161877125305168 2304 98-99 0.5851980265081451 2304 100-101 0.6579221992321962 1102 1 1.4435837835662504 1102 2 0.1416159257605969 1102 3 0.2292056881722786 1102 4 0.1601869824241433 1102 5 0.29980543061056864 1102 6 0.16307572353812816 1102 7 -4.84312382304865 1102 8 -1.215784757941492 1102 9 0.25247372874166985 1102 10-11 0.6096391982752962 1102 12-13 0.7981402747188113 1102 14-15 0.7329930033369294 1102 16-17 0.5372323693325214 1102 18-19 0.8350980246428108 1102 20-21 1.1085949655289653 1102 22-23 0.6562407216581647 1102 24-25 0.8789343633150253 1102 26-27 0.934015004155043 1102 28-29 0.9030715936646203 1102 30-31 1.1849061179827771 1102 32-33 0.9162095821410432 1102 34-35 1.0461407197610626 1102 36-37 1.1730747726860855 1102 38-39 0.9050664032513964 1102 40-41 1.040332834969064 1102 42-43 1.021833206150788 1102 44-45 1.1182957978404247 1102 46-47 1.010072803022389 1102 48-49 0.9202194135232276 1102 50-51 0.6715601789506991 1102 52-53 0.9726784279753602 1102 54-55 1.4099486876630465 1102 56-57 1.1450697845416187 1102 58-59 0.9580654836411711 1102 60-61 0.8391167077401889 1102 62-63 0.6672580358605593 1102 64-65 0.5614779539499857 1102 66-67 0.705785515244191 1102 68-69 0.7594772514292991 1102 70-71 0.6822969465217525 1102 72-73 0.5882793058067861 1102 74-75 0.5598882560631395 1102 76-77 0.5643138206329859 1102 78-79 0.6229552704989381 1102 80-81 1.2358408262045764 1102 82-83 1.092247986164029 1102 84-85 0.7760877208139796 1102 86-87 0.7124596380364494 1102 88-89 0.5143012188807532 1102 90-91 0.009377599532793823 1102 92-93 0.19809742550065224 1102 94-95 0.33824140665006297 1102 96-97 0.15991154997120205 1102 98-99 0.1795159337729224 1102 100-101 0.21696384433103333 2202 1 1.557989821102911 2202 2 0.965511252867735 2202 3 0.4213844822743553 2202 4 -0.153070270847536 2202 5 0.17913869368127422 2202 6 0.5845682041008686 2202 7 0.672248283725942 2202 8 -3.9208987983364523 2202 9 -0.5518986030214492 2202 10-11 0.43818192952780777 2202 12-13 0.11705093224850316 2202 14-15 0.1266722313316464 2202 16-17 0.25092005202701984 2202 18-19 0.814082439574598 2202 20-21 1.1371526895763395 2202 22-23 -2.8467692364750334 2202 24-25 -2.922950343058524 2202 26-27 -1.3023091663458857 2202 28-29 0.6703127764376973 2202 30-31 1.2435331141521786 2202 32-33 1.1786447626955336 2202 34-35 -2.195576751259715 2202 36-37 -0.32479976679505285 2202 38-39 0.35648849206264543 2202 40-41 1.3767711274945214 2202 42-43 1.4663225763848118 2202 44-45 1.4895014590888564 2202 46-47 0.9450424488920035 2202 48-49 1.195896748291176 2202 50-51 -3.798556992832438 2202 52-53 -0.03878956274571266 2202 54-55 1.554540476460943 2202 56-57 1.4155601870759398 2202 58-59 1.487921987524281 2202 60-61 1.397641208063277 2202 62-63 1.2192584485488318 2202 64-65 1.287237179131619 2202 66-67 1.4498448859390187 2202 68-69 1.2139599666785479 2202 70-71 1.3455095063878773 2202 72-73 1.118303947315244 2202 74-75 0.6279471644157226 2202 76-77 1.3208165589916199 2202 78-79 1.3703790601036587 2202 80-81 1.8910744563572486 2202 82-83 1.7732726770366511 2202 84-85 1.5837607134676084 2202 86-87 1.7140938719968908 2202 88-89 1.5795318794553737 2202 90-91 1.3083734155576785 2202 92-93 1.3720902106045365 2202 94-95 1.3071031009189582 2202 96-97 1.384303817707739 2202 98-99 1.4044324904650551 2202 100-101 1.2744092401760803 2305 1 1.3842928630120959 2305 2 0.694967178020903 2305 3 0.08377936063818936 2305 4 -0.14236725197132927 2305 5 0.3196337387591939 2305 6 0.43010262550212985 2305 7 0.5653505290725533 2305 8 -0.04662419544216334 2305 9 0.5553787447457381 2305 10-11 0.420258474352714 2305 12-13 0.8383990630769063 2305 14-15 0.6255728027397254 2305 16-17 0.6456744238787948 2305 18-19 0.6463457313420591 2305 20-21 0.6451185077169157 2305 22-23 0.615620411621471 2305 24-25 0.7549989158686898 2305 26-27 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-1.511047468658667 1208 50-51 -0.32694264948027296 1208 52-53 -1.6022265398475781 1208 54-55 -0.7167389527225794 1208 56-57 -6.539429000585123 1208 58-59 -2.1532541503549396 1208 60-61 -1.581024897054725 1208 62-63 0.31488181046302444 1208 64-65 -0.12222197648246791 1208 66-67 0.8186610091424207 1208 68-69 0.651342718289623 1208 70-71 -4.385093273278024 1208 72-73 -0.7965537964601523 1208 74-75 -5.747396014374019 1208 76-77 -1.3671627216980582 1208 78-79 -3.9779526735076054 1208 80-81 0.731772263703899 1208 82-83 0.18712943903292256 1208 84-85 -2.902709298776198 1208 86-87 -0.97713719044933 1208 88-89 -4.977716507472142 1208 90-91 -0.12192305939438697 1208 92-93 -2.0936106641784242 1208 94-95 -4.808389866521928 1208 96-97 -3.5289337211688974 1208 98-99 -6.279274125760654 1208 100-101 -3.9152303465310183 1209 1 1.8602600368596462 1209 2 1.2973980918988062 1209 3 0.425212200030991 1209 4 0.19484248258799397 1209 5 0.4030596188264184 1209 6 1.229363360893764 1209 7 0.7110184391755467 1209 8 0.31448649281170304 1209 9 -0.3625045412256185 1209 10-11 -3.2374457624526976 1209 12-13 -0.3895535435548112 1209 14-15 -1.7172805419883304 1209 16-17 -0.6939938027180972 1209 18-19 -16.984136489949485 1209 20-21 -14.13490778889026 1209 22-23 -3.4914706448061352 1209 24-25 -2.2073878729537597 1209 26-27 -1.661159678693437 1209 28-29 -3.233245419188094 1209 30-31 -0.809617378994794 1209 32-33 -0.7267921596194853 1209 34-35 -3.632779935926898 1209 36-37 -4.4704985911254695 1209 38-39 -1.438504100118017 1209 40-41 -10.070487378158752 1209 42-43 -4.938400429788306 1209 44-45 -3.0547500693492395 1209 46-47 -2.9649180715453554 1209 48-49 -3.550526813646872 1209 50-51 -3.0499364479040025 1209 52-53 -1.2703683657488227 1209 54-55 -3.0441334430927363 1209 56-57 -1.296394376294824 1209 58-59 -2.222153097666581 1209 60-61 -2.079601963422764 1209 62-63 -1.4256764855097401 1209 64-65 -0.2772260667745421 1209 66-67 -0.5114753253651294 1209 68-69 -1.2316957972361458 1209 70-71 -1.1680310404794056 1209 72-73 -0.6669416229290306 1209 74-75 0.03134679550895214 1209 76-77 -16.703654419753597 1209 78-79 -16.71383956055775 1209 80-81 -21.854240093216145 1209 82-83 -10.47878969518645 1209 84-85 -3.8205602451940166 1209 86-87 -2.492879999048064 1209 88-89 -1.9148464066724458 1209 90-91 -6.2660361851866 1209 92-93 -3.931350639752253 1209 94-95 -1.0240904879637895 1209 96-97 -0.1825264359541805 1209 98-99 0.9914017548721645 1209 100-101 0.027200558354600446 1212 1 -28.631186308396966 1212 2 -16.315501227378146 1212 3 -5.719439853611007 1212 4 -1.3940913062603855 1212 5 -0.4189070843834344 1212 6 -2.2180718174454768 1212 7 -0.3667643668311342 1212 8 -1.0222371769738814 1212 9 0.0557274272622621 1212 10-11 0.9522396580913721 1212 12-13 0.35812877931697784 1212 14-15 0.013318373188994315 1212 16-17 -0.06852532758870922 1212 18-19 -0.8550134307750241 1212 20-21 -0.6755288849308485 1212 22-23 -0.9440566593894104 1212 24-25 0.1733915804281807 1212 26-27 -0.5771305376282498 1212 28-29 0.21937007427237631 1212 30-31 -0.800937044752132 1212 32-33 0.11756653935815109 1212 34-35 -1.4265202158646915 1212 36-37 -1.5518977383095205 1212 38-39 -0.6684791785158879 1212 40-41 -0.7663569891013111 1212 42-43 -0.4646971816940777 1212 44-45 0.7037741065169598 1212 46-47 -2.179588832323468 1212 48-49 -1.7021191510509475 1212 50-51 -0.2672642912612204 1212 52-53 -1.3573270698255158 1212 54-55 -0.7202992131766166 1212 56-57 -1.649674817617516 1212 58-59 -0.5727971712968483 1212 60-61 -0.06524654695793686 1212 62-63 -4.821059137270186 1212 64-65 -4.105742657805315 1212 66-67 -1.5892214819569226 1212 68-69 -0.3360470388545842 1212 70-71 -1.1752350406833685 1212 72-73 -1.522101541854152 1212 74-75 -0.4898668418976726 1212 76-77 0.687839410397082 1212 78-79 -1.6249830541711958 1212 80-81 0.18578584819578126 1212 82-83 0.1932076023175675 1212 84-85 -3.883498444719809 1212 86-87 -2.0671285266976582 1212 88-89 -0.986698381279183 1212 90-91 -0.13592677871590908 1212 92-93 -2.7913442978100242 1212 94-95 -2.1754406205390637 1212 96-97 0.012183141334652703 1212 98-99 -0.9123473209251998 1212 100-101 -0.12683613567696028 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 8.0 10 6.0 11 15.0 12 68.0 13 258.0 14 799.0 15 1753.0 16 3371.0 17 5824.0 18 8839.0 19 12860.0 20 18310.0 21 24360.0 22 31678.0 23 40499.0 24 51281.0 25 62863.0 26 77210.0 27 92986.0 28 111247.0 29 130433.0 30 153816.0 31 179473.0 32 205508.0 33 232621.0 34 259752.0 35 284785.0 36 297325.0 37 266766.0 38 97047.0 39 277.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.50572851753692 29.906922273659838 12.963380108344403 22.623969100458837 2 22.97409146287891 25.87878579915482 31.792549198090637 19.354573539875634 3 20.910952943323544 25.179145381133587 31.350781060287265 22.5591206152556 4 23.964635485854835 25.02106871057983 28.71524793960265 22.29904786396268 5 22.222976358944948 27.635792686306647 27.432138064334648 22.709092890413753 6 22.156499206836703 27.857659710132467 27.16596550804871 22.819875574982117 7 23.389060266459126 27.92983059449729 27.612112793213072 21.06899634583051 8 21.16522419165027 27.02614855965542 28.97702484767381 22.831602401020497 9 22.01788887719977 26.735164905191816 28.22635715387293 23.02058906373549 10-11 22.477026167413072 27.0348588388029 27.620992753123165 22.867122240660866 12-13 22.69004884688742 26.931713150766857 27.351140154241993 23.02709784810373 14-15 22.44735088737383 27.390321183059523 27.25325683370418 22.909071095862465 16-17 22.638938955395645 27.367508940327927 27.1845357590493 22.809016345227125 18-19 22.945615976375816 27.27046210545033 26.89153737280614 22.892384545367715 20-21 22.577217487604347 27.35793616872455 27.168534695103407 22.896311648567696 22-23 22.616579167953414 27.126486450614035 27.282950967161497 22.973983414271057 24-25 22.792990600816957 26.94292957230267 27.229746621373213 23.03433320550716 26-27 22.668407214222714 27.19812943927295 27.259063686469165 22.874399660035166 28-29 22.92400300297243 27.131350632475616 27.153182890598515 22.79146347395344 30-31 22.986558644122503 27.025601810531448 26.969795693049765 23.01804385229629 32-33 22.6840366978012 27.104654192491136 27.251823973931 22.95948513577666 34-35 22.950624029284636 27.049866159585136 27.113232497712136 22.88627731341809 36-37 22.97782951155696 27.009293603902506 27.0794094656979 22.933467418842636 38-39 22.741219114801854 27.08468842275698 27.309477726383356 22.864614736057803 40-41 22.982684238965874 27.08405842132115 27.073519114230628 22.85973822548235 42-43 22.792990600816957 26.975960760758046 27.259553875338938 22.971494763086064 44-45 22.702776995360928 26.984105437363475 27.389095710885098 22.924021856390496 46-47 22.91423833272957 27.05953990375359 27.198370988263477 22.82785077525337 48-49 22.984143886378657 26.910239185954044 27.162837471724565 22.942779455942734 50-51 22.77981038726081 27.070757308438758 27.238873364134815 22.910558940165615 52-53 22.88911668037626 27.073298981103385 27.24482760506034 22.792756733460013 54-55 22.771773774354674 26.90053716458322 27.329292202563334 22.998396858498772 56-57 22.717277752440644 27.104697202824497 27.305770764073877 22.872254280660982 58-59 22.82888077159117 26.986932559749327 27.319751831109883 22.86443483754962 60-61 22.947900134351283 26.87642133134842 27.12530868177751 23.050369852522792 62-63 22.708715668112234 27.036459419377778 27.331649834823835 22.923175077686153 64-65 22.93424349313732 26.96412522150831 27.204680563282984 22.89695072207138 66-67 22.96028442525852 26.85674605935381 27.20398183295314 22.978987682434532 68-69 22.794257528824822 27.030614936435786 27.302059315791233 22.873068218948163 70-71 23.079050673208737 27.034176752432675 27.008252564562067 22.878520009796517 72-73 22.876238827285313 26.897916312639676 27.26731774313191 22.95852711694311 74-75 22.7197469841386 26.940708083779818 27.40794544949057 22.931599482591015 76-77 22.884688524825446 27.139879903629822 27.21326415649466 22.76216741505008 78-79 23.03563833317245 26.927597404556202 27.068074593368408 22.96868966890294 80-81 22.764403823716123 26.98622707887335 27.318039651772352 22.93132944563818 82-83 22.93242049061902 27.01151668281525 27.15405408615707 22.902008740408654 84-85 22.881793414217974 26.832112522035878 27.206274687255483 23.07981937649066 86-87 22.72524072920809 27.0314140044621 27.325751195232233 22.91759407109758 88-89 22.914505461603234 26.990033851494623 27.14673418111021 22.948726505791935 90-91 22.96754299352127 26.86873502232367 27.016899457880715 23.14682252627434 92-93 22.72643109991029 26.973601594998698 27.293366855356894 23.006600449734123 94-95 22.90908775500545 26.96330463269844 27.05733923373585 23.070268378560264 96-97 22.925283057574255 26.937876006179195 27.02249772466001 23.114343211586537 98-99 22.77134733677169 26.953052008793428 27.18753835905185 23.088062295383036 100-101 22.9752794785581 26.851964985797917 26.999340806207584 23.1734147294364 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 2.5 2 6.0 3 11.5 4 19.5 5 35.5 6 62.0 7 95.5 8 140.5 9 208.5 10 312.5 11 424.0 12 596.0 13 868.5 14 1190.5 15 1615.5 16 2208.5 17 3027.0 18 4108.5 19 5558.5 20 7432.5 21 9802.0 22 12631.5 23 15994.5 24 20016.5 25 24484.5 26 29314.5 27 34415.5 28 39686.0 29 45341.5 30 50808.0 31 56201.0 32 61092.5 33 65947.0 34 70530.5 35 74172.5 36 77798.5 37 80088.0 38 80998.5 39 81138.5 40 80128.5 41 77849.5 42 74482.5 43 71255.0 44 68082.0 45 64501.5 46 61722.5 47 59487.5 48 57485.0 49 55272.0 50 52896.5 51 50851.0 52 48840.5 53 47351.0 54 46585.0 55 46256.5 56 45975.0 57 45840.5 58 45810.0 59 46017.0 60 46147.5 61 46021.5 62 45855.0 63 45216.0 64 44206.5 65 42763.5 66 40658.0 67 38179.5 68 35298.0 69 31919.5 70 28234.5 71 24338.5 72 20386.5 73 16447.0 74 12738.0 75 9510.0 76 6714.5 77 4412.5 78 2854.5 79 1785.0 80 1099.5 81 719.0 82 455.5 83 302.0 84 205.5 85 148.0 86 107.5 87 76.0 88 53.5 89 41.0 90 29.5 91 18.0 92 8.5 93 4.0 94 2.5 95 3.0 96 2.5 97 1.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7355849593463746 2 0.0021115828236311757 3 1.1312050840881299E-4 4 7.5413672272542E-5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.1920974766962326 14-15 0.0 16-17 4.1477519749898097E-4 18-19 0.12171766704788278 20-21 1.88534180681355E-5 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.002337823840448802 42-43 0.0 44-45 0.0 46-47 0.023208557641874798 48-49 7.5413672272542E-5 50-51 7.5413672272542E-5 52-53 1.3197392647694848E-4 54-55 0.0022058499139718537 56-57 9.238174853386393E-4 58-59 0.09626555265589985 60-61 0.001357446100905756 62-63 8.295503949979619E-4 64-65 0.0019041952248816853 66-67 0.003563296014877609 68-69 0.004317432737603029 70-71 0.0028468661282884606 72-73 0.037461741701385234 74-75 0.04291037952307639 76-77 0.16334601414232597 78-79 2.6394785295389696E-4 80-81 0.12271689820549396 82-83 0.003902657540104048 84-85 0.004298579319534894 86-87 0.005599465166236243 88-89 0.006108507454075901 90-91 0.022360153828808703 92-93 0.009426709034067749 94-95 0.01340478024644434 96-97 0.008955373582364362 98-99 0.01076530171690537 100-101 0.011877653382925365 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2652039.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.3294445931413 #Duplication Level Percentage of deduplicated Percentage of total 1 98.69877397638818 97.04995627122238 2 1.0884083381140826 2.140451747546034 3 0.1062428266659532 0.31340394414205686 4 0.07022606592447159 0.2762116023325847 5 0.017902683891706943 0.08801804818990605 6 0.01494139078313311 0.08815071942927355 7 0.0018323143893433072 0.012611931935690377 8 0.0016056892963258692 0.01263092293574997 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 6.671454681285964E-5 0.01856481226632827 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.50827344545084E-4 0.0 3.7706836136271E-5 2 1.88534180681355E-4 0.0 3.7706836136271E-5 3 2.2624101681762598E-4 3.7706836136271E-5 3.7706836136271E-5 4 2.6394785295389696E-4 7.5413672272542E-5 7.5413672272542E-5 5 3.01654689090168E-4 7.5413672272542E-5 7.5413672272542E-5 6 3.7706836136271E-4 7.5413672272542E-5 7.5413672272542E-5 7 3.7706836136271E-4 7.5413672272542E-5 7.5413672272542E-5 8 4.1477519749898097E-4 7.5413672272542E-5 7.5413672272542E-5 9 4.1477519749898097E-4 7.5413672272542E-5 1.1312050840881299E-4 10-11 4.5248203363525195E-4 7.5413672272542E-5 1.1312050840881299E-4 12-13 6.03309378180336E-4 7.5413672272542E-5 1.1312050840881299E-4 14-15 7.729901407935555E-4 7.5413672272542E-5 1.1312050840881299E-4 16-17 8.484038130660975E-4 7.5413672272542E-5 1.696807626132195E-4 18-19 8.672572311342329E-4 7.5413672272542E-5 1.88534180681355E-4 20-21 8.672572311342329E-4 1.1312050840881299E-4 1.88534180681355E-4 22-23 8.672572311342329E-4 1.50827344545084E-4 1.88534180681355E-4 24-25 8.861106492023684E-4 1.50827344545084E-4 1.88534180681355E-4 26-27 9.049640672705039E-4 1.50827344545084E-4 1.88534180681355E-4 28-29 0.0010557914118155879 1.50827344545084E-4 1.88534180681355E-4 30-31 0.0012820324286332138 1.50827344545084E-4 2.4509443488576147E-4 32-33 0.001545980281587111 1.50827344545084E-4 3.01654689090168E-4 34-35 0.0020927294055630404 1.50827344545084E-4 3.01654689090168E-4 36-37 0.0026583319476071055 1.50827344545084E-4 3.7706836136271E-4 38-39 0.003506735760673203 1.50827344545084E-4 4.1477519749898097E-4 40-41 0.004279725901466759 1.50827344545084E-4 4.3362861556711646E-4 42-43 0.005637172002372514 1.50827344545084E-4 4.5248203363525195E-4 44-45 0.007880728752480639 1.50827344545084E-4 4.5248203363525195E-4 46-47 0.010312819683270118 1.50827344545084E-4 4.7133545170338745E-4 48-49 0.013951529370420268 1.50827344545084E-4 4.901888697715229E-4 50-51 0.019249339847566344 1.50827344545084E-4 5.278957059077939E-4 52-53 0.025112752866756485 1.50827344545084E-4 5.278957059077939E-4 54-55 0.03233361198685238 1.50827344545084E-4 5.656025420440649E-4 56-57 0.04038402150194624 1.50827344545084E-4 6.41016214316607E-4 58-59 0.0510927629646472 1.50827344545084E-4 6.78723050452878E-4 60-61 0.06413932826779697 1.50827344545084E-4 7.16429886589149E-4 62-63 0.07856219308992063 1.50827344545084E-4 7.16429886589149E-4 64-65 0.09537944200669748 1.50827344545084E-4 7.16429886589149E-4 66-67 0.11417629982062857 1.50827344545084E-4 7.16429886589149E-4 68-69 0.13913822534283998 1.50827344545084E-4 7.352833046572844E-4 70-71 0.1666076554681134 1.50827344545084E-4 7.5413672272542E-4 72-73 0.19954457683314614 1.50827344545084E-4 7.5413672272542E-4 74-75 0.2376661881669161 1.696807626132195E-4 7.91843558861691E-4 76-77 0.2812741441585135 1.88534180681355E-4 8.295503949979619E-4 78-79 0.32859622350953366 1.88534180681355E-4 8.295503949979619E-4 80-81 0.38540157214882587 1.88534180681355E-4 9.238174853386395E-4 82-83 0.4467883013786751 1.88534180681355E-4 9.426709034067749E-4 84-85 0.5170172836824798 1.88534180681355E-4 9.615243214749103E-4 86-87 0.5982755155561438 1.88534180681355E-4 9.803777395430459E-4 88-89 0.6909212119429616 1.88534180681355E-4 9.803777395430459E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATGA 840 0.0058985436 6.2599554 1 GTTACAA 1205 1.9580245E-4 5.950618 1 CGTCTGA 1845 0.0 5.6589003 92-93 GATCGGA 3715 0.0 5.3652763 94-95 GTTGATG 2285 1.0456188E-6 5.0209146 1 >>END_MODULE